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The map label for this gene is sthA [H]

Identifier: 116620198

GI number: 116620198

Start: 1323959

End: 1325347

Strand: Reverse

Name: sthA [H]

Synonym: Acid_1071

Alternate gene names: 116620198

Gene position: 1325347-1323959 (Counterclockwise)

Preceding gene: 116620199

Following gene: 116620197

Centisome position: 13.3

GC content: 60.62

Gene sequence:

>1389_bases
ATGCAGAAATTCGACATCATCGTCATCGGTTCCGGTCCCGCCGGCCAGCGCGCGGCGATCCAGGGCGCAAAATGCGGCCG
GCGGGTCGCCGTCATTGAGCAGCGCGAAATCGTCGGCGGAGCCTGCGTCAATACCGGAACCATTCCCAGCAAGACGATGC
GTGAAGCCGTGATGCACCTCTCGGGCTTCCAGTACCAGGGCATCTACGGCAGCAACTACCACGTCAAGGAAAAGATCCTG
ATGGCCGACCTCGGCTTCCGGGTCAAACAGGTCATCAAGACCGAGGTGGATGTAACCCAGGCACAGCTGACGCGCAACGG
AGTGGAAGTGATCACGGGCCGCGCGGGTTTCCTCGATTCCACTCACGTGCGCGTGGAAAGCTCTCGCGGACAGACGGACC
TGGAAGCGCCGATCATCATCATCGCCACTGGCACCAAACCCTCCACCAGCCCGACGGTGCCGCTCAATGGCCGCACCATC
GTCAACAGCGATCAGATTCTGGACATTCCGGAAATTCCGAAGACCCTGATCGTGGTGGGCGGCGGCGTGATCGGCGTGGA
ATACACTTGTATCTTCGCGATCCTGGGGGTGCGCGTCATTCTGATCGAAAAGCGCCCTCGGCTGCTCGAATTCGCCGATT
CGGAGATGGTCGAAGCGCTCTCCTATCACCTGCGCGACCATCGCGTGACCATGCGACTCAACGAGGAAGTCGCCGCCGTG
GAAGAGCAGGCAGGCGGCGGGGTGGTGGCACGGCTCAAGAGCAACAAGGTGATCTCCGGCGACGCGCTCTTGTACGCGGT
CGGACGTCAGGGCAATGTGGAGGATTTGAATCTGCCCGCTGCCGGCCTGGAGGCCGATGGCCGCGGCCGTATCACGGTCG
ATTCCGACTATCGCACCAAGACGTCCAACATTTATGCGGCCGGCGACGTGATCGGCTTCCCGAGCCTGGCATCGGTATCG
ATGGAACAGGGGCGCCTCGCGGCCGCCAACGCGGTCGGGCTGAAGATCCAGTCCAATCCGGCAACTTATCCATACGGCAT
CTATACGATCCCCGAGATTTCCTTCGTGGGCAAGACGGAAGAGCAGCTAACGGCCGACGACGTTCCGTACGAGGTCGGAG
TCGCGTACTATCGGGAAACCGCGCGCGGCCAGATCCGCGGCGATACCACCGGCCGCCTGAAGCTGATTTTCCACCGCGAG
ACGCGCGAAATTCTAGGGGTGCACATCATCGGCGAAGGGGCAACCGAATTGCTCCATATCGGCCAGGCGGTGATGATTCT
GAAGGGCACGGTCGATTACTTCGTGGAAACGGTATTCAACTATCCCACGCTGGCGGAATGTTATAAGCAAGCCGCATTCA
ATGGCATCAACAAGCTGATGCGGTTTTAG

Upstream 100 bases:

>100_bases
GTGGAACGTGGTCAACTGGAAGGAAATCGAAGACCGCTTCAACGCCAGGTAATACACGCCTGTTAGAGTAATTGGGCGGG
TCAATCCCCCGCCCTCTCTC

Downstream 100 bases:

>100_bases
GAGGGGGCTCCATGGGGCGCAGCATCGGAGTGCTGGCCACGCGTTATCTTTGGGCCGGAATCGTCGAAGGCGAGCAGTTG
GGAGACGTCAAAGTCTTTCC

Product: soluble pyridine nucleotide transhydrogenase

Products: NA

Alternate protein names: STH; NAD(P)(+) transhydrogenase [B-specific] [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MQKFDIIVIGSGPAGQRAAIQGAKCGRRVAVIEQREIVGGACVNTGTIPSKTMREAVMHLSGFQYQGIYGSNYHVKEKIL
MADLGFRVKQVIKTEVDVTQAQLTRNGVEVITGRAGFLDSTHVRVESSRGQTDLEAPIIIIATGTKPSTSPTVPLNGRTI
VNSDQILDIPEIPKTLIVVGGGVIGVEYTCIFAILGVRVILIEKRPRLLEFADSEMVEALSYHLRDHRVTMRLNEEVAAV
EEQAGGGVVARLKSNKVISGDALLYAVGRQGNVEDLNLPAAGLEADGRGRITVDSDYRTKTSNIYAAGDVIGFPSLASVS
MEQGRLAAANAVGLKIQSNPATYPYGIYTIPEISFVGKTEEQLTADDVPYEVGVAYYRETARGQIRGDTTGRLKLIFHRE
TREILGVHIIGEGATELLHIGQAVMILKGTVDYFVETVFNYPTLAECYKQAAFNGINKLMRF

Sequences:

>Translated_462_residues
MQKFDIIVIGSGPAGQRAAIQGAKCGRRVAVIEQREIVGGACVNTGTIPSKTMREAVMHLSGFQYQGIYGSNYHVKEKIL
MADLGFRVKQVIKTEVDVTQAQLTRNGVEVITGRAGFLDSTHVRVESSRGQTDLEAPIIIIATGTKPSTSPTVPLNGRTI
VNSDQILDIPEIPKTLIVVGGGVIGVEYTCIFAILGVRVILIEKRPRLLEFADSEMVEALSYHLRDHRVTMRLNEEVAAV
EEQAGGGVVARLKSNKVISGDALLYAVGRQGNVEDLNLPAAGLEADGRGRITVDSDYRTKTSNIYAAGDVIGFPSLASVS
MEQGRLAAANAVGLKIQSNPATYPYGIYTIPEISFVGKTEEQLTADDVPYEVGVAYYRETARGQIRGDTTGRLKLIFHRE
TREILGVHIIGEGATELLHIGQAVMILKGTVDYFVETVFNYPTLAECYKQAAFNGINKLMRF
>Mature_462_residues
MQKFDIIVIGSGPAGQRAAIQGAKCGRRVAVIEQREIVGGACVNTGTIPSKTMREAVMHLSGFQYQGIYGSNYHVKEKIL
MADLGFRVKQVIKTEVDVTQAQLTRNGVEVITGRAGFLDSTHVRVESSRGQTDLEAPIIIIATGTKPSTSPTVPLNGRTI
VNSDQILDIPEIPKTLIVVGGGVIGVEYTCIFAILGVRVILIEKRPRLLEFADSEMVEALSYHLRDHRVTMRLNEEVAAV
EEQAGGGVVARLKSNKVISGDALLYAVGRQGNVEDLNLPAAGLEADGRGRITVDSDYRTKTSNIYAAGDVIGFPSLASVS
MEQGRLAAANAVGLKIQSNPATYPYGIYTIPEISFVGKTEEQLTADDVPYEVGVAYYRETARGQIRGDTTGRLKLIFHRE
TREILGVHIIGEGATELLHIGQAVMILKGTVDYFVETVFNYPTLAECYKQAAFNGINKLMRF

Specific function: Conversion of NADPH, generated by peripheral catabolic pathways, to NADH, which can enter the respiratory chain for energy generation [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=461, Percent_Identity=29.2841648590022, Blast_Score=209, Evalue=3e-54,
Organism=Homo sapiens, GI50301238, Length=476, Percent_Identity=29.2016806722689, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI22035672, Length=456, Percent_Identity=27.1929824561404, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI291045266, Length=432, Percent_Identity=23.6111111111111, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI148277065, Length=457, Percent_Identity=22.9759299781182, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI33519430, Length=457, Percent_Identity=22.9759299781182, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI33519428, Length=457, Percent_Identity=22.9759299781182, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI33519426, Length=457, Percent_Identity=22.9759299781182, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI148277071, Length=457, Percent_Identity=22.9759299781182, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI291045268, Length=424, Percent_Identity=22.6415094339623, Blast_Score=86, Evalue=9e-17,
Organism=Escherichia coli, GI87082354, Length=462, Percent_Identity=46.1038961038961, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1786307, Length=470, Percent_Identity=30, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI87081717, Length=452, Percent_Identity=28.7610619469027, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1789915, Length=446, Percent_Identity=29.8206278026906, Blast_Score=139, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI32565766, Length=460, Percent_Identity=28.9130434782609, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17557007, Length=479, Percent_Identity=26.7223382045929, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71983429, Length=471, Percent_Identity=27.3885350318471, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71983419, Length=471, Percent_Identity=27.3885350318471, Blast_Score=95, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI71982272, Length=490, Percent_Identity=23.469387755102, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6321091, Length=472, Percent_Identity=30.0847457627119, Blast_Score=190, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6325240, Length=466, Percent_Identity=30.0429184549356, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6325166, Length=462, Percent_Identity=24.8917748917749, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21358499, Length=461, Percent_Identity=31.0195227765727, Blast_Score=207, Evalue=8e-54,
Organism=Drosophila melanogaster, GI24640549, Length=479, Percent_Identity=30.6889352818372, Blast_Score=156, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24640553, Length=476, Percent_Identity=30.4621848739496, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24640551, Length=479, Percent_Identity=30.6889352818372, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI17737741, Length=485, Percent_Identity=29.4845360824742, Blast_Score=142, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327
- InterPro:   IPR022962 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.6.1.1 [H]

Molecular weight: Translated: 50129; Mature: 50129

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKFDIIVIGSGPAGQRAAIQGAKCGRRVAVIEQREIVGGACVNTGTIPSKTMREAVMHL
CCEEEEEEECCCCCCCCCEECCCCCCCEEEEEEHHHHCCCEEECCCCCCHHHHHHHHHHH
SGFQYQGIYGSNYHVKEKILMADLGFRVKQVIKTEVDVTQAQLTRNGVEVITGRAGFLDS
CCCEEECEECCCCEEHHEEEEHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
THVRVESSRGQTDLEAPIIIIATGTKPSTSPTVPLNGRTIVNSDQILDIPEIPKTLIVVG
CEEEEECCCCCCCCCCCEEEEECCCCCCCCCEECCCCCEEECCCCEECCCCCCCEEEEEC
GGVIGVEYTCIFAILGVRVILIEKRPRLLEFADSEMVEALSYHLRDHRVTMRLNEEVAAV
CCEEEHHHHHHHHHHHCEEEEEECCCCHHHHCCHHHHHHHHHHHCCCEEEEEECCHHHHH
EEQAGGGVVARLKSNKVISGDALLYAVGRQGNVEDLNLPAAGLEADGRGRITVDSDYRTK
HHHCCCCEEEEEECCEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCC
TSNIYAAGDVIGFPSLASVSMEQGRLAAANAVGLKIQSNPATYPYGIYTIPEISFVGKTE
CCCEEEECCCCCCCCHHHCCHHCCCEEEEEEEEEEEECCCCCCCEEEEECCCEEECCCCH
EQLTADDVPYEVGVAYYRETARGQIRGDTTGRLKLIFHRETREILGVHIIGEGATELLHI
HHCCCCCCCHHHHHHHHHHCCCCEEECCCCCEEEEEEEECCCEEEEEEEECCCHHHHHHH
GQAVMILKGTVDYFVETVFNYPTLAECYKQAAFNGINKLMRF
CCEEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQKFDIIVIGSGPAGQRAAIQGAKCGRRVAVIEQREIVGGACVNTGTIPSKTMREAVMHL
CCEEEEEEECCCCCCCCCEECCCCCCCEEEEEEHHHHCCCEEECCCCCCHHHHHHHHHHH
SGFQYQGIYGSNYHVKEKILMADLGFRVKQVIKTEVDVTQAQLTRNGVEVITGRAGFLDS
CCCEEECEECCCCEEHHEEEEHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
THVRVESSRGQTDLEAPIIIIATGTKPSTSPTVPLNGRTIVNSDQILDIPEIPKTLIVVG
CEEEEECCCCCCCCCCCEEEEECCCCCCCCCEECCCCCEEECCCCEECCCCCCCEEEEEC
GGVIGVEYTCIFAILGVRVILIEKRPRLLEFADSEMVEALSYHLRDHRVTMRLNEEVAAV
CCEEEHHHHHHHHHHHCEEEEEECCCCHHHHCCHHHHHHHHHHHCCCEEEEEECCHHHHH
EEQAGGGVVARLKSNKVISGDALLYAVGRQGNVEDLNLPAAGLEADGRGRITVDSDYRTK
HHHCCCCEEEEEECCEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCC
TSNIYAAGDVIGFPSLASVSMEQGRLAAANAVGLKIQSNPATYPYGIYTIPEISFVGKTE
CCCEEEECCCCCCCCHHHCCHHCCCEEEEEEEEEEEECCCCCCCEEEEECCCEEECCCCH
EQLTADDVPYEVGVAYYRETARGQIRGDTTGRLKLIFHRETREILGVHIIGEGATELLHI
HHCCCCCCCHHHHHHHHHHCCCCEEECCCCCEEEEEEEECCCEEEEEEEECCCHHHHHHH
GQAVMILKGTVDYFVETVFNYPTLAECYKQAAFNGINKLMRF
CCEEEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]