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The map label for this gene is ntrB [H]

Identifier: 116619577

GI number: 116619577

Start: 544820

End: 546220

Strand: Direct

Name: ntrB [H]

Synonym: Acid_0438

Alternate gene names: 116619577

Gene position: 544820-546220 (Clockwise)

Preceding gene: 116619576

Following gene: 116619578

Centisome position: 5.47

GC content: 64.6

Gene sequence:

>1401_bases
ATGAGCGACCTCGCCGCGGAATTGCGCGCCATCCCCGCTTTCTCGGAGCTCACCGGCGACGCGATCGATTGGCTCGCCGC
CCACATGGAGACCCTCTCCTTCGCGGAAGGAGAAGTCGTCGCCGCCGAAGGGTCCGCCGCCGACCGCATGATCGTCGTGC
TGGAGGGCGAGCTGCAAGGCCGAACCGAGCACGGGCCAAGCGGAGGCCGGATCTTCATCACCCGTGCTGGAGACGTGAGC
GGCCTGCTGCCTTACTCGCGCCTGACCCACTATCCCACCACCATTCGCGCTATGGCGCGCTCACGGCTCGCCACCTTGCA
CTCCAGTCATTTTCCGGAAATGGTCGAACGCTTTCCCGCGCTTGCCGGGCGCCTGGTGGGCATCATGGCGGATCGCATCC
GCTATGTCACGCGCGACGAACAGCAGCGCGAAAAAATGATGGCGCTCGGCAAGCTCTCGGCTGGGCTCGCCCACGAGCTC
AATAACCCGGCTTCCGCGGCTTCCGTCGCCGCCGAGAACCTGCGTGAAGCCGTGTGCAGAATGCGCGCCGCCGACCTGGC
GATCGATCGCCTGATGCTTCCCGCCGACCAGCGCCTGACGCTCTCCGAGGCTGAGCAGGGATTGCTGGAAACGCCACCGC
CGCAGGCGATCTCCATCGACCCGCTGGAGCGCAGCGACCGGGAACAGGCCATCGGCGAGTGGCTCGACGAGCGTGGGATG
GAGGACGCCTGGAAGCTTGCCGGCGTGCTCGCCGACGCCGGCTTCGATCATGCGAGGCTGGAGAGACTCACCGCGGGTTT
CCCGGCTGACCTTCTGGCTCCCGTGGTGGAGCGCTTCGCCGCCGCGCTCTCCGTCACGCGCTTGGTGGAGGTCATCGAAA
GCAGCTCGCGACGCATGTCGCAACTGGTCAAAGCCGTCAAGGAATACTCTTACATGGACCAGAGTTCGGAGCAGGAAATC
GATGTCCATGACGGCCTGGAGAATACCCTGCTGATTCTCGGCCACCGCCTCAAACGCGGTATTCAGGTCACCCGCGAGTT
CGACCGCGGGCTGCCCCGCATCTGCGCCTTTGGCAGCGAACTCAACCAGGTATGGACCAACCTGATTGTCAACGCGATCG
ATGCCATGGGCGAAAACGGGGAACTGAAGATTCACACGGCGCGGGAAGGCGACGCGGTGTTGGTGGAGATCGTGGATTCC
GGCCCCGGCATTCCCGATGAGGCAAAGTCGCACATCTTCGAGCCGTTCTTCACCACCAAACCGGTAGGGCAGGGCACCGG
CTTAGGATTGGACACGGCCAACCGCATTGTACGGAAGCATCGAGGCTCGATTCGCTTCGAGAGCCGCCCGGGACGCACGG
CGTTCCAGGTCCGCCTTCCTTACCCAAAGGTGGCCGCCTGA

Upstream 100 bases:

>100_bases
GCGATGTGCGCGCCGGGTCGGTCAAGCGTGTTGCCTCCGGCGTGGGAGAGGGTTCTATCGCCATTCAATTCGTTCACCAG
TACCTGGCGAAGGTGGCGGC

Downstream 100 bases:

>100_bases
ACGCAACTGGCCCTTAACTTGCATCGAACGGAGATGTGTTCTCTGTCGATCCTGCCGAATCCGCACAGGAAGCGGGCCTG
CGCTACGTATCCGGATGCGG

Product: cyclic nucleotide-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MSDLAAELRAIPAFSELTGDAIDWLAAHMETLSFAEGEVVAAEGSAADRMIVVLEGELQGRTEHGPSGGRIFITRAGDVS
GLLPYSRLTHYPTTIRAMARSRLATLHSSHFPEMVERFPALAGRLVGIMADRIRYVTRDEQQREKMMALGKLSAGLAHEL
NNPASAASVAAENLREAVCRMRAADLAIDRLMLPADQRLTLSEAEQGLLETPPPQAISIDPLERSDREQAIGEWLDERGM
EDAWKLAGVLADAGFDHARLERLTAGFPADLLAPVVERFAAALSVTRLVEVIESSSRRMSQLVKAVKEYSYMDQSSEQEI
DVHDGLENTLLILGHRLKRGIQVTREFDRGLPRICAFGSELNQVWTNLIVNAIDAMGENGELKIHTAREGDAVLVEIVDS
GPGIPDEAKSHIFEPFFTTKPVGQGTGLGLDTANRIVRKHRGSIRFESRPGRTAFQVRLPYPKVAA

Sequences:

>Translated_466_residues
MSDLAAELRAIPAFSELTGDAIDWLAAHMETLSFAEGEVVAAEGSAADRMIVVLEGELQGRTEHGPSGGRIFITRAGDVS
GLLPYSRLTHYPTTIRAMARSRLATLHSSHFPEMVERFPALAGRLVGIMADRIRYVTRDEQQREKMMALGKLSAGLAHEL
NNPASAASVAAENLREAVCRMRAADLAIDRLMLPADQRLTLSEAEQGLLETPPPQAISIDPLERSDREQAIGEWLDERGM
EDAWKLAGVLADAGFDHARLERLTAGFPADLLAPVVERFAAALSVTRLVEVIESSSRRMSQLVKAVKEYSYMDQSSEQEI
DVHDGLENTLLILGHRLKRGIQVTREFDRGLPRICAFGSELNQVWTNLIVNAIDAMGENGELKIHTAREGDAVLVEIVDS
GPGIPDEAKSHIFEPFFTTKPVGQGTGLGLDTANRIVRKHRGSIRFESRPGRTAFQVRLPYPKVAA
>Mature_465_residues
SDLAAELRAIPAFSELTGDAIDWLAAHMETLSFAEGEVVAAEGSAADRMIVVLEGELQGRTEHGPSGGRIFITRAGDVSG
LLPYSRLTHYPTTIRAMARSRLATLHSSHFPEMVERFPALAGRLVGIMADRIRYVTRDEQQREKMMALGKLSAGLAHELN
NPASAASVAAENLREAVCRMRAADLAIDRLMLPADQRLTLSEAEQGLLETPPPQAISIDPLERSDREQAIGEWLDERGME
DAWKLAGVLADAGFDHARLERLTAGFPADLLAPVVERFAAALSVTRLVEVIESSSRRMSQLVKAVKEYSYMDQSSEQEID
VHDGLENTLLILGHRLKRGIQVTREFDRGLPRICAFGSELNQVWTNLIVNAIDAMGENGELKIHTAREGDAVLVEIVDSG
PGIPDEAKSHIFEPFFTTKPVGQGTGLGLDTANRIVRKHRGSIRFESRPGRTAFQVRLPYPKVAA

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=186, Percent_Identity=31.7204301075269, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788549, Length=165, Percent_Identity=33.3333333333333, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1790300, Length=153, Percent_Identity=30.718954248366, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI87082128, Length=189, Percent_Identity=27.5132275132275, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI87081816, Length=140, Percent_Identity=33.5714285714286, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51069; Mature: 50938

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50042 CNMP_BINDING_3 ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLAAELRAIPAFSELTGDAIDWLAAHMETLSFAEGEVVAAEGSAADRMIVVLEGELQG
CCCHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEECCCCC
RTEHGPSGGRIFITRAGDVSGLLPYSRLTHYPTTIRAMARSRLATLHSSHFPEMVERFPA
CCCCCCCCCEEEEEECCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LAGRLVGIMADRIRYVTRDEQQREKMMALGKLSAGLAHELNNPASAASVAAENLREAVCR
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
MRAADLAIDRLMLPADQRLTLSEAEQGLLETPPPQAISIDPLERSDREQAIGEWLDERGM
HHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCC
EDAWKLAGVLADAGFDHARLERLTAGFPADLLAPVVERFAAALSVTRLVEVIESSSRRMS
HHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLVKAVKEYSYMDQSSEQEIDVHDGLENTLLILGHRLKRGIQVTREFDRGLPRICAFGSE
HHHHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LNQVWTNLIVNAIDAMGENGELKIHTAREGDAVLVEIVDSGPGIPDEAKSHIFEPFFTTK
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHCCCCCCCC
PVGQGTGLGLDTANRIVRKHRGSIRFESRPGRTAFQVRLPYPKVAA
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure 
SDLAAELRAIPAFSELTGDAIDWLAAHMETLSFAEGEVVAAEGSAADRMIVVLEGELQG
CCHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEEEEECCCCC
RTEHGPSGGRIFITRAGDVSGLLPYSRLTHYPTTIRAMARSRLATLHSSHFPEMVERFPA
CCCCCCCCCEEEEEECCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LAGRLVGIMADRIRYVTRDEQQREKMMALGKLSAGLAHELNNPASAASVAAENLREAVCR
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
MRAADLAIDRLMLPADQRLTLSEAEQGLLETPPPQAISIDPLERSDREQAIGEWLDERGM
HHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCC
EDAWKLAGVLADAGFDHARLERLTAGFPADLLAPVVERFAAALSVTRLVEVIESSSRRMS
HHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLVKAVKEYSYMDQSSEQEIDVHDGLENTLLILGHRLKRGIQVTREFDRGLPRICAFGSE
HHHHHHHHHHHCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LNQVWTNLIVNAIDAMGENGELKIHTAREGDAVLVEIVDSGPGIPDEAKSHIFEPFFTTK
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEEEEECCCCCCCHHHHHHHCCCCCCCC
PVGQGTGLGLDTANRIVRKHRGSIRFESRPGRTAFQVRLPYPKVAA
CCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2710108; 8355615 [H]