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The map label for this gene is leuB [H]

Identifier: 116623162

GI number: 116623162

Start: 5164352

End: 5165437

Strand: Direct

Name: leuB [H]

Synonym: Acid_4068

Alternate gene names: 116623162

Gene position: 5164352-5165437 (Clockwise)

Preceding gene: 116623161

Following gene: 116623163

Centisome position: 51.82

GC content: 61.79

Gene sequence:

>1086_bases
ATGAACCTCAATGTCCTCGTTCTCCCCGGTGACGGCATCGGAACCGAAGTCACTTGTCAGGCCGTCCGGGTATTGGAACA
CGTCGCGAAGAAGTGGAACCACACCCTCCATCTCACCGAGGGCCTGCTGGGCGGCATCGCGATTCACAAGACCGGAACGC
CGTTTCCGCAAGAGACAGCGAAACTCGCCGCCTCCGCCGACGCCACGCTGATGGGCGCGGTGGGTCTTCCCGAATTTGAC
AATGCACCACCCGAGAAGCGCCCCGAAAAAGGACTCCTCGGAATTCGACAGGCTCTGGGCGTGTACGCAAACCTCCGTCC
GGTTCGGGCCTACGCGTCGCAACTGGATTCTTCACCCCTGAAGAACCACCTGGTGGAAGGCACCGATATGATCATCGTCC
GCGAACTGACGGGCGGGATCTATTACGGAACTCCGCGCGGAATCTCCGGCAGCGGATTGGAAGAGCGGGCGGTCAATACC
ATGACCTACACACGCGCCGAAATCGAGCGCGTTGCCCGCATGGCCTTCCACCTGGCGCGCGCCCGCCGCAAGAAAGTGAC
CTCGGTCGATAAATCGAACGTGCTGGAGAATTCGCAGCTCTGGCGGCGCGTAGTCGTTGAAGTCGCCAAAAACTTCCCGG
ATATCGAGCTGGATCACATCCTGGTGGACAACTGCGCGATGCAGCTGGTGCTCAACCCCAAGCGCTTTGATATCGTGCTC
ACGGAAAATATGTTCGGCGATATCTTGAGCGATGAAGGCGCGGTACTGGCCGGTTCCATCGGCATGCTCCCCTCCGCCTC
CATCGGCGACCAGCGTCCGTCGGGCGCGTGGGTGGGTCTGTATGAGCCGGTCCACGGCTCCGCCCCCGATATCGCGGGAC
AGAATAAGGCCAACCCGCTCGGCGCCATCGGCTCCGTAGCGGCGATGCTGGCTTACAGTTTTGGTTTGAAGGAAGAAGCC
GCCGCGGTGGAAACAGCCATCGAAGCGGTCCTCACAAGTGGCCAGGTCACAGCCGACCTGAGGCCTGCAGGCGCGCCCGC
CACCACCGCACAGGTTGGTCAAGCGGTCTGCGCCGCAATCGGCTGA

Upstream 100 bases:

>100_bases
GGTGGCCGACCATCGCAAGAAGGGCCTCATGGACGAAGAAATCGTCGCCCTCATCAAGGAGTGCCAGCAGAAGGCGCTCG
GCGTGGCGGCGGACTAATTC

Downstream 100 bases:

>100_bases
GCACGCGGATTACCATTCCGCGCGCTCAGCCATAATAGAAATAGCCCCCACCGAGTTTTCTTATGCCACGTACGATCATC
GAAAAGCTTTGGGATTCCCA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MNLNVLVLPGDGIGTEVTCQAVRVLEHVAKKWNHTLHLTEGLLGGIAIHKTGTPFPQETAKLAASADATLMGAVGLPEFD
NAPPEKRPEKGLLGIRQALGVYANLRPVRAYASQLDSSPLKNHLVEGTDMIIVRELTGGIYYGTPRGISGSGLEERAVNT
MTYTRAEIERVARMAFHLARARRKKVTSVDKSNVLENSQLWRRVVVEVAKNFPDIELDHILVDNCAMQLVLNPKRFDIVL
TENMFGDILSDEGAVLAGSIGMLPSASIGDQRPSGAWVGLYEPVHGSAPDIAGQNKANPLGAIGSVAAMLAYSFGLKEEA
AAVETAIEAVLTSGQVTADLRPAGAPATTAQVGQAVCAAIG

Sequences:

>Translated_361_residues
MNLNVLVLPGDGIGTEVTCQAVRVLEHVAKKWNHTLHLTEGLLGGIAIHKTGTPFPQETAKLAASADATLMGAVGLPEFD
NAPPEKRPEKGLLGIRQALGVYANLRPVRAYASQLDSSPLKNHLVEGTDMIIVRELTGGIYYGTPRGISGSGLEERAVNT
MTYTRAEIERVARMAFHLARARRKKVTSVDKSNVLENSQLWRRVVVEVAKNFPDIELDHILVDNCAMQLVLNPKRFDIVL
TENMFGDILSDEGAVLAGSIGMLPSASIGDQRPSGAWVGLYEPVHGSAPDIAGQNKANPLGAIGSVAAMLAYSFGLKEEA
AAVETAIEAVLTSGQVTADLRPAGAPATTAQVGQAVCAAIG
>Mature_361_residues
MNLNVLVLPGDGIGTEVTCQAVRVLEHVAKKWNHTLHLTEGLLGGIAIHKTGTPFPQETAKLAASADATLMGAVGLPEFD
NAPPEKRPEKGLLGIRQALGVYANLRPVRAYASQLDSSPLKNHLVEGTDMIIVRELTGGIYYGTPRGISGSGLEERAVNT
MTYTRAEIERVARMAFHLARARRKKVTSVDKSNVLENSQLWRRVVVEVAKNFPDIELDHILVDNCAMQLVLNPKRFDIVL
TENMFGDILSDEGAVLAGSIGMLPSASIGDQRPSGAWVGLYEPVHGSAPDIAGQNKANPLGAIGSVAAMLAYSFGLKEEA
AAVETAIEAVLTSGQVTADLRPAGAPATTAQVGQAVCAAIG

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=364, Percent_Identity=29.9450549450549, Blast_Score=131, Evalue=1e-30,
Organism=Homo sapiens, GI28178838, Length=350, Percent_Identity=25.7142857142857, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI4758582, Length=365, Percent_Identity=26.027397260274, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI28178816, Length=366, Percent_Identity=27.0491803278689, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI28178821, Length=317, Percent_Identity=26.813880126183, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI28178819, Length=149, Percent_Identity=33.5570469798658, Blast_Score=89, Evalue=7e-18,
Organism=Escherichia coli, GI87081683, Length=357, Percent_Identity=46.7787114845938, Blast_Score=327, Evalue=6e-91,
Organism=Escherichia coli, GI1788101, Length=365, Percent_Identity=35.0684931506849, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1787381, Length=385, Percent_Identity=27.2727272727273, Blast_Score=99, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI71986051, Length=364, Percent_Identity=31.8681318681319, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI25144293, Length=376, Percent_Identity=29.2553191489362, Blast_Score=123, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17505779, Length=351, Percent_Identity=27.9202279202279, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17550882, Length=359, Percent_Identity=27.8551532033426, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319830, Length=363, Percent_Identity=43.801652892562, Blast_Score=280, Evalue=2e-76,
Organism=Saccharomyces cerevisiae, GI6322097, Length=368, Percent_Identity=32.6086956521739, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6324709, Length=362, Percent_Identity=31.2154696132597, Blast_Score=133, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6324291, Length=371, Percent_Identity=29.6495956873315, Blast_Score=132, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24643268, Length=370, Percent_Identity=30.8108108108108, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24643270, Length=370, Percent_Identity=30.8108108108108, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24661184, Length=352, Percent_Identity=30.6818181818182, Blast_Score=124, Evalue=9e-29,
Organism=Drosophila melanogaster, GI161078633, Length=252, Percent_Identity=29.7619047619048, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24650122, Length=252, Percent_Identity=29.7619047619048, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161078635, Length=252, Percent_Identity=29.7619047619048, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161078637, Length=252, Percent_Identity=29.7619047619048, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI161078639, Length=252, Percent_Identity=29.7619047619048, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI281362242, Length=365, Percent_Identity=27.3972602739726, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI24648872, Length=365, Percent_Identity=27.3972602739726, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI20130355, Length=294, Percent_Identity=23.469387755102, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38227; Mature: 38227

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNVLVLPGDGIGTEVTCQAVRVLEHVAKKWNHTLHLTEGLLGGIAIHKTGTPFPQETA
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEEEHHHHHCCEEEECCCCCCCHHHH
KLAASADATLMGAVGLPEFDNAPPEKRPEKGLLGIRQALGVYANLRPVRAYASQLDSSPL
HHHHCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHH
KNHLVEGTDMIIVRELTGGIYYGTPRGISGSGLEERAVNTMTYTRAEIERVARMAFHLAR
HHHHCCCCCEEEEEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARRKKVTSVDKSNVLENSQLWRRVVVEVAKNFPDIELDHILVDNCAMQLVLNPKRFDIVL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEHHCHHEEEEECCCEEEEEE
TENMFGDILSDEGAVLAGSIGMLPSASIGDQRPSGAWVGLYEPVHGSAPDIAGQNKANPL
ECCHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
GAIGSVAAMLAYSFGLKEEAAAVETAIEAVLTSGQVTADLRPAGAPATTAQVGQAVCAAI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHC
G
C
>Mature Secondary Structure
MNLNVLVLPGDGIGTEVTCQAVRVLEHVAKKWNHTLHLTEGLLGGIAIHKTGTPFPQETA
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCEEEEHHHHHCCEEEECCCCCCCHHHH
KLAASADATLMGAVGLPEFDNAPPEKRPEKGLLGIRQALGVYANLRPVRAYASQLDSSPL
HHHHCCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHH
KNHLVEGTDMIIVRELTGGIYYGTPRGISGSGLEERAVNTMTYTRAEIERVARMAFHLAR
HHHHCCCCCEEEEEECCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARRKKVTSVDKSNVLENSQLWRRVVVEVAKNFPDIELDHILVDNCAMQLVLNPKRFDIVL
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEHHCHHEEEEECCCEEEEEE
TENMFGDILSDEGAVLAGSIGMLPSASIGDQRPSGAWVGLYEPVHGSAPDIAGQNKANPL
ECCHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
GAIGSVAAMLAYSFGLKEEAAAVETAIEAVLTSGQVTADLRPAGAPATTAQVGQAVCAAI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA