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The map label for this gene is gutB [H]

Identifier: 116620386

GI number: 116620386

Start: 1562164

End: 1563261

Strand: Direct

Name: gutB [H]

Synonym: Acid_1263

Alternate gene names: 116620386

Gene position: 1562164-1563261 (Clockwise)

Preceding gene: 116620385

Following gene: 116620387

Centisome position: 15.68

GC content: 58.93

Gene sequence:

>1098_bases
TTGCCGACTGCCGATACTGACGTGAGCGCCATACCGGAAACCATGCACGCAGCGGTGTATCGCGGCAGCAGCGTCGTCAA
CGTGGAAGAGATCCCCACCCCCTCCATCGGGCCAGGCGAGATTCTGATCCGGGTTGAGGCATGCGGCATTTGTCATACGG
ACCTCAAGAAAATTGAATACAACCTGTTGGAGCCGCCGCGGATCTACGGGCATGAAACGGCCGGCGTCGTGGCCGCGGTG
GGCAGCGGCGTCACCAGGTTTCAAATTGGCGACCGGGTCGTAGCCTTCCATCACATTCCGTGCGGCAAGTGCTTCTATTG
CCAGCGTAAGATATACGCGCAGTGTCCGATATACAAGAAAGTTGGGATCACCGCGGGGTTTGAGCCCGCGGGAGGCGGAT
TCGCCCAGTACGTTCGGGTGATGGACTGGATCGTCGAAGAGGGCGTCGAGCGCATTCCAGACGATGTTTCCTTTGAGCTG
GCCACGCTGGTAGAGCCGTTGAATACCTGTCTCAAGGCCGTGGTGCAGTGCGACCCGCAGCCGGACGATTTTGTGGTCGT
GCTGGGGCAGGGGCCGATCGGGCTGATGTTTACGATGCTGACGCGTCGCACCGGTGCGCGGGTTGCCGCCACCGATACCA
TCGACACCCGGCTGGAGCTGGCGGCGCGATGCGGCGCCGAGTTCGCCTGGGACCCGCGTACTATCGACGTGGCGGCGGAG
GTCAGGAATTTGACCGGAGGACGCGGTGCGGACCTGGTGATTGTGGCGGCCTCGGTTCCGGGAATCGTGAATCAGGCCAT
CGCCTGCTCGCGGCCCGGTTCGCGGATCCTGCTTTTCGCGCAAACTTCGGCAACCGAGCGCGTTGAGACGTCGGGAGCCG
ATATCTGCGTCGGTGAACGTACGCTTTTCGGGTGCTATAGTGCTTCAATAGACTTACAAAAAGAATCTGCGCGGCTGGTA
TTTGGCGGCGAGATCCCTGTAGAAGCATTGATATCTCATCGTCTGCCATTAGTTAAAATCCGTTCAGGATTTGATTTAGC
ATTGCATCCAGGACCTAAATCGTTGAAAATAATCGTTCAGCCACAGAGGTGGTCATAG

Upstream 100 bases:

>100_bases
ATTTTACGCAGAGCATTACGAGATCCGCTGGTTCGCGTGCCGGAACTGATCCGGTTGCGCAATCGCTGTGTTCGGGCTTC
GCGATCGCTGGGAGAATTTA

Downstream 100 bases:

>100_bases
TGAATAACCACATGATGGCTGCTGTGCTTTACGGCAAGGAGCATTTGCAGGTAGAGCCGGTAGCCGTTCCCAAGATTGAC
ATCGGTGACATTCTCGTACG

Product: alcohol dehydrogenase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MPTADTDVSAIPETMHAAVYRGSSVVNVEEIPTPSIGPGEILIRVEACGICHTDLKKIEYNLLEPPRIYGHETAGVVAAV
GSGVTRFQIGDRVVAFHHIPCGKCFYCQRKIYAQCPIYKKVGITAGFEPAGGGFAQYVRVMDWIVEEGVERIPDDVSFEL
ATLVEPLNTCLKAVVQCDPQPDDFVVVLGQGPIGLMFTMLTRRTGARVAATDTIDTRLELAARCGAEFAWDPRTIDVAAE
VRNLTGGRGADLVIVAASVPGIVNQAIACSRPGSRILLFAQTSATERVETSGADICVGERTLFGCYSASIDLQKESARLV
FGGEIPVEALISHRLPLVKIRSGFDLALHPGPKSLKIIVQPQRWS

Sequences:

>Translated_365_residues
MPTADTDVSAIPETMHAAVYRGSSVVNVEEIPTPSIGPGEILIRVEACGICHTDLKKIEYNLLEPPRIYGHETAGVVAAV
GSGVTRFQIGDRVVAFHHIPCGKCFYCQRKIYAQCPIYKKVGITAGFEPAGGGFAQYVRVMDWIVEEGVERIPDDVSFEL
ATLVEPLNTCLKAVVQCDPQPDDFVVVLGQGPIGLMFTMLTRRTGARVAATDTIDTRLELAARCGAEFAWDPRTIDVAAE
VRNLTGGRGADLVIVAASVPGIVNQAIACSRPGSRILLFAQTSATERVETSGADICVGERTLFGCYSASIDLQKESARLV
FGGEIPVEALISHRLPLVKIRSGFDLALHPGPKSLKIIVQPQRWS
>Mature_364_residues
PTADTDVSAIPETMHAAVYRGSSVVNVEEIPTPSIGPGEILIRVEACGICHTDLKKIEYNLLEPPRIYGHETAGVVAAVG
SGVTRFQIGDRVVAFHHIPCGKCFYCQRKIYAQCPIYKKVGITAGFEPAGGGFAQYVRVMDWIVEEGVERIPDDVSFELA
TLVEPLNTCLKAVVQCDPQPDDFVVVLGQGPIGLMFTMLTRRTGARVAATDTIDTRLELAARCGAEFAWDPRTIDVAAEV
RNLTGGRGADLVIVAASVPGIVNQAIACSRPGSRILLFAQTSATERVETSGADICVGERTLFGCYSASIDLQKESARLVF
GGEIPVEALISHRLPLVKIRSGFDLALHPGPKSLKIIVQPQRWS

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=345, Percent_Identity=25.5072463768116, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI34577061, Length=370, Percent_Identity=26.7567567567568, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI4501933, Length=354, Percent_Identity=25.1412429378531, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI71565154, Length=373, Percent_Identity=24.1286863270777, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1788075, Length=328, Percent_Identity=29.2682926829268, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1788073, Length=338, Percent_Identity=27.2189349112426, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787863, Length=323, Percent_Identity=29.4117647058824, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI87082125, Length=361, Percent_Identity=26.5927977839335, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1790045, Length=345, Percent_Identity=28.1159420289855, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1788407, Length=329, Percent_Identity=27.6595744680851, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI226510992, Length=320, Percent_Identity=26.25, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87081918, Length=267, Percent_Identity=32.5842696629214, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1786825, Length=266, Percent_Identity=31.9548872180451, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1786552, Length=356, Percent_Identity=26.123595505618, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790718, Length=204, Percent_Identity=25.9803921568627, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17562876, Length=322, Percent_Identity=30.1242236024845, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17562878, Length=311, Percent_Identity=29.2604501607717, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71988145, Length=287, Percent_Identity=28.5714285714286, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25146526, Length=382, Percent_Identity=23.5602094240838, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17562584, Length=258, Percent_Identity=28.2945736434109, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71997431, Length=349, Percent_Identity=24.6418338108883, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17562582, Length=135, Percent_Identity=30.3703703703704, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17565904, Length=329, Percent_Identity=24.0121580547112, Blast_Score=65, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6323099, Length=358, Percent_Identity=26.536312849162, Blast_Score=114, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6319955, Length=349, Percent_Identity=26.647564469914, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6322619, Length=349, Percent_Identity=26.647564469914, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319257, Length=404, Percent_Identity=27.970297029703, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6323729, Length=261, Percent_Identity=27.5862068965517, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6319258, Length=395, Percent_Identity=24.5569620253165, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6319621, Length=278, Percent_Identity=27.3381294964029, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6320033, Length=366, Percent_Identity=26.2295081967213, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323961, Length=279, Percent_Identity=26.8817204301075, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6324486, Length=259, Percent_Identity=27.027027027027, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI17137530, Length=363, Percent_Identity=25.8953168044077, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI17737897, Length=363, Percent_Identity=25.068870523416, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17737895, Length=375, Percent_Identity=24.5333333333333, Blast_Score=82, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 39316; Mature: 39185

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTADTDVSAIPETMHAAVYRGSSVVNVEEIPTPSIGPGEILIRVEACGICHTDLKKIEY
CCCCCCCHHHHHHHHHHHHHCCCCEEEHHHCCCCCCCCCEEEEEEEECCCCHHHHHHHHH
NLLEPPRIYGHETAGVVAAVGSGVTRFQIGDRVVAFHHIPCGKCFYCQRKIYAQCPIYKK
CCCCCCCCCCCCCCCHHHHHCCCCEEEEECCEEEEEECCCCCCEEEHHHHHHHCCCCCCC
VGITAGFEPAGGGFAQYVRVMDWIVEEGVERIPDDVSFELATLVEPLNTCLKAVVQCDPQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
PDDFVVVLGQGPIGLMFTMLTRRTGARVAATDTIDTRLELAARCGAEFAWDPRTIDVAAE
CCCEEEEECCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCEEEHHHH
VRNLTGGRGADLVIVAASVPGIVNQAIACSRPGSRILLFAQTSATERVETSGADICVGER
HHHCCCCCCCCEEEEEECCCCHHHHHHHCCCCCCEEEEEEECCCCHHHHCCCCCEEECCC
TLFGCYSASIDLQKESARLVFGGEIPVEALISHRLPLVKIRSGFDLALHPGPKSLKIIVQ
EEEEEEECEEEEECCCCEEEECCCCCHHHHHHCCCCEEEEECCCEEEECCCCCCEEEEEE
PQRWS
CCCCC
>Mature Secondary Structure 
PTADTDVSAIPETMHAAVYRGSSVVNVEEIPTPSIGPGEILIRVEACGICHTDLKKIEY
CCCCCCHHHHHHHHHHHHHCCCCEEEHHHCCCCCCCCCEEEEEEEECCCCHHHHHHHHH
NLLEPPRIYGHETAGVVAAVGSGVTRFQIGDRVVAFHHIPCGKCFYCQRKIYAQCPIYKK
CCCCCCCCCCCCCCCHHHHHCCCCEEEEECCEEEEEECCCCCCEEEHHHHHHHCCCCCCC
VGITAGFEPAGGGFAQYVRVMDWIVEEGVERIPDDVSFELATLVEPLNTCLKAVVQCDPQ
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
PDDFVVVLGQGPIGLMFTMLTRRTGARVAATDTIDTRLELAARCGAEFAWDPRTIDVAAE
CCCEEEEECCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCEEEHHHH
VRNLTGGRGADLVIVAASVPGIVNQAIACSRPGSRILLFAQTSATERVETSGADICVGER
HHHCCCCCCCCEEEEEECCCCHHHHHHHCCCCCCEEEEEEECCCCHHHHCCCCCEEECCC
TLFGCYSASIDLQKESARLVFGGEIPVEALISHRLPLVKIRSGFDLALHPGPKSLKIIVQ
EEEEEEECEEEEECCCCEEEECCCCCHHHHHHCCCCEEEEECCCEEEECCCCCCEEEEEE
PQRWS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1460002; 9384377; 8195086 [H]