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The map label for this gene is ogt [H]

Identifier: 116620381

GI number: 116620381

Start: 1556802

End: 1557287

Strand: Reverse

Name: ogt [H]

Synonym: Acid_1258

Alternate gene names: 116620381

Gene position: 1557287-1556802 (Counterclockwise)

Preceding gene: 116620390

Following gene: 116620376

Centisome position: 15.63

GC content: 61.32

Gene sequence:

>486_bases
ATGATCGAGTGGACCGAAATCTGCTTGACAGATGATTTGACCGTCCGTCTGGTTACAAGTAAACTGGGCCTCCGCGCTAT
AAATTTTCGTCTTTCGGAGCCGGTGGAAGGCGAACGCAACGACTCCAACCCCATCGCCCGCGAAACCGCCCGGCAGCTCG
CTGCCTATTTCGACGGCCGCCTGCGAAGCTTCGATCTGGCCCTCGACCTTCAGGGTACCGCCTTCCAAACCCGTGTTTGG
CGCGAACTGGAACGCATTCCGTACGGGAAAACCCGAAGCTACCTGCAGATCGCCGAAGCCATCGCCTCCCCGAAAGCCGT
TCGGGCCGTCGGGGCGGCCAATGGCGCCAACCCGATCCCGATCGTGATCCCCTGCCATCGCGTAATCGGCTCTACTGGCA
GACTCGTTGGCTACGGCGGCGGCCTCCCGCTGAAAAAGCGGCTGCTCACCCTGGAAGGCGCCCTGGCTTTAGACTTGCGC
TGGTAG

Upstream 100 bases:

>100_bases
CCGAAATTTGGTGCGAAAGGCTTAAGAGGCATAGAAGTGATTGAGATCGATTATAACAGGTGGAAGACAAACGCCGCAGG
TCGTATGACAAACTGGGGGC

Downstream 100 bases:

>100_bases
CACGCATCTGGCTGGTAGCCCGCATCCGGCGGATCTGTTGCAGTGCGGCCTCAAACGAAACTCCAGGCGTTCTCCTGGTA
CCATCTCCCACAGAAATCGC

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MIEWTEICLTDDLTVRLVTSKLGLRAINFRLSEPVEGERNDSNPIARETARQLAAYFDGRLRSFDLALDLQGTAFQTRVW
RELERIPYGKTRSYLQIAEAIASPKAVRAVGAANGANPIPIVIPCHRVIGSTGRLVGYGGGLPLKKRLLTLEGALALDLR
W

Sequences:

>Translated_161_residues
MIEWTEICLTDDLTVRLVTSKLGLRAINFRLSEPVEGERNDSNPIARETARQLAAYFDGRLRSFDLALDLQGTAFQTRVW
RELERIPYGKTRSYLQIAEAIASPKAVRAVGAANGANPIPIVIPCHRVIGSTGRLVGYGGGLPLKKRLLTLEGALALDLR
W
>Mature_161_residues
MIEWTEICLTDDLTVRLVTSKLGLRAINFRLSEPVEGERNDSNPIARETARQLAAYFDGRLRSFDLALDLQGTAFQTRVW
RELERIPYGKTRSYLQIAEAIASPKAVRAVGAANGANPIPIVIPCHRVIGSTGRLVGYGGGLPLKKRLLTLEGALALDLR
W

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=85, Percent_Identity=45.8823529411765, Blast_Score=77, Evalue=8e-15,
Organism=Escherichia coli, GI1787596, Length=107, Percent_Identity=51.4018691588785, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI1788542, Length=107, Percent_Identity=46.7289719626168, Blast_Score=98, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI115533068, Length=89, Percent_Identity=50.561797752809, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=82, Percent_Identity=52.4390243902439, Blast_Score=88, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320001, Length=90, Percent_Identity=42.2222222222222, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17800; Mature: 17800

Theoretical pI: Translated: 9.89; Mature: 9.89

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEWTEICLTDDLTVRLVTSKLGLRAINFRLSEPVEGERNDSNPIARETARQLAAYFDGR
CCCCEEEEECCCCEEEEEEHHHCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCC
LRSFDLALDLQGTAFQTRVWRELERIPYGKTRSYLQIAEAIASPKAVRAVGAANGANPIP
CEEEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHECCCCCCCCCE
IVIPCHRVIGSTGRLVGYGGGLPLKKRLLTLEGALALDLRW
EEEECHHHHCCCCCEEEECCCCCHHHHHEEECCEEEEEECC
>Mature Secondary Structure
MIEWTEICLTDDLTVRLVTSKLGLRAINFRLSEPVEGERNDSNPIARETARQLAAYFDGR
CCCCEEEEECCCCEEEEEEHHHCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCC
LRSFDLALDLQGTAFQTRVWRELERIPYGKTRSYLQIAEAIASPKAVRAVGAANGANPIP
CEEEEEEEECCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHECCCCCCCCCE
IVIPCHRVIGSTGRLVGYGGGLPLKKRLLTLEGALALDLRW
EEEECHHHHCCCCCEEEECCCCCHHHHHEEECCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA