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The map label for this gene is malK [H]

Identifier: 116496286

GI number: 116496286

Start: 2850748

End: 2852292

Strand: Direct

Name: malK [H]

Synonym: LSEI_2868

Alternate gene names: 116496286

Gene position: 2850748-2852292 (Clockwise)

Preceding gene: 116496280

Following gene: 116496287

Centisome position: 98.46

GC content: 46.8

Gene sequence:

>1545_bases
ATGAAAATAAAAGGATGGCGGTTATGGGGCTGGCTCTCATTAATTTTTACTTCCACCTTGTTATTAACGGTTCTAACTGT
TTATGGTTTGACCATGCTGGAAAACCGTCAGCAATTTGAAAGCCGTGAAGGTCAATTGTTGATCTCAATTGGCCGTCAGT
TAGCAAAGGATCCCCGTGTAACCGCCGCACTTGAAGCAAACAAGGCTGATTCAGCCACGCGCACCTACGCAGGGACTGTC
GAGAAAAATTTTGATTTAGATTTTGTTGTCATCATGAATATGCAAGGCATTCGCCTGACGCATCCCGATCCCAAACAAAT
CAATCGACATTTTCAAGGTGGTGATGAAGTGACTGCCTTGCGCGGTCATGAGCATGTTTCGGTTCGGCGCGGAACCTTGG
GCCGCTCACTGCGCGGATTCGTCCCAGTGTATCGCCAAGGCAAAGAGATCGGTGTTGTGGCACTAGGCATTAAGGTCACC
TCACTGCAAACACTCATCAAGACCTCGCAAGATGGCTTCCGGCTGGCGCTATTCGCCGGTACGATCATAGGCCTGCTGAC
AGCGTTTTTAGTCACCTACTATATTAAGCATCAGCTTCATGACCTTGAGCCCAAAGAAATTGCTAGGCTGCTTGAAGAAC
GCAATGCAATGCTTGAAGAAACCAAGGACCCTGTCATTGTGATTGATTTGCACCAAGTCATCATGCTCATCAACAAAGCC
GCTGCCAAGCTGCAAAATCACCTTATCACCGAAAGCGCCTTTATTGGCCAGCCATTGACGACCTTGATTGCCAACCCTGA
ACGTGTCAATCTGAATGTCAAAACCGAGCAGTTTTATCAACAGGATGGCCAGGATTATCTTTTCTCTGCCGCACCTATTA
TGGTGAAAAAACAAAAAATAGGTTACGTTATCTTTCTACGAAACGCTACCGAAGCGATCTTTGTCGCCGATCAGTTGGCC
AACACCACCGCCTATGCCAACGCACTGCAAAGCCAGTCACATGAATTCATGAATAAGCTGCACGTGATTTACGGCTTGGT
GGCAATAAAACAATATGACGAATTGGCCATCTACTTGCAAGATTTGTTAAAACCAGAGCAGGAATTTGCAAATCGCTTGG
CCTTGCTGGTCAAAAATCCACTCGTTGCCGGCTTCATGATTGGTGAACGCGAAAAGTTTGCCGAGCGCAAAACGACCCTC
GACTTTGAAATTTCCCCCGAACTACCACCTAATCCTAATACCCAAGAAACCAAAACGCTGTTAACATTAAGCCGCTATCT
ACATTTCGGGCTACTGCAGCACGATTTGCCGGACACCATTTTGATCCAGCTAGAGTACGCACAGAATCGTCTCACGATCG
CCTATCATTTAGACGAACCAGCCAAACGAACGGACTTTATCTCGCTTATTCATCAAGACTACTTCCAACAACTGCTGACG
GAGTTGAACGGCTCAGTCACCGTTAGCTCAACCGATCCGCTGACACTAACATTAACTGTGGACTACCACGAGGAGGCTCA
AAATGACGAACATTCTAATCGTTGA

Upstream 100 bases:

>100_bases
CAGGGGAAGGTTATTTAACTTATTAAACCATAAACCCATTTATTTAAGCGATTGTTTTACTATTCACGAGATGGTTTCTT
TACTGAACAGGAGTATGATC

Downstream 100 bases:

>100_bases
AGATGATCCAATGGTGCAATTTATCCACCGCAATTATTTAGAAAAAATCGGTACTTTTGACACCATCTACTCTTCAGAAA
CCATCGCTGAAGCCAAAAAA

Product: Signal transduction histidine kinase regulating citrate/malate metabolism

Products: NA

Alternate protein names: Malate kinase sensor [H]

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MKIKGWRLWGWLSLIFTSTLLLTVLTVYGLTMLENRQQFESREGQLLISIGRQLAKDPRVTAALEANKADSATRTYAGTV
EKNFDLDFVVIMNMQGIRLTHPDPKQINRHFQGGDEVTALRGHEHVSVRRGTLGRSLRGFVPVYRQGKEIGVVALGIKVT
SLQTLIKTSQDGFRLALFAGTIIGLLTAFLVTYYIKHQLHDLEPKEIARLLEERNAMLEETKDPVIVIDLHQVIMLINKA
AAKLQNHLITESAFIGQPLTTLIANPERVNLNVKTEQFYQQDGQDYLFSAAPIMVKKQKIGYVIFLRNATEAIFVADQLA
NTTAYANALQSQSHEFMNKLHVIYGLVAIKQYDELAIYLQDLLKPEQEFANRLALLVKNPLVAGFMIGEREKFAERKTTL
DFEISPELPPNPNTQETKTLLTLSRYLHFGLLQHDLPDTILIQLEYAQNRLTIAYHLDEPAKRTDFISLIHQDYFQQLLT
ELNGSVTVSSTDPLTLTLTVDYHEEAQNDEHSNR

Sequences:

>Translated_514_residues
MKIKGWRLWGWLSLIFTSTLLLTVLTVYGLTMLENRQQFESREGQLLISIGRQLAKDPRVTAALEANKADSATRTYAGTV
EKNFDLDFVVIMNMQGIRLTHPDPKQINRHFQGGDEVTALRGHEHVSVRRGTLGRSLRGFVPVYRQGKEIGVVALGIKVT
SLQTLIKTSQDGFRLALFAGTIIGLLTAFLVTYYIKHQLHDLEPKEIARLLEERNAMLEETKDPVIVIDLHQVIMLINKA
AAKLQNHLITESAFIGQPLTTLIANPERVNLNVKTEQFYQQDGQDYLFSAAPIMVKKQKIGYVIFLRNATEAIFVADQLA
NTTAYANALQSQSHEFMNKLHVIYGLVAIKQYDELAIYLQDLLKPEQEFANRLALLVKNPLVAGFMIGEREKFAERKTTL
DFEISPELPPNPNTQETKTLLTLSRYLHFGLLQHDLPDTILIQLEYAQNRLTIAYHLDEPAKRTDFISLIHQDYFQQLLT
ELNGSVTVSSTDPLTLTLTVDYHEEAQNDEHSNR
>Mature_514_residues
MKIKGWRLWGWLSLIFTSTLLLTVLTVYGLTMLENRQQFESREGQLLISIGRQLAKDPRVTAALEANKADSATRTYAGTV
EKNFDLDFVVIMNMQGIRLTHPDPKQINRHFQGGDEVTALRGHEHVSVRRGTLGRSLRGFVPVYRQGKEIGVVALGIKVT
SLQTLIKTSQDGFRLALFAGTIIGLLTAFLVTYYIKHQLHDLEPKEIARLLEERNAMLEETKDPVIVIDLHQVIMLINKA
AAKLQNHLITESAFIGQPLTTLIANPERVNLNVKTEQFYQQDGQDYLFSAAPIMVKKQKIGYVIFLRNATEAIFVADQLA
NTTAYANALQSQSHEFMNKLHVIYGLVAIKQYDELAIYLQDLLKPEQEFANRLALLVKNPLVAGFMIGEREKFAERKTTL
DFEISPELPPNPNTQETKTLLTLSRYLHFGLLQHDLPDTILIQLEYAQNRLTIAYHLDEPAKRTDFISLIHQDYFQQLLT
ELNGSVTVSSTDPLTLTLTVDYHEEAQNDEHSNR

Specific function: Member of a two-component regulatory system malK/malR. Involved in the activation of maeA, maeN and yflS in presence of malate. Probably activates malR by phosphorylation [H]

COG id: COG3290

COG function: function code T; Signal transduction histidine kinase regulating citrate/malate metabolism

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790567, Length=472, Percent_Identity=29.8728813559322, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI1786837, Length=365, Percent_Identity=25.7534246575342, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR016120
- InterPro:   IPR005467
- InterPro:   IPR016121 [H]

Pfam domain/function: PF02518 HATPase_c; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 58321; Mature: 58321

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKGWRLWGWLSLIFTSTLLLTVLTVYGLTMLENRQQFESREGQLLISIGRQLAKDPRV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHCCCCCE
TAALEANKADSATRTYAGTVEKNFDLDFVVIMNMQGIRLTHPDPKQINRHFQGGDEVTAL
EEEEECCCCCCCHHEEECCCCCCCCCEEEEEEECCCEEECCCCHHHHHHHCCCCCCEEEE
RGHEHVSVRRGTLGRSLRGFVPVYRQGKEIGVVALGIKVTSLQTLIKTSQDGFRLALFAG
CCCCEEEEECCCCCCHHHCCCHHHHCCCEEEEEEEEEEHHHHHHHHHCCCCCCEEHHHHH
TIIGLLTAFLVTYYIKHQLHDLEPKEIARLLEERNAMLEETKDPVIVIDLHQVIMLINKA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHH
AAKLQNHLITESAFIGQPLTTLIANPERVNLNVKTEQFYQQDGQDYLFSAAPIMVKKQKI
HHHHHHHHHCCHHHCCCCHHHHHCCCCEEEEEEEHHHHHHCCCCCEEEECCCEEEEEECC
GYVIFLRNATEAIFVADQLANTTAYANALQSQSHEFMNKLHVIYGLVAIKQYDELAIYLQ
CEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLLKPEQEFANRLALLVKNPLVAGFMIGEREKFAERKTTLDFEISPELPPNPNTQETKTL
HHCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHCCEEEEECCCCCCCCCCHHHHHH
LTLSRYLHFGLLQHDLPDTILIQLEYAQNRLTIAYHLDEPAKRTDFISLIHQDYFQQLLT
HHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
ELNGSVTVSSTDPLTLTLTVDYHEEAQNDEHSNR
HCCCCEEEECCCCEEEEEEEECCHHCCCCCCCCC
>Mature Secondary Structure
MKIKGWRLWGWLSLIFTSTLLLTVLTVYGLTMLENRQQFESREGQLLISIGRQLAKDPRV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHCCCCCE
TAALEANKADSATRTYAGTVEKNFDLDFVVIMNMQGIRLTHPDPKQINRHFQGGDEVTAL
EEEEECCCCCCCHHEEECCCCCCCCCEEEEEEECCCEEECCCCHHHHHHHCCCCCCEEEE
RGHEHVSVRRGTLGRSLRGFVPVYRQGKEIGVVALGIKVTSLQTLIKTSQDGFRLALFAG
CCCCEEEEECCCCCCHHHCCCHHHHCCCEEEEEEEEEEHHHHHHHHHCCCCCCEEHHHHH
TIIGLLTAFLVTYYIKHQLHDLEPKEIARLLEERNAMLEETKDPVIVIDLHQVIMLINKA
HHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHH
AAKLQNHLITESAFIGQPLTTLIANPERVNLNVKTEQFYQQDGQDYLFSAAPIMVKKQKI
HHHHHHHHHCCHHHCCCCHHHHHCCCCEEEEEEEHHHHHHCCCCCEEEECCCEEEEEECC
GYVIFLRNATEAIFVADQLANTTAYANALQSQSHEFMNKLHVIYGLVAIKQYDELAIYLQ
CEEEEEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLLKPEQEFANRLALLVKNPLVAGFMIGEREKFAERKTTLDFEISPELPPNPNTQETKTL
HHCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHCCEEEEECCCCCCCCCCHHHHHH
LTLSRYLHFGLLQHDLPDTILIQLEYAQNRLTIAYHLDEPAKRTDFISLIHQDYFQQLLT
HHHHHHHHHHHHHCCCCCEEEEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
ELNGSVTVSSTDPLTLTLTVDYHEEAQNDEHSNR
HCCCCEEEECCCCEEEEEEEECCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 12949159; 12949160 [H]