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The map label for this gene is nifL [H]

Identifier: 116620390

GI number: 116620390

Start: 1566167

End: 1567321

Strand: Reverse

Name: nifL [H]

Synonym: Acid_1267

Alternate gene names: 116620390

Gene position: 1567321-1566167 (Counterclockwise)

Preceding gene: 116620391

Following gene: 116620381

Centisome position: 15.73

GC content: 60.87

Gene sequence:

>1155_bases
GTGCAAGAGCCCGGACTGGAAATATCGACGTCGCCCAACGACGACTCGTTGTCGGTGGATATACAGCGCATGAGCCTGGA
GAGCGCCATTCGTCAGGCCGCCGAAGCCATCGTCATCACCAGCGCTACCGGCAAGATTCAATATGTCAATCCGGCCTTCA
CGCGAATGACGGGGTACCGGTCCGATGAGGTGCTCGGCCATTACCCCAACGTGTTGAAATCCGGCACGCAAGACCCTGTC
TATTACGAGCAACTCTGGCAGACGATCCGCGCCGGGCGCGTCTGGCGCGGGGAACTCATCAACCGCCGCAAGGACGGTAG
CTTTTACACCGAAGAAATGTCCATCACGCCGGTCCTCAATGCCAGCGGAAGGATTACCAGCTACATCGCGATCAAGCAGG
ATGTGACCGAGCGCAGGGCAGCCGAAGCGGCCCGGCAATTCCTGGCCGCCATTGTCGCCTCCACCGAAGATGCAGTCGTG
GGCAGCACACTCGATGGCAAGATTCTCGCCTGGAATCACGGTGCGGAAAAGCTGCACGGGTATGCGGCCGAGGAGGTTCT
GGGGCAGCCGGTCGAGATTCTGCTGGCCCCGGAACATCACGATCAATGCCGGCGGATCGCTCAGGGCCTGCTTCGCGGTC
GGACTTTTCCGCGGTTTGACTGCACGGCGATGCGCAAAAGCGGCGAGCGGGTTCAGGTCTCACTATCGGTTTCGCTTATC
AGGAACTGGGCAGGCCAGCCCACCGGTTCGGCCGCCATCATTCGGGACGTCACCGCCGCCCGGCGAATCGCCAGGGAACT
CCGCAATAACGAGGCCCGCTTCCGGACCGCGTTTGAGCGCGCGCCCTTCGGGATGTGCATCACCACCGCGGATTCGCACA
TCCTGCGGGTCAACGAAACCTTCTGCCGCATGCTGGGCTACTCCGCGGAAGAACTGCTCGAACTCGGCTGGTGGGATATT
ACGCACCCGGAGGACCAGGAGCAATGCCTGCGGATTTACCAGCAAATGCTGTTGGAGCACGAGAGCAGCGTCGAATTCCT
CAAACGCTACATTCACAAGCAGGGCCACACAGTCTGCGCGCGCGTCAGCATGTCGGCGATCGATCTGGATGGCTTGTGCG
AATTTCTCGCCCACGTTCAGGAAGTCGATTCCTGA

Upstream 100 bases:

>100_bases
AACGATCCCGATCCCCGTCAATTGAGCCTGCTCGCCGACTGACCTACCAACCTACAGTTTCACCCCACATTTGCCGATAT
GGGTTCAGGAACTGCAAAAA

Downstream 100 bases:

>100_bases
CTGCGGCTCCGCTGCCCCGTGGGGCGTCCACCCAGAGACGTGGTCTGCGGGTAGCGCTCCCTCACGGTCGCGGCTCGTTG
GCGCGGTTGTTAGCTCACCA

Product: putative PAS/PAC sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MQEPGLEISTSPNDDSLSVDIQRMSLESAIRQAAEAIVITSATGKIQYVNPAFTRMTGYRSDEVLGHYPNVLKSGTQDPV
YYEQLWQTIRAGRVWRGELINRRKDGSFYTEEMSITPVLNASGRITSYIAIKQDVTERRAAEAARQFLAAIVASTEDAVV
GSTLDGKILAWNHGAEKLHGYAAEEVLGQPVEILLAPEHHDQCRRIAQGLLRGRTFPRFDCTAMRKSGERVQVSLSVSLI
RNWAGQPTGSAAIIRDVTAARRIARELRNNEARFRTAFERAPFGMCITTADSHILRVNETFCRMLGYSAEELLELGWWDI
THPEDQEQCLRIYQQMLLEHESSVEFLKRYIHKQGHTVCARVSMSAIDLDGLCEFLAHVQEVDS

Sequences:

>Translated_384_residues
MQEPGLEISTSPNDDSLSVDIQRMSLESAIRQAAEAIVITSATGKIQYVNPAFTRMTGYRSDEVLGHYPNVLKSGTQDPV
YYEQLWQTIRAGRVWRGELINRRKDGSFYTEEMSITPVLNASGRITSYIAIKQDVTERRAAEAARQFLAAIVASTEDAVV
GSTLDGKILAWNHGAEKLHGYAAEEVLGQPVEILLAPEHHDQCRRIAQGLLRGRTFPRFDCTAMRKSGERVQVSLSVSLI
RNWAGQPTGSAAIIRDVTAARRIARELRNNEARFRTAFERAPFGMCITTADSHILRVNETFCRMLGYSAEELLELGWWDI
THPEDQEQCLRIYQQMLLEHESSVEFLKRYIHKQGHTVCARVSMSAIDLDGLCEFLAHVQEVDS
>Mature_384_residues
MQEPGLEISTSPNDDSLSVDIQRMSLESAIRQAAEAIVITSATGKIQYVNPAFTRMTGYRSDEVLGHYPNVLKSGTQDPV
YYEQLWQTIRAGRVWRGELINRRKDGSFYTEEMSITPVLNASGRITSYIAIKQDVTERRAAEAARQFLAAIVASTEDAVV
GSTLDGKILAWNHGAEKLHGYAAEEVLGQPVEILLAPEHHDQCRRIAQGLLRGRTFPRFDCTAMRKSGERVQVSLSVSLI
RNWAGQPTGSAAIIRDVTAARRIARELRNNEARFRTAFERAPFGMCITTADSHILRVNETFCRMLGYSAEELLELGWWDI
THPEDQEQCLRIYQQMLLEHESSVEFLKRYIHKQGHTVCARVSMSAIDLDGLCEFLAHVQEVDS

Specific function: Required for the inhibition of nifA activity in response to oxygen and low level of fixed nitrogen [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Homo sapiens, GI71164873, Length=110, Percent_Identity=36.3636363636364, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI71164869, Length=110, Percent_Identity=36.3636363636364, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI26006851, Length=110, Percent_Identity=36.3636363636364, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1788381, Length=136, Percent_Identity=29.4117647058824, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR014285
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767 [H]

Pfam domain/function: PF00989 PAS [H]

EC number: NA

Molecular weight: Translated: 43186; Mature: 43186

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50112 PAS ; PS50113 PAC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEPGLEISTSPNDDSLSVDIQRMSLESAIRQAAEAIVITSATGKIQYVNPAFTRMTGYR
CCCCCCEEECCCCCCCEEEEHHHHHHHHHHHHHHHEEEEEECCCEEEEECHHHHHHCCCC
SDEVLGHYPNVLKSGTQDPVYYEQLWQTIRAGRVWRGELINRRKDGSFYTEEMSITPVLN
CCCHHHCCCHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEECEEEEEEEC
ASGRITSYIAIKQDVTERRAAEAARQFLAAIVASTEDAVVGSTLDGKILAWNHGAEKLHG
CCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEEEECCCHHHHHH
YAAEEVLGQPVEILLAPEHHDQCRRIAQGLLRGRTFPRFDCTAMRKSGERVQVSLSVSLI
HHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEEEEEHHHH
RNWAGQPTGSAAIIRDVTAARRIARELRNNEARFRTAFERAPFGMCITTADSHILRVNET
HHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCEEEEHHH
FCRMLGYSAEELLELGWWDITHPEDQEQCLRIYQQMLLEHESSVEFLKRYIHKQGHTVCA
HHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEE
RVSMSAIDLDGLCEFLAHVQEVDS
EHHHHHCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQEPGLEISTSPNDDSLSVDIQRMSLESAIRQAAEAIVITSATGKIQYVNPAFTRMTGYR
CCCCCCEEECCCCCCCEEEEHHHHHHHHHHHHHHHEEEEEECCCEEEEECHHHHHHCCCC
SDEVLGHYPNVLKSGTQDPVYYEQLWQTIRAGRVWRGELINRRKDGSFYTEEMSITPVLN
CCCHHHCCCHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCEEEECEEEEEEEC
ASGRITSYIAIKQDVTERRAAEAARQFLAAIVASTEDAVVGSTLDGKILAWNHGAEKLHG
CCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCEEEEECCCHHHHHH
YAAEEVLGQPVEILLAPEHHDQCRRIAQGLLRGRTFPRFDCTAMRKSGERVQVSLSVSLI
HHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEEEEEHHHH
RNWAGQPTGSAAIIRDVTAARRIARELRNNEARFRTAFERAPFGMCITTADSHILRVNET
HHCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCEEEEHHH
FCRMLGYSAEELLELGWWDITHPEDQEQCLRIYQQMLLEHESSVEFLKRYIHKQGHTVCA
HHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEE
RVSMSAIDLDGLCEFLAHVQEVDS
EHHHHHCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3027503; 2838726; 1879702; 8481091 [H]