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The map label for this gene is ansA [H]

Identifier: 116495705

GI number: 116495705

Start: 2248188

End: 2249162

Strand: Direct

Name: ansA [H]

Synonym: LSEI_2253

Alternate gene names: 116495705

Gene position: 2248188-2249162 (Clockwise)

Preceding gene: 116495698

Following gene: 116495720

Centisome position: 77.65

GC content: 50.77

Gene sequence:

>975_bases
GTGACTAAACATATTCTCGTTTTGCATACGGGTGGTACCATTTCGATGACTGAAAATAGCGAAGGTGACATTATGCCCAG
CCAACATAACCCGTTACTTGGTGCTGACAGTTTTGTCCCTGCTGACGTCGAACTCACCACTGAAGAAATTTTCAACCTCC
CATCCCCGCACATGACCCCAGAACGGATGCTAGAACTAGTTAAACGCATCCGCCAAGCGGAAAAAGAAGGCAGCGATGGC
GTCGTGATCACTCACGGGACGGATACGCTGGAAGAAACCGCCTATTTTCTTGATCTGACCCTTGATTCGGCGATGCCAGT
GGTCGTGACAGGCGCTATGCGCTCTAGTAACGAAATCGGCAGCGATGGTCTGGAGAACCTTCAAAGCGCCATTAAAACGG
CTGCCAGCGACGACTCACGCAATAAGGGAACGCTCGTTGTCATGAATGACGAAATTCATACCGCTCGGTTCGTGACCAAG
ACGCACACGACCAACGTGGCTACTTTCCGAACACCAACATTCGGTCCAGTCGGTTTGGTCACCAAAGATGGCGTCCGATT
CTTTCAGGAGTTGATTAATCAGGAAGTCTGTGCGATTGATCACCTCGTCGACAAGGTCTTCCTCATCAAAGCATATGCTG
GCATGGATGGTACCTTGTTCGATGCACTGCCTGAAGATACGCACGGCTTGACGATTGAAGCGCTCGGTGCCGGCAATCTA
CCGCCGACCACCTTACCTGCCATTCAACGGCTGCTGGATCGCAACATCCCGATTGTTCTCGTTTCCCGCTGTTTTAATGG
TGTTGCGCAAGCCGTTTACAATTACGAAGGCGGTGGTGTGCAACTTAAGAAAATGGGCATCATTTTCTGTCAAGGCTTGA
ATGGCCAAAAGGCACGAATCAAGTTGCAAGTCGGCCTCAGTGACGGCAAACAAGGGCAGGATCTAGCCGACTTCGTGGAT
AATGCTGTTAGCTGA

Upstream 100 bases:

>100_bases
CACCTAATTATGGGTGTTCGCGCCATTGACTTTAGCTGTTCGCGGCTGCATAAAGAGCCGTATAATGAAATGCGACAACG
TTTGAAAGGAAGATATGACC

Downstream 100 bases:

>100_bases
CAATTAACAAATAGCTGGGATTATCCTCGATTTCGAAGACCGCAACGCAAAAACGGCACTAAGCAAAAAAAGAGACGTTT
GCTTAGTGCCGTTTTACTAT

Product: L-asparaginase

Products: NA

Alternate protein names: L-ASNase; L-asparagine amidohydrolase [H]

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MTKHILVLHTGGTISMTENSEGDIMPSQHNPLLGADSFVPADVELTTEEIFNLPSPHMTPERMLELVKRIRQAEKEGSDG
VVITHGTDTLEETAYFLDLTLDSAMPVVVTGAMRSSNEIGSDGLENLQSAIKTAASDDSRNKGTLVVMNDEIHTARFVTK
THTTNVATFRTPTFGPVGLVTKDGVRFFQELINQEVCAIDHLVDKVFLIKAYAGMDGTLFDALPEDTHGLTIEALGAGNL
PPTTLPAIQRLLDRNIPIVLVSRCFNGVAQAVYNYEGGGVQLKKMGIIFCQGLNGQKARIKLQVGLSDGKQGQDLADFVD
NAVS

Sequences:

>Translated_324_residues
MTKHILVLHTGGTISMTENSEGDIMPSQHNPLLGADSFVPADVELTTEEIFNLPSPHMTPERMLELVKRIRQAEKEGSDG
VVITHGTDTLEETAYFLDLTLDSAMPVVVTGAMRSSNEIGSDGLENLQSAIKTAASDDSRNKGTLVVMNDEIHTARFVTK
THTTNVATFRTPTFGPVGLVTKDGVRFFQELINQEVCAIDHLVDKVFLIKAYAGMDGTLFDALPEDTHGLTIEALGAGNL
PPTTLPAIQRLLDRNIPIVLVSRCFNGVAQAVYNYEGGGVQLKKMGIIFCQGLNGQKARIKLQVGLSDGKQGQDLADFVD
NAVS
>Mature_323_residues
TKHILVLHTGGTISMTENSEGDIMPSQHNPLLGADSFVPADVELTTEEIFNLPSPHMTPERMLELVKRIRQAEKEGSDGV
VITHGTDTLEETAYFLDLTLDSAMPVVVTGAMRSSNEIGSDGLENLQSAIKTAASDDSRNKGTLVVMNDEIHTARFVTKT
HTTNVATFRTPTFGPVGLVTKDGVRFFQELINQEVCAIDHLVDKVFLIKAYAGMDGTLFDALPEDTHGLTIEALGAGNLP
PTTLPAIQRLLDRNIPIVLVSRCFNGVAQAVYNYEGGGVQLKKMGIIFCQGLNGQKARIKLQVGLSDGKQGQDLADFVDN
AVS

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1789327, Length=281, Percent_Identity=35.5871886120996, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1788065, Length=281, Percent_Identity=29.5373665480427, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI193207374, Length=350, Percent_Identity=24.5714285714286, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI193207372, Length=350, Percent_Identity=24.5714285714286, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17558278, Length=350, Percent_Identity=24.5714285714286, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17558280, Length=350, Percent_Identity=24.5714285714286, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323189, Length=324, Percent_Identity=30.2469135802469, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323187, Length=324, Percent_Identity=30.2469135802469, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323186, Length=324, Percent_Identity=30.2469135802469, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6323184, Length=324, Percent_Identity=30.2469135802469, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320527, Length=317, Percent_Identity=27.1293375394322, Blast_Score=113, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24645530, Length=238, Percent_Identity=28.9915966386555, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 35006; Mature: 34875

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKHILVLHTGGTISMTENSEGDIMPSQHNPLLGADSFVPADVELTTEEIFNLPSPHMTP
CCCEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEECHHHHHCCCCCCCCH
ERMLELVKRIRQAEKEGSDGVVITHGTDTLEETAYFLDLTLDSAMPVVVTGAMRSSNEIG
HHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHEEEEEEEECCCCCEEEEECCCCCCCCC
SDGLENLQSAIKTAASDDSRNKGTLVVMNDEIHTARFVTKTHTTNVATFRTPTFGPVGLV
CHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEEEEECCCCEEEEECCCCCCCCEE
TKDGVRFFQELINQEVCAIDHLVDKVFLIKAYAGMDGTLFDALPEDTHGLTIEALGAGNL
EHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCEEEEEECCCCC
PPTTLPAIQRLLDRNIPIVLVSRCFNGVAQAVYNYEGGGVQLKKMGIIFCQGLNGQKARI
CCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCEEEE
KLQVGLSDGKQGQDLADFVDNAVS
EEEEECCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
TKHILVLHTGGTISMTENSEGDIMPSQHNPLLGADSFVPADVELTTEEIFNLPSPHMTP
CCEEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCEECHHHHHCCCCCCCCH
ERMLELVKRIRQAEKEGSDGVVITHGTDTLEETAYFLDLTLDSAMPVVVTGAMRSSNEIG
HHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHEEEEEEEECCCCCEEEEECCCCCCCCC
SDGLENLQSAIKTAASDDSRNKGTLVVMNDEIHTARFVTKTHTTNVATFRTPTFGPVGLV
CHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEEEEEECCCCEEEEECCCCCCCCEE
TKDGVRFFQELINQEVCAIDHLVDKVFLIKAYAGMDGTLFDALPEDTHGLTIEALGAGNL
EHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCEEEEEECCCCC
PPTTLPAIQRLLDRNIPIVLVSRCFNGVAQAVYNYEGGGVQLKKMGIIFCQGLNGQKARI
CCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCEEEE
KLQVGLSDGKQGQDLADFVDNAVS
EEEEECCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1916233 [H]