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The map label for this gene is bfmBB [H]

Identifier: 116620042

GI number: 116620042

Start: 1141841

End: 1143169

Strand: Direct

Name: bfmBB [H]

Synonym: Acid_0914

Alternate gene names: 116620042

Gene position: 1141841-1143169 (Clockwise)

Preceding gene: 116620041

Following gene: 116620043

Centisome position: 11.46

GC content: 65.76

Gene sequence:

>1329_bases
ATGACCGACGTAGTGATGCCCCAGATGGGCGAAAGCATTGTAGAGGGTACCCTGACCAAGTGGCTGAAAAAGCCCGGCGA
GCGCATTGAACGCGATGAGCCGCTCTTTGAAATCTCGACCGACAAGGTGGATACCGAAATCCCGTCTCCGGCCGCCGGCA
CCCTCGCCGAAGTTCTGGTCGAGGAAGGGAAGACGGTCGGCATCAATACCGTGGTCGCCCGCATCAGCGAAGGGGGCAGC
GCCGATGGCGGCGCCGCCGCGGCCAAGCCCGTTGCCGCACCCGCTCCGCCGCCGCCCGCTGCAGCCGCGCCTCCGGCCGC
GCAGGCTCCCGTGGCGCCGCCCCCGCCGCCCGTCCAGGCCGCTCCTCCGCCGCCTCCCGCCGAGGCCGAATCTCCCTCCG
GGCCGCTTTCGCCGCTGGTGAAGAAGATGGCTCGCGAGATGAACCTCGATCTCGCGCAGGTGAAGGGCACGGGAGCTGGC
GGACGCATCACCAAGCAAGATGTAGAAGCCTATGTCGCGGCGGGCAAACCGGCTGCCGCTCCGCCGTCCCCCGCTCCGGC
TGCGACGCCCGTACAGGCTGCTGCCCCCGCGCCAACGCCTGCACCTGCGATGGCGCCCCTGGCGCCCGCCGGCCAGGCCA
AGACCCGCATCGAGCCGATGAGCACGATGCGCATCAAGATCGCCGAGCACATGGTGATGTCCAAGCGCACGTCGCCGCAC
GTCACCACCATTCACCGCGTCGATATGACCAAGGTCGCGAAGATGCGCGAGCGCTTCAAGGCGCAGTTCCAGGCCAACTA
CGGTTTCGGCCTCACGTACCTGCCCTTCATCACGCGCGCCACCGTCGCCGGCCTGCGCCAGTACCCGCTGCTCAATGCCT
CGCTCGACGGCAACAACATCATCTATCACAACGAAATTCACATCGGGATCGCGGTCGCGCTGGAAAACGGCCTGATCGTT
CCCGTGGTTCGTTCGGCCGATGAGAAGAACGTGCTCGGCCTGCAGCGCTCCATCGTGGATCTTGCCGCGCGCGCCCGCAG
CCGCCAGTTGAAGCCCGACGAAATTCAGGGCGGCACGTTCTCGATCACCAATTTCGGCAGCTTCGGTTCGCTCGTCGGAA
CCCCGGTCATCAATCAGCCGCAGGTCGCGATTCTCGGCGTCGGCACGGTGGACAAGACTCCCGTCGTGATCGACGACGCC
ATCGCCATCCGCAGCATCTGCCACCTGTCGCTCAGCTTCGATCACCGCCTGATCGATGGCGCCCTGGCCGATCAGTTCAT
GACGAAGGTCAAGCAGGTCCTCGAAAACTGGTCCGAAGAAGTTCTGTAG

Upstream 100 bases:

>100_bases
ATGAAGTGGGCGAACGCTTCACCCGACACTTCGCACGAATTTTCGATTTTGAAATGCTGCCCGGAGCCTCACTCGCGGCG
GCCTGAAAGAGAGAACCGAT

Downstream 100 bases:

>100_bases
CCGCGGCAGGCGCGTCCGCCCGCGGCGGCCGGCGAGGGGATGCGCCAGTTCCCGATTATTGCGCTAAAGTAAACGATGAC
CGAGGTGCCCATGTCGTTTA

Product: dihydrolipoyllysine-residue succinyltransferase

Products: NA

Alternate protein names: Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MTDVVMPQMGESIVEGTLTKWLKKPGERIERDEPLFEISTDKVDTEIPSPAAGTLAEVLVEEGKTVGINTVVARISEGGS
ADGGAAAAKPVAAPAPPPPAAAAPPAAQAPVAPPPPPVQAAPPPPPAEAESPSGPLSPLVKKMAREMNLDLAQVKGTGAG
GRITKQDVEAYVAAGKPAAAPPSPAPAATPVQAAAPAPTPAPAMAPLAPAGQAKTRIEPMSTMRIKIAEHMVMSKRTSPH
VTTIHRVDMTKVAKMRERFKAQFQANYGFGLTYLPFITRATVAGLRQYPLLNASLDGNNIIYHNEIHIGIAVALENGLIV
PVVRSADEKNVLGLQRSIVDLAARARSRQLKPDEIQGGTFSITNFGSFGSLVGTPVINQPQVAILGVGTVDKTPVVIDDA
IAIRSICHLSLSFDHRLIDGALADQFMTKVKQVLENWSEEVL

Sequences:

>Translated_442_residues
MTDVVMPQMGESIVEGTLTKWLKKPGERIERDEPLFEISTDKVDTEIPSPAAGTLAEVLVEEGKTVGINTVVARISEGGS
ADGGAAAAKPVAAPAPPPPAAAAPPAAQAPVAPPPPPVQAAPPPPPAEAESPSGPLSPLVKKMAREMNLDLAQVKGTGAG
GRITKQDVEAYVAAGKPAAAPPSPAPAATPVQAAAPAPTPAPAMAPLAPAGQAKTRIEPMSTMRIKIAEHMVMSKRTSPH
VTTIHRVDMTKVAKMRERFKAQFQANYGFGLTYLPFITRATVAGLRQYPLLNASLDGNNIIYHNEIHIGIAVALENGLIV
PVVRSADEKNVLGLQRSIVDLAARARSRQLKPDEIQGGTFSITNFGSFGSLVGTPVINQPQVAILGVGTVDKTPVVIDDA
IAIRSICHLSLSFDHRLIDGALADQFMTKVKQVLENWSEEVL
>Mature_441_residues
TDVVMPQMGESIVEGTLTKWLKKPGERIERDEPLFEISTDKVDTEIPSPAAGTLAEVLVEEGKTVGINTVVARISEGGSA
DGGAAAAKPVAAPAPPPPAAAAPPAAQAPVAPPPPPVQAAPPPPPAEAESPSGPLSPLVKKMAREMNLDLAQVKGTGAGG
RITKQDVEAYVAAGKPAAAPPSPAPAATPVQAAAPAPTPAPAMAPLAPAGQAKTRIEPMSTMRIKIAEHMVMSKRTSPHV
TTIHRVDMTKVAKMRERFKAQFQANYGFGLTYLPFITRATVAGLRQYPLLNASLDGNNIIYHNEIHIGIAVALENGLIVP
VVRSADEKNVLGLQRSIVDLAARARSRQLKPDEIQGGTFSITNFGSFGSLVGTPVINQPQVAILGVGTVDKTPVVIDDAI
AIRSICHLSLSFDHRLIDGALADQFMTKVKQVLENWSEEVL

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltran

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=439, Percent_Identity=33.0296127562642, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI19923748, Length=246, Percent_Identity=36.5853658536585, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI203098753, Length=451, Percent_Identity=30.5986696230599, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI203098816, Length=452, Percent_Identity=30.7522123893805, Blast_Score=172, Evalue=8e-43,
Organism=Homo sapiens, GI110671329, Length=434, Percent_Identity=27.8801843317972, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI260898739, Length=160, Percent_Identity=35.625, Blast_Score=92, Evalue=8e-19,
Organism=Escherichia coli, GI1786946, Length=434, Percent_Identity=33.6405529953917, Blast_Score=262, Evalue=3e-71,
Organism=Escherichia coli, GI1786305, Length=445, Percent_Identity=31.9101123595506, Blast_Score=176, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI17560088, Length=456, Percent_Identity=31.5789473684211, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI25146366, Length=214, Percent_Identity=37.3831775700935, Blast_Score=165, Evalue=5e-41,
Organism=Caenorhabditis elegans, GI17537937, Length=443, Percent_Identity=25.2821670428894, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17538894, Length=314, Percent_Identity=28.6624203821656, Blast_Score=111, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6320352, Length=439, Percent_Identity=30.2961275626424, Blast_Score=214, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6324258, Length=461, Percent_Identity=29.5010845986985, Blast_Score=167, Evalue=4e-42,
Organism=Drosophila melanogaster, GI24645909, Length=217, Percent_Identity=35.9447004608295, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI18859875, Length=436, Percent_Identity=26.8348623853211, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24582497, Length=227, Percent_Identity=32.5991189427313, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI20129315, Length=227, Percent_Identity=32.5991189427313, Blast_Score=122, Evalue=5e-28,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.168 [H]

Molecular weight: Translated: 46337; Mature: 46206

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVVMPQMGESIVEGTLTKWLKKPGERIERDEPLFEISTDKVDTEIPSPAAGTLAEVLV
CCCCCCHHHHHHHHHHHHHHHHHCHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHH
EEGKTVGINTVVARISEGGSADGGAAAAKPVAAPAPPPPAAAAPPAAQAPVAPPPPPVQA
HCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APPPPPAEAESPSGPLSPLVKKMAREMNLDLAQVKGTGAGGRITKQDVEAYVAAGKPAAA
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCC
PPSPAPAATPVQAAAPAPTPAPAMAPLAPAGQAKTRIEPMSTMRIKIAEHMVMSKRTSPH
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
VTTIHRVDMTKVAKMRERFKAQFQANYGFGLTYLPFITRATVAGLRQYPLLNASLDGNNI
EEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCEE
IYHNEIHIGIAVALENGLIVPVVRSADEKNVLGLQRSIVDLAARARSRQLKPDEIQGGTF
EEECCEEEEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
SITNFGSFGSLVGTPVINQPQVAILGVGTVDKTPVVIDDAIAIRSICHLSLSFDHRLIDG
EEECCCCCHHHHCCCCCCCCCEEEEECCCCCCCCEEECHHHHHHHHHHHHCCCCHHHHHH
ALADQFMTKVKQVLENWSEEVL
HHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDVVMPQMGESIVEGTLTKWLKKPGERIERDEPLFEISTDKVDTEIPSPAAGTLAEVLV
CCCCCHHHHHHHHHHHHHHHHHCHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHHHHHH
EEGKTVGINTVVARISEGGSADGGAAAAKPVAAPAPPPPAAAAPPAAQAPVAPPPPPVQA
HCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APPPPPAEAESPSGPLSPLVKKMAREMNLDLAQVKGTGAGGRITKQDVEAYVAAGKPAAA
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCC
PPSPAPAATPVQAAAPAPTPAPAMAPLAPAGQAKTRIEPMSTMRIKIAEHMVMSKRTSPH
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
VTTIHRVDMTKVAKMRERFKAQFQANYGFGLTYLPFITRATVAGLRQYPLLNASLDGNNI
EEEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCCCEE
IYHNEIHIGIAVALENGLIVPVVRSADEKNVLGLQRSIVDLAARARSRQLKPDEIQGGTF
EEECCEEEEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
SITNFGSFGSLVGTPVINQPQVAILGVGTVDKTPVVIDDAIAIRSICHLSLSFDHRLIDG
EEECCCCCHHHHCCCCCCCCCEEEEECCCCCCCCEEECHHHHHHHHHHHHCCCCHHHHHH
ALADQFMTKVKQVLENWSEEVL
HHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8504804; 8969508; 9384377; 7961792 [H]