Click here to switch to the map view.

The map label for this gene is thiE

Identifier: 116493868

GI number: 116493868

Start: 302160

End: 302801

Strand: Direct

Name: thiE

Synonym: LSEI_0300

Alternate gene names: 116493868

Gene position: 302160-302801 (Clockwise)

Preceding gene: 116493867

Following gene: 116493869

Centisome position: 10.44

GC content: 51.09

Gene sequence:

>642_bases
ATGAACGCAACAGATCTAAAACTGTACTTAGTCACCCATCGTTACGATGACAACGAAGCAACTTTTTTGGCTAAAATCGC
GGCTGCTTGTGAAAATAGCGTCACGATGGTGCAATTACGCGAAAAGATGCTGAGCACTCGAGCCTATTTTGAATTGGCAC
AACGGGTTAAGCTCATCACTGACCGTTATCAGATCCCGCTGATTATTGATGATCGAGTCGACATTTGTTTAGCTGTAGAT
GCAGCTGGTGTGCATATCGGCGATGATGAGCTGCCTGTTGCGATGACCCGCCAGTTGATCGGTCCTGACAAGGTATTGGG
CGTTTCCACAAAAACCGTCGAGACGGCGGTTGCCGCGGTTGCCGCTGGTGCTGATTACTTGGGGGTTGGCGCCATTTTCC
CGACGCAGACGAAGGCAAACGCAGCGGTGACCCCGATTGCGACATTAAAGGCGATCACTGCACAAGTTGCCGTGCCGGTC
GTGGCTATTGGCGGCGTCAAAGAAGCCAACTTGGCGACGTTTAAAGATACCGGGATCGCCGGGGTTGCGATTGTGAGCGA
AATCATGCAGGCACCTGATATCGCTCACAAAGTGCAGGCTTTGCGCACAAAATTAAAGGCGGTGTTACCAAATGACCGTT
AA

Upstream 100 bases:

>100_bases
GAACCGGCGGACTGGCGGCATTTCGCGAAGGTACCTTGAATCAACTTTCCTTACTGGCTGCCACCGCTGACTGGCTTCAA
ATGGTGAAAGGGGAAGCGTT

Downstream 100 bases:

>100_bases
TCGCACACCGCAAGTCGTCACCATCGCTGGTTCCGATTCCGGTGGCGGGGCAGGGTTGCAAGCGGACTTGAAAACTTTTC
AGGCCCGACACGTCTTTGGG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MNATDLKLYLVTHRYDDNEATFLAKIAAACENSVTMVQLREKMLSTRAYFELAQRVKLITDRYQIPLIIDDRVDICLAVD
AAGVHIGDDELPVAMTRQLIGPDKVLGVSTKTVETAVAAVAAGADYLGVGAIFPTQTKANAAVTPIATLKAITAQVAVPV
VAIGGVKEANLATFKDTGIAGVAIVSEIMQAPDIAHKVQALRTKLKAVLPNDR

Sequences:

>Translated_213_residues
MNATDLKLYLVTHRYDDNEATFLAKIAAACENSVTMVQLREKMLSTRAYFELAQRVKLITDRYQIPLIIDDRVDICLAVD
AAGVHIGDDELPVAMTRQLIGPDKVLGVSTKTVETAVAAVAAGADYLGVGAIFPTQTKANAAVTPIATLKAITAQVAVPV
VAIGGVKEANLATFKDTGIAGVAIVSEIMQAPDIAHKVQALRTKLKAVLPNDR
>Mature_213_residues
MNATDLKLYLVTHRYDDNEATFLAKIAAACENSVTMVQLREKMLSTRAYFELAQRVKLITDRYQIPLIIDDRVDICLAVD
AAGVHIGDDELPVAMTRQLIGPDKVLGVSTKTVETAVAAVAAGADYLGVGAIFPTQTKANAAVTPIATLKAITAQVAVPV
VAIGGVKEANLATFKDTGIAGVAIVSEIMQAPDIAHKVQALRTKLKAVLPNDR

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=134, Percent_Identity=34.3283582089552, Blast_Score=61, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6325042, Length=223, Percent_Identity=37.219730941704, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_LACC3 (Q03CB2)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_805602.1
- ProteinModelPortal:   Q03CB2
- SMR:   Q03CB2
- STRING:   Q03CB2
- GeneID:   4419767
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_0300
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   QVRSKPI
- ProtClustDB:   PRK00043
- BioCyc:   LCAS321967:LSEI_0300-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 22638; Mature: 22638

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATDLKLYLVTHRYDDNEATFLAKIAAACENSVTMVQLREKMLSTRAYFELAQRVKLIT
CCCCEEEEEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRYQIPLIIDDRVDICLAVDAAGVHIGDDELPVAMTRQLIGPDKVLGVSTKTVETAVAAV
CCEEEEEEECCCCEEEEEEECCCEEECCCCCCHHHHHHHCCCCCEECCCHHHHHHHHHHH
AAGADYLGVGAIFPTQTKANAAVTPIATLKAITAQVAVPVVAIGGVKEANLATFKDTGIA
HCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCEEECCCCCH
GVAIVSEIMQAPDIAHKVQALRTKLKAVLPNDR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNATDLKLYLVTHRYDDNEATFLAKIAAACENSVTMVQLREKMLSTRAYFELAQRVKLIT
CCCCEEEEEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRYQIPLIIDDRVDICLAVDAAGVHIGDDELPVAMTRQLIGPDKVLGVSTKTVETAVAAV
CCEEEEEEECCCCEEEEEEECCCEEECCCCCCHHHHHHHCCCCCEECCCHHHHHHHHHHH
AAGADYLGVGAIFPTQTKANAAVTPIATLKAITAQVAVPVVAIGGVKEANLATFKDTGIA
HCCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCEEECCCCCH
GVAIVSEIMQAPDIAHKVQALRTKLKAVLPNDR
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA