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The map label for this gene is gutB [H]

Identifier: 116620387

GI number: 116620387

Start: 1563261

End: 1564301

Strand: Direct

Name: gutB [H]

Synonym: Acid_1264

Alternate gene names: 116620387

Gene position: 1563261-1564301 (Clockwise)

Preceding gene: 116620386

Following gene: 116620388

Centisome position: 15.69

GC content: 60.33

Gene sequence:

>1041_bases
GTGAATAACCACATGATGGCTGCTGTGCTTTACGGCAAGGAGCATTTGCAGGTAGAGCCGGTAGCCGTTCCCAAGATTGA
CATCGGTGACATTCTCGTACGGGTAAAGGTGGCGTTGACCTGCGGCACGGACGTAAAAGTCTTCCGCCGGGGCTACCATG
CCCGCATGATCGTACCCCCCGCGGTGTTCGGGCACGAGTTGGCCGGCGATGTCGTCGCCGTGGGTGAGGATGTCCGGAAC
TTCAGCGTGGGTCAGCGTGTAGTGGCGGCGAACTCCGCTCCATGCGGCGAGTGCTTCTTCTGCAAGCGGGAACTGGAAAA
CCTTTGCGAAGATCTGCTGTTCAACAACGGCGCGTACGCCGAATATATTCGGATTCCTGCCCGCATCGTCCAACGGAATA
CTTATCTGATCCCCGATGATGTGGGATATCAGGACGCTGCGCTGGTGGAACCGCTGGCATGCGTAGTGCGCGGATTTGAA
GAATCGGCGCCGCGCGCCGGCGATACCGTGGCGATTGTGGGACTGGGGCCGATCGGGCTGATGTTCGTGAAACTGGCGAA
GCTGTACGGCTGCCGCGTGATCGCGGTCGGACGGCGGCAAACGCAGTTGGACCGCGCAGAGGCGTTGGGCGCGGATCATT
TGATTGTCGCGGGCGAGCATAGCGATCCCGTGAAATCGAGCCGCGAGATGACGCACGGGCGCGGCGTGGATGTGGCGATC
GAAGCAGTCGGCAAGCCGCAGACCTGGCAATGGGCCGTCAACATGGTGCGGCGCGGAGGCACGGTCAATTTCTTCGGAGG
CTGCCCCAACGAAAGCAAGGTGAATCTGGATACCGCGCTCCTCCACTACTCCGAAATCACCTGCAAGGCGAGCTTCCATC
ACACTCCGGCTTACATTCAGAAAGCGCTGGATCTGGTCTGCGCCGGGCACATCACGGCCTCGGTATTCGTCAACAAGGAA
GAGCCGTTGGCCAATCTCCTCGAAGTGATGCGGCACCTGATGAGCCACAACGGCCACCTGAAGACGGCGATCGTTCCGTA
G

Upstream 100 bases:

>100_bases
ATCGTCTGCCATTAGTTAAAATCCGTTCAGGATTTGATTTAGCATTGCATCCAGGACCTAAATCGTTGAAAATAATCGTT
CAGCCACAGAGGTGGTCATA

Downstream 100 bases:

>100_bases
AATGTCCGGCGTGGGCTCACCCGCGTTTCTGCCGCCGGCGAATTTCGTCAGGTCGCCGGAACTGCTCAATAAGCAATGGT
CGGCCGAGGAAGGACGCGCT

Product: alcohol dehydrogenase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MNNHMMAAVLYGKEHLQVEPVAVPKIDIGDILVRVKVALTCGTDVKVFRRGYHARMIVPPAVFGHELAGDVVAVGEDVRN
FSVGQRVVAANSAPCGECFFCKRELENLCEDLLFNNGAYAEYIRIPARIVQRNTYLIPDDVGYQDAALVEPLACVVRGFE
ESAPRAGDTVAIVGLGPIGLMFVKLAKLYGCRVIAVGRRQTQLDRAEALGADHLIVAGEHSDPVKSSREMTHGRGVDVAI
EAVGKPQTWQWAVNMVRRGGTVNFFGGCPNESKVNLDTALLHYSEITCKASFHHTPAYIQKALDLVCAGHITASVFVNKE
EPLANLLEVMRHLMSHNGHLKTAIVP

Sequences:

>Translated_346_residues
MNNHMMAAVLYGKEHLQVEPVAVPKIDIGDILVRVKVALTCGTDVKVFRRGYHARMIVPPAVFGHELAGDVVAVGEDVRN
FSVGQRVVAANSAPCGECFFCKRELENLCEDLLFNNGAYAEYIRIPARIVQRNTYLIPDDVGYQDAALVEPLACVVRGFE
ESAPRAGDTVAIVGLGPIGLMFVKLAKLYGCRVIAVGRRQTQLDRAEALGADHLIVAGEHSDPVKSSREMTHGRGVDVAI
EAVGKPQTWQWAVNMVRRGGTVNFFGGCPNESKVNLDTALLHYSEITCKASFHHTPAYIQKALDLVCAGHITASVFVNKE
EPLANLLEVMRHLMSHNGHLKTAIVP
>Mature_346_residues
MNNHMMAAVLYGKEHLQVEPVAVPKIDIGDILVRVKVALTCGTDVKVFRRGYHARMIVPPAVFGHELAGDVVAVGEDVRN
FSVGQRVVAANSAPCGECFFCKRELENLCEDLLFNNGAYAEYIRIPARIVQRNTYLIPDDVGYQDAALVEPLACVVRGFE
ESAPRAGDTVAIVGLGPIGLMFVKLAKLYGCRVIAVGRRQTQLDRAEALGADHLIVAGEHSDPVKSSREMTHGRGVDVAI
EAVGKPQTWQWAVNMVRRGGTVNFFGGCPNESKVNLDTALLHYSEITCKASFHHTPAYIQKALDLVCAGHITASVFVNKE
EPLANLLEVMRHLMSHNGHLKTAIVP

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=334, Percent_Identity=26.0479041916168, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI4501933, Length=303, Percent_Identity=27.3927392739274, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI34577061, Length=303, Percent_Identity=27.3927392739274, Blast_Score=75, Evalue=9e-14,
Organism=Homo sapiens, GI4501939, Length=237, Percent_Identity=27.8481012658228, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI156523966, Length=237, Percent_Identity=27.8481012658228, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI1788073, Length=291, Percent_Identity=31.9587628865979, Blast_Score=126, Evalue=2e-30,
Organism=Escherichia coli, GI1790045, Length=311, Percent_Identity=28.9389067524116, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI226510992, Length=324, Percent_Identity=28.3950617283951, Blast_Score=111, Evalue=7e-26,
Organism=Escherichia coli, GI1788075, Length=276, Percent_Identity=30.0724637681159, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI87082125, Length=297, Percent_Identity=29.6296296296296, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1787863, Length=217, Percent_Identity=32.258064516129, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1786825, Length=275, Percent_Identity=31.6363636363636, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1788407, Length=250, Percent_Identity=29.6, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1790718, Length=349, Percent_Identity=25.7879656160458, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1786518, Length=271, Percent_Identity=26.9372693726937, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081918, Length=337, Percent_Identity=26.7062314540059, Blast_Score=61, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17562876, Length=340, Percent_Identity=27.9411764705882, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17562878, Length=347, Percent_Identity=28.5302593659942, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17562584, Length=328, Percent_Identity=27.7439024390244, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17562582, Length=328, Percent_Identity=28.6585365853659, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71988145, Length=340, Percent_Identity=25.2941176470588, Blast_Score=86, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323099, Length=350, Percent_Identity=25.7142857142857, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6322619, Length=357, Percent_Identity=25.4901960784314, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6319955, Length=357, Percent_Identity=25.4901960784314, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323729, Length=336, Percent_Identity=26.7857142857143, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319257, Length=301, Percent_Identity=27.2425249169435, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6319520, Length=263, Percent_Identity=25.4752851711027, Blast_Score=73, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6323961, Length=329, Percent_Identity=26.7477203647416, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324486, Length=339, Percent_Identity=25.9587020648968, Blast_Score=70, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6319258, Length=270, Percent_Identity=24.0740740740741, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17137530, Length=360, Percent_Identity=28.0555555555556, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI17737897, Length=327, Percent_Identity=29.3577981651376, Blast_Score=108, Evalue=5e-24,
Organism=Drosophila melanogaster, GI45550770, Length=183, Percent_Identity=26.2295081967213, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45551930, Length=183, Percent_Identity=26.2295081967213, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI221457811, Length=183, Percent_Identity=26.2295081967213, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 37729; Mature: 37729

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNHMMAAVLYGKEHLQVEPVAVPKIDIGDILVRVKVALTCGTDVKVFRRGYHARMIVPP
CCCCEEEEEEECCCCCEECCEECCCCCHHHHEEEEEEEEEECCCHHHHHCCCCEEEEECC
AVFGHELAGDVVAVGEDVRNFSVGQRVVAANSAPCGECFFCKRELENLCEDLLFNNGAYA
HHHHHHHCCCEEEECCHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHH
EYIRIPARIVQRNTYLIPDDVGYQDAALVEPLACVVRGFEESAPRAGDTVAIVGLGPIGL
HHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHCHHHCCCCCCCEEEEEECCHHHH
MFVKLAKLYGCRVIAVGRRQTQLDRAEALGADHLIVAGEHSDPVKSSREMTHGRGVDVAI
HHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCCCCEEEE
EAVGKPQTWQWAVNMVRRGGTVNFFGGCPNESKVNLDTALLHYSEITCKASFHHTPAYIQ
EECCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHEEHHHEEEEECCCCCHHHHH
KALDLVCAGHITASVFVNKEEPLANLLEVMRHLMSHNGHLKTAIVP
HHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECC
>Mature Secondary Structure
MNNHMMAAVLYGKEHLQVEPVAVPKIDIGDILVRVKVALTCGTDVKVFRRGYHARMIVPP
CCCCEEEEEEECCCCCEECCEECCCCCHHHHEEEEEEEEEECCCHHHHHCCCCEEEEECC
AVFGHELAGDVVAVGEDVRNFSVGQRVVAANSAPCGECFFCKRELENLCEDLLFNNGAYA
HHHHHHHCCCEEEECCHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCHH
EYIRIPARIVQRNTYLIPDDVGYQDAALVEPLACVVRGFEESAPRAGDTVAIVGLGPIGL
HHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHHHHHHCHHHCCCCCCCEEEEEECCHHHH
MFVKLAKLYGCRVIAVGRRQTQLDRAEALGADHLIVAGEHSDPVKSSREMTHGRGVDVAI
HHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCCCCEEEE
EAVGKPQTWQWAVNMVRRGGTVNFFGGCPNESKVNLDTALLHYSEITCKASFHHTPAYIQ
EECCCCCHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHEEHHHEEEEECCCCCHHHHH
KALDLVCAGHITASVFVNKEEPLANLLEVMRHLMSHNGHLKTAIVP
HHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1460002; 9384377; 8195086 [H]