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The map label for this gene is leuA [H]

Identifier: 116623161

GI number: 116623161

Start: 5163173

End: 5164348

Strand: Direct

Name: leuA [H]

Synonym: Acid_4067

Alternate gene names: 116623161

Gene position: 5163173-5164348 (Clockwise)

Preceding gene: 116623155

Following gene: 116623162

Centisome position: 51.81

GC content: 63.35

Gene sequence:

>1176_bases
GTGAGCGATCGAGTATACATTTTCGACACGACTCTGCGCGATGGCGAACAATCGCCGGGCTGCAGCATGACCGTTCCAGA
GAAATTGACCATGGCCCGCAAGCTGGTCGAGCTCGGCGTGGACATTCTGGAAGCCGGATTCCCGATTGCCAGCGAAGGCG
ATTTCGACGCGGTGGACGCCGTCAGCCGCGAGTTTCCCTGGGCGCAGGTGGCGGCGCTGGCGCGCTGCTGCACACTCGAT
GTCGAGCGCGCCGCGAAATCCCTGGCGCACGCCAAGCGCCCGCGCATCCACACCTTCATCGCCACCAGCGATATCCACCT
CAAGTTCAAGCTCAAGAAGACGCAGCAACAGGTGCTCGATGAAGCCGTCGCAGCCGTGGAACTGGCCCGCCGTCACGTGG
ACGACGTGGAGTTCTCCGCCGAAGATGGCGCGCGCACCGAACCTGAATTCCTCGAGAAGATCTCCAAAGCCGTGGTCGCC
GCCGGCGCGCGCACGGTCAATATCCCGGACACAGTCGGCTACTCGACCCCCAAGGAATACGGCGCGCTGATCGGCCGCAT
CGCCAAGGCGCTGGGCGATTGCGCCATCGTCAGCGTGCACTGCCATGACGACCTGGGTCTCGCGGTGGCCAACTCCCTCG
CCGCGATCGAAGCCGGCGCCCGGCAGATCGAGTGCACCATCAACGGCATCGGCGAACGCGCGGGCAACTGTTCGCTCGAA
GAGATCGTCATGATCCTCAAGACGCGCGCGGATTCGTACCCCTATCACACCGGCATTCACACCGAGCATCTCTATTCCGC
CAGCCAGCTGCTGACTTCGACCATCACCTTCGGGCCGCAGCCCAACAAGGCGATCGTGGGCCGCAACGCCTTCGCGCATG
AAGCCGGCATTCACCAGGATGGCTACCTGAAAGAAAAGACCACCTACGAAATCATCGACCCGCGCACCGTCGGCATTCCC
GAAGGCAGCCTGGTGCTGGGCAAGCACAGCGGCCGTCACGCGCTGAATCAGCGCGCCATCGAGATGGGCTACCAACTTTC
CAAGCCCGAGCTCGACGACCTGTATCACCGCTTCACCGCGGTGGCCGACCATCGCAAGAAGGGCCTCATGGACGAAGAAA
TCGTCGCCCTCATCAAGGAGTGCCAGCAGAAGGCGCTCGGCGTGGCGGCGGACTAA

Upstream 100 bases:

>100_bases
TGAAGGCTAAGCGGGACTCCATTCACTATAACATACGTTAATATGGACCGCTAATATTATAAATTTGCCAAATCGAACAC
GGGACCGCGATAATGCTCTT

Downstream 100 bases:

>100_bases
TTCATGAACCTCAATGTCCTCGTTCTCCCCGGTGACGGCATCGGAACCGAAGTCACTTGTCAGGCCGTCCGGGTATTGGA
ACACGTCGCGAAGAAGTGGA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 391; Mature: 390

Protein sequence:

>391_residues
MSDRVYIFDTTLRDGEQSPGCSMTVPEKLTMARKLVELGVDILEAGFPIASEGDFDAVDAVSREFPWAQVAALARCCTLD
VERAAKSLAHAKRPRIHTFIATSDIHLKFKLKKTQQQVLDEAVAAVELARRHVDDVEFSAEDGARTEPEFLEKISKAVVA
AGARTVNIPDTVGYSTPKEYGALIGRIAKALGDCAIVSVHCHDDLGLAVANSLAAIEAGARQIECTINGIGERAGNCSLE
EIVMILKTRADSYPYHTGIHTEHLYSASQLLTSTITFGPQPNKAIVGRNAFAHEAGIHQDGYLKEKTTYEIIDPRTVGIP
EGSLVLGKHSGRHALNQRAIEMGYQLSKPELDDLYHRFTAVADHRKKGLMDEEIVALIKECQQKALGVAAD

Sequences:

>Translated_391_residues
MSDRVYIFDTTLRDGEQSPGCSMTVPEKLTMARKLVELGVDILEAGFPIASEGDFDAVDAVSREFPWAQVAALARCCTLD
VERAAKSLAHAKRPRIHTFIATSDIHLKFKLKKTQQQVLDEAVAAVELARRHVDDVEFSAEDGARTEPEFLEKISKAVVA
AGARTVNIPDTVGYSTPKEYGALIGRIAKALGDCAIVSVHCHDDLGLAVANSLAAIEAGARQIECTINGIGERAGNCSLE
EIVMILKTRADSYPYHTGIHTEHLYSASQLLTSTITFGPQPNKAIVGRNAFAHEAGIHQDGYLKEKTTYEIIDPRTVGIP
EGSLVLGKHSGRHALNQRAIEMGYQLSKPELDDLYHRFTAVADHRKKGLMDEEIVALIKECQQKALGVAAD
>Mature_390_residues
SDRVYIFDTTLRDGEQSPGCSMTVPEKLTMARKLVELGVDILEAGFPIASEGDFDAVDAVSREFPWAQVAALARCCTLDV
ERAAKSLAHAKRPRIHTFIATSDIHLKFKLKKTQQQVLDEAVAAVELARRHVDDVEFSAEDGARTEPEFLEKISKAVVAA
GARTVNIPDTVGYSTPKEYGALIGRIAKALGDCAIVSVHCHDDLGLAVANSLAAIEAGARQIECTINGIGERAGNCSLEE
IVMILKTRADSYPYHTGIHTEHLYSASQLLTSTITFGPQPNKAIVGRNAFAHEAGIHQDGYLKEKTTYEIIDPRTVGIPE
GSLVLGKHSGRHALNQRAIEMGYQLSKPELDDLYHRFTAVADHRKKGLMDEEIVALIKECQQKALGVAAD

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=294, Percent_Identity=21.7687074829932, Blast_Score=66, Evalue=7e-11,
Organism=Escherichia coli, GI1786261, Length=389, Percent_Identity=48.8431876606684, Blast_Score=365, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6320019, Length=365, Percent_Identity=28.2191780821918, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6320071, Length=374, Percent_Identity=27.807486631016, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6324682, Length=409, Percent_Identity=27.1393643031785, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324225, Length=264, Percent_Identity=31.4393939393939, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 42569; Mature: 42438

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRVYIFDTTLRDGEQSPGCSMTVPEKLTMARKLVELGVDILEAGFPIASEGDFDAVDA
CCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
VSREFPWAQVAALARCCTLDVERAAKSLAHAKRPRIHTFIATSDIHLKFKLKKTQQQVLD
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEEEHHHHHHHHH
EAVAAVELARRHVDDVEFSAEDGARTEPEFLEKISKAVVAAGARTVNIPDTVGYSTPKEY
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHH
GALIGRIAKALGDCAIVSVHCHDDLGLAVANSLAAIEAGARQIECTINGIGERAGNCSLE
HHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHH
EIVMILKTRADSYPYHTGIHTEHLYSASQLLTSTITFGPQPNKAIVGRNAFAHEAGIHQD
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHCCCCCC
GYLKEKTTYEIIDPRTVGIPEGSLVLGKHSGRHALNQRAIEMGYQLSKPELDDLYHRFTA
CCCCCCCEEEEECCEEECCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
VADHRKKGLMDEEIVALIKECQQKALGVAAD
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SDRVYIFDTTLRDGEQSPGCSMTVPEKLTMARKLVELGVDILEAGFPIASEGDFDAVDA
CCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHH
VSREFPWAQVAALARCCTLDVERAAKSLAHAKRPRIHTFIATSDIHLKFKLKKTQQQVLD
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEEEEEHHHHHHHHH
EAVAAVELARRHVDDVEFSAEDGARTEPEFLEKISKAVVAAGARTVNIPDTVGYSTPKEY
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHH
GALIGRIAKALGDCAIVSVHCHDDLGLAVANSLAAIEAGARQIECTINGIGERAGNCSLE
HHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCHH
EIVMILKTRADSYPYHTGIHTEHLYSASQLLTSTITFGPQPNKAIVGRNAFAHEAGIHQD
HHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHCCCCCC
GYLKEKTTYEIIDPRTVGIPEGSLVLGKHSGRHALNQRAIEMGYQLSKPELDDLYHRFTA
CCCCCCCEEEEECCEEECCCCCCEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
VADHRKKGLMDEEIVALIKECQQKALGVAAD
HHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA