Click here to switch to the map view.

The map label for this gene is gcvH [H]

Identifier: 116623296

GI number: 116623296

Start: 5327495

End: 5328166

Strand: Direct

Name: gcvH [H]

Synonym: Acid_4203

Alternate gene names: 116623296

Gene position: 5327495-5328166 (Clockwise)

Preceding gene: 116623295

Following gene: 116623297

Centisome position: 53.46

GC content: 60.57

Gene sequence:

>672_bases
ATGACAGTCGCTCTCGTACTTCTCACCTTCCTGGTCTTCATCGTTCTGGACTACGCCCTCAATCGACGCAAGGCCATTCA
CACCGTAGCAGTTGAAACTCCCAAAACAGTCCCGGCCCACCTGGGCGGCGACTATGTCGATGGCTTCCTCGTTCCCCGCA
ACGTCTCCTATCACTCCGGCCACAGCTGGCTGGTCCGCGAACGCAAGAACGTCGTCCGCGTGGGCGCCGATGAATTCGCC
GCCGCGCTCGCCGGCAAACTCGAAAAGATCGAGCTTCCCAAGCCCGGCCAGTGGGTCCGCCAGGGCCAGAGAGTCATCTC
CTTTTTCCGCGACGGCCAGAAGACCGAAATGGTCAGCCCGACCGAAGGCGAAATCATGGAGATCAATAACGAAGTCCTCA
ACAACCCGGCCCTCGTCCGCCAGGACCCCTACGGCAAAGGCTGGCTGATCAGCGTTCATGTACCGGATGAAGAGAATACC
TCCCGCAACCTGATTCCAAAAGCGTTAATCGGAGAGTGGATGCGCGAAGCGGTGGAACGCCTCTATTCGCGTCAGCCGGC
CCTCGCCGGAGCCGTTGCAGCCGATGGCGGACGCCCCTCCGAAGACCTGCTCGCCGCCTTGCCGGAACACGATTGGAAGG
AAGTCACCGCCGAGTTCTTCCTGACCGTGTAG

Upstream 100 bases:

>100_bases
CAGCCTCTATCTCGCTGATTCTACGGCTGATATCGCTCGAACATCGGCCTCCATTTGGCATCCGCCGTGCCCTTACTGAA
AGCGAAAGGCGGTAAACAAA

Downstream 100 bases:

>100_bases
GTTTCTCTGTAAAGATTCCCTACACTTTGGCTCAGTTTTCTGTTAAAAAGGAATTGAAGGTCTTATTATGTCGAAGGCGA
TACTGTACGACAGCACCCTC

Product: glycine cleavage H-protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 223; Mature: 222

Protein sequence:

>223_residues
MTVALVLLTFLVFIVLDYALNRRKAIHTVAVETPKTVPAHLGGDYVDGFLVPRNVSYHSGHSWLVRERKNVVRVGADEFA
AALAGKLEKIELPKPGQWVRQGQRVISFFRDGQKTEMVSPTEGEIMEINNEVLNNPALVRQDPYGKGWLISVHVPDEENT
SRNLIPKALIGEWMREAVERLYSRQPALAGAVAADGGRPSEDLLAALPEHDWKEVTAEFFLTV

Sequences:

>Translated_223_residues
MTVALVLLTFLVFIVLDYALNRRKAIHTVAVETPKTVPAHLGGDYVDGFLVPRNVSYHSGHSWLVRERKNVVRVGADEFA
AALAGKLEKIELPKPGQWVRQGQRVISFFRDGQKTEMVSPTEGEIMEINNEVLNNPALVRQDPYGKGWLISVHVPDEENT
SRNLIPKALIGEWMREAVERLYSRQPALAGAVAADGGRPSEDLLAALPEHDWKEVTAEFFLTV
>Mature_222_residues
TVALVLLTFLVFIVLDYALNRRKAIHTVAVETPKTVPAHLGGDYVDGFLVPRNVSYHSGHSWLVRERKNVVRVGADEFAA
ALAGKLEKIELPKPGQWVRQGQRVISFFRDGQKTEMVSPTEGEIMEINNEVLNNPALVRQDPYGKGWLISVHVPDEENTS
RNLIPKALIGEWMREAVERLYSRQPALAGAVAADGGRPSEDLLAALPEHDWKEVTAEFFLTV

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789271, Length=107, Percent_Identity=29.9065420560748, Blast_Score=67, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319272, Length=103, Percent_Identity=31.0679611650485, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 24877; Mature: 24746

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVALVLLTFLVFIVLDYALNRRKAIHTVAVETPKTVPAHLGGDYVDGFLVPRNVSYHSG
CHHHHHHHHHHHHHHHHHHHCCHHHEEEEEECCCCCCCHHCCCCHHCCEEECCCCCCCCC
HSWLVRERKNVVRVGADEFAAALAGKLEKIELPKPGQWVRQGQRVISFFRDGQKTEMVSP
CHHHHHHCCCCEEECHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
TEGEIMEINNEVLNNPALVRQDPYGKGWLISVHVPDEENTSRNLIPKALIGEWMREAVER
CCCCEEECCHHHHCCCCEEECCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
LYSRQPALAGAVAADGGRPSEDLLAALPEHDWKEVTAEFFLTV
HHCCCCCHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TVALVLLTFLVFIVLDYALNRRKAIHTVAVETPKTVPAHLGGDYVDGFLVPRNVSYHSG
HHHHHHHHHHHHHHHHHHHCCHHHEEEEEECCCCCCCHHCCCCHHCCEEECCCCCCCCC
HSWLVRERKNVVRVGADEFAAALAGKLEKIELPKPGQWVRQGQRVISFFRDGQKTEMVSP
CHHHHHHCCCCEEECHHHHHHHHHCCHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
TEGEIMEINNEVLNNPALVRQDPYGKGWLISVHVPDEENTSRNLIPKALIGEWMREAVER
CCCCEEECCHHHHCCCCEEECCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHH
LYSRQPALAGAVAADGGRPSEDLLAALPEHDWKEVTAEFFLTV
HHCCCCCHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA