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The map label for this gene is gudD [H]

Identifier: 116623077

GI number: 116623077

Start: 5047757

End: 5049082

Strand: Reverse

Name: gudD [H]

Synonym: Acid_3981

Alternate gene names: 116623077

Gene position: 5049082-5047757 (Counterclockwise)

Preceding gene: 116623078

Following gene: 116623072

Centisome position: 50.66

GC content: 66.14

Gene sequence:

>1326_bases
ATGAGCGCACCTATCGTCGAAGAAATGCGCGTGATCCCGGTGGCGGGGCACGACAGCATGCTGCTCAATTTGAGCGGCGC
GCATGCTCCGCTGTTCACGCGAAATCTGGTGATCCTCAAGGACAGCGCGGGGAACACAGGCGTCGGTGAGGTGCCGGGCG
GCGAAGCGATTCGCGCCACGCTGGAAGAGGCTATGCCGCTGGTAGCGGGCCGCTCGATCGGCGCGTACACCAACATCCTG
GATTCCGTGAGCCGGCAGTTCGCCGATCGCGATGCCGGCGGTCGCGGGCTGCAGACCTTCGATCAGCGGACCACGATCCA
CGCGGTGACGGCGCTCGAGGCGGCGCTGCTGGATCTACTCGGGAAGCATCTGGGCGTGCCGGTGGCGGCGCTGTTGGGCG
AAGGGCAGCAGCGCAAGACTGTGGATGTGCTGGGGTATCTTTTTTACGTCGGTGACCGCGGGAAGACCGATCTGCCTTAC
GCGTCGGAGCCGGAGTCGATTGACGACTGGCTGCGGCTGCGGCACGAGGAGGCATTGAGCGTCGATGCGGTGGTGCGGTT
GGCCGAGGCCGCGCAGGCGCGGTATGGCTTTCGCGATTTCAAGCTGAAGGGCGGCGTGCGCGCGGGCGGGGAAGAGATGG
AGGCGATTGTCGCGCTGGCGGAGCGGTTTCCGGAGGCGCGGATCACGATCGATCCGAACGGCGCGTGGTCGCTGCGCCAG
GCGATCGCGCTGTGCAAGGGGCGCGGCGACGTGCTGGCCTATGCCGAAGACCCATGCGGCGCGGAAGGTGGCTATTCGGG
GCGCGAAGTGATGGCGGAGTTCCGGCGCGCGACGGGCCTGCCGACGGCGAGCAACATGATCGCGACGGACTGGCGGCAAC
TGGGGCACGCGATCCAGTTGCACTCGGTGGATATCCCGTTGGCCGATCCGCATTTCTGGACGATGCGGGGGTCGGTGCGG
GTGGCGCAGTTATGCCACGAGTGGGGGCTGACGTGGGGCTCACATTCCAACAATCACTTCGATGTGTCGCTGGCGATGTT
CACGCACGTGGCGGCGGCGGCTCCGGGGAAGATTACGGCGATCGATACGCACTGGATCTGGCAGGACGGTCAACGGCTGA
CGAAGGAGCCATTCCGGATCGAGGGCGGGAAGATCGCGGTGCCGGAGGCTCCGGGGCTCGGCGTGGAGCTCGATATGGTT
GAGGTGGGGAAGGCGCACGCGGTGTACCGGGGAATGGGGCTGGGAGCGCGGGATGATGCGCAGGCGATGCAGTATTTGAT
CCCCGGCTGGAAGTTCGACAATAAGCGGCCGTCTCTGGTGAGGTAG

Upstream 100 bases:

>100_bases
TGATCGCGCTCATGAATGACGGTAAGGGCAATCTGGATCACTCGGCGATCGTAAACTTCATCGAGCATATGGCAGGCATC
GAAGTGAAGAAGCCGCAGGC

Downstream 100 bases:

>100_bases
GCGTCGCCGACGAAGCCGCGACCGTGAGGAAGCGGTCGGTAATCAACCGGCAAATTAGCGACCGCTCCCTGACGGTCGGT
GCTCCGATGAGGTGTCTTCT

Product: D-glucarate dehydratase

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MSAPIVEEMRVIPVAGHDSMLLNLSGAHAPLFTRNLVILKDSAGNTGVGEVPGGEAIRATLEEAMPLVAGRSIGAYTNIL
DSVSRQFADRDAGGRGLQTFDQRTTIHAVTALEAALLDLLGKHLGVPVAALLGEGQQRKTVDVLGYLFYVGDRGKTDLPY
ASEPESIDDWLRLRHEEALSVDAVVRLAEAAQARYGFRDFKLKGGVRAGGEEMEAIVALAERFPEARITIDPNGAWSLRQ
AIALCKGRGDVLAYAEDPCGAEGGYSGREVMAEFRRATGLPTASNMIATDWRQLGHAIQLHSVDIPLADPHFWTMRGSVR
VAQLCHEWGLTWGSHSNNHFDVSLAMFTHVAAAAPGKITAIDTHWIWQDGQRLTKEPFRIEGGKIAVPEAPGLGVELDMV
EVGKAHAVYRGMGLGARDDAQAMQYLIPGWKFDNKRPSLVR

Sequences:

>Translated_441_residues
MSAPIVEEMRVIPVAGHDSMLLNLSGAHAPLFTRNLVILKDSAGNTGVGEVPGGEAIRATLEEAMPLVAGRSIGAYTNIL
DSVSRQFADRDAGGRGLQTFDQRTTIHAVTALEAALLDLLGKHLGVPVAALLGEGQQRKTVDVLGYLFYVGDRGKTDLPY
ASEPESIDDWLRLRHEEALSVDAVVRLAEAAQARYGFRDFKLKGGVRAGGEEMEAIVALAERFPEARITIDPNGAWSLRQ
AIALCKGRGDVLAYAEDPCGAEGGYSGREVMAEFRRATGLPTASNMIATDWRQLGHAIQLHSVDIPLADPHFWTMRGSVR
VAQLCHEWGLTWGSHSNNHFDVSLAMFTHVAAAAPGKITAIDTHWIWQDGQRLTKEPFRIEGGKIAVPEAPGLGVELDMV
EVGKAHAVYRGMGLGARDDAQAMQYLIPGWKFDNKRPSLVR
>Mature_440_residues
SAPIVEEMRVIPVAGHDSMLLNLSGAHAPLFTRNLVILKDSAGNTGVGEVPGGEAIRATLEEAMPLVAGRSIGAYTNILD
SVSRQFADRDAGGRGLQTFDQRTTIHAVTALEAALLDLLGKHLGVPVAALLGEGQQRKTVDVLGYLFYVGDRGKTDLPYA
SEPESIDDWLRLRHEEALSVDAVVRLAEAAQARYGFRDFKLKGGVRAGGEEMEAIVALAERFPEARITIDPNGAWSLRQA
IALCKGRGDVLAYAEDPCGAEGGYSGREVMAEFRRATGLPTASNMIATDWRQLGHAIQLHSVDIPLADPHFWTMRGSVRV
AQLCHEWGLTWGSHSNNHFDVSLAMFTHVAAAAPGKITAIDTHWIWQDGQRLTKEPFRIEGGKIAVPEAPGLGVELDMVE
VGKAHAVYRGMGLGARDDAQAMQYLIPGWKFDNKRPSLVR

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc). Also acts on L-idarate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=442, Percent_Identity=74.4343891402715, Blast_Score=698, Evalue=0.0,
Organism=Escherichia coli, GI1789151, Length=443, Percent_Identity=64.1083521444695, Blast_Score=571, Evalue=1e-164,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017653
- InterPro:   IPR013342
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 47783; Mature: 47652

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPIVEEMRVIPVAGHDSMLLNLSGAHAPLFTRNLVILKDSAGNTGVGEVPGGEAIRAT
CCCCCCCCCEEEEECCCCCEEEEECCCCCCEEECCEEEEECCCCCCCCCCCCCCHHHHHH
LEEAMPLVAGRSIGAYTNILDSVSRQFADRDAGGRGLQTFDQRTTIHAVTALEAALLDLL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GKHLGVPVAALLGEGQQRKTVDVLGYLFYVGDRGKTDLPYASEPESIDDWLRLRHEEALS
HHHHCCCHHHHHCCCCCCHHHHHHEEHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VDAVVRLAEAAQARYGFRDFKLKGGVRAGGEEMEAIVALAERFPEARITIDPNGAWSLRQ
HHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHH
AIALCKGRGDVLAYAEDPCGAEGGYSGREVMAEFRRATGLPTASNMIATDWRQLGHAIQL
HHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEE
HSVDIPLADPHFWTMRGSVRVAQLCHEWGLTWGSHSNNHFDVSLAMFTHVAAAAPGKITA
EEECCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHCCCCCEEE
IDTHWIWQDGQRLTKEPFRIEGGKIAVPEAPGLGVELDMVEVGKAHAVYRGMGLGARDDA
EEEEEEECCCHHHCCCCEEECCCEEECCCCCCCCEEEEHHHHCCHHHHHHCCCCCCCCHH
QAMQYLIPGWKFDNKRPSLVR
HHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SAPIVEEMRVIPVAGHDSMLLNLSGAHAPLFTRNLVILKDSAGNTGVGEVPGGEAIRAT
CCCCCCCCEEEEECCCCCEEEEECCCCCCEEECCEEEEECCCCCCCCCCCCCCHHHHHH
LEEAMPLVAGRSIGAYTNILDSVSRQFADRDAGGRGLQTFDQRTTIHAVTALEAALLDLL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
GKHLGVPVAALLGEGQQRKTVDVLGYLFYVGDRGKTDLPYASEPESIDDWLRLRHEEALS
HHHHCCCHHHHHCCCCCCHHHHHHEEHHEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
VDAVVRLAEAAQARYGFRDFKLKGGVRAGGEEMEAIVALAERFPEARITIDPNGAWSLRQ
HHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHH
AIALCKGRGDVLAYAEDPCGAEGGYSGREVMAEFRRATGLPTASNMIATDWRQLGHAIQL
HHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCEEEE
HSVDIPLADPHFWTMRGSVRVAQLCHEWGLTWGSHSNNHFDVSLAMFTHVAAAAPGKITA
EEECCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHCCCCCEEE
IDTHWIWQDGQRLTKEPFRIEGGKIAVPEAPGLGVELDMVEVGKAHAVYRGMGLGARDDA
EEEEEEECCCHHHCCCCEEECCCEEECCCCCCCCEEEEHHHHCCHHHHHHCCCCCCCCHH
QAMQYLIPGWKFDNKRPSLVR
HHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]