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The map label for this gene is folK [H]

Identifier: 116620086

GI number: 116620086

Start: 1196327

End: 1196809

Strand: Direct

Name: folK [H]

Synonym: Acid_0958

Alternate gene names: 116620086

Gene position: 1196327-1196809 (Clockwise)

Preceding gene: 116620085

Following gene: 116620087

Centisome position: 12.0

GC content: 66.67

Gene sequence:

>483_bases
ATGAAGCCGGTCTATCTTTCGCTCGGGTCCAACATCGGCGACCGCCACGCCAATCTGCGCGCCGCGATCGAGGCGCTCGA
TGCCGGCGGGGTACGTGTGCTTCGCACCTCGCCGGTTTATGAGACCGAGCCCGTGGATTATCGCGAGCAGGCATGGTTCC
TGAATCTGGTCGTGGAAGCCGAGACCACGCTGTTTCCCGTGCAGCTGCTCGCGCGGATTGGCCGCATCGAGCGCGCCCTC
GGCCGCGTTCGCAAGGTGGCCAAAGGTCCGCGCACGATCGATATCGACATCCTGTTCTACGGGTCCGCGATGGTGAAGAC
CGCGCATCTCGAGATCCCTCATCCGCGCCTGGCCGAGCGCCGCTTCGTGCTCGCGCCGCTCGCCGACCTGGTGCCGGACC
TGCGTCATCCGGCGACGCACCGCAGTGTGCGCGAGATGTTGCAAGATGCGCCGGCCGCTGCGGTGCGGCTGGTGCCGTCT
TAG

Upstream 100 bases:

>100_bases
ACCGCACGCCGCGCGGCCGCGTCGCTACGGAACTGGCCTACGACTACTTCAAGGTGAAGCGCAAGCTGCGCGAAGGCGAT
CACCCGAGCCTGTTCGAGGC

Downstream 100 bases:

>100_bases
GCGTGCGGCAGCGAGGATTGGCTGCCGGCGAGCCGCAAACTGGGAAACGGCTGGGATGAGGGGCGCGCGGTCGGCGGGGT
GCCGAGCATGAACGAAGGAG

Product: 2-amino-4-hydroxy-6- hydroxymethyldihydropteridine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 160; Mature: 160

Protein sequence:

>160_residues
MKPVYLSLGSNIGDRHANLRAAIEALDAGGVRVLRTSPVYETEPVDYREQAWFLNLVVEAETTLFPVQLLARIGRIERAL
GRVRKVAKGPRTIDIDILFYGSAMVKTAHLEIPHPRLAERRFVLAPLADLVPDLRHPATHRSVREMLQDAPAAAVRLVPS

Sequences:

>Translated_160_residues
MKPVYLSLGSNIGDRHANLRAAIEALDAGGVRVLRTSPVYETEPVDYREQAWFLNLVVEAETTLFPVQLLARIGRIERAL
GRVRKVAKGPRTIDIDILFYGSAMVKTAHLEIPHPRLAERRFVLAPLADLVPDLRHPATHRSVREMLQDAPAAAVRLVPS
>Mature_160_residues
MKPVYLSLGSNIGDRHANLRAAIEALDAGGVRVLRTSPVYETEPVDYREQAWFLNLVVEAETTLFPVQLLARIGRIERAL
GRVRKVAKGPRTIDIDILFYGSAMVKTAHLEIPHPRLAERRFVLAPLADLVPDLRHPATHRSVREMLQDAPAAAVRLVPS

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=139, Percent_Identity=38.1294964028777, Blast_Score=95, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6324073, Length=155, Percent_Identity=41.2903225806452, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 17816; Mature: 17816

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPVYLSLGSNIGDRHANLRAAIEALDAGGVRVLRTSPVYETEPVDYREQAWFLNLVVEA
CCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHEEEEEEEEC
ETTLFPVQLLARIGRIERALGRVRKVAKGPRTIDIDILFYGSAMVKTAHLEIPHPRLAER
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEEEEECCCCHHHHC
RFVLAPLADLVPDLRHPATHRSVREMLQDAPAAAVRLVPS
EEHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHEEECCC
>Mature Secondary Structure
MKPVYLSLGSNIGDRHANLRAAIEALDAGGVRVLRTSPVYETEPVDYREQAWFLNLVVEA
CCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHEEEEEEEEC
ETTLFPVQLLARIGRIERALGRVRKVAKGPRTIDIDILFYGSAMVKTAHLEIPHPRLAER
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCEEEEEEECCCCHHHHC
RFVLAPLADLVPDLRHPATHRSVREMLQDAPAAAVRLVPS
EEHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 2123867 [H]