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The map label for this gene is yxjB [H]

Identifier: 116494420

GI number: 116494420

Start: 888671

End: 889513

Strand: Direct

Name: yxjB [H]

Synonym: LSEI_0913

Alternate gene names: 116494420

Gene position: 888671-889513 (Clockwise)

Preceding gene: 116494417

Following gene: 116494421

Centisome position: 30.69

GC content: 51.25

Gene sequence:

>843_bases
ATGCGTAAGCGTGAAACAAAAATTCATTTGGCAGCAGCACAAGCACCGCTTTTTGCTTGTCCAGTCTGTGGCCAACCATT
ACAGGTGACAGCGGGATCGTTGGTTTGTGTCAACGGACATGTACGCGATTTCAGCCACAAAGGGACGCTGAACTTCCTGA
ATCAGCAAGTAGCCACCGAATACACGACGCCGATGTTAGCCGCACGACGGCGAATGTTGAACGCTGGTTTGTTCACCCCA
TTTTTGGCAGCTATGGCAGCCAAGCTTCCGAAACATCAACGACTGTTAGATGTCGGCTGCGGGGAAGGCACGCCGACTGC
GTATTTGGCCGAGGCGGGTCAACAAACTGCCGTGGGTTTTGATATCTCATCACCGGCGATCAATCTAGCAGGCGGCTTAG
CGGCACCAGTTCTGTTTGCGGTTGCGGACCTGGCCCACCTGCCTTTCGTTAACGATGCATTTGATACCGTGACCGACATC
TTTTCGCCAGGTAATTATCGTGAATTCAGACGTGTGCTGCGTCCTGATGGTCAGCTATTGAAAATTATTCCGCGGGCGGG
TTATCTTCGAGAAATCCGGGAAGGCTTATACTCCGGGACGGCTAAGGCTGTGTATAACAATGAGCCTGTCTTAAAAAGGT
TCTTAGCAGCTTTCCCTGATGCGAGCCAGGAGACAATCACCTACGATTTTCCGTTAGCGCCAACTCAATTTGGTGATTTA
ATGGCCATGACCCCGTTAACGTGGCAGGCGCCCGCCGATCAACGCGACCAAATGCTTGCTGCACCGCCGATTAGTATCCA
TATTGATGTTGATTTGCTCACTGTGTCGCATCTTGTCAAGTAA

Upstream 100 bases:

>100_bases
CCGGTTTTGTCATCAAATCGATAAATTGCAAGCATAGCGGGCTCCTTGGCTTTTTCTTTTAGTTTACAACAAGCAACAGC
AGGATGCAGGAGGGAAACAC

Downstream 100 bases:

>100_bases
AAGGGTTGTAAACATCGATGGCTGGTGTTAATATTCCTTTAAGCTATTTATTTTTAACTTTTATCAGGGCTAGCTTTTGG
GTAATTGATAAAAGTTTGCG

Product: SAM-dependent methyltransferase

Products: S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MRKRETKIHLAAAQAPLFACPVCGQPLQVTAGSLVCVNGHVRDFSHKGTLNFLNQQVATEYTTPMLAARRRMLNAGLFTP
FLAAMAAKLPKHQRLLDVGCGEGTPTAYLAEAGQQTAVGFDISSPAINLAGGLAAPVLFAVADLAHLPFVNDAFDTVTDI
FSPGNYREFRRVLRPDGQLLKIIPRAGYLREIREGLYSGTAKAVYNNEPVLKRFLAAFPDASQETITYDFPLAPTQFGDL
MAMTPLTWQAPADQRDQMLAAPPISIHIDVDLLTVSHLVK

Sequences:

>Translated_280_residues
MRKRETKIHLAAAQAPLFACPVCGQPLQVTAGSLVCVNGHVRDFSHKGTLNFLNQQVATEYTTPMLAARRRMLNAGLFTP
FLAAMAAKLPKHQRLLDVGCGEGTPTAYLAEAGQQTAVGFDISSPAINLAGGLAAPVLFAVADLAHLPFVNDAFDTVTDI
FSPGNYREFRRVLRPDGQLLKIIPRAGYLREIREGLYSGTAKAVYNNEPVLKRFLAAFPDASQETITYDFPLAPTQFGDL
MAMTPLTWQAPADQRDQMLAAPPISIHIDVDLLTVSHLVK
>Mature_280_residues
MRKRETKIHLAAAQAPLFACPVCGQPLQVTAGSLVCVNGHVRDFSHKGTLNFLNQQVATEYTTPMLAARRRMLNAGLFTP
FLAAMAAKLPKHQRLLDVGCGEGTPTAYLAEAGQQTAVGFDISSPAINLAGGLAAPVLFAVADLAHLPFVNDAFDTVTDI
FSPGNYREFRRVLRPDGQLLKIIPRAGYLREIREGLYSGTAKAVYNNEPVLKRFLAAFPDASQETITYDFPLAPTQFGDL
MAMTPLTWQAPADQRDQMLAAPPISIHIDVDLLTVSHLVK

Specific function: Specifically Methylates The Guanosine In Position 745 Of 23s rRNA. [C]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmA family [H]

Homologues:

Organism=Escherichia coli, GI1788125, Length=265, Percent_Identity=29.0566037735849, Blast_Score=109, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR016718 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: 2.1.1.51 [C]

Molecular weight: Translated: 30443; Mature: 30443

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKRETKIHLAAAQAPLFACPVCGQPLQVTAGSLVCVNGHVRDFSHKGTLNFLNQQVATE
CCCCCCEEEEEECCCCEEECCCCCCCCEECCCCEEEEECCEECCCCCCHHHHHHHHHHHH
YTTPMLAARRRMLNAGLFTPFLAAMAAKLPKHQRLLDVGCGEGTPTAYLAEAGQQTAVGF
HHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHCCCCEEEEE
DISSPAINLAGGLAAPVLFAVADLAHLPFVNDAFDTVTDIFSPGNYREFRRVLRPDGQLL
ECCCCCHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCEE
KIIPRAGYLREIREGLYSGTAKAVYNNEPVLKRFLAAFPDASQETITYDFPLAPTQFGDL
EEECCCHHHHHHHHHHHCCCHHEEECCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCC
MAMTPLTWQAPADQRDQMLAAPPISIHIDVDLLTVSHLVK
CEECCCCCCCCCCCCCCEEECCCEEEEEEEHHHHHHHHCC
>Mature Secondary Structure
MRKRETKIHLAAAQAPLFACPVCGQPLQVTAGSLVCVNGHVRDFSHKGTLNFLNQQVATE
CCCCCCEEEEEECCCCEEECCCCCCCCEECCCCEEEEECCEECCCCCCHHHHHHHHHHHH
YTTPMLAARRRMLNAGLFTPFLAAMAAKLPKHQRLLDVGCGEGTPTAYLAEAGQQTAVGF
HHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHCCCCEEEEE
DISSPAINLAGGLAAPVLFAVADLAHLPFVNDAFDTVTDIFSPGNYREFRRVLRPDGQLL
ECCCCCHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCEE
KIIPRAGYLREIREGLYSGTAKAVYNNEPVLKRFLAAFPDASQETITYDFPLAPTQFGDL
EEECCCHHHHHHHHHHHCCCHHEEECCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCC
MAMTPLTWQAPADQRDQMLAAPPISIHIDVDLLTVSHLVK
CEECCCCCCCCCCCCCCEEECCCEEEEEEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Unidentified protein factor B [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; rRNA [C]

Specific reaction: S-Adenosyl-L-methionine + rRNA = S-adenosyl-L-homocysteine +rRNA containing Nmethylguanine [C]

General reaction: Methyl group transfer [C]

Inhibitor: Mg2+; Unidentified protein factor A1 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969509; 9384377 [H]