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The map label for this gene is glcF [H]

Identifier: 116619591

GI number: 116619591

Start: 556260

End: 557561

Strand: Direct

Name: glcF [H]

Synonym: Acid_0452

Alternate gene names: 116619591

Gene position: 556260-557561 (Clockwise)

Preceding gene: 116619590

Following gene: 116619593

Centisome position: 5.58

GC content: 63.98

Gene sequence:

>1302_bases
GTGGCAGACTCTACCGCCGTATCTGAAAAGACCGATCTCGGGCCCAGGCAGTTCGATCTCGATAAGTGTGTCCACTGCGG
TCTCTGCCTCAACGCCTGCCCCACCTACCGTGAGCTCGGCCTCGAAATGGATTCCCCGCGCGGCCGCGTCTACCAGATGG
TCCAGGTCGCCGCCGGGGCCCCCATCACCAGCTCCTACCGGGAACACATCGATCTCTGCCTCGCCTGCCGCGGCTGCGAA
TCGGCCTGCCCCTCCGGCGTCCCCTACGGCCGCATGGTGGAGCACGCGCGCGCCGAAATCGAGCAGCACAGTACCCGCGG
CTGGCTCGCCACCAAGCTGCGCAACTTTGCCTTCCTCCACGTCCTGCCCTCGCGCAGCGCCCTTTCCGTCGCCGGAACTC
TGCTCTATCTCTTCGAAGTCAGCGGCCTCAAAGCCCTCGCGCGCGGCCTCGGCCAAATGAGACTGCTTGGCCCACTTAGT
GACCTGCAACAGCTTACGCCCTCTGCCGAGCCGCCCTTCTTCTTCAGCCAGATCGGACGCACTTTTCCCCCCGAAGGCGA
ACGCCGCCATCGGGTCGCATTCCTGGCCGGCTGCATCGCCAATGTCAGCTTCGCGCGCTTGAACGAGGCCACCATCCGCG
TGCTGCAGCGCAATGGCTGCGAAGTCGTCGTCCCGGAAAATCAGGGCTGCTGCGGCGCCCTCCACCTCCACTCCGGCATG
CGCGACGAGGCCCGTAAACTCGCCATCCGCAATATCGACGCCGTCCTCAGCGGCGGCTTCGACGCCATCATCACCAACGC
CGCCGGCTGCGGTTCCACCCTCAAGGAGTACGACGAGCTGCTCGCCGATCTCCCCGAGTATGCCGAAAAGGCCCGCGAAT
TCTCGCGCCTCATGCGCGACATCACCGAGTTCCTGTCGGGCCTCGAGTTGAACCCGAACCTCGGCCCCGTCGATGCCGTG
GTCACCTATCAGGATTCCTGCCACCTGGCCCACGGACAGCGCATCCGCAAATCCCCTCGTCAGTTGCTCGCCGCCATTCC
AGGACTGACCTTCGTCGAAATGTCCGGTCCCGATATCTGCTGCGGCAGCGCCGGCGTCTACAACGTCGTACAGAATGAAA
TGGCCATGCAGATTCTGGCCTCAAAAATGAAATCCGTGAATGCCACTCGCGCCGGCATCATCGCCACGGCCAACCCCGGC
TGCATGCTCCAGTTGCAAGCCGGAGTCCGCCTCCACGGCTCCTCCCAACGCGTCATGCATGTCGTCGAACTGCTCGACAT
CGCCTACAAAAACTTCCGCTAG

Upstream 100 bases:

>100_bases
CGCGTCCGCGGTGAAATGGAACTCTGGCCCTCAGTGGGCCCCGATTTTGAAATTATGAAACGGGTCAAGAATCTCTTCGA
CCCCCGGAACCTTCTGAACC

Downstream 100 bases:

>100_bases
GTCATTGACCGTGCTTTTTGTGAGCGGCGCTTAGTCTCGCGGCGTCCTTTAGTCAGTCAGCCGCCACGCGCTCCTGATCC
TTATATTGCAGCTGATATAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MADSTAVSEKTDLGPRQFDLDKCVHCGLCLNACPTYRELGLEMDSPRGRVYQMVQVAAGAPITSSYREHIDLCLACRGCE
SACPSGVPYGRMVEHARAEIEQHSTRGWLATKLRNFAFLHVLPSRSALSVAGTLLYLFEVSGLKALARGLGQMRLLGPLS
DLQQLTPSAEPPFFFSQIGRTFPPEGERRHRVAFLAGCIANVSFARLNEATIRVLQRNGCEVVVPENQGCCGALHLHSGM
RDEARKLAIRNIDAVLSGGFDAIITNAAGCGSTLKEYDELLADLPEYAEKAREFSRLMRDITEFLSGLELNPNLGPVDAV
VTYQDSCHLAHGQRIRKSPRQLLAAIPGLTFVEMSGPDICCGSAGVYNVVQNEMAMQILASKMKSVNATRAGIIATANPG
CMLQLQAGVRLHGSSQRVMHVVELLDIAYKNFR

Sequences:

>Translated_433_residues
MADSTAVSEKTDLGPRQFDLDKCVHCGLCLNACPTYRELGLEMDSPRGRVYQMVQVAAGAPITSSYREHIDLCLACRGCE
SACPSGVPYGRMVEHARAEIEQHSTRGWLATKLRNFAFLHVLPSRSALSVAGTLLYLFEVSGLKALARGLGQMRLLGPLS
DLQQLTPSAEPPFFFSQIGRTFPPEGERRHRVAFLAGCIANVSFARLNEATIRVLQRNGCEVVVPENQGCCGALHLHSGM
RDEARKLAIRNIDAVLSGGFDAIITNAAGCGSTLKEYDELLADLPEYAEKAREFSRLMRDITEFLSGLELNPNLGPVDAV
VTYQDSCHLAHGQRIRKSPRQLLAAIPGLTFVEMSGPDICCGSAGVYNVVQNEMAMQILASKMKSVNATRAGIIATANPG
CMLQLQAGVRLHGSSQRVMHVVELLDIAYKNFR
>Mature_432_residues
ADSTAVSEKTDLGPRQFDLDKCVHCGLCLNACPTYRELGLEMDSPRGRVYQMVQVAAGAPITSSYREHIDLCLACRGCES
ACPSGVPYGRMVEHARAEIEQHSTRGWLATKLRNFAFLHVLPSRSALSVAGTLLYLFEVSGLKALARGLGQMRLLGPLSD
LQQLTPSAEPPFFFSQIGRTFPPEGERRHRVAFLAGCIANVSFARLNEATIRVLQRNGCEVVVPENQGCCGALHLHSGMR
DEARKLAIRNIDAVLSGGFDAIITNAAGCGSTLKEYDELLADLPEYAEKAREFSRLMRDITEFLSGLELNPNLGPVDAVV
TYQDSCHLAHGQRIRKSPRQLLAAIPGLTFVEMSGPDICCGSAGVYNVVQNEMAMQILASKMKSVNATRAGIIATANPGC
MLQLQAGVRLHGSSQRVMHVVELLDIAYKNFR

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=438, Percent_Identity=31.9634703196347, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1786497, Length=240, Percent_Identity=30.4166666666667, Blast_Score=124, Evalue=2e-29,
Organism=Escherichia coli, GI1788576, Length=428, Percent_Identity=26.1682242990654, Blast_Score=106, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 47150; Mature: 47019

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADSTAVSEKTDLGPRQFDLDKCVHCGLCLNACPTYRELGLEMDSPRGRVYQMVQVAAGA
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHCCC
PITSSYREHIDLCLACRGCESACPSGVPYGRMVEHARAEIEQHSTRGWLATKLRNFAFLH
CCHHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEE
VLPSRSALSVAGTLLYLFEVSGLKALARGLGQMRLLGPLSDLQQLTPSAEPPFFFSQIGR
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEHHHCCHHHHHHHCCCCCCCHHHHHHCC
TFPPEGERRHRVAFLAGCIANVSFARLNEATIRVLQRNGCEVVVPENQGCCGALHLHSGM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEHHHHCCC
RDEARKLAIRNIDAVLSGGFDAIITNAAGCGSTLKEYDELLADLPEYAEKAREFSRLMRD
HHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITEFLSGLELNPNLGPVDAVVTYQDSCHLAHGQRIRKSPRQLLAAIPGLTFVEMSGPDIC
HHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEE
CGSAGVYNVVQNEMAMQILASKMKSVNATRAGIIATANPGCMLQLQAGVRLHGSSQRVMH
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCEECCCHHHHHH
VVELLDIAYKNFR
HHHHHHHHHHCCC
>Mature Secondary Structure 
ADSTAVSEKTDLGPRQFDLDKCVHCGLCLNACPTYRELGLEMDSPRGRVYQMVQVAAGA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHCCC
PITSSYREHIDLCLACRGCESACPSGVPYGRMVEHARAEIEQHSTRGWLATKLRNFAFLH
CCHHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEEE
VLPSRSALSVAGTLLYLFEVSGLKALARGLGQMRLLGPLSDLQQLTPSAEPPFFFSQIGR
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEHHHCCHHHHHHHCCCCCCCHHHHHHCC
TFPPEGERRHRVAFLAGCIANVSFARLNEATIRVLQRNGCEVVVPENQGCCGALHLHSGM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCEEEHHHHCCC
RDEARKLAIRNIDAVLSGGFDAIITNAAGCGSTLKEYDELLADLPEYAEKAREFSRLMRD
HHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ITEFLSGLELNPNLGPVDAVVTYQDSCHLAHGQRIRKSPRQLLAAIPGLTFVEMSGPDIC
HHHHHCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEE
CGSAGVYNVVQNEMAMQILASKMKSVNATRAGIIATANPGCMLQLQAGVRLHGSSQRVMH
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEECCCEECCCHHHHHH
VVELLDIAYKNFR
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]