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The map label for this gene is otsB [H]

Identifier: 116620278

GI number: 116620278

Start: 1422991

End: 1423788

Strand: Reverse

Name: otsB [H]

Synonym: Acid_1155

Alternate gene names: 116620278

Gene position: 1423788-1422991 (Counterclockwise)

Preceding gene: 116620279

Following gene: 116620274

Centisome position: 14.29

GC content: 67.17

Gene sequence:

>798_bases
ATGGAATACGCTCTCTCCCGCTCCGCGCGTCAGTTGCTGGCGCAGCTTTCCTGCGAACGCACGCTCTATGCCTTCGATTT
CGATGGCACGCTCTCGCCCATCGTGTCCAATCCCGACCACGCCGTCATGAGTCGCCGGACCCGTGGCCTGCTCACCTGCC
TGGCCGCCGCGCGCCCTTGCATCGTCGTCTCCGGGCGCGGCCGCGCGGACCTCATGCAGCGTGTGAGTGGTGTTGGCCTG
GCGCAGGCCATCGGCAATCATGGCGCCGAAACCAGCGAAGCCTCTCGCGCGCCGCGCCGCCGTATCGCGCAATGGAAGGC
CGCCCTCGAACCCGCGCTTCGCGATTTACCCGGCGTATGGGTAGAGGATAAGGATCTCTCCCTCGCCGTTCACTATCGCC
AGGCTCCCAAGAGCTCAGATGCCCGCTGCCGCATTCTCGCCGCCGCGCGAACCCTGCCGCACGCCCGCGTCTTCGGCGGC
AAGCGCGTGGTCAATCTCGTGCTCGATACCGCCCCCCACAAGGGCCAGGCGCTCGCCGCCGAACGCGACCGCCTCGGCTG
CGATTGGGTCCTCTACGTCGGCGACGACAAAACCGATGAGGACGCCTTCGCTCTCTGCGGCAATATCGTCCCTGTCCGCA
TCGGCGTCAGCCAGCGCTCCCACGCCCGCTACTATCTGCGCACGCAACGCGAAATCGACCGCCTGCTCGCCGTCCTGGTG
CGCAAGACCAAGGCGGTGGAGAACGCTCTCGTAGGGCATACGGTTTCGCCCGCCGACCAGCCCCAAGGCCAGCTCTGA

Upstream 100 bases:

>100_bases
GGGCGGGAAGGATGCTGGTGGACGCGGCGCGCCTGCGCAACCAGGAGCGAGTCGCCGGACGTCTGGCGGAGCGAACCGGC
GTCGGTTCATCGGCCTGAGC

Downstream 100 bases:

>100_bases
AGTGTATGGTGTCAGCGGAAATTCTCCACGAGCACCACATCGCTGGCCGGAACGGCGGAATTGGCGGGACTGGCGAAGGC
AATCCAGCGGCGATCGGGAG

Product: HAD family hydrolase

Products: NA

Alternate protein names: TPP; Trehalose-6-phosphate phosphatase [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MEYALSRSARQLLAQLSCERTLYAFDFDGTLSPIVSNPDHAVMSRRTRGLLTCLAAARPCIVVSGRGRADLMQRVSGVGL
AQAIGNHGAETSEASRAPRRRIAQWKAALEPALRDLPGVWVEDKDLSLAVHYRQAPKSSDARCRILAAARTLPHARVFGG
KRVVNLVLDTAPHKGQALAAERDRLGCDWVLYVGDDKTDEDAFALCGNIVPVRIGVSQRSHARYYLRTQREIDRLLAVLV
RKTKAVENALVGHTVSPADQPQGQL

Sequences:

>Translated_265_residues
MEYALSRSARQLLAQLSCERTLYAFDFDGTLSPIVSNPDHAVMSRRTRGLLTCLAAARPCIVVSGRGRADLMQRVSGVGL
AQAIGNHGAETSEASRAPRRRIAQWKAALEPALRDLPGVWVEDKDLSLAVHYRQAPKSSDARCRILAAARTLPHARVFGG
KRVVNLVLDTAPHKGQALAAERDRLGCDWVLYVGDDKTDEDAFALCGNIVPVRIGVSQRSHARYYLRTQREIDRLLAVLV
RKTKAVENALVGHTVSPADQPQGQL
>Mature_265_residues
MEYALSRSARQLLAQLSCERTLYAFDFDGTLSPIVSNPDHAVMSRRTRGLLTCLAAARPCIVVSGRGRADLMQRVSGVGL
AQAIGNHGAETSEASRAPRRRIAQWKAALEPALRDLPGVWVEDKDLSLAVHYRQAPKSSDARCRILAAARTLPHARVFGG
KRVVNLVLDTAPHKGQALAAERDRLGCDWVLYVGDDKTDEDAFALCGNIVPVRIGVSQRSHARYYLRTQREIDRLLAVLV
RKTKAVENALVGHTVSPADQPQGQL

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Escherichia coli, GI1788207, Length=214, Percent_Identity=30.8411214953271, Blast_Score=62, Evalue=3e-11,
Organism=Drosophila melanogaster, GI221473368, Length=196, Percent_Identity=30.1020408163265, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI20129307, Length=196, Percent_Identity=30.1020408163265, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24582490, Length=196, Percent_Identity=30.1020408163265, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 29021; Mature: 29021

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYALSRSARQLLAQLSCERTLYAFDFDGTLSPIVSNPDHAVMSRRTRGLLTCLAAARPC
CCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCE
IVVSGRGRADLMQRVSGVGLAQAIGNHGAETSEASRAPRRRIAQWKAALEPALRDLPGVW
EEEECCCHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VEDKDLSLAVHYRQAPKSSDARCRILAAARTLPHARVFGGKRVVNLVLDTAPHKGQALAA
EECCCCEEEEEEECCCCCCCCEEEEEEHHHHCCHHHHHCCHHHHHHHHHCCCCCCCCHHH
ERDRLGCDWVLYVGDDKTDEDAFALCGNIVPVRIGVSQRSHARYYLRTQREIDRLLAVLV
HHHCCCCCEEEEECCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
RKTKAVENALVGHTVSPADQPQGQL
HHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MEYALSRSARQLLAQLSCERTLYAFDFDGTLSPIVSNPDHAVMSRRTRGLLTCLAAARPC
CCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCE
IVVSGRGRADLMQRVSGVGLAQAIGNHGAETSEASRAPRRRIAQWKAALEPALRDLPGVW
EEEECCCHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCE
VEDKDLSLAVHYRQAPKSSDARCRILAAARTLPHARVFGGKRVVNLVLDTAPHKGQALAA
EECCCCEEEEEEECCCCCCCCEEEEEEHHHHCCHHHHHCCHHHHHHHHHCCCCCCCCHHH
ERDRLGCDWVLYVGDDKTDEDAFALCGNIVPVRIGVSQRSHARYYLRTQREIDRLLAVLV
HHHCCCCCEEEEECCCCCCHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHH
RKTKAVENALVGHTVSPADQPQGQL
HHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]