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The map label for this gene is yibK [C]

Identifier: 116494421

GI number: 116494421

Start: 889767

End: 890276

Strand: Direct

Name: yibK [C]

Synonym: LSEI_0914

Alternate gene names: 116494421

Gene position: 889767-890276 (Clockwise)

Preceding gene: 116494420

Following gene: 116494422

Centisome position: 30.73

GC content: 44.71

Gene sequence:

>510_bases
ATGGCGAATCACATTGTTTTATTTGAACCGCTGATTCCGGCAAATACCGGGAACATTGCCAGAACCTGTGCAGCAACGAA
TAGCTATTTGCATCTCATCAAGCCACTGGGATTTGAGACAGATGATAAGCATCTGCGTCGTGCAGGTCTGGATTATTGGC
AACGCGTCCGGATCATCTATCATGATAGTCTGGATGACTTCATGGCAACCGTTAAGGAACCCCAATACCTTTACCTTATT
ACTAAGTTTGCTGATCAGACATACTCAGACCGAGATTATGCCGACATTGATCAGGATCATTATTTCCTTTTTGGCAAAGA
GACAACGGGACTACCGGAAGGCTTCATGCGCGATCATGCCGATCAGTGCTTACGTATTCCCATGACTGACAACGTCCGCG
CATTGAATTTGTCAAATTGCGCAGCATTGGTCATTTATGAAGCTTTACGCCAGCAGCATTTTGCAGGACTTGAAACATCA
CATACGTACGAGCACGACAAATTAAAGTAA

Upstream 100 bases:

>100_bases
TTTTTTTTTGCTTATTTTTACAAATTGACCTGTCCACAAGGCCTGTTCGTGCAAGTTAAATGTGCTAAACTTAAACCACT
TGCGTTGAAGGGAAGATAAC

Downstream 100 bases:

>100_bases
CCCAACAATAGAAGTGTGCGAGCTGGCTCGAGTAGAAACTGGCATGTGAATGGCTTTGACCGTTATGGAAAAGGACGCTT
ACAAGAAGTTTTCTCGGTTG

Product: rRNA methylase (SpoU class)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MANHIVLFEPLIPANTGNIARTCAATNSYLHLIKPLGFETDDKHLRRAGLDYWQRVRIIYHDSLDDFMATVKEPQYLYLI
TKFADQTYSDRDYADIDQDHYFLFGKETTGLPEGFMRDHADQCLRIPMTDNVRALNLSNCAALVIYEALRQQHFAGLETS
HTYEHDKLK

Sequences:

>Translated_169_residues
MANHIVLFEPLIPANTGNIARTCAATNSYLHLIKPLGFETDDKHLRRAGLDYWQRVRIIYHDSLDDFMATVKEPQYLYLI
TKFADQTYSDRDYADIDQDHYFLFGKETTGLPEGFMRDHADQCLRIPMTDNVRALNLSNCAALVIYEALRQQHFAGLETS
HTYEHDKLK
>Mature_168_residues
ANHIVLFEPLIPANTGNIARTCAATNSYLHLIKPLGFETDDKHLRRAGLDYWQRVRIIYHDSLDDFMATVKEPQYLYLIT
KFADQTYSDRDYADIDQDHYFLFGKETTGLPEGFMRDHADQCLRIPMTDNVRALNLSNCAALVIYEALRQQHFAGLETSH
TYEHDKLK

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=154, Percent_Identity=44.8051948051948, Blast_Score=128, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 19585; Mature: 19454

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANHIVLFEPLIPANTGNIARTCAATNSYLHLIKPLGFETDDKHLRRAGLDYWQRVRIIY
CCCCEEEEECCCCCCCCCHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEE
HDSLDDFMATVKEPQYLYLITKFADQTYSDRDYADIDQDHYFLFGKETTGLPEGFMRDHA
ECCHHHHHHHHCCCCEEEEEEEHHCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHH
DQCLRIPMTDNVRALNLSNCAALVIYEALRQQHFAGLETSHTYEHDKLK
HHEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
ANHIVLFEPLIPANTGNIARTCAATNSYLHLIKPLGFETDDKHLRRAGLDYWQRVRIIY
CCCEEEEECCCCCCCCCHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHEE
HDSLDDFMATVKEPQYLYLITKFADQTYSDRDYADIDQDHYFLFGKETTGLPEGFMRDHA
ECCHHHHHHHHCCCCEEEEEEEHHCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHH
DQCLRIPMTDNVRALNLSNCAALVIYEALRQQHFAGLETSHTYEHDKLK
HHEEECCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA