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The map label for this gene is leuS

Identifier: 116494392

GI number: 116494392

Start: 859077

End: 861488

Strand: Direct

Name: leuS

Synonym: LSEI_0883

Alternate gene names: 116494392

Gene position: 859077-861488 (Clockwise)

Preceding gene: 116494390

Following gene: 116494393

Centisome position: 29.67

GC content: 47.18

Gene sequence:

>2412_bases
ATGGCATACGATCATCATGAGATCGATAAGAAGTGGCAGCGTTATTGGGCCGAGCATAACGAATTTAATACCACAACCGA
TCCTAAGAAGCCGAATTATTATGCGTTGGACATGTTTCCTTATCCGTCAGGCCAAGGCCTGCATGTTGGGCATCCGGAAG
GGTATACGGCAACTGATATTGTTGCACGCATGAAACGGATGCAAGGGTTCAATGTTCTTCATCCTATGGGCTGGGATGCA
TTCGGCTTGCCAGCTGAGCAATATGCCTTGAACACGGGTCACAATCCGAAGACCTTTACCAAACAAAATATTGAGACCTT
TAAGCGTCAAATCAACAGCCTTGGCTTTTCATATGACTGGAATCGTGAAATCAACACAACGGATCCGAATTACTACAAGT
GGACCCAGTGGATTTTTGAACAACTTTATAAACATGGTCTGGCATATGAAGCTGAGGTACCGGTTAACTGGAGTCCGGAT
CTAGGGACGGTTGTTGCAAACGAAGAAGTGATTGATGGCAAAACGGAGCGTGGCGGGTTCCCAGTTGTTCGGAAGCCGAT
GCGTCAGTGGATGCTGAAAATCACAGCCTATGCTGAAAAGCTGTTGACGGATCTGGACGATCTTGACTGGCCGGAATCAA
TTAAGCAGATGCAACGCAACTGGATTGGCAAATCAACTGGGGCACAGATCACCTTCCGGGTAACTGACAGTCATGAGCCG
TTTGATGTCTTTACCACTCGTCCAGATACGCTGTTTGGTGCAACGTATGTCGTGATGGCACCAGAACATGAATTAGTTCA
GAAAATTACCACCCCGGCACAACAGGCCGTCGTTGATGCGTACATTGACGAAGCCGCCCATAAGTCAGATCTTGATCGTA
CCGCTTTAGATAAGGAAAAGACTGGTGTTTGGACCGGGGCCTACGCGACTAATCCGGTTAACGGCGAAAAACTGCCGATT
TGGATTTCAGACTACGTTTTAGCCTCTTATGGGACTGGCGCAATCATGTCCGTGCCTGCGCATGATGATCGTGATTACGC
TTTTGCCAAAAAGTTTGGTATTGAAATCAAGCCTGTTATTGAAGGCGGCAATGTCGATGAAGCAGCTTATACTGGCGATG
GCGTGCACATCAACTCCGGGTTCCTTGATGGTTTGAATGAACACGACGCGATTGATCGAATGATCAAATGGCTTGAAGAT
AAAGGTATCGGCAGTGCCAAAATCAATTACAAACTGCGTGATTGGGTCTTTTCACGCCAGCGTTATTGGGGTGAACCGAT
TCCTGTCATTCACTGGGAAGATGGCGAAACCACGTTGGTGCCAGAAGACGAACTGCCCCTCACATTACCTGAAGAGGCTG
ATATCAAGCCAAGCGGTACTGGCGAGAGTCCACTGGCAAATCTGACTGACTGGGTTAATGTGGTCGACAAGAACGGGCGG
AAAGGTAAGCGTGAGACCAATACAATGCCGCAATGGGCAGGGTCTTCATGGTACTTCCTGCGGTTTGTTGATCCTCACAA
CAAGGAAGCTCTTGCGGATTACGACAAACTTAAGGCATGGATGCCAGTTGATCTGTACATTGGAGGGGCTGAACATGCTG
TGCTGCACCTGCTGTATGCCCGTTTCTGGAATCTGTTCCTTTATGACATCGGGGCGATCCCGAACAAAGAACCGTTCCAA
CGGCTATTTAACCAAGGGATGATTCTGGGCGATAATCATGAAAAGATGAGTAAGTCCAAGGGCAATGTGGTTAATCCAGA
TGATGTGGTTGATGAGTATGGTGCCGATACCTTGCGGCTGTACGAAATGTTTATGGGGCCGCTGGATGCTGGTATTGCTT
GGTCAACAAAAGGGTTAGCTGGTGCCCGCAAGTTCTTGGATCGCGTCTGGAGTGCTTTCATTGATGATGAAGGCAAACTG
CGTGATCGGATTACGACGATCAATGACGGTCGACTTGATAAGGTTTACAATGAGACGGTCAAGAAGGTTACCGAGGACTA
CGATGCTTTGCATTTCAACACGGCAATTTCACAGATGATGGTCTTCATCAACTCAGCGCGTAAAGATGATGACCTGCCAC
TAGAATATGTTGAAGGATTCGTGAAAATGCTTGCGCCGATTGCCCCGCATCTAATGGAAGAAATTTGGTCACGGCTAGGT
CACGATCATAGTCTGACTTATGCGCCATGGCCAAGTTATGATGAAAGCAAAATCAAAACCGACACTTACGATATGATGAT
CCAAGTCAATGGCAAGCTGCGCGGTTCGATTACGGCGGATGTCAACGAATCAGATGATGAGATCAAACAAGCGGCTTTGG
CAAATGATAATGTTCAGAAATTTACGGCGGGCAAAGACATTAAGAAAATTATTGTCGTACCGCGAAAAATCGTGAATATT
GTCGCAAAATAA

Upstream 100 bases:

>100_bases
TTTGAGCGCTGTGAAAGCAGAACTTAGGTGGTACCGCGGCTGAGCCGTCCTAATGTAGATAGGGCGGCTTTTTGTTTGAA
ATTAAGTGGGAGGTAACAAA

Downstream 100 bases:

>100_bases
CCGCACTGGCTTCACCAATTAACTGAAAGAAAAAATGGTCAACGAATGGTTCCTGAAGGAAGCGGGCGTTGACCATTTTT
TTAGCGATTCAGCTGCATGA

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS

Number of amino acids: Translated: 803; Mature: 802

Protein sequence:

>803_residues
MAYDHHEIDKKWQRYWAEHNEFNTTTDPKKPNYYALDMFPYPSGQGLHVGHPEGYTATDIVARMKRMQGFNVLHPMGWDA
FGLPAEQYALNTGHNPKTFTKQNIETFKRQINSLGFSYDWNREINTTDPNYYKWTQWIFEQLYKHGLAYEAEVPVNWSPD
LGTVVANEEVIDGKTERGGFPVVRKPMRQWMLKITAYAEKLLTDLDDLDWPESIKQMQRNWIGKSTGAQITFRVTDSHEP
FDVFTTRPDTLFGATYVVMAPEHELVQKITTPAQQAVVDAYIDEAAHKSDLDRTALDKEKTGVWTGAYATNPVNGEKLPI
WISDYVLASYGTGAIMSVPAHDDRDYAFAKKFGIEIKPVIEGGNVDEAAYTGDGVHINSGFLDGLNEHDAIDRMIKWLED
KGIGSAKINYKLRDWVFSRQRYWGEPIPVIHWEDGETTLVPEDELPLTLPEEADIKPSGTGESPLANLTDWVNVVDKNGR
KGKRETNTMPQWAGSSWYFLRFVDPHNKEALADYDKLKAWMPVDLYIGGAEHAVLHLLYARFWNLFLYDIGAIPNKEPFQ
RLFNQGMILGDNHEKMSKSKGNVVNPDDVVDEYGADTLRLYEMFMGPLDAGIAWSTKGLAGARKFLDRVWSAFIDDEGKL
RDRITTINDGRLDKVYNETVKKVTEDYDALHFNTAISQMMVFINSARKDDDLPLEYVEGFVKMLAPIAPHLMEEIWSRLG
HDHSLTYAPWPSYDESKIKTDTYDMMIQVNGKLRGSITADVNESDDEIKQAALANDNVQKFTAGKDIKKIIVVPRKIVNI
VAK

Sequences:

>Translated_803_residues
MAYDHHEIDKKWQRYWAEHNEFNTTTDPKKPNYYALDMFPYPSGQGLHVGHPEGYTATDIVARMKRMQGFNVLHPMGWDA
FGLPAEQYALNTGHNPKTFTKQNIETFKRQINSLGFSYDWNREINTTDPNYYKWTQWIFEQLYKHGLAYEAEVPVNWSPD
LGTVVANEEVIDGKTERGGFPVVRKPMRQWMLKITAYAEKLLTDLDDLDWPESIKQMQRNWIGKSTGAQITFRVTDSHEP
FDVFTTRPDTLFGATYVVMAPEHELVQKITTPAQQAVVDAYIDEAAHKSDLDRTALDKEKTGVWTGAYATNPVNGEKLPI
WISDYVLASYGTGAIMSVPAHDDRDYAFAKKFGIEIKPVIEGGNVDEAAYTGDGVHINSGFLDGLNEHDAIDRMIKWLED
KGIGSAKINYKLRDWVFSRQRYWGEPIPVIHWEDGETTLVPEDELPLTLPEEADIKPSGTGESPLANLTDWVNVVDKNGR
KGKRETNTMPQWAGSSWYFLRFVDPHNKEALADYDKLKAWMPVDLYIGGAEHAVLHLLYARFWNLFLYDIGAIPNKEPFQ
RLFNQGMILGDNHEKMSKSKGNVVNPDDVVDEYGADTLRLYEMFMGPLDAGIAWSTKGLAGARKFLDRVWSAFIDDEGKL
RDRITTINDGRLDKVYNETVKKVTEDYDALHFNTAISQMMVFINSARKDDDLPLEYVEGFVKMLAPIAPHLMEEIWSRLG
HDHSLTYAPWPSYDESKIKTDTYDMMIQVNGKLRGSITADVNESDDEIKQAALANDNVQKFTAGKDIKKIIVVPRKIVNI
VAK
>Mature_802_residues
AYDHHEIDKKWQRYWAEHNEFNTTTDPKKPNYYALDMFPYPSGQGLHVGHPEGYTATDIVARMKRMQGFNVLHPMGWDAF
GLPAEQYALNTGHNPKTFTKQNIETFKRQINSLGFSYDWNREINTTDPNYYKWTQWIFEQLYKHGLAYEAEVPVNWSPDL
GTVVANEEVIDGKTERGGFPVVRKPMRQWMLKITAYAEKLLTDLDDLDWPESIKQMQRNWIGKSTGAQITFRVTDSHEPF
DVFTTRPDTLFGATYVVMAPEHELVQKITTPAQQAVVDAYIDEAAHKSDLDRTALDKEKTGVWTGAYATNPVNGEKLPIW
ISDYVLASYGTGAIMSVPAHDDRDYAFAKKFGIEIKPVIEGGNVDEAAYTGDGVHINSGFLDGLNEHDAIDRMIKWLEDK
GIGSAKINYKLRDWVFSRQRYWGEPIPVIHWEDGETTLVPEDELPLTLPEEADIKPSGTGESPLANLTDWVNVVDKNGRK
GKRETNTMPQWAGSSWYFLRFVDPHNKEALADYDKLKAWMPVDLYIGGAEHAVLHLLYARFWNLFLYDIGAIPNKEPFQR
LFNQGMILGDNHEKMSKSKGNVVNPDDVVDEYGADTLRLYEMFMGPLDAGIAWSTKGLAGARKFLDRVWSAFIDDEGKLR
DRITTINDGRLDKVYNETVKKVTEDYDALHFNTAISQMMVFINSARKDDDLPLEYVEGFVKMLAPIAPHLMEEIWSRLGH
DHSLTYAPWPSYDESKIKTDTYDMMIQVNGKLRGSITADVNESDDEIKQAALANDNVQKFTAGKDIKKIIVVPRKIVNIV
AK

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI7661872, Length=878, Percent_Identity=32.9157175398633, Blast_Score=459, Evalue=1e-129,
Organism=Homo sapiens, GI108773810, Length=297, Percent_Identity=26.2626262626263, Blast_Score=73, Evalue=1e-12,
Organism=Escherichia coli, GI1786861, Length=861, Percent_Identity=40.8826945412311, Blast_Score=647, Evalue=0.0,
Organism=Escherichia coli, GI1790708, Length=390, Percent_Identity=23.8461538461538, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71997510, Length=790, Percent_Identity=30.5063291139241, Blast_Score=326, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI71997517, Length=790, Percent_Identity=30.5063291139241, Blast_Score=325, Evalue=4e-89,
Organism=Caenorhabditis elegans, GI212645227, Length=315, Percent_Identity=33.015873015873, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17554638, Length=274, Percent_Identity=25.5474452554745, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17541896, Length=393, Percent_Identity=23.6641221374046, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323414, Length=850, Percent_Identity=34.4705882352941, Blast_Score=460, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6321531, Length=905, Percent_Identity=22.3204419889503, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6325097, Length=225, Percent_Identity=28.4444444444444, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI21355409, Length=775, Percent_Identity=34.1935483870968, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI281366294, Length=797, Percent_Identity=23.7139272271016, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28574733, Length=267, Percent_Identity=27.3408239700375, Blast_Score=78, Evalue=3e-14,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYL_LACC3 (Q03AT8)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_806126.1
- ProteinModelPortal:   Q03AT8
- SMR:   Q03AT8
- STRING:   Q03AT8
- GeneID:   4421698
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_0883
- eggNOG:   COG0495
- HOGENOM:   HBG693845
- OMA:   ERGNFPV
- ProtClustDB:   PRK00390
- BioCyc:   LCAS321967:LSEI_0883-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00049_B
- InterPro:   IPR001412
- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11946:SF7
- PRINTS:   PR00985
- TIGRFAMs:   TIGR00396

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1; SSF47323 tRNAsyn_1a_bind; SSF50677 ValRS_IleRS_edit

EC number: =6.1.1.4

Molecular weight: Translated: 91528; Mature: 91397

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 578-578

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYDHHEIDKKWQRYWAEHNEFNTTTDPKKPNYYALDMFPYPSGQGLHVGHPEGYTATDI
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEECCCCCCCCHHHH
VARMKRMQGFNVLHPMGWDAFGLPAEQYALNTGHNPKTFTKQNIETFKRQINSLGFSYDW
HHHHHHHCCCCCCCCCCCCCCCCCHHHHEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEC
NREINTTDPNYYKWTQWIFEQLYKHGLAYEAEVPVNWSPDLGTVVANEEVIDGKTERGGF
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEECCHHCCCCCCCCCC
PVVRKPMRQWMLKITAYAEKLLTDLDDLDWPESIKQMQRNWIGKSTGAQITFRVTDSHEP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
FDVFTTRPDTLFGATYVVMAPEHELVQKITTPAQQAVVDAYIDEAAHKSDLDRTALDKEK
CEEEECCCCCCCCCEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHCHHH
TGVWTGAYATNPVNGEKLPIWISDYVLASYGTGAIMSVPAHDDRDYAFAKKFGIEIKPVI
CCEEECCCCCCCCCCCEEEEEEECHHHHHCCCCCEEECCCCCCCCHHHHHHHCCEEEEEE
EGGNVDEAAYTGDGVHINSGFLDGLNEHDAIDRMIKWLEDKGIGSAKINYKLRDWVFSRQ
ECCCCCCCEECCCCEEECCCHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHH
RYWGEPIPVIHWEDGETTLVPEDELPLTLPEEADIKPSGTGESPLANLTDWVNVVDKNGR
HHCCCCCCEEEECCCCEEECCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
KGKRETNTMPQWAGSSWYFLRFVDPHNKEALADYDKLKAWMPVDLYIGGAEHAVLHLLYA
CCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHH
RFWNLFLYDIGAIPNKEPFQRLFNQGMILGDNHEKMSKSKGNVVNPDDVVDEYGADTLRL
HHHHHHHHHHCCCCCCHHHHHHHHCCCEECCCHHHHHHCCCCCCCHHHHHHHHCCHHHHH
YEMFMGPLDAGIAWSTKGLAGARKFLDRVWSAFIDDEGKLRDRITTINDGRLDKVYNETV
HHHHHCHHCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHEEECCCCCHHHHHHHHH
KKVTEDYDALHFNTAISQMMVFINSARKDDDLPLEYVEGFVKMLAPIAPHLMEEIWSRLG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
HDHSLTYAPWPSYDESKIKTDTYDMMIQVNGKLRGSITADVNESDDEIKQAALANDNVQK
CCCCEEECCCCCCCCCCCCCCCEEEEEEECCEEEEEEEECCCCCHHHHHHHHHCCCCHHH
FTAGKDIKKIIVVPRKIVNIVAK
HHCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AYDHHEIDKKWQRYWAEHNEFNTTTDPKKPNYYALDMFPYPSGQGLHVGHPEGYTATDI
CCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCEECCCCCCCCHHHH
VARMKRMQGFNVLHPMGWDAFGLPAEQYALNTGHNPKTFTKQNIETFKRQINSLGFSYDW
HHHHHHHCCCCCCCCCCCCCCCCCHHHHEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEC
NREINTTDPNYYKWTQWIFEQLYKHGLAYEAEVPVNWSPDLGTVVANEEVIDGKTERGGF
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEECCHHCCCCCCCCCC
PVVRKPMRQWMLKITAYAEKLLTDLDDLDWPESIKQMQRNWIGKSTGAQITFRVTDSHEP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCC
FDVFTTRPDTLFGATYVVMAPEHELVQKITTPAQQAVVDAYIDEAAHKSDLDRTALDKEK
CEEEECCCCCCCCCEEEEECCCHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHCHHH
TGVWTGAYATNPVNGEKLPIWISDYVLASYGTGAIMSVPAHDDRDYAFAKKFGIEIKPVI
CCEEECCCCCCCCCCCEEEEEEECHHHHHCCCCCEEECCCCCCCCHHHHHHHCCEEEEEE
EGGNVDEAAYTGDGVHINSGFLDGLNEHDAIDRMIKWLEDKGIGSAKINYKLRDWVFSRQ
ECCCCCCCEECCCCEEECCCHHCCCCHHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHH
RYWGEPIPVIHWEDGETTLVPEDELPLTLPEEADIKPSGTGESPLANLTDWVNVVDKNGR
HHCCCCCCEEEECCCCEEECCCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCC
KGKRETNTMPQWAGSSWYFLRFVDPHNKEALADYDKLKAWMPVDLYIGGAEHAVLHLLYA
CCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHH
RFWNLFLYDIGAIPNKEPFQRLFNQGMILGDNHEKMSKSKGNVVNPDDVVDEYGADTLRL
HHHHHHHHHHCCCCCCHHHHHHHHCCCEECCCHHHHHHCCCCCCCHHHHHHHHCCHHHHH
YEMFMGPLDAGIAWSTKGLAGARKFLDRVWSAFIDDEGKLRDRITTINDGRLDKVYNETV
HHHHHCHHCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHEEECCCCCHHHHHHHHH
KKVTEDYDALHFNTAISQMMVFINSARKDDDLPLEYVEGFVKMLAPIAPHLMEEIWSRLG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
HDHSLTYAPWPSYDESKIKTDTYDMMIQVNGKLRGSITADVNESDDEIKQAALANDNVQK
CCCCEEECCCCCCCCCCCCCCCEEEEEEECCEEEEEEEECCCCCHHHHHHHHHCCCCHHH
FTAGKDIKKIIVVPRKIVNIVAK
HHCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA