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The map label for this gene is nagB

Identifier: 116496305

GI number: 116496305

Start: 2871558

End: 2872268

Strand: Direct

Name: nagB

Synonym: LSEI_2889

Alternate gene names: 116496305

Gene position: 2871558-2872268 (Clockwise)

Preceding gene: 116496299

Following gene: 116496306

Centisome position: 99.18

GC content: 46.69

Gene sequence:

>711_bases
ATGGATGTAAAAATTTTTGATAACGATACAGAAGCAGGCAAGTACGCATTTGATTTGATCAAGCAAGGTATGGATAACGG
TGCCAAAGTGCTCGGCTTGGCTACCGGCAGCACACCAGTCACCATGTACAAAGCAATGGTTAATAGTGATGTTGATTTCA
GCAACATGACTTCCATTAACTTGGATGAATATGTTGGGTTGGCTCCAGACAATGACCAGAGCTACCGTTACTTCATGCAG
AGTAACCTCTTCGACAAAAAGCCGTTCAAGGAAACCTTTGTTCCAAACGGCTTGGCTAAGGGTCCTGAAGAAGAAACCAC
GCGTTACAACAAAGTAATCGCCGACCATCCGATCAACATCCAAGTACTTGGCATTGGCCGTAACGGTCACATTGGCTTCA
ACGAACCCGGTTCACCATTTGACGCAGAAACCCGCAAAGTACCGCTGACCCAAAGCACCATTGACGCTAACGCCCGGTTC
TTTGCAAGTGAAGATGACGTACCTCGCTACGCCTACTCCATGGGGATCGGCTCAATCTTGAAGAGCAAGAAGATTTTGCT
GTTAGCCTTTGGCGAAAACAAGGCAGATGCTGTCAAAAAAATGATTGAAGGCCCTGTCACCAACGATGTGCCAGCGTCCG
CACTGCAAAAGCATTCAGATGTTGTTGTCATCTTGGACAAAGCAGCTGCAAGCAAGCTGAGCAAGAAATAA

Upstream 100 bases:

>100_bases
CACAGAAAATCAAAATTTTCGGCATTACGGTATAGACCACTATTGACATGGAGGTTTTTTGATGCTACATTGGACTAGAC
CAGATAGGGGGATATCAATG

Downstream 100 bases:

>100_bases
GCTATTTCTTTCAATCAGCTGAGATGAAACAGGCCGATGCTCATTGGCCTGTTTTTTTATGGAAGTGTAGCTTAACCCAG
TTAGAAAACGGCGTGTAAGC

Product: glucosamine-6-phosphate isomerase

Products: NA

Alternate protein names: GlcN6P deaminase; GNPDA; Glucosamine-6-phosphate isomerase

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MDVKIFDNDTEAGKYAFDLIKQGMDNGAKVLGLATGSTPVTMYKAMVNSDVDFSNMTSINLDEYVGLAPDNDQSYRYFMQ
SNLFDKKPFKETFVPNGLAKGPEEETTRYNKVIADHPINIQVLGIGRNGHIGFNEPGSPFDAETRKVPLTQSTIDANARF
FASEDDVPRYAYSMGIGSILKSKKILLLAFGENKADAVKKMIEGPVTNDVPASALQKHSDVVVILDKAAASKLSKK

Sequences:

>Translated_236_residues
MDVKIFDNDTEAGKYAFDLIKQGMDNGAKVLGLATGSTPVTMYKAMVNSDVDFSNMTSINLDEYVGLAPDNDQSYRYFMQ
SNLFDKKPFKETFVPNGLAKGPEEETTRYNKVIADHPINIQVLGIGRNGHIGFNEPGSPFDAETRKVPLTQSTIDANARF
FASEDDVPRYAYSMGIGSILKSKKILLLAFGENKADAVKKMIEGPVTNDVPASALQKHSDVVVILDKAAASKLSKK
>Mature_236_residues
MDVKIFDNDTEAGKYAFDLIKQGMDNGAKVLGLATGSTPVTMYKAMVNSDVDFSNMTSINLDEYVGLAPDNDQSYRYFMQ
SNLFDKKPFKETFVPNGLAKGPEEETTRYNKVIADHPINIQVLGIGRNGHIGFNEPGSPFDAETRKVPLTQSTIDANARF
FASEDDVPRYAYSMGIGSILKSKKILLLAFGENKADAVKKMIEGPVTNDVPASALQKHSDVVVILDKAAASKLSKK

Specific function: Catalyzes the reversible isomerization-deamination of glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P) and ammonium ion

COG id: COG0363

COG function: function code G; 6-phosphogluconolactonase/Glucosamine-6-phosphate isomerase/deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucosamine/galactosamine-6-phosphate isomerase family. NagB subfamily

Homologues:

Organism=Homo sapiens, GI13027378, Length=251, Percent_Identity=35.8565737051793, Blast_Score=134, Evalue=6e-32,
Organism=Homo sapiens, GI19923881, Length=252, Percent_Identity=34.1269841269841, Blast_Score=124, Evalue=9e-29,
Organism=Escherichia coli, GI1786893, Length=215, Percent_Identity=36.7441860465116, Blast_Score=141, Evalue=3e-35,
Organism=Escherichia coli, GI1789530, Length=206, Percent_Identity=29.6116504854369, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI48994958, Length=206, Percent_Identity=29.6116504854369, Blast_Score=75, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17554876, Length=235, Percent_Identity=35.3191489361702, Blast_Score=133, Evalue=7e-32,
Organism=Drosophila melanogaster, GI24581960, Length=251, Percent_Identity=35.8565737051793, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI19920764, Length=251, Percent_Identity=35.8565737051793, Blast_Score=147, Evalue=7e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAGB_LACC3 (Q033M5)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_808039.1
- ProteinModelPortal:   Q033M5
- SMR:   Q033M5
- STRING:   Q033M5
- GeneID:   4421459
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_2889
- eggNOG:   COG0363
- HOGENOM:   HBG725991
- OMA:   HLSITMG
- PhylomeDB:   Q033M5
- ProtClustDB:   CLSK2318534
- BioCyc:   LCAS321967:LSEI_2889-MONOMER
- HAMAP:   MF_01241
- InterPro:   IPR006148
- InterPro:   IPR004547
- InterPro:   IPR018321
- PANTHER:   PTHR11280
- TIGRFAMs:   TIGR00502

Pfam domain/function: PF01182 Glucosamine_iso

EC number: =3.5.99.6

Molecular weight: Translated: 25825; Mature: 25825

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS01161 GLC_GALNAC_ISOMERASE

Important sites: ACT_SITE 62-62 ACT_SITE 128-128 ACT_SITE 130-130 ACT_SITE 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVKIFDNDTEAGKYAFDLIKQGMDNGAKVLGLATGSTPVTMYKAMVNSDVDFSNMTSIN
CCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCEECC
LDEYVGLAPDNDQSYRYFMQSNLFDKKPFKETFVPNGLAKGPEEETTRYNKVIADHPINI
HHHHCCCCCCCCCHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEE
QVLGIGRNGHIGFNEPGSPFDAETRKVPLTQSTIDANARFFASEDDVPRYAYSMGIGSIL
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
KSKKILLLAFGENKADAVKKMIEGPVTNDVPASALQKHSDVVVILDKAAASKLSKK
CCCCEEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEEEECHHHHHHCCC
>Mature Secondary Structure
MDVKIFDNDTEAGKYAFDLIKQGMDNGAKVLGLATGSTPVTMYKAMVNSDVDFSNMTSIN
CCEEEECCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCCCEECC
LDEYVGLAPDNDQSYRYFMQSNLFDKKPFKETFVPNGLAKGPEEETTRYNKVIADHPINI
HHHHCCCCCCCCCHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEE
QVLGIGRNGHIGFNEPGSPFDAETRKVPLTQSTIDANARFFASEDDVPRYAYSMGIGSIL
EEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHH
KSKKILLLAFGENKADAVKKMIEGPVTNDVPASALQKHSDVVVILDKAAASKLSKK
CCCCEEEEEECCCHHHHHHHHHCCCCCCCCCHHHHHCCCCEEEEEECHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA