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The map label for this gene is lipB

Identifier: 116620041

GI number: 116620041

Start: 1141164

End: 1141826

Strand: Direct

Name: lipB

Synonym: Acid_0913

Alternate gene names: 116620041

Gene position: 1141164-1141826 (Clockwise)

Preceding gene: 116620040

Following gene: 116620042

Centisome position: 11.45

GC content: 62.44

Gene sequence:

>663_bases
ATGCGTAATTGCTGCCTGCGCCAACTCGGGCGGATTGACTACGCGAGCGCGCTGGAAATCCAGGAGCAGCTCGCCGCAAG
CCGCAAACAGGGCACGGCCGCCGATCACCTGTTGCTGCTCGAGCACCCGCACGTCATCACGCTGGGACGCAACGGCCACC
TGGAGAATCTGCTCGCCGGCGACGATATCCTGGAGCGCGCAGGGATCGCATTCTTCCCCACGAATCGCGGCGGCGACGTC
ACCTACCACGGCCCGGGCCAATTGGTGGGCTACCCCATTCTCGATCTGCGCGATTGGAAGCGCGACGTCGGCGCCTACGT
CCGCGCCCTCGAACAGGCAATCATCGATACCCTGGGAGATTACGGCATCGAGGCCGGGCGGATTCCCAAACTCACGGGAG
TCTGGGTAGGCGAGCGCAAGATAGCCGCGATCGGCGTACACCTTAGCCGTTGGGTGACCTCGCACGGTTTCGCGCTCAAT
GTTTCCACCGATCTCAGCTATTTCCAATACATCGTGCCTTGCGGCCTGACCAAGCCCGTTACCTCCATGGCCGCGCTCGG
TGTACGCGCCTCATTGGATGAAGTGGGCGAACGCTTCACCCGACACTTCGCACGAATTTTCGATTTTGAAATGCTGCCCG
GAGCCTCACTCGCGGCGGCCTGA

Upstream 100 bases:

>100_bases
GAGGCTACCGTCGATGTCATGATGCAGACCATTCACGCCCATCCCACGCTCTACGAAGCGGTGGGCGAGGCCTTCAACGC
CGTTTACGGTCTCTCCATCA

Downstream 100 bases:

>100_bases
AAGAGAGAACCGATATGACCGACGTAGTGATGCCCCAGATGGGCGAAAGCATTGTAGAGGGTACCCTGACCAAGTGGCTG
AAAAAGCCCGGCGAGCGCAT

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MRNCCLRQLGRIDYASALEIQEQLAASRKQGTAADHLLLLEHPHVITLGRNGHLENLLAGDDILERAGIAFFPTNRGGDV
TYHGPGQLVGYPILDLRDWKRDVGAYVRALEQAIIDTLGDYGIEAGRIPKLTGVWVGERKIAAIGVHLSRWVTSHGFALN
VSTDLSYFQYIVPCGLTKPVTSMAALGVRASLDEVGERFTRHFARIFDFEMLPGASLAAA

Sequences:

>Translated_220_residues
MRNCCLRQLGRIDYASALEIQEQLAASRKQGTAADHLLLLEHPHVITLGRNGHLENLLAGDDILERAGIAFFPTNRGGDV
TYHGPGQLVGYPILDLRDWKRDVGAYVRALEQAIIDTLGDYGIEAGRIPKLTGVWVGERKIAAIGVHLSRWVTSHGFALN
VSTDLSYFQYIVPCGLTKPVTSMAALGVRASLDEVGERFTRHFARIFDFEMLPGASLAAA
>Mature_220_residues
MRNCCLRQLGRIDYASALEIQEQLAASRKQGTAADHLLLLEHPHVITLGRNGHLENLLAGDDILERAGIAFFPTNRGGDV
TYHGPGQLVGYPILDLRDWKRDVGAYVRALEQAIIDTLGDYGIEAGRIPKLTGVWVGERKIAAIGVHLSRWVTSHGFALN
VSTDLSYFQYIVPCGLTKPVTSMAALGVRASLDEVGERFTRHFARIFDFEMLPGASLAAA

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=220, Percent_Identity=37.7272727272727, Blast_Score=106, Evalue=1e-23,
Organism=Escherichia coli, GI87081767, Length=211, Percent_Identity=35.0710900473934, Blast_Score=115, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323268, Length=124, Percent_Identity=42.741935483871, Blast_Score=96, Evalue=4e-21,
Organism=Drosophila melanogaster, GI21355483, Length=218, Percent_Identity=38.9908256880734, Blast_Score=144, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_SOLUE (Q02AK4)

Other databases:

- EMBL:   CP000473
- RefSeq:   YP_822197.1
- ProteinModelPortal:   Q02AK4
- SMR:   Q02AK4
- STRING:   Q02AK4
- GeneID:   4430930
- GenomeReviews:   CP000473_GR
- KEGG:   sus:Acid_0913
- NMPDR:   fig|234267.9.peg.860
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   HPPVFTL
- PhylomeDB:   Q02AK4
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 24095; Mature: 24095

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 174-174

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNCCLRQLGRIDYASALEIQEQLAASRKQGTAADHLLLLEHPHVITLGRNGHLENLLAG
CCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEECCCCCHHHHHHH
DDILERAGIAFFPTNRGGDVTYHGPGQLVGYPILDLRDWKRDVGAYVRALEQAIIDTLGD
HHHHHHCCEEEEECCCCCCEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YGIEAGRIPKLTGVWVGERKIAAIGVHLSRWVTSHGFALNVSTDLSYFQYIVPCGLTKPV
CCCCCCCCCCEEEEEECCCEEEEHHHHHHHHHHHCCEEEEEECCHHHHHHHHCCCCCCHH
TSMAALGVRASLDEVGERFTRHFARIFDFEMLPGASLAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCC
>Mature Secondary Structure
MRNCCLRQLGRIDYASALEIQEQLAASRKQGTAADHLLLLEHPHVITLGRNGHLENLLAG
CCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEECCCCCHHHHHHH
DDILERAGIAFFPTNRGGDVTYHGPGQLVGYPILDLRDWKRDVGAYVRALEQAIIDTLGD
HHHHHHCCEEEEECCCCCCEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YGIEAGRIPKLTGVWVGERKIAAIGVHLSRWVTSHGFALNVSTDLSYFQYIVPCGLTKPV
CCCCCCCCCCEEEEEECCCEEEEHHHHHHHHHHHCCEEEEEECCHHHHHHHHCCCCCCHH
TSMAALGVRASLDEVGERFTRHFARIFDFEMLPGASLAAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA