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The map label for this gene is yfnG [H]

Identifier: 116621368

GI number: 116621368

Start: 2873821

End: 2874801

Strand: Reverse

Name: yfnG [H]

Synonym: Acid_2249

Alternate gene names: 116621368

Gene position: 2874801-2873821 (Counterclockwise)

Preceding gene: 116621369

Following gene: 116621367

Centisome position: 28.85

GC content: 62.9

Gene sequence:

>981_bases
GTGAGCGGTTTTTGGCAGGACCGCCCCGTCCTGGTGACCGGCGCCACCGGCCTTGTCGGCTCCTGGCTCACGCGCCGCCT
TCTCGACGCCGGCGCCGACGTGGTCTGCCTGGTGCGCGATTGGGTTCCGCAGTCCGAGCTCGTCCGCTCCGGCACGCTCG
ATAGAGTCAAAGTCGTGCGCGGCGATATCCGCGACCTCGACGCCTTGGAGCGCACCATCGGGGAATACGAAACCGGCACC
GTGATCCACCTGGCTGCGCAGACCATCGTAGGCATCGCCAACCGCAATCCGATTTCCACCTTCGAGAGCAACATCCAGGG
AACCTGGAATCTGCTGGAGGCCTGCCGCCGCGCGCCGCTGGTCAAATCCATCGTCATCGCATCTTCCGACAAGGCCTATG
GGGATCAGGAAAAGCTGCCGTACTCCGAGGACGCGCCCCTCCAGGGGCAGCACCCGTACGATGTCAGCAAGAGCTGCGCC
GACCTCATCTCGCAAACCTACGCCAAAAGCTACGGCCTGCCGGTCGCGGTCACGCGTTGTGGCAATTTCTACGGCGGCGG
CGATCTCAACTGGAACCGGATCGTGCCCGGCACCATCCGCTCTATCCTGCGCGGAGAGCGCCCCGTGATCCGCTCCGATG
GCCAGTTTATCCGCGACTATTTCTATGTAGAGGATGGCGCCGCGGCTTACATGCTGCTCGCCGAACTGCTCCACTCGCGG
CCGGAATTGCGCGGCTGCGCGTTTAATTTTTCCAATGAAATCCAGGTGACCGCGCTGGATCTCGTGCGCCGCATCCTGTG
CGCCATGGGCTCCACACTGCAGCCCGACGTCCGCAATGAGGCCGTCAATGAGATCCGCCATCAGTACCTGACGGCGGAAC
GCGCAAAAACCATGCTGAACTGGTCGCCGCTATTTACCCTGGAGCAGGGCCTGCAACACACCATAGCCTGGTATAAGGAG
TTTCTCCGTGACCGGGCGTAA

Upstream 100 bases:

>100_bases
ACCGCCACGACGGATTTTTTTACGCCATGGATACTTTCCGCGAATATCAGCACCTTAACGAACTCTGGAGCAAGGGCGCC
GCTCCCTGGCGGGTGTGGGC

Downstream 100 bases:

>100_bases
AGAGGAGCTGCGCAAACAAATACTGGATCTGGTGGGCGAGTACTGCGCGGAGGCCTTTCCGCCGCGTCCCTTCATCCCCG
GCGAATCGCCCGTGCCCGTG

Product: NAD-dependent epimerase/dehydratase

Products: CDP-4-dehydro-6-deoxy-D-glucose; H2O

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MSGFWQDRPVLVTGATGLVGSWLTRRLLDAGADVVCLVRDWVPQSELVRSGTLDRVKVVRGDIRDLDALERTIGEYETGT
VIHLAAQTIVGIANRNPISTFESNIQGTWNLLEACRRAPLVKSIVIASSDKAYGDQEKLPYSEDAPLQGQHPYDVSKSCA
DLISQTYAKSYGLPVAVTRCGNFYGGGDLNWNRIVPGTIRSILRGERPVIRSDGQFIRDYFYVEDGAAAYMLLAELLHSR
PELRGCAFNFSNEIQVTALDLVRRILCAMGSTLQPDVRNEAVNEIRHQYLTAERAKTMLNWSPLFTLEQGLQHTIAWYKE
FLRDRA

Sequences:

>Translated_326_residues
MSGFWQDRPVLVTGATGLVGSWLTRRLLDAGADVVCLVRDWVPQSELVRSGTLDRVKVVRGDIRDLDALERTIGEYETGT
VIHLAAQTIVGIANRNPISTFESNIQGTWNLLEACRRAPLVKSIVIASSDKAYGDQEKLPYSEDAPLQGQHPYDVSKSCA
DLISQTYAKSYGLPVAVTRCGNFYGGGDLNWNRIVPGTIRSILRGERPVIRSDGQFIRDYFYVEDGAAAYMLLAELLHSR
PELRGCAFNFSNEIQVTALDLVRRILCAMGSTLQPDVRNEAVNEIRHQYLTAERAKTMLNWSPLFTLEQGLQHTIAWYKE
FLRDRA
>Mature_325_residues
SGFWQDRPVLVTGATGLVGSWLTRRLLDAGADVVCLVRDWVPQSELVRSGTLDRVKVVRGDIRDLDALERTIGEYETGTV
IHLAAQTIVGIANRNPISTFESNIQGTWNLLEACRRAPLVKSIVIASSDKAYGDQEKLPYSEDAPLQGQHPYDVSKSCAD
LISQTYAKSYGLPVAVTRCGNFYGGGDLNWNRIVPGTIRSILRGERPVIRSDGQFIRDYFYVEDGAAAYMLLAELLHSRP
ELRGCAFNFSNEIQVTALDLVRRILCAMGSTLQPDVRNEAVNEIRHQYLTAERAKTMLNWSPLFTLEQGLQHTIAWYKEF
LRDRA

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI42516563, Length=335, Percent_Identity=26.865671641791, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI7657641, Length=327, Percent_Identity=23.8532110091743, Blast_Score=103, Evalue=2e-22,
Organism=Homo sapiens, GI56237023, Length=340, Percent_Identity=26.1764705882353, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI56118217, Length=340, Percent_Identity=26.1764705882353, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI189083684, Length=340, Percent_Identity=26.1764705882353, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI48994969, Length=338, Percent_Identity=28.698224852071, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1788353, Length=340, Percent_Identity=28.5294117647059, Blast_Score=117, Evalue=7e-28,
Organism=Escherichia coli, GI1786974, Length=332, Percent_Identity=28.0120481927711, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788366, Length=172, Percent_Identity=31.3953488372093, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17568069, Length=343, Percent_Identity=26.2390670553936, Blast_Score=104, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI115532424, Length=336, Percent_Identity=25.8928571428571, Blast_Score=98, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI71982038, Length=331, Percent_Identity=23.8670694864048, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71982035, Length=329, Percent_Identity=24.3161094224924, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17539532, Length=330, Percent_Identity=23.3333333333333, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17507723, Length=193, Percent_Identity=26.9430051813472, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319493, Length=341, Percent_Identity=25.5131964809384, Blast_Score=79, Evalue=7e-16,
Organism=Drosophila melanogaster, GI21356223, Length=322, Percent_Identity=27.0186335403727, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19923002, Length=338, Percent_Identity=26.6272189349112, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013445
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.45

Molecular weight: Translated: 36481; Mature: 36350

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGFWQDRPVLVTGATGLVGSWLTRRLLDAGADVVCLVRDWVPQSELVRSGTLDRVKVVR
CCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCEEEEEHHCCCHHHHHHCCCHHHHHHHH
GDIRDLDALERTIGEYETGTVIHLAAQTIVGIANRNPISTFESNIQGTWNLLEACRRAPL
CCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHCCH
VKSIVIASSDKAYGDQEKLPYSEDAPLQGQHPYDVSKSCADLISQTYAKSYGLPVAVTRC
HHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEC
GNFYGGGDLNWNRIVPGTIRSILRGERPVIRSDGQFIRDYFYVEDGAAAYMLLAELLHSR
CCCCCCCCCCCCCCCCHHHHHHHCCCCCEECCCCCEEHHHHEECCCHHHHHHHHHHHHCC
PELRGCAFNFSNEIQVTALDLVRRILCAMGSTLQPDVRNEAVNEIRHQYLTAERAKTMLN
CCCCEEEECCCCCEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
WSPLFTLEQGLQHTIAWYKEFLRDRA
CCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SGFWQDRPVLVTGATGLVGSWLTRRLLDAGADVVCLVRDWVPQSELVRSGTLDRVKVVR
CCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCEEEEEHHCCCHHHHHHCCCHHHHHHHH
GDIRDLDALERTIGEYETGTVIHLAAQTIVGIANRNPISTFESNIQGTWNLLEACRRAPL
CCHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHCCH
VKSIVIASSDKAYGDQEKLPYSEDAPLQGQHPYDVSKSCADLISQTYAKSYGLPVAVTRC
HHHHHEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEC
GNFYGGGDLNWNRIVPGTIRSILRGERPVIRSDGQFIRDYFYVEDGAAAYMLLAELLHSR
CCCCCCCCCCCCCCCCHHHHHHHCCCCCEECCCCCEEHHHHEECCCHHHHHHHHHHHHCC
PELRGCAFNFSNEIQVTALDLVRRILCAMGSTLQPDVRNEAVNEIRHQYLTAERAKTMLN
CCCCEEEECCCCCEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
WSPLFTLEQGLQHTIAWYKEFLRDRA
CCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: CDPglucose

Specific reaction: CDP-glucose = CDP-4-dehydro-6-deoxy-D-glucose + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9141694; 9384377 [H]