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The map label for this gene is otsA [H]

Identifier: 116620279

GI number: 116620279

Start: 1423791

End: 1426040

Strand: Reverse

Name: otsA [H]

Synonym: Acid_1156

Alternate gene names: 116620279

Gene position: 1426040-1423791 (Counterclockwise)

Preceding gene: 116620281

Following gene: 116620278

Centisome position: 14.31

GC content: 63.73

Gene sequence:

>2250_bases
ATGCGACGGATGGTTCCATTCACTTTGGCCCTTTTAGCGGGTCTTGCCTTACTGGCATGGGCCGCTTCCGGGGTCGTGCA
GACTACCGCTCGTCGGTGGTTTGAGCGCGACATCGGCGCGCGTGCGAACCTTGTCCTGGTCAGCTCCGAAAAGTCGCTCG
CAAATAACTGGAACGGCGAGCCCGGCGAACTTCAAAAACAGCTTGCCGCACTCACTCGCGACGAGCGCGTGATGGGCGCG
GCGGCCTGCGATCCCGATCTCAAGACGCGCGCCGCCACCGTCGGATTTCCCGAGGATTTCGGCTGCTGGGCCGTCGGTAC
CCGGGTCCGTTCGATCGATTCCAACGGCGTCGCCCACTTCCGGCCCTGGAGCACCGTGTCCGACCTGCCCACCGGCCGTG
TGCACGTGAGCGTAATGCCGCTGGCCAATCCGGCGGGTCAGGAACTCGGCTTCGCGATCCTCCTCCACGACCTCAGCTTC
ATCGATCGCCGCGAGGCGCAGGCGCGTACGTTTCTCTTCGCCACCTTTGGGATTCTTGCCTTCATCGCGCTTTTGCTCCC
CGTGTGGGTCGCCAGACTCGCGCGCTACGATTGGAGCCGCGAGTTGAGCAGCCTGCTCCGCGGCGGCGGCAAGCAGCAGC
AGAGCCGCGAGTTCCAACCGATCCTCAGCGATGTTCGCGAGTTGGTGAGCCGCATGGCATACGATCGCGACGACGCTCCC
GGCCAGTGGAATGCCGAGCGGCTGAAGCACAGCCTGAATAGCAATCTGCACGGCGAGAGGGTCGTCATTCTCTCCAACCG
CGAGCCCTATATTCACGAATTCACCCCCCATGGAGGTATCGAGGTGCAGCATCCCGCCAGCGGCCTGGTCACCGCGCTCG
AACCGGTCATGCGCGCGTGCTCCGGAGTCTGGGTCGCGCACGGCAGCGGCAGCGCCGATCGCGAAACCGTGGATCGTAAG
GATCACGTGCGCGTCCCTCCCGGTGAAGAGTCGTATCACATTCGCCGCGTCTGGCTCACCGAGGAAGAGCAGCAGGGCTA
CTACTACGGCTTCTCCAACGAAGGCCTCTGGCCTCTCTGCCACGTCGCCTATGCACGCCCCATCTTCCGCGCCGAGGACT
GGCGCCAGTACCGCGCCGTCAACCAGAAGTTCGCCGACGCCGTCTTGAGCGAAGTCGATTCGAGCGATCCCATTATTCTC
GTGCAGGATTACCACTTCGCACTCGTGCCGGCCATGATCCGCAAGCGCCTGCCCGCCGCCACCGTCATCATGTTCTGGCA
CATCCCCTGGCCCAACGCGGAAAATGTCGGCATCTGTCCCTGGCGCAATCAATTGCTCGAAGGCCTCCTGGGCAGCAGCA
TCCTTGGCTTCCACACCCAGCTGCATTGCAATAACTTCCTGGATTCGGTGGATCGTTACCTGGAAACACGCATCGACCGC
GAGAACAATGCCGTAGTGATGCAGGGGCATCGCACCCTTATACGGCCCTATCCGATTTCGCTCGAATGGCCCGTCCACTG
GGCAGTTGAGGCGCCCAGCCCCGAGGATTGCCGCGCTCAGGTCTGGCGTGACCTCGGCTTGAAACGCGACGCGCTCCTCG
GAGTCGGTATCGATCGCCTCGATTACACCAAAGGCATTGAGGAGCGCCTGCTCGCCGTGGAGCAACTCCTGGAGCGCTAC
CCGGAATACCGTGGCCGCTTCAGCTTCGCCCAGCTCGCCGCTCCCAGCCGCACCAAAATCGAGCGCTACCGCCAACTCAG
CGAGGACGTGGAGCGCCTGGCCGAGCGCATCAATCAGCGCTTCGGAAAACCAGATTACCGCCCCGTGATTCTGCTCCGCT
CCCACCACGAGCCGCCCGAGGTCTTTCGCTACTTCCGCGCCTCCGATCTCTGCTACGTGAGTTCCCTGCACGACGGCATG
AACCTCGTGGCCAAGGAATATGTCGCCGCACGCACCGACCTGAAAGGCGTGCTGGTGCTCAGCGAATTCACCGGCGCGGC
GCGAGAATTGAGCGAAGCCCTTATCGTCAACCCCTACGACCTGGAGGCCAGCAGCGATGCGCTCGCCGCCGCGCTCGCCA
TGCCGCTCGAAGAGCAGCGCGACCGCATGGCATCGATGCGCTCGCTTCTCGTACAGTTCAACGTGTATCGCTGGGCGGGA
AGGATGCTGGTGGACGCGGCGCGCCTGCGCAACCAGGAGCGAGTCGCCGGACGTCTGGCGGAGCGAACCGGCGTCGGTTC
ATCGGCCTGA

Upstream 100 bases:

>100_bases
TCAGGGCCGAAAACTGACAGTTTCCGATGACTCCGAGGTCTTCGAGCTGCATATTTCGCACAGTAGCATAGACCCCGGAT
GCTTTAATGAAAGTAGAAGA

Downstream 100 bases:

>100_bases
GCATGGAATACGCTCTCTCCCGCTCCGCGCGTCAGTTGCTGGCGCAGCTTTCCTGCGAACGCACGCTCTATGCCTTCGAT
TTCGATGGCACGCTCTCGCC

Product: alpha,alpha-trehalose-phosphate synthase

Products: UDP; alpha,alpha'-Trehalose 6-phosphate

Alternate protein names: TPS; Trehalose-6-phosphate synthase [H]

Number of amino acids: Translated: 749; Mature: 749

Protein sequence:

>749_residues
MRRMVPFTLALLAGLALLAWAASGVVQTTARRWFERDIGARANLVLVSSEKSLANNWNGEPGELQKQLAALTRDERVMGA
AACDPDLKTRAATVGFPEDFGCWAVGTRVRSIDSNGVAHFRPWSTVSDLPTGRVHVSVMPLANPAGQELGFAILLHDLSF
IDRREAQARTFLFATFGILAFIALLLPVWVARLARYDWSRELSSLLRGGGKQQQSREFQPILSDVRELVSRMAYDRDDAP
GQWNAERLKHSLNSNLHGERVVILSNREPYIHEFTPHGGIEVQHPASGLVTALEPVMRACSGVWVAHGSGSADRETVDRK
DHVRVPPGEESYHIRRVWLTEEEQQGYYYGFSNEGLWPLCHVAYARPIFRAEDWRQYRAVNQKFADAVLSEVDSSDPIIL
VQDYHFALVPAMIRKRLPAATVIMFWHIPWPNAENVGICPWRNQLLEGLLGSSILGFHTQLHCNNFLDSVDRYLETRIDR
ENNAVVMQGHRTLIRPYPISLEWPVHWAVEAPSPEDCRAQVWRDLGLKRDALLGVGIDRLDYTKGIEERLLAVEQLLERY
PEYRGRFSFAQLAAPSRTKIERYRQLSEDVERLAERINQRFGKPDYRPVILLRSHHEPPEVFRYFRASDLCYVSSLHDGM
NLVAKEYVAARTDLKGVLVLSEFTGAARELSEALIVNPYDLEASSDALAAALAMPLEEQRDRMASMRSLLVQFNVYRWAG
RMLVDAARLRNQERVAGRLAERTGVGSSA

Sequences:

>Translated_749_residues
MRRMVPFTLALLAGLALLAWAASGVVQTTARRWFERDIGARANLVLVSSEKSLANNWNGEPGELQKQLAALTRDERVMGA
AACDPDLKTRAATVGFPEDFGCWAVGTRVRSIDSNGVAHFRPWSTVSDLPTGRVHVSVMPLANPAGQELGFAILLHDLSF
IDRREAQARTFLFATFGILAFIALLLPVWVARLARYDWSRELSSLLRGGGKQQQSREFQPILSDVRELVSRMAYDRDDAP
GQWNAERLKHSLNSNLHGERVVILSNREPYIHEFTPHGGIEVQHPASGLVTALEPVMRACSGVWVAHGSGSADRETVDRK
DHVRVPPGEESYHIRRVWLTEEEQQGYYYGFSNEGLWPLCHVAYARPIFRAEDWRQYRAVNQKFADAVLSEVDSSDPIIL
VQDYHFALVPAMIRKRLPAATVIMFWHIPWPNAENVGICPWRNQLLEGLLGSSILGFHTQLHCNNFLDSVDRYLETRIDR
ENNAVVMQGHRTLIRPYPISLEWPVHWAVEAPSPEDCRAQVWRDLGLKRDALLGVGIDRLDYTKGIEERLLAVEQLLERY
PEYRGRFSFAQLAAPSRTKIERYRQLSEDVERLAERINQRFGKPDYRPVILLRSHHEPPEVFRYFRASDLCYVSSLHDGM
NLVAKEYVAARTDLKGVLVLSEFTGAARELSEALIVNPYDLEASSDALAAALAMPLEEQRDRMASMRSLLVQFNVYRWAG
RMLVDAARLRNQERVAGRLAERTGVGSSA
>Mature_749_residues
MRRMVPFTLALLAGLALLAWAASGVVQTTARRWFERDIGARANLVLVSSEKSLANNWNGEPGELQKQLAALTRDERVMGA
AACDPDLKTRAATVGFPEDFGCWAVGTRVRSIDSNGVAHFRPWSTVSDLPTGRVHVSVMPLANPAGQELGFAILLHDLSF
IDRREAQARTFLFATFGILAFIALLLPVWVARLARYDWSRELSSLLRGGGKQQQSREFQPILSDVRELVSRMAYDRDDAP
GQWNAERLKHSLNSNLHGERVVILSNREPYIHEFTPHGGIEVQHPASGLVTALEPVMRACSGVWVAHGSGSADRETVDRK
DHVRVPPGEESYHIRRVWLTEEEQQGYYYGFSNEGLWPLCHVAYARPIFRAEDWRQYRAVNQKFADAVLSEVDSSDPIIL
VQDYHFALVPAMIRKRLPAATVIMFWHIPWPNAENVGICPWRNQLLEGLLGSSILGFHTQLHCNNFLDSVDRYLETRIDR
ENNAVVMQGHRTLIRPYPISLEWPVHWAVEAPSPEDCRAQVWRDLGLKRDALLGVGIDRLDYTKGIEERLLAVEQLLERY
PEYRGRFSFAQLAAPSRTKIERYRQLSEDVERLAERINQRFGKPDYRPVILLRSHHEPPEVFRYFRASDLCYVSSLHDGM
NLVAKEYVAARTDLKGVLVLSEFTGAARELSEALIVNPYDLEASSDALAAALAMPLEEQRDRMASMRSLLVQFNVYRWAG
RMLVDAARLRNQERVAGRLAERTGVGSSA

Specific function: Catalyzes the transfer of glucose from a nucleoside diphosphate-glucose to glucose-6-phosphate to form trehalose-6- phosphate and nucleoside diphosphate [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=473, Percent_Identity=34.2494714587738, Blast_Score=217, Evalue=2e-57,
Organism=Caenorhabditis elegans, GI115533560, Length=488, Percent_Identity=25.6147540983607, Blast_Score=125, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI115533558, Length=488, Percent_Identity=25.6147540983607, Blast_Score=125, Evalue=8e-29,
Organism=Caenorhabditis elegans, GI71987012, Length=433, Percent_Identity=27.0207852193995, Blast_Score=121, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6319602, Length=445, Percent_Identity=35.2808988764045, Blast_Score=256, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6323537, Length=408, Percent_Identity=28.921568627451, Blast_Score=166, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6323917, Length=400, Percent_Identity=27.5, Blast_Score=159, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6320279, Length=440, Percent_Identity=26.8181818181818, Blast_Score=150, Evalue=5e-37,
Organism=Drosophila melanogaster, GI19920676, Length=482, Percent_Identity=34.8547717842324, Blast_Score=270, Evalue=3e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: 2.4.1.15

Molecular weight: Translated: 84745; Mature: 84745

Theoretical pI: Translated: 7.21; Mature: 7.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRMVPFTLALLAGLALLAWAASGVVQTTARRWFERDIGARANLVLVSSEKSLANNWNGE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHCCCCC
PGELQKQLAALTRDERVMGAAACDPDLKTRAATVGFPEDFGCWAVGTRVRSIDSNGVAHF
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHCEEECCCCCCCCCHHHCCCCEECCCCCCEEE
RPWSTVSDLPTGRVHVSVMPLANPAGQELGFAILLHDLSFIDRREAQARTFLFATFGILA
CCCCCHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIALLLPVWVARLARYDWSRELSSLLRGGGKQQQSREFQPILSDVRELVSRMAYDRDDAP
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GQWNAERLKHSLNSNLHGERVVILSNREPYIHEFTPHGGIEVQHPASGLVTALEPVMRAC
CCCCHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCCCCEEECCCCHHHHHHHHHHHHHH
SGVWVAHGSGSADRETVDRKDHVRVPPGEESYHIRRVWLTEEEQQGYYYGFSNEGLWPLC
CCEEEECCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCHHHH
HVAYARPIFRAEDWRQYRAVNQKFADAVLSEVDSSDPIILVQDYHFALVPAMIRKRLPAA
HHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCE
TVIMFWHIPWPNAENVGICPWRNQLLEGLLGSSILGFHTQLHCNNFLDSVDRYLETRIDR
EEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ENNAVVMQGHRTLIRPYPISLEWPVHWAVEAPSPEDCRAQVWRDLGLKRDALLGVGIDRL
CCCEEEEECCCEEECCCCCEEECCEEEEEECCCCHHHHHHHHHHCCCCCHHHHCCCCHHH
DYTKGIEERLLAVEQLLERYPEYRGRFSFAQLAAPSRTKIERYRQLSEDVERLAERINQR
HHHCCHHHHHHHHHHHHHHCHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FGKPDYRPVILLRSHHEPPEVFRYFRASDLCYVSSLHDGMNLVAKEYVAARTDLKGVLVL
CCCCCCCEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
SEFTGAARELSEALIVNPYDLEASSDALAAALAMPLEEQRDRMASMRSLLVQFNVYRWAG
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RMLVDAARLRNQERVAGRLAERTGVGSSA
HHHHHHHHHCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRRMVPFTLALLAGLALLAWAASGVVQTTARRWFERDIGARANLVLVSSEKSLANNWNGE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHCCCCC
PGELQKQLAALTRDERVMGAAACDPDLKTRAATVGFPEDFGCWAVGTRVRSIDSNGVAHF
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHCEEECCCCCCCCCHHHCCCCEECCCCCCEEE
RPWSTVSDLPTGRVHVSVMPLANPAGQELGFAILLHDLSFIDRREAQARTFLFATFGILA
CCCCCHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIALLLPVWVARLARYDWSRELSSLLRGGGKQQQSREFQPILSDVRELVSRMAYDRDDAP
HHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GQWNAERLKHSLNSNLHGERVVILSNREPYIHEFTPHGGIEVQHPASGLVTALEPVMRAC
CCCCHHHHHHHHCCCCCCCEEEEEECCCCCEEECCCCCCEEECCCCHHHHHHHHHHHHHH
SGVWVAHGSGSADRETVDRKDHVRVPPGEESYHIRRVWLTEEEQQGYYYGFSNEGLWPLC
CCEEEECCCCCCCHHHCCCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEECCCCCHHHH
HVAYARPIFRAEDWRQYRAVNQKFADAVLSEVDSSDPIILVQDYHFALVPAMIRKRLPAA
HHHHHCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCE
TVIMFWHIPWPNAENVGICPWRNQLLEGLLGSSILGFHTQLHCNNFLDSVDRYLETRIDR
EEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ENNAVVMQGHRTLIRPYPISLEWPVHWAVEAPSPEDCRAQVWRDLGLKRDALLGVGIDRL
CCCEEEEECCCEEECCCCCEEECCEEEEEECCCCHHHHHHHHHHCCCCCHHHHCCCCHHH
DYTKGIEERLLAVEQLLERYPEYRGRFSFAQLAAPSRTKIERYRQLSEDVERLAERINQR
HHHCCHHHHHHHHHHHHHHCHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
FGKPDYRPVILLRSHHEPPEVFRYFRASDLCYVSSLHDGMNLVAKEYVAARTDLKGVLVL
CCCCCCCEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEE
SEFTGAARELSEALIVNPYDLEASSDALAAALAMPLEEQRDRMASMRSLLVQFNVYRWAG
HHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RMLVDAARLRNQERVAGRLAERTGVGSSA
HHHHHHHHHCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Monovalent cations [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-glucose; D-glucose 6-phosphate

Specific reaction: UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate

General reaction: Hexosyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA