Click here to switch to the map view.

The map label for this gene is rfbF [H]

Identifier: 116621369

GI number: 116621369

Start: 2874798

End: 2875574

Strand: Reverse

Name: rfbF [H]

Synonym: Acid_2250

Alternate gene names: 116621369

Gene position: 2875574-2874798 (Counterclockwise)

Preceding gene: 116621370

Following gene: 116621368

Centisome position: 28.85

GC content: 58.69

Gene sequence:

>777_bases
GTGAAAGTCGTAATCCTGTGCGGTGGTCTGGGTACGCGTTTGCGTGAGGAGACGGAATTTCGCCCCAAGCCCATGGTGGA
GGTGGGCGGCCGTCCCATCCTCTGGCACATCATGAAGAGTTATGCCCACTATGGCCACCGGGAGTTCGTCCTCTGCCTCG
GGTACCGCGGAAATTCCATCAAAGAGTATTTCTTGAATTACGAGGCGATGAACAACGACTTCTCCATCCAACTCGGGGAA
AAGTCGCGCATCCGCTATCATGGCTTGCACGCGGAGCAGGAATTCACCGTCACCCTGGCCGATACCGGCGCCGATTGCAT
GACCGGCGGCCGCATCCGCAAAATCCAGCGCTACATTGATGACGATACCTTCCTGATGACCTATGGCGACGGTCTGATCG
ACGTCAACATCGGCAGCGTCGTGGAGTATCACAAGAGCCACGGCCGCATCGCCACGGTGACCACCGTGCCCCCGATTTCC
CGCTTCGGCGTCATCGATCTCGGCGAGAACGATTGCGTCAGCAGCTTCAACGAAAAGCCAAAAACCGACGGCTGGATGAA
CGCCGGTTACTTCGTGCTCAACACTTCGGTCTTCGACTACCTCGGCGGCGACGAGTGCATCTTTGAGCGCGAACCCCTGG
AGCGCCTCGCCCGTGACCGCCAGCTGATGGCCTACCGCCACGACGGATTTTTTTACGCCATGGATACTTTCCGCGAATAT
CAGCACCTTAACGAACTCTGGAGCAAGGGCGCCGCTCCCTGGCGGGTGTGGGCGTGA

Upstream 100 bases:

>100_bases
CATTTGCCTCACTCGGACATGTCTTCCTCGCAGTTATCCATCTCTGGCGGGCCTGTGGAATAATGATGAATTCGTTCCTT
CGTGAAGGAGTTCCTTTTCT

Downstream 100 bases:

>100_bases
GCGGTTTTTGGCAGGACCGCCCCGTCCTGGTGACCGGCGCCACCGGCCTTGTCGGCTCCTGGCTCACGCGCCGCCTTCTC
GACGCCGGCGCCGACGTGGT

Product: glucose-1-phosphate cytidylyltransferase

Products: NA

Alternate protein names: CDP-glucose pyrophosphorylase [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKVVILCGGLGTRLREETEFRPKPMVEVGGRPILWHIMKSYAHYGHREFVLCLGYRGNSIKEYFLNYEAMNNDFSIQLGE
KSRIRYHGLHAEQEFTVTLADTGADCMTGGRIRKIQRYIDDDTFLMTYGDGLIDVNIGSVVEYHKSHGRIATVTTVPPIS
RFGVIDLGENDCVSSFNEKPKTDGWMNAGYFVLNTSVFDYLGGDECIFEREPLERLARDRQLMAYRHDGFFYAMDTFREY
QHLNELWSKGAAPWRVWA

Sequences:

>Translated_258_residues
MKVVILCGGLGTRLREETEFRPKPMVEVGGRPILWHIMKSYAHYGHREFVLCLGYRGNSIKEYFLNYEAMNNDFSIQLGE
KSRIRYHGLHAEQEFTVTLADTGADCMTGGRIRKIQRYIDDDTFLMTYGDGLIDVNIGSVVEYHKSHGRIATVTTVPPIS
RFGVIDLGENDCVSSFNEKPKTDGWMNAGYFVLNTSVFDYLGGDECIFEREPLERLARDRQLMAYRHDGFFYAMDTFREY
QHLNELWSKGAAPWRVWA
>Mature_258_residues
MKVVILCGGLGTRLREETEFRPKPMVEVGGRPILWHIMKSYAHYGHREFVLCLGYRGNSIKEYFLNYEAMNNDFSIQLGE
KSRIRYHGLHAEQEFTVTLADTGADCMTGGRIRKIQRYIDDDTFLMTYGDGLIDVNIGSVVEYHKSHGRIATVTTVPPIS
RFGVIDLGENDCVSSFNEKPKTDGWMNAGYFVLNTSVFDYLGGDECIFEREPLERLARDRQLMAYRHDGFFYAMDTFREY
QHLNELWSKGAAPWRVWA

Specific function: Involved in the biosynthesis of the tyvelose, a 3,6- dideoxyhexose found in the O-antigen of the surface lipopolysaccharides. It catalyzes the transfer of a CMP moiety from CTP to glucose 1-phosphate. This enzyme can utilize either CTP or UTP as the nucle

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate cytidylyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=248, Percent_Identity=25.8064516129032, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI11761619, Length=248, Percent_Identity=25.8064516129032, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI133931050, Length=246, Percent_Identity=26.4227642276423, Blast_Score=89, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320148, Length=249, Percent_Identity=28.5140562248996, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21355443, Length=245, Percent_Identity=26.530612244898, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24644084, Length=245, Percent_Identity=26.530612244898, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013446
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.33 [H]

Molecular weight: Translated: 29754; Mature: 29754

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVVILCGGLGTRLREETEFRPKPMVEVGGRPILWHIMKSYAHYGHREFVLCLGYRGNSI
CEEEEEECCCCCHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCH
KEYFLNYEAMNNDFSIQLGEKSRIRYHGLHAEQEFTVTLADTGADCMTGGRIRKIQRYID
HHHHEEHEECCCCEEEEECCCCCEEEECCCCCCEEEEEEECCCCCHHCCCHHHHHHHHHC
DDTFLMTYGDGLIDVNIGSVVEYHKSHGRIATVTTVPPISRFGVIDLGENDCVSSFNEKP
CCEEEEEECCCEEEEEHHHHHHHHHCCCCEEEEEECCCCHHCCEEECCCCHHHHHCCCCC
KTDGWMNAGYFVLNTSVFDYLGGDECIFEREPLERLARDRQLMAYRHDGFFYAMDTFREY
CCCCCCCCCEEEEECHHHHHCCCCCEEECCCHHHHHHHCCCEEEEECCCEEEEHHHHHHH
QHLNELWSKGAAPWRVWA
HHHHHHHHCCCCCEEECC
>Mature Secondary Structure
MKVVILCGGLGTRLREETEFRPKPMVEVGGRPILWHIMKSYAHYGHREFVLCLGYRGNSI
CEEEEEECCCCCHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCH
KEYFLNYEAMNNDFSIQLGEKSRIRYHGLHAEQEFTVTLADTGADCMTGGRIRKIQRYID
HHHHEEHEECCCCEEEEECCCCCEEEECCCCCCEEEEEEECCCCCHHCCCHHHHHHHHHC
DDTFLMTYGDGLIDVNIGSVVEYHKSHGRIATVTTVPPISRFGVIDLGENDCVSSFNEKP
CCEEEEEECCCEEEEEHHHHHHHHHCCCCEEEEEECCCCHHCCEEECCCCHHHHHCCCCC
KTDGWMNAGYFVLNTSVFDYLGGDECIFEREPLERLARDRQLMAYRHDGFFYAMDTFREY
CCCCCCCCCEEEEECHHHHHCCCCCEEECCCHHHHHHHCCCEEEEECCCEEEEHHHHHHH
QHLNELWSKGAAPWRVWA
HHHHHHHHCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]