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The map label for this gene is rbsB [H]

Identifier: 116493878

GI number: 116493878

Start: 310539

End: 311498

Strand: Direct

Name: rbsB [H]

Synonym: LSEI_0310

Alternate gene names: 116493878

Gene position: 310539-311498 (Clockwise)

Preceding gene: 116493877

Following gene: 116493880

Centisome position: 10.73

GC content: 46.98

Gene sequence:

>960_bases
ATGAAACAATCACTCAAACGCTTTTTAATGGTCGCTGTTGTTGCGACGGCAGCTTTATTCACCTTAACCGCATGCGGCGG
CACAGGACTCAGCTCCAAATCGGACAGCAACACAAAAGTGACCAAGAAAGCGCCCAAAGATCTGAAAGTTGGCGTCTCAC
TTTCAACGCTGTCCAATCCATTCTTCGTTTCTGTTCGTAATGGGATCCAAGATTTAGCCAAAAAGAACAAAACCAATGTT
CAAGTTTCCGATGCTCAAAATGATACCGCTAAGCAAAACAGTAACATTGAAGATTTAATTCAAAAGAAGGTTGATGTGCT
GATCATCAACCCAGTTGACTCAAGTGCCATTACACCAGCCGTTAAAGATGCCAATGATGCCGGCATCCCGGTGATCACCG
TTGATCGGTCGAGTGACGGCGGTAAAGTTTTGACCTTAGTCGCTTCTAACTCCACAAAAGGTGGCCAAATGGCGGCTAAG
TATATGATCGAAAAACTTGGTAAAGACGCTAAAATTGCTGAATTGCAAGGCATTCCAGGTGCATCCGCAACCCGTGAACG
TGGTAAAGGGTTCGATGGCGCTGCTAAAGGCAAACTCGATATCGTGTCCAAGCAAACAGCCGGCTTCGACCGCGCTAAAG
GGCTGACCGTTACCGAAAATATTTTGCAGGGTAATGGTGACATTGTCGGTATCTTCTCGCAAAATGATGAAATGGCATTA
GGGGCGGTTCAAGCAGTCAAAGCCGCTGGTAAAAAGATCACCATTGTCGGGTTCGATGGCGAAGCGGACGGGATTAAAGC
CGTTAAGGCCGGCGACATGGCTGCAACGGTTGCCCAGAAGCCAGAAGAAATGGGCCGATTGGCTTTACAAGCAGCCTATG
ATCATTTCAACGGTAAAACGGTGAAGAAGAACGTTGAATCACCATTGTCACTTGTGACAACCGAGGATGCGAACAAGTAA

Upstream 100 bases:

>100_bases
TGATTGCGGTTCTGCTCGATCGCCGGTCTTCTAATAATGGGTAACAAAACGTGAGCGCGGGCTCGCGGCAAAAGACAAAA
CTCTAGGGGGAGAAATTGAC

Downstream 100 bases:

>100_bases
GCTTCATGCCTAGATTGCAATAATAAAGTTACCACCTAGAAAGAGGCTTGCTCACTGCTTGAACTGGGGTTTGCCAACGG
AGACATTTTCTAGGTTTGTT

Product: ABC-type sugar transport system, periplasmic component

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKQSLKRFLMVAVVATAALFTLTACGGTGLSSKSDSNTKVTKKAPKDLKVGVSLSTLSNPFFVSVRNGIQDLAKKNKTNV
QVSDAQNDTAKQNSNIEDLIQKKVDVLIINPVDSSAITPAVKDANDAGIPVITVDRSSDGGKVLTLVASNSTKGGQMAAK
YMIEKLGKDAKIAELQGIPGASATRERGKGFDGAAKGKLDIVSKQTAGFDRAKGLTVTENILQGNGDIVGIFSQNDEMAL
GAVQAVKAAGKKITIVGFDGEADGIKAVKAGDMAATVAQKPEEMGRLALQAAYDHFNGKTVKKNVESPLSLVTTEDANK

Sequences:

>Translated_319_residues
MKQSLKRFLMVAVVATAALFTLTACGGTGLSSKSDSNTKVTKKAPKDLKVGVSLSTLSNPFFVSVRNGIQDLAKKNKTNV
QVSDAQNDTAKQNSNIEDLIQKKVDVLIINPVDSSAITPAVKDANDAGIPVITVDRSSDGGKVLTLVASNSTKGGQMAAK
YMIEKLGKDAKIAELQGIPGASATRERGKGFDGAAKGKLDIVSKQTAGFDRAKGLTVTENILQGNGDIVGIFSQNDEMAL
GAVQAVKAAGKKITIVGFDGEADGIKAVKAGDMAATVAQKPEEMGRLALQAAYDHFNGKTVKKNVESPLSLVTTEDANK
>Mature_319_residues
MKQSLKRFLMVAVVATAALFTLTACGGTGLSSKSDSNTKVTKKAPKDLKVGVSLSTLSNPFFVSVRNGIQDLAKKNKTNV
QVSDAQNDTAKQNSNIEDLIQKKVDVLIINPVDSSAITPAVKDANDAGIPVITVDRSSDGGKVLTLVASNSTKGGQMAAK
YMIEKLGKDAKIAELQGIPGASATRERGKGFDGAAKGKLDIVSKQTAGFDRAKGLTVTENILQGNGDIVGIFSQNDEMAL
GAVQAVKAAGKKITIVGFDGEADGIKAVKAGDMAATVAQKPEEMGRLALQAAYDHFNGKTVKKNVESPLSLVTTEDANK

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=268, Percent_Identity=45.5223880597015, Blast_Score=228, Evalue=5e-61,
Organism=Escherichia coli, GI1790526, Length=260, Percent_Identity=35, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1790674, Length=226, Percent_Identity=32.3008849557522, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789990, Length=294, Percent_Identity=26.530612244898, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790194, Length=262, Percent_Identity=23.6641221374046, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788898, Length=234, Percent_Identity=28.2051282051282, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1788473, Length=259, Percent_Identity=28.957528957529, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 33251; Mature: 33251

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQSLKRFLMVAVVATAALFTLTACGGTGLSSKSDSNTKVTKKAPKDLKVGVSLSTLSNP
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCEEEEEEHHHCCCC
FFVSVRNGIQDLAKKNKTNVQVSDAQNDTAKQNSNIEDLIQKKVDVLIINPVDSSAITPA
EEEEHHHHHHHHHHCCCCCEEEECCCCCHHHCCCCHHHHHHCCCCEEEECCCCCCCCCCC
VKDANDAGIPVITVDRSSDGGKVLTLVASNSTKGGQMAAKYMIEKLGKDAKIAELQGIPG
CCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEHCCCCCC
ASATRERGKGFDGAAKGKLDIVSKQTAGFDRAKGLTVTENILQGNGDIVGIFSQNDEMAL
CCCHHHHCCCCCCCCCCEEEEEECCCCCCHHHCCCEEEHHHHCCCCCEEEEEECCCCHHH
GAVQAVKAAGKKITIVGFDGEADGIKAVKAGDMAATVAQKPEEMGRLALQAAYDHFNGKT
HHHHHHHHCCCEEEEEEECCCCCCEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHCCCCE
VKKNVESPLSLVTTEDANK
EHHHHCCCEEEEECCCCCC
>Mature Secondary Structure
MKQSLKRFLMVAVVATAALFTLTACGGTGLSSKSDSNTKVTKKAPKDLKVGVSLSTLSNP
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCCCCEEEEEEHHHCCCC
FFVSVRNGIQDLAKKNKTNVQVSDAQNDTAKQNSNIEDLIQKKVDVLIINPVDSSAITPA
EEEEHHHHHHHHHHCCCCCEEEECCCCCHHHCCCCHHHHHHCCCCEEEECCCCCCCCCCC
VKDANDAGIPVITVDRSSDGGKVLTLVASNSTKGGQMAAKYMIEKLGKDAKIAELQGIPG
CCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEHCCCCCC
ASATRERGKGFDGAAKGKLDIVSKQTAGFDRAKGLTVTENILQGNGDIVGIFSQNDEMAL
CCCHHHHCCCCCCCCCCEEEEEECCCCCCHHHCCCEEEHHHHCCCCCEEEEEECCCCHHH
GAVQAVKAAGKKITIVGFDGEADGIKAVKAGDMAATVAQKPEEMGRLALQAAYDHFNGKT
HHHHHHHHCCCEEEEEEECCCCCCEEEEECCCHHHHHHCCHHHHHHHHHHHHHHHCCCCE
VKKNVESPLSLVTTEDANK
EHHHHCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]