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The map label for this gene is glcE [H]

Identifier: 116619590

GI number: 116619590

Start: 555137

End: 556285

Strand: Direct

Name: glcE [H]

Synonym: Acid_0451

Alternate gene names: 116619590

Gene position: 555137-556285 (Clockwise)

Preceding gene: 116619589

Following gene: 116619591

Centisome position: 5.57

GC content: 64.93

Gene sequence:

>1149_bases
GTGATCGCTCCTTCGAATTCTTCCGAACTGGCCGACGCCCTCCGCGCAGCGGCATCCGCCGGCCAAACCATCGCGCTCCA
CGGGAACTCCTCCAAGCGCCGCATGGCCGGCCCCATCGCGCCTGCCGACGTTGAAATCAGCACCCTATCCCTGCGCGGCG
TCATCCAATACGAGCCGCAGGATCTCACCATCAGCGTCGCCGCCGGACTCGCCTGGAGCGAACTCACCAGCCTCCTTGCC
GAACACCGCCAGATGGTCCCGCTCGACCCGCCGTTCGCCGGCGAAGCCACCGTCGGCGGCGTCATCGCCGCCAATTGCAG
CGGTCCGCGCCGGCGCCTCTACGGCACCGCCCGCGATTTGGTTATCGGCATGCAATTCGTCACCCTGGAAGGCAAGCTCG
TCCAGTCCGGCGGCATGGTCGTCAAGAATGTCGCCGGCCTCGATATGGCCAAGCTCATGATCGGCTCATTCGGCACTCTC
GCCGCCATCGGCGTCGTCAATTTCAAGCTCCAGCCGGTGCCGCACATGGAACGCAGCTTCCTCCTGCCGTTCGCGGGACT
CCCCGAGGCCATCGCCGCCCGTAATCTCATCCTCCGCGGCCAACTGCAGCCCGCCGCCATCGATCTGCTCAATCCCGCCG
CTGCCCGCACGCTCGGCAACTCATCCTGGCTGCTCGCCCTGCGCGCCGCCGGCAACACTGCCGCCGTGGACCGCTACGAA
CGCGAATTCGCCGGGCTCCCCGACGCCCTGGCCTTTGAAAACGATCGCCACCAGACCCTCTGGCGGCACATCGAGAATTT
CACGCCGTCTTTCCTCAACCAGCACAAGGATGGCGTCGTCGTGCGCGCTTCGTGTACGCTAAAGGAATCCGAAGCGGTCA
TGGAGTCCTTCCCCGGTCCGGCAATTGCCCGCGCCGGCAACGGTATCTGCTATGGCTATTTCGAAGATGCGCGGGCCGCC
GCGGAGTGGATGGCCGGTGCCGCCGGACGCAACGCCAAAGTCGTTGTCGAGTTCGCCCCGGATCGCGTCCGCGGTGAAAT
GGAACTCTGGCCCTCAGTGGGCCCCGATTTTGAAATTATGAAACGGGTCAAGAATCTCTTCGACCCCCGGAACCTTCTGA
ACCGTGGCAGACTCTACCGCCGTATCTGA

Upstream 100 bases:

>100_bases
GATTCCGCATCGATGGTTTCCTCGCCGCGCTGCTCGGCTCGCTCGTCCTGGCTATCGTCACCGGCCTTCTCCAAAGTCTG
GCCTGGGCCACCATTTAACC

Downstream 100 bases:

>100_bases
AAAGACCGATCTCGGGCCCAGGCAGTTCGATCTCGATAAGTGTGTCCACTGCGGTCTCTGCCTCAACGCCTGCCCCACCT
ACCGTGAGCTCGGCCTCGAA

Product: FAD linked oxidase domain-containing protein

Products: 2-oxo acid; H2O2

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MIAPSNSSELADALRAAASAGQTIALHGNSSKRRMAGPIAPADVEISTLSLRGVIQYEPQDLTISVAAGLAWSELTSLLA
EHRQMVPLDPPFAGEATVGGVIAANCSGPRRRLYGTARDLVIGMQFVTLEGKLVQSGGMVVKNVAGLDMAKLMIGSFGTL
AAIGVVNFKLQPVPHMERSFLLPFAGLPEAIAARNLILRGQLQPAAIDLLNPAAARTLGNSSWLLALRAAGNTAAVDRYE
REFAGLPDALAFENDRHQTLWRHIENFTPSFLNQHKDGVVVRASCTLKESEAVMESFPGPAIARAGNGICYGYFEDARAA
AEWMAGAAGRNAKVVVEFAPDRVRGEMELWPSVGPDFEIMKRVKNLFDPRNLLNRGRLYRRI

Sequences:

>Translated_382_residues
MIAPSNSSELADALRAAASAGQTIALHGNSSKRRMAGPIAPADVEISTLSLRGVIQYEPQDLTISVAAGLAWSELTSLLA
EHRQMVPLDPPFAGEATVGGVIAANCSGPRRRLYGTARDLVIGMQFVTLEGKLVQSGGMVVKNVAGLDMAKLMIGSFGTL
AAIGVVNFKLQPVPHMERSFLLPFAGLPEAIAARNLILRGQLQPAAIDLLNPAAARTLGNSSWLLALRAAGNTAAVDRYE
REFAGLPDALAFENDRHQTLWRHIENFTPSFLNQHKDGVVVRASCTLKESEAVMESFPGPAIARAGNGICYGYFEDARAA
AEWMAGAAGRNAKVVVEFAPDRVRGEMELWPSVGPDFEIMKRVKNLFDPRNLLNRGRLYRRI
>Mature_382_residues
MIAPSNSSELADALRAAASAGQTIALHGNSSKRRMAGPIAPADVEISTLSLRGVIQYEPQDLTISVAAGLAWSELTSLLA
EHRQMVPLDPPFAGEATVGGVIAANCSGPRRRLYGTARDLVIGMQFVTLEGKLVQSGGMVVKNVAGLDMAKLMIGSFGTL
AAIGVVNFKLQPVPHMERSFLLPFAGLPEAIAARNLILRGQLQPAAIDLLNPAAARTLGNSSWLLALRAAGNTAAVDRYE
REFAGLPDALAFENDRHQTLWRHIENFTPSFLNQHKDGVVVRASCTLKESEAVMESFPGPAIARAGNGICYGYFEDARAA
AEWMAGAAGRNAKVVVEFAPDRVRGEMELWPSVGPDFEIMKRVKNLFDPRNLLNRGRLYRRI

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI4501993, Length=180, Percent_Identity=26.6666666666667, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI119964728, Length=421, Percent_Identity=22.3277909738717, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI48994914, Length=379, Percent_Identity=27.7044854881267, Blast_Score=118, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17556096, Length=185, Percent_Identity=23.7837837837838, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71992381, Length=255, Percent_Identity=25.8823529411765, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71992373, Length=285, Percent_Identity=22.8070175438596, Blast_Score=65, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71992392, Length=240, Percent_Identity=25.8333333333333, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320027, Length=214, Percent_Identity=27.1028037383178, Blast_Score=87, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6320764, Length=432, Percent_Identity=21.5277777777778, Blast_Score=71, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320023, Length=129, Percent_Identity=31.0077519379845, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24653753, Length=152, Percent_Identity=27.6315789473684, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF01565 FAD_binding_4 [H]

EC number: 1.1.3.15

Molecular weight: Translated: 41139; Mature: 41139

Theoretical pI: Translated: 8.81; Mature: 8.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAPSNSSELADALRAAASAGQTIALHGNSSKRRMAGPIAPADVEISTLSLRGVIQYEPQ
CCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEEEEEEEECCC
DLTISVAAGLAWSELTSLLAEHRQMVPLDPPFAGEATVGGVIAANCSGPRRRLYGTARDL
CEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHCHHHH
VIGMQFVTLEGKLVQSGGMVVKNVAGLDMAKLMIGSFGTLAAIGVVNFKLQPVPHMERSF
HEEEEEEEECCEEEECCCEEEEHHCCCHHHHHHHCCCHHHHHHHEEEEEECCCCCCCHHE
LLPFAGLPEAIAARNLILRGQLQPAAIDLLNPAAARTLGNSSWLLALRAAGNTAAVDRYE
ECCCCCCCHHHHHCCEEEECCCCHHHHHHCCHHHHHHCCCCCEEEEEEECCCCHHHHHHH
REFAGLPDALAFENDRHQTLWRHIENFTPSFLNQHKDGVVVRASCTLKESEAVMESFPGP
HHHCCCCHHHHCCCCHHHHHHHHHHHCCHHHHHCCCCCEEEEEEECCCHHHHHHHHCCCC
AIARAGNGICYGYFEDARAAAEWMAGAAGRNAKVVVEFAPDRVRGEMELWPSVGPDFEIM
HHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCEEEEEECCCHHCCCEEECCCCCCCHHHH
KRVKNLFDPRNLLNRGRLYRRI
HHHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MIAPSNSSELADALRAAASAGQTIALHGNSSKRRMAGPIAPADVEISTLSLRGVIQYEPQ
CCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEEEEEEEEECCC
DLTISVAAGLAWSELTSLLAEHRQMVPLDPPFAGEATVGGVIAANCSGPRRRLYGTARDL
CEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHCHHHH
VIGMQFVTLEGKLVQSGGMVVKNVAGLDMAKLMIGSFGTLAAIGVVNFKLQPVPHMERSF
HEEEEEEEECCEEEECCCEEEEHHCCCHHHHHHHCCCHHHHHHHEEEEEECCCCCCCHHE
LLPFAGLPEAIAARNLILRGQLQPAAIDLLNPAAARTLGNSSWLLALRAAGNTAAVDRYE
ECCCCCCCHHHHHCCEEEECCCCHHHHHHCCHHHHHHCCCCCEEEEEEECCCCHHHHHHH
REFAGLPDALAFENDRHQTLWRHIENFTPSFLNQHKDGVVVRASCTLKESEAVMESFPGP
HHHCCCCHHHHCCCCHHHHHHHHHHHCCHHHHHCCCCCEEEEEEECCCHHHHHHHHCCCC
AIARAGNGICYGYFEDARAAAEWMAGAAGRNAKVVVEFAPDRVRGEMELWPSVGPDFEIM
HHHHCCCCEEEEEHHHHHHHHHHHHCCCCCCCEEEEEECCCHHCCCEEECCCCCCCHHHH
KRVKNLFDPRNLLNRGRLYRRI
HHHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (S)-2-hydroxy acid; O2

Specific reaction: (S)-2-hydroxy acid + O2 = 2-oxo acid + H2O2

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]