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The map label for this gene is ybjI [H]

Identifier: 116496280

GI number: 116496280

Start: 2843640

End: 2844434

Strand: Direct

Name: ybjI [H]

Synonym: LSEI_2862

Alternate gene names: 116496280

Gene position: 2843640-2844434 (Clockwise)

Preceding gene: 116496279

Following gene: 116496286

Centisome position: 98.22

GC content: 45.16

Gene sequence:

>795_bases
ATGTTCAAATTAATAGCAACAGACATGGATGGCACCTTTTTGAGAGACGACATGACCTATGATCGCGATTTTTTTGCGAC
GTTACATGAAAAAATGCAAGCGCAAGGCATTCAGTGGGTGGTGGCCAGTGGTAATCAATACTTTCAGCTGACTTCCTTTT
TTGAAGCTTTCCCTGATACGATTTATGTTGCTGAAAACGGCGCCTACATTCGCGATGCTAACAAAGTGTATGCTTTAAGT
TCTTTTGAAAACGGTGCCATTCAACCAATTCTGACTAAACTAACTAGCATACCTGATCTCAAAATTCTTGTCTGTGGTCA
ATCAAGCGCCTATGCCCTGAAAACTACTGATGCTGCCCATATCGCAACGGCACGACAATACTACAAGCAATTACGTCTAG
TTGATACTTTCGACAATCTCGATGATCGCATTTTAAAATTTGCCATTAGTTGTCCTCCAGAAAAAACCGCGGCTATTGTT
GCCGAAATGCGCCAACTGTTGGTTGGCTTGGGAGAACCGACAAGCAGCGGCCACGGCGACATTGATATCATTCAGCCCGG
GATGAACAAAGCTGCCGGATTGGCAACACTTGGTCGGCATTTAAACATTCAACTTGCTGATATGTGCGCTTTTGGCGATG
GCGGCAACGATTTAGAAATGCTACGGGAAGTTGGTCTTGGTGTGGCGATGGCAAATGCAACGCCAGCAGTACTGGCAGTC
GCCGACACAACCACTAAGAGCAATCAGGAACAAGGTGTCTTGCAGTTTATGCATCATCTGTTATCTGAACCCTGA

Upstream 100 bases:

>100_bases
CAGTGGCAACAACAAAAAGATGAGTTCTTAACCTGGCAGCAACAGGCTGATGAAAGCCAACAATCAAAGCAGTCTTAACT
GACCATTAACGGAGAGAACA

Downstream 100 bases:

>100_bases
AGCCTTGTTGGAAGAAGCCACTAAAAAGGAGGCCGCATGCATCTCGATTTTGCGATGCATGCGGCCTCCTTTTTTGTGTT
CCACGTGAAACTCACTCGAA

Product: HAD superfamily hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MFKLIATDMDGTFLRDDMTYDRDFFATLHEKMQAQGIQWVVASGNQYFQLTSFFEAFPDTIYVAENGAYIRDANKVYALS
SFENGAIQPILTKLTSIPDLKILVCGQSSAYALKTTDAAHIATARQYYKQLRLVDTFDNLDDRILKFAISCPPEKTAAIV
AEMRQLLVGLGEPTSSGHGDIDIIQPGMNKAAGLATLGRHLNIQLADMCAFGDGGNDLEMLREVGLGVAMANATPAVLAV
ADTTTKSNQEQGVLQFMHHLLSEP

Sequences:

>Translated_264_residues
MFKLIATDMDGTFLRDDMTYDRDFFATLHEKMQAQGIQWVVASGNQYFQLTSFFEAFPDTIYVAENGAYIRDANKVYALS
SFENGAIQPILTKLTSIPDLKILVCGQSSAYALKTTDAAHIATARQYYKQLRLVDTFDNLDDRILKFAISCPPEKTAAIV
AEMRQLLVGLGEPTSSGHGDIDIIQPGMNKAAGLATLGRHLNIQLADMCAFGDGGNDLEMLREVGLGVAMANATPAVLAV
ADTTTKSNQEQGVLQFMHHLLSEP
>Mature_264_residues
MFKLIATDMDGTFLRDDMTYDRDFFATLHEKMQAQGIQWVVASGNQYFQLTSFFEAFPDTIYVAENGAYIRDANKVYALS
SFENGAIQPILTKLTSIPDLKILVCGQSSAYALKTTDAAHIATARQYYKQLRLVDTFDNLDDRILKFAISCPPEKTAAIV
AEMRQLLVGLGEPTSSGHGDIDIIQPGMNKAAGLATLGRHLNIQLADMCAFGDGGNDLEMLREVGLGVAMANATPAVLAV
ADTTTKSNQEQGVLQFMHHLLSEP

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates FMN and beta-glucose 1-phosphate [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081790, Length=261, Percent_Identity=40.9961685823755, Blast_Score=204, Evalue=4e-54,
Organism=Escherichia coli, GI1787043, Length=260, Percent_Identity=34.6153846153846, Blast_Score=171, Evalue=5e-44,
Organism=Escherichia coli, GI1786982, Length=276, Percent_Identity=25, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 28863; Mature: 28863

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKLIATDMDGTFLRDDMTYDRDFFATLHEKMQAQGIQWVVASGNQYFQLTSFFEAFPDT
CEEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEHHHHHHHCCCE
IYVAENGAYIRDANKVYALSSFENGAIQPILTKLTSIPDLKILVCGQSSAYALKTTDAAH
EEEECCCCEEEECCEEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCCCEEEECCCHHH
IATARQYYKQLRLVDTFDNLDDRILKFAISCPPEKTAAIVAEMRQLLVGLGEPTSSGHGD
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
IDIIQPGMNKAAGLATLGRHLNIQLADMCAFGDGGNDLEMLREVGLGVAMANATPAVLAV
EEEECCCCCHHHHHHHHHHHCCEEEEHEEEECCCCCHHHHHHHHCCCEEECCCCCEEEEE
ADTTTKSNQEQGVLQFMHHLLSEP
ECCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFKLIATDMDGTFLRDDMTYDRDFFATLHEKMQAQGIQWVVASGNQYFQLTSFFEAFPDT
CEEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCEEEHHHHHHHCCCE
IYVAENGAYIRDANKVYALSSFENGAIQPILTKLTSIPDLKILVCGQSSAYALKTTDAAH
EEEECCCCEEEECCEEEEEECCCCCCHHHHHHHHCCCCCEEEEEECCCCCEEEECCCHHH
IATARQYYKQLRLVDTFDNLDDRILKFAISCPPEKTAAIVAEMRQLLVGLGEPTSSGHGD
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
IDIIQPGMNKAAGLATLGRHLNIQLADMCAFGDGGNDLEMLREVGLGVAMANATPAVLAV
EEEECCCCCHHHHHHHHHHHCCEEEEHEEEECCCCCHHHHHHHHCCCEEECCCCCEEEEE
ADTTTKSNQEQGVLQFMHHLLSEP
ECCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]