Click here to switch to the map view.

The map label for this gene is hybB [C]

Identifier: 116623298

GI number: 116623298

Start: 5328974

End: 5330143

Strand: Direct

Name: hybB [C]

Synonym: Acid_4205

Alternate gene names: 116623298

Gene position: 5328974-5330143 (Clockwise)

Preceding gene: 116623297

Following gene: 116623299

Centisome position: 53.47

GC content: 60.34

Gene sequence:

>1170_bases
ATGACACTCCGTCTTCCCAAACTCACCGTCTGGCGCGCGATCTTCGCCGCCATCATGCTCAGCGGCGCGTATGCCACCTA
CCTGAGAATCGTGTATGGCCTGGGCGGCTCCACGAACCTCAGCGATAAATTCCCCTGGGGCATCTGGATCGCCTTCGATG
TCATGTGCGGCGTCGGCCTTGCCGCCGGGGGCTTCACTCTCGTCGCCATCACCCACATCTTCAATATCGAAAAGTATAAG
CCCGTCCTGCGGCCCGCCATCCTTACCGCCTTTCTGGGATACACGCTGGTCGTCGTCGGCTTGCTCTTCGACCTCGGCCG
CCCGGACCGGCTTTGGCATCCCCTGGTCATGTGGAACCCGCACTCCGTGATGTTCGAAGTGGCGTGGTGCGTCACTTTGT
ACAGCACCGTGCTTTTCCTCGAGTTCCTGCCCATGGTCTTCGAGAAATTCGGCTGGCACCGTCCGCTCGAGTGGATCCAC
CGCATTTCGGTCCCGCTCATGATTTTGGGCGTGTTGCTCTCCACCCTGCACCAAAGTTCTCTCGGAACCCTCTTCCTCAT
CGTTCCCGAGAAGCTCTACCCGCTGTGGTACACGCCCATCCTGCCGCTGCTTTTCTATGTCTCGGCGATCGCGGTTGGTC
TGGCCATGACGATCTTTGAATCCTGGCACAGCAGCCGCGCCTTCGGCCGCGCGCTCGAATTGCCCCTGCTGGCCAGCATG
GGGCGCGTACTGGCGGTCATCCTCAGCGTTTATCTGTGGATCCGCTTCCTCGATATGAGCCACCGCCACGTCTTCGGACT
GCTCGCGCAAAACCGCATCGAGACCTGGCTCTTCCTGCTGGAAATCGCCCTCATGGTGACGCCGATGGTGCTGCTGTTCC
AGTCCAGGATCCGCACCAATCCGGGAGCGCTCTATGCCTGCGCCGTGATGGTAGTTTTCGGCTTCATCACCAACCGTCTC
AATGTGGGCACCACCGGTCTCGAAGCCGGCAGCGGCACGCACTACGTGCCCAAGTGGAGTGAAGTTGCGGTCACCCTCTC
CATCGTCGCCGCCGGTTTTGCAATCTTCCGCGTGATTGCCCATTATTTCCCTGTCTTTGAGGCGCACCAGTCGGAACATG
CGCCTGACCTGCAGGAACAAACGGAGGAAGACCCCGTTGCGGTCGGATGA

Upstream 100 bases:

>100_bases
AACTCGTTCCCGATATCGTCTCCACCGGTTCCGTCCTGCTCGGCGGAATCTGGTGGATCACCAATCGCCGCGCCGAAGTC
GCCAAAAAAGAGGGCCGCCG

Downstream 100 bases:

>100_bases
AACTGCTGACTGAGTTGCGCGTTGGTCTGGCCGCCAAGCTCGCGATCTGCGTGATCGCGAGCACCGCGGCCTTCTTTGCT
TTATTCGGCTATATCAACCT

Product: polysulfide reductase NrfD

Products: NA

Alternate protein names: HMC operon ORF 3 [H]

Number of amino acids: Translated: 389; Mature: 388

Protein sequence:

>389_residues
MTLRLPKLTVWRAIFAAIMLSGAYATYLRIVYGLGGSTNLSDKFPWGIWIAFDVMCGVGLAAGGFTLVAITHIFNIEKYK
PVLRPAILTAFLGYTLVVVGLLFDLGRPDRLWHPLVMWNPHSVMFEVAWCVTLYSTVLFLEFLPMVFEKFGWHRPLEWIH
RISVPLMILGVLLSTLHQSSLGTLFLIVPEKLYPLWYTPILPLLFYVSAIAVGLAMTIFESWHSSRAFGRALELPLLASM
GRVLAVILSVYLWIRFLDMSHRHVFGLLAQNRIETWLFLLEIALMVTPMVLLFQSRIRTNPGALYACAVMVVFGFITNRL
NVGTTGLEAGSGTHYVPKWSEVAVTLSIVAAGFAIFRVIAHYFPVFEAHQSEHAPDLQEQTEEDPVAVG

Sequences:

>Translated_389_residues
MTLRLPKLTVWRAIFAAIMLSGAYATYLRIVYGLGGSTNLSDKFPWGIWIAFDVMCGVGLAAGGFTLVAITHIFNIEKYK
PVLRPAILTAFLGYTLVVVGLLFDLGRPDRLWHPLVMWNPHSVMFEVAWCVTLYSTVLFLEFLPMVFEKFGWHRPLEWIH
RISVPLMILGVLLSTLHQSSLGTLFLIVPEKLYPLWYTPILPLLFYVSAIAVGLAMTIFESWHSSRAFGRALELPLLASM
GRVLAVILSVYLWIRFLDMSHRHVFGLLAQNRIETWLFLLEIALMVTPMVLLFQSRIRTNPGALYACAVMVVFGFITNRL
NVGTTGLEAGSGTHYVPKWSEVAVTLSIVAAGFAIFRVIAHYFPVFEAHQSEHAPDLQEQTEEDPVAVG
>Mature_388_residues
TLRLPKLTVWRAIFAAIMLSGAYATYLRIVYGLGGSTNLSDKFPWGIWIAFDVMCGVGLAAGGFTLVAITHIFNIEKYKP
VLRPAILTAFLGYTLVVVGLLFDLGRPDRLWHPLVMWNPHSVMFEVAWCVTLYSTVLFLEFLPMVFEKFGWHRPLEWIHR
ISVPLMILGVLLSTLHQSSLGTLFLIVPEKLYPLWYTPILPLLFYVSAIAVGLAMTIFESWHSSRAFGRALELPLLASMG
RVLAVILSVYLWIRFLDMSHRHVFGLLAQNRIETWLFLLEIALMVTPMVLLFQSRIRTNPGALYACAVMVVFGFITNRLN
VGTTGLEAGSGTHYVPKWSEVAVTLSIVAAGFAIFRVIAHYFPVFEAHQSEHAPDLQEQTEEDPVAVG

Specific function: HMWC (high-molecular-weight cytochrome c), ORF2, ORF3, ORF4, ORF5 and ORF6 in the HMC operon form a transmembrane protein complex that allows electron flow from the periplasmic hydrogenase to the cytoplasmic enzymes that catalyze reduction of sulfates [H]

COG id: COG5557

COG function: function code C; Polysulphide reductase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nrfD family [H]

Homologues:

Organism=Escherichia coli, GI2367183, Length=352, Percent_Identity=32.3863636363636, Blast_Score=192, Evalue=3e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005614 [H]

Pfam domain/function: PF03916 NrfD [H]

EC number: NA

Molecular weight: Translated: 43700; Mature: 43569

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRLPKLTVWRAIFAAIMLSGAYATYLRIVYGLGGSTNLSDKFPWGIWIAFDVMCGVGL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
AAGGFTLVAITHIFNIEKYKPVLRPAILTAFLGYTLVVVGLLFDLGRPDRLWHPLVMWNP
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCEEEECC
HSVMFEVAWCVTLYSTVLFLEFLPMVFEKFGWHRPLEWIHRISVPLMILGVLLSTLHQSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
LGTLFLIVPEKLYPLWYTPILPLLFYVSAIAVGLAMTIFESWHSSRAFGRALELPLLASM
CCEEEEEECCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRVLAVILSVYLWIRFLDMSHRHVFGLLAQNRIETWLFLLEIALMVTPMVLLFQSRIRTN
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PGALYACAVMVVFGFITNRLNVGTTGLEAGSGTHYVPKWSEVAVTLSIVAAGFAIFRVIA
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
HYFPVFEAHQSEHAPDLQEQTEEDPVAVG
HHHHHHHHHCCCCCCCHHHHCCCCCCCCC
>Mature Secondary Structure 
TLRLPKLTVWRAIFAAIMLSGAYATYLRIVYGLGGSTNLSDKFPWGIWIAFDVMCGVGL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHH
AAGGFTLVAITHIFNIEKYKPVLRPAILTAFLGYTLVVVGLLFDLGRPDRLWHPLVMWNP
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCEEEECC
HSVMFEVAWCVTLYSTVLFLEFLPMVFEKFGWHRPLEWIHRISVPLMILGVLLSTLHQSS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
LGTLFLIVPEKLYPLWYTPILPLLFYVSAIAVGLAMTIFESWHSSRAFGRALELPLLASM
CCEEEEEECCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GRVLAVILSVYLWIRFLDMSHRHVFGLLAQNRIETWLFLLEIALMVTPMVLLFQSRIRTN
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PGALYACAVMVVFGFITNRLNVGTTGLEAGSGTHYVPKWSEVAVTLSIVAAGFAIFRVIA
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
HYFPVFEAHQSEHAPDLQEQTEEDPVAVG
HHHHHHHHHCCCCCCCHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8335628 [H]