Click here to switch to the map view.

The map label for this gene is xylS [H]

Identifier: 116493968

GI number: 116493968

Start: 405430

End: 407673

Strand: Direct

Name: xylS [H]

Synonym: LSEI_0404

Alternate gene names: 116493968

Gene position: 405430-407673 (Clockwise)

Preceding gene: 116493967

Following gene: 116493969

Centisome position: 14.0

GC content: 46.43

Gene sequence:

>2244_bases
TTGAATCAGAAGAATCAAGTCAGCGGGGATCATTATCGGTTTACCGTCATTACAAATAAGATGATCCGCATGGAATATCA
ATCTGCCGGTCAGTTTGAAGATGCAACGACGCAAACAGTGGTTAATCGCGATTTTGGCAAGCCGGATTTTCAGGTGATCC
GTGATCAAGAAGGTTTTGCTGTCCAAATCGAGACAGATTCATTTCACCTCTATTATCGCGGTGGTGAATTCAACGGAGCC
AACTTGTTCATTGATACAAAATACAATTTTCAGACGCACTATCCGCGTTGGCATTACGGTGATCCCGATGTCAAGAACTT
GCTTGGAACGGCCCGAACGTTAGATGGCGCAGATGGTGCTGTATCGCTGGATTCAGGGATTATGTCCAAAGACGGTTTTA
CTATCTTGGATGACTCAACCAGTATGCTGCAAGCTGGCACATCGATCAGGAATCGGCCTGTTGAGGAAATTGATCTTTAT
GGCTTTGCATACGGTCACGACTATCGCAAGGCTTTGTATGATTATTATCAGCTCACTGGATTTCCGCCGATGGTACCACG
TTTTGCGTTAGGTAATTGGTGGAGTCGCTTCTATCCTTATACGCAAGAAAGTTACCTTGCGCTCATGGATCGTTTCCAAA
AAGCCGGTATTCCAATAGCGGTCGCTGTCTTGGATATGAACTGGCATGTCACAGATATTCCGACGAAGTATGGCAGCGGT
TGGACTGGATATACTTGGAATAAAAAACTGTTCCCGGAACCAGAAAAATTATTGGCAACCTTACACGCACAAGGCAAACA
TGTCACGCTCAATGTGCACCCAGCTGCTGGTATCCGTCCTTCTGAAGTGCAATATCCAGAAGTTGCTAAGGCTGTCGGCA
TTGATCCAGCCTCTAAGCAGCCAGTGTTGTTTGATTTAAACAATCGAAAATTTATTCAAGCCTATTTTAACTTGGTTCAT
CATCCGCTTGAAAAACAGGGGATTGATTTCTGGTGGCTTGATTGGCAGCAGGGCGCTGCACGATCACGTGCGCAGATTGA
TCCATTATGGTCGCTTAACGTGCTGCATTTTCTGGATCAGGTGAAAGAAAAGAAGGATCAAGCGCTGATCTTGTCACGCT
ATGCTGGACCTGGCAGTCACCGGTATCCAATTGGGTTTTCTGGCGATTCCATTGCCAGTTGGCGATCATTAACTTTCCAA
CCGTACTTCACAGCAACGGCCACGAATATTGGCTACACCTGGTGGAGTCATGATATCGGCGGGCATATGCACGGCAGCTA
TGATCCTGAATTGTCACTACGCTGGCTGCAGTTCGGTGTTTTCAGTCCGATTATGCGGTTGCACAGTTCGGACAATCCGT
TTATGGGCAAGGAACCATGGCAATATGATCTGGAAACGGATAAGAGCATGACACGTTTCATGCGATTGCGCGCTCAGCTT
GTCCCTTATCTGGCGACGGCGGATGTGTTGACACATCAGCAAGGCATGCCATTGATTGAACCTGTTTATTATCGATATCC
AGAAGTAAAAGAAGCTTATCAGTTCAAAAATGAGTACTTCTTCGGGTCAGAAATGCTGGTCGTACCGATCACAGCGCCTA
GTGATGACACAACGGGCTTAGCCAGTGCAGAAGGTTATGTGCCAGCCGGTACCTGGACCGATTTATTTACCCATCAGCAA
TATACTGGTCCGGCAGTTGTTAAGTTCTATCGCAACAAGTTTCAGTATCCGGTTCTTGTGCGCTCAGGCGGCATCGTGCC
ATTAGCTGATGACGCTATGGCGGCCATTGATGATCTGCCCGAAGCTATGACCGTTACGCTTTTCCCTGGCAAGCAACATG
CCTATGTATTACATGAACAGACAGCGGCTGGAGAAGCGCAAACCAAATTTTCTTGGGATCCCGTGGCCGGTACATTTGGG
ATGACCGTTACTGATCCTAATCACATCATTCCCGAGAAGCGGACCTATCAATTACAAATTGTGGGCGTTAAAACCACAAT
GAAACCATTCTCAGGACGATTTGATCAGCGACTGACACTTGATTTAGAAGCCGAAGATCAACAGGCAATTAAACTGCAGC
ATATCTTTGCAATTCTGCAACATGCTAAAGTTGCGTTTGATCTGAAAAAGCAGTTGTGGCAAAGCGTTAACGATATGCCA
GCAAGTAGAGCAGCATTAACTGTTGCCAGTCTCGCACCGGCAACACTGAGCGATGCACTTTTGGAGATACTTTTGAATGA
TTAG

Upstream 100 bases:

>100_bases
TTGTCCTGATTTTAGCCATTGCACTCGTAGGTTCCTTCTTTGGTATTCTCGGGATTGAACCAACAAAGTAATCATGATGA
AAGATTGAGGGGTTGATATT

Downstream 100 bases:

>100_bases
GCCTGAAATGACCGTCCTTTGGTCATTGCGGATAAGGTCCTTAGGTGCAGGTTTCTGGTCTGGTAAACGTGGTTAGAGGA
GGAAGATTCATGAAACGCAC

Product: alpha-glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 747; Mature: 747

Protein sequence:

>747_residues
MNQKNQVSGDHYRFTVITNKMIRMEYQSAGQFEDATTQTVVNRDFGKPDFQVIRDQEGFAVQIETDSFHLYYRGGEFNGA
NLFIDTKYNFQTHYPRWHYGDPDVKNLLGTARTLDGADGAVSLDSGIMSKDGFTILDDSTSMLQAGTSIRNRPVEEIDLY
GFAYGHDYRKALYDYYQLTGFPPMVPRFALGNWWSRFYPYTQESYLALMDRFQKAGIPIAVAVLDMNWHVTDIPTKYGSG
WTGYTWNKKLFPEPEKLLATLHAQGKHVTLNVHPAAGIRPSEVQYPEVAKAVGIDPASKQPVLFDLNNRKFIQAYFNLVH
HPLEKQGIDFWWLDWQQGAARSRAQIDPLWSLNVLHFLDQVKEKKDQALILSRYAGPGSHRYPIGFSGDSIASWRSLTFQ
PYFTATATNIGYTWWSHDIGGHMHGSYDPELSLRWLQFGVFSPIMRLHSSDNPFMGKEPWQYDLETDKSMTRFMRLRAQL
VPYLATADVLTHQQGMPLIEPVYYRYPEVKEAYQFKNEYFFGSEMLVVPITAPSDDTTGLASAEGYVPAGTWTDLFTHQQ
YTGPAVVKFYRNKFQYPVLVRSGGIVPLADDAMAAIDDLPEAMTVTLFPGKQHAYVLHEQTAAGEAQTKFSWDPVAGTFG
MTVTDPNHIIPEKRTYQLQIVGVKTTMKPFSGRFDQRLTLDLEAEDQQAIKLQHIFAILQHAKVAFDLKKQLWQSVNDMP
ASRAALTVASLAPATLSDALLEILLND

Sequences:

>Translated_747_residues
MNQKNQVSGDHYRFTVITNKMIRMEYQSAGQFEDATTQTVVNRDFGKPDFQVIRDQEGFAVQIETDSFHLYYRGGEFNGA
NLFIDTKYNFQTHYPRWHYGDPDVKNLLGTARTLDGADGAVSLDSGIMSKDGFTILDDSTSMLQAGTSIRNRPVEEIDLY
GFAYGHDYRKALYDYYQLTGFPPMVPRFALGNWWSRFYPYTQESYLALMDRFQKAGIPIAVAVLDMNWHVTDIPTKYGSG
WTGYTWNKKLFPEPEKLLATLHAQGKHVTLNVHPAAGIRPSEVQYPEVAKAVGIDPASKQPVLFDLNNRKFIQAYFNLVH
HPLEKQGIDFWWLDWQQGAARSRAQIDPLWSLNVLHFLDQVKEKKDQALILSRYAGPGSHRYPIGFSGDSIASWRSLTFQ
PYFTATATNIGYTWWSHDIGGHMHGSYDPELSLRWLQFGVFSPIMRLHSSDNPFMGKEPWQYDLETDKSMTRFMRLRAQL
VPYLATADVLTHQQGMPLIEPVYYRYPEVKEAYQFKNEYFFGSEMLVVPITAPSDDTTGLASAEGYVPAGTWTDLFTHQQ
YTGPAVVKFYRNKFQYPVLVRSGGIVPLADDAMAAIDDLPEAMTVTLFPGKQHAYVLHEQTAAGEAQTKFSWDPVAGTFG
MTVTDPNHIIPEKRTYQLQIVGVKTTMKPFSGRFDQRLTLDLEAEDQQAIKLQHIFAILQHAKVAFDLKKQLWQSVNDMP
ASRAALTVASLAPATLSDALLEILLND
>Mature_747_residues
MNQKNQVSGDHYRFTVITNKMIRMEYQSAGQFEDATTQTVVNRDFGKPDFQVIRDQEGFAVQIETDSFHLYYRGGEFNGA
NLFIDTKYNFQTHYPRWHYGDPDVKNLLGTARTLDGADGAVSLDSGIMSKDGFTILDDSTSMLQAGTSIRNRPVEEIDLY
GFAYGHDYRKALYDYYQLTGFPPMVPRFALGNWWSRFYPYTQESYLALMDRFQKAGIPIAVAVLDMNWHVTDIPTKYGSG
WTGYTWNKKLFPEPEKLLATLHAQGKHVTLNVHPAAGIRPSEVQYPEVAKAVGIDPASKQPVLFDLNNRKFIQAYFNLVH
HPLEKQGIDFWWLDWQQGAARSRAQIDPLWSLNVLHFLDQVKEKKDQALILSRYAGPGSHRYPIGFSGDSIASWRSLTFQ
PYFTATATNIGYTWWSHDIGGHMHGSYDPELSLRWLQFGVFSPIMRLHSSDNPFMGKEPWQYDLETDKSMTRFMRLRAQL
VPYLATADVLTHQQGMPLIEPVYYRYPEVKEAYQFKNEYFFGSEMLVVPITAPSDDTTGLASAEGYVPAGTWTDLFTHQQ
YTGPAVVKFYRNKFQYPVLVRSGGIVPLADDAMAAIDDLPEAMTVTLFPGKQHAYVLHEQTAAGEAQTKFSWDPVAGTFG
MTVTDPNHIIPEKRTYQLQIVGVKTTMKPFSGRFDQRLTLDLEAEDQQAIKLQHIFAILQHAKVAFDLKKQLWQSVNDMP
ASRAALTVASLAPATLSDALLEILLND

Specific function: Catalyzes the liberation of alpha-xylose from the non- reducing terminal glucose of xyloglucan oligosaccharides. Has high hydrolytic activity on the disaccharide isoprimeverose. Follows a retaining mechanism of substrate hydrolysis [H]

COG id: COG1501

COG function: function code G; Alpha-glucosidases, family 31 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 31 family [H]

Homologues:

Organism=Homo sapiens, GI66346737, Length=451, Percent_Identity=24.6119733924612, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI38202257, Length=479, Percent_Identity=22.1294363256785, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI88900491, Length=257, Percent_Identity=24.9027237354086, Blast_Score=97, Evalue=8e-20,
Organism=Homo sapiens, GI119393895, Length=458, Percent_Identity=22.4890829694323, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI119393893, Length=458, Percent_Identity=22.4890829694323, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI119393891, Length=458, Percent_Identity=22.4890829694323, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI157364974, Length=274, Percent_Identity=22.992700729927, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI2367256, Length=480, Percent_Identity=23.3333333333333, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI2367323, Length=252, Percent_Identity=25.7936507936508, Blast_Score=79, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI71985706, Length=235, Percent_Identity=26.8085106382979, Blast_Score=76, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI32563849, Length=231, Percent_Identity=26.8398268398268, Blast_Score=76, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI71991189, Length=223, Percent_Identity=25.1121076233184, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17560798, Length=223, Percent_Identity=23.7668161434978, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17560800, Length=223, Percent_Identity=23.7668161434978, Blast_Score=74, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319706, Length=230, Percent_Identity=25.6521739130435, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24643749, Length=235, Percent_Identity=28.5106382978723, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24643753, Length=235, Percent_Identity=28.5106382978723, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24643751, Length=235, Percent_Identity=28.5106382978723, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24643746, Length=235, Percent_Identity=28.5106382978723, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21357605, Length=235, Percent_Identity=28.5106382978723, Blast_Score=92, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011013
- InterPro:   IPR000322
- InterPro:   IPR017853 [H]

Pfam domain/function: PF01055 Glyco_hydro_31 [H]

EC number: NA

Molecular weight: Translated: 84858; Mature: 84858

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQKNQVSGDHYRFTVITNKMIRMEYQSAGQFEDATTQTVVNRDFGKPDFQVIRDQEGFA
CCCCCCCCCCEEEEEEEECEEEEEEHHCCCCCCCCHHHHHHHCCCCCCCCEEEECCCCCE
VQIETDSFHLYYRGGEFNGANLFIDTKYNFQTHYPRWHYGDPDVKNLLGTARTLDGADGA
EEEECCCEEEEEECCCCCCCEEEEEEEECCEECCCCCCCCCCHHHHHHHHHHCCCCCCCC
VSLDSGIMSKDGFTILDDSTSMLQAGTSIRNRPVEEIDLYGFAYGHDYRKALYDYYQLTG
EEECCCCCCCCCCEEEECCHHHHHCCCHHHCCCCHHHEEEEEEECCHHHHHHHHHHHHCC
FPPMVPRFALGNWWSRFYPYTQESYLALMDRFQKAGIPIAVAVLDMNWHVTDIPTKYGSG
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEECCEEEEECCCCCCCC
WTGYTWNKKLFPEPEKLLATLHAQGKHVTLNVHPAAGIRPSEVQYPEVAKAVGIDPASKQ
CCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHCCCCCCCC
PVLFDLNNRKFIQAYFNLVHHPLEKQGIDFWWLDWQQGAARSRAQIDPLWSLNVLHFLDQ
CEEEECCCCHHHHHHHHHHHCCHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
VKEKKDQALILSRYAGPGSHRYPIGFSGDSIASWRSLTFQPYFTATATNIGYTWWSHDIG
HHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHCEEECEEEEEEECCCCEEEECCCCC
GHMHGSYDPELSLRWLQFGVFSPIMRLHSSDNPFMGKEPWQYDLETDKSMTRFMRLRAQL
CCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHH
VPYLATADVLTHQQGMPLIEPVYYRYPEVKEAYQFKNEYFFGSEMLVVPITAPSDDTTGL
HHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHCCEEECCCEEEEEEECCCCCCCCC
ASAEGYVPAGTWTDLFTHQQYTGPAVVKFYRNKFQYPVLVRSGGIVPLADDAMAAIDDLP
CCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHCCCCEEEEECCCEEEECCHHHHHHHCCC
EAMTVTLFPGKQHAYVLHEQTAAGEAQTKFSWDPVAGTFGMTVTDPNHIIPEKRTYQLQI
CCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEE
VGVKTTMKPFSGRFDQRLTLDLEAEDQQAIKLQHIFAILQHAKVAFDLKKQLWQSVNDMP
EEEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ASRAALTVASLAPATLSDALLEILLND
CHHHHEEHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MNQKNQVSGDHYRFTVITNKMIRMEYQSAGQFEDATTQTVVNRDFGKPDFQVIRDQEGFA
CCCCCCCCCCEEEEEEEECEEEEEEHHCCCCCCCCHHHHHHHCCCCCCCCEEEECCCCCE
VQIETDSFHLYYRGGEFNGANLFIDTKYNFQTHYPRWHYGDPDVKNLLGTARTLDGADGA
EEEECCCEEEEEECCCCCCCEEEEEEEECCEECCCCCCCCCCHHHHHHHHHHCCCCCCCC
VSLDSGIMSKDGFTILDDSTSMLQAGTSIRNRPVEEIDLYGFAYGHDYRKALYDYYQLTG
EEECCCCCCCCCCEEEECCHHHHHCCCHHHCCCCHHHEEEEEEECCHHHHHHHHHHHHCC
FPPMVPRFALGNWWSRFYPYTQESYLALMDRFQKAGIPIAVAVLDMNWHVTDIPTKYGSG
CCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEECCEEEEECCCCCCCC
WTGYTWNKKLFPEPEKLLATLHAQGKHVTLNVHPAAGIRPSEVQYPEVAKAVGIDPASKQ
CCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHHHHCCCCCCCC
PVLFDLNNRKFIQAYFNLVHHPLEKQGIDFWWLDWQQGAARSRAQIDPLWSLNVLHFLDQ
CEEEECCCCHHHHHHHHHHHCCHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHH
VKEKKDQALILSRYAGPGSHRYPIGFSGDSIASWRSLTFQPYFTATATNIGYTWWSHDIG
HHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHCEEECEEEEEEECCCCEEEECCCCC
GHMHGSYDPELSLRWLQFGVFSPIMRLHSSDNPFMGKEPWQYDLETDKSMTRFMRLRAQL
CCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHH
VPYLATADVLTHQQGMPLIEPVYYRYPEVKEAYQFKNEYFFGSEMLVVPITAPSDDTTGL
HHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHHHHCCEEECCCEEEEEEECCCCCCCCC
ASAEGYVPAGTWTDLFTHQQYTGPAVVKFYRNKFQYPVLVRSGGIVPLADDAMAAIDDLP
CCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHCCCCEEEEECCCEEEECCHHHHHHHCCC
EAMTVTLFPGKQHAYVLHEQTAAGEAQTKFSWDPVAGTFGMTVTDPNHIIPEKRTYQLQI
CCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEE
VGVKTTMKPFSGRFDQRLTLDLEAEDQQAIKLQHIFAILQHAKVAFDLKKQLWQSVNDMP
EEEEEECCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ASRAALTVASLAPATLSDALLEILLND
CHHHHEEHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10801892; 11427726 [H]