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The map label for this gene is thiM

Identifier: 116493867

GI number: 116493867

Start: 301321

End: 302163

Strand: Direct

Name: thiM

Synonym: LSEI_0299

Alternate gene names: 116493867

Gene position: 301321-302163 (Clockwise)

Preceding gene: 116493866

Following gene: 116493868

Centisome position: 10.41

GC content: 51.6

Gene sequence:

>843_bases
ATGTCAAAAGCAATCACTGACGTTTTTTACACCGCGTTCAAAACCGCCCTGCCATTAACATCATCCCCACTGGTGCAATG
CATTACCAACGAAATCACCGTCGAATCCATGGCTAATGCATTGCTTTACATTGATGCGAAGCCGGTCATGGCTGATGATC
AGCGCGAGTTCCCGGAATTCTTTGCTCAAAGTGATGCGTTGCTGTTGAATCTTGGACACATTTCCGAAGTGCGCCAGCAG
AATCTATTAGCCGCTGGCAAGTTTGCGCAGGCCACCAACCAGCCAACGGTGATTGATTTGGTCGGCGTTTCTGCCACCCA
GTTGCGCTATGATTTAGGCCATCAATTGTTAGTCAATCATCCGAACGTGGTCAAAGGTAACATTTCTGAAATGCGCCGAT
TCGCTGATCTAAAAAGCACAGGCCGCGGCGTTGATGGAAGCCAGTTAGATCAAAGTGTGACCGCCTTGGGAGAACTAGCC
GCGAGCTTGCAGCAGCTGACCCAAGCGTTTCCCACCACTACCTTCTTGGCAACCGGCAAGATTGATCTCGTTGTGAGTGC
TAAGGGAACTTGGTATTTGAAAAATGGGGTGCCGCAGCTGGATCGTTTCACCGGGACTGGTGACATTGTCGGTGCCTTGA
TTGCCGCGCTGTTGGGGACAGGTTTGGACAACGACGCAGCAGTCGTCGTGGCTGTGAGTTACTTCAACTGCTGTGGCGAA
GTAGCAGCTGCGCAGAATCGAACCGGCGGACTGGCGGCATTTCGCGAAGGTACCTTGAATCAACTTTCCTTACTGGCTGC
CACCGCTGACTGGCTTCAAATGGTGAAAGGGGAAGCGTTATGA

Upstream 100 bases:

>100_bases
ATCGATCTCAGGGTCGTTAATCGGCTTGTTTGTCCTATATGAACTTGATCACATTGGCATCACCAAGCGTATTCAAAAAA
TTTTCTAGGAGGCTTTTTCC

Downstream 100 bases:

>100_bases
ACGCAACAGATCTAAAACTGTACTTAGTCACCCATCGTTACGATGACAACGAAGCAACTTTTTTGGCTAAAATCGCGGCT
GCTTGTGAAAATAGCGTCAC

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 280; Mature: 279

Protein sequence:

>280_residues
MSKAITDVFYTAFKTALPLTSSPLVQCITNEITVESMANALLYIDAKPVMADDQREFPEFFAQSDALLLNLGHISEVRQQ
NLLAAGKFAQATNQPTVIDLVGVSATQLRYDLGHQLLVNHPNVVKGNISEMRRFADLKSTGRGVDGSQLDQSVTALGELA
ASLQQLTQAFPTTTFLATGKIDLVVSAKGTWYLKNGVPQLDRFTGTGDIVGALIAALLGTGLDNDAAVVVAVSYFNCCGE
VAAAQNRTGGLAAFREGTLNQLSLLAATADWLQMVKGEAL

Sequences:

>Translated_280_residues
MSKAITDVFYTAFKTALPLTSSPLVQCITNEITVESMANALLYIDAKPVMADDQREFPEFFAQSDALLLNLGHISEVRQQ
NLLAAGKFAQATNQPTVIDLVGVSATQLRYDLGHQLLVNHPNVVKGNISEMRRFADLKSTGRGVDGSQLDQSVTALGELA
ASLQQLTQAFPTTTFLATGKIDLVVSAKGTWYLKNGVPQLDRFTGTGDIVGALIAALLGTGLDNDAAVVVAVSYFNCCGE
VAAAQNRTGGLAAFREGTLNQLSLLAATADWLQMVKGEAL
>Mature_279_residues
SKAITDVFYTAFKTALPLTSSPLVQCITNEITVESMANALLYIDAKPVMADDQREFPEFFAQSDALLLNLGHISEVRQQN
LLAAGKFAQATNQPTVIDLVGVSATQLRYDLGHQLLVNHPNVVKGNISEMRRFADLKSTGRGVDGSQLDQSVTALGELAA
SLQQLTQAFPTTTFLATGKIDLVVSAKGTWYLKNGVPQLDRFTGTGDIVGALIAALLGTGLDNDAAVVVAVSYFNCCGEV
AAAQNRTGGLAAFREGTLNQLSLLAATADWLQMVKGEAL

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=229, Percent_Identity=30.5676855895196, Blast_Score=102, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6325042, Length=255, Percent_Identity=29.0196078431373, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_LACC3 (Q03CB3)

Other databases:

- EMBL:   CP000423
- RefSeq:   YP_805601.1
- ProteinModelPortal:   Q03CB3
- SMR:   Q03CB3
- STRING:   Q03CB3
- GeneID:   4419766
- GenomeReviews:   CP000423_GR
- KEGG:   lca:LSEI_0299
- eggNOG:   COG2145
- HOGENOM:   HBG515811
- OMA:   MGICGEL
- PhylomeDB:   Q03CB3
- ProtClustDB:   CLSK559900
- BioCyc:   LCAS321967:LSEI_0299-MONOMER
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 29780; Mature: 29649

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: BINDING 50-50 BINDING 125-125 BINDING 178-178 BINDING 205-205

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAITDVFYTAFKTALPLTSSPLVQCITNEITVESMANALLYIDAKPVMADDQREFPEF
CCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHH
FAQSDALLLNLGHISEVRQQNLLAAGKFAQATNQPTVIDLVGVSATQLRYDLGHQLLVNH
HHCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHCCCEEEECC
PNVVKGNISEMRRFADLKSTGRGVDGSQLDQSVTALGELAASLQQLTQAFPTTTFLATGK
CCCEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
IDLVVSAKGTWYLKNGVPQLDRFTGTGDIVGALIAALLGTGLDNDAAVVVAVSYFNCCGE
EEEEEECCCCEEECCCCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHH
VAAAQNRTGGLAAFREGTLNQLSLLAATADWLQMVKGEAL
HHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKAITDVFYTAFKTALPLTSSPLVQCITNEITVESMANALLYIDAKPVMADDQREFPEF
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCHHHHHHH
FAQSDALLLNLGHISEVRQQNLLAAGKFAQATNQPTVIDLVGVSATQLRYDLGHQLLVNH
HHCCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHCCCEEEECC
PNVVKGNISEMRRFADLKSTGRGVDGSQLDQSVTALGELAASLQQLTQAFPTTTFLATGK
CCCEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
IDLVVSAKGTWYLKNGVPQLDRFTGTGDIVGALIAALLGTGLDNDAAVVVAVSYFNCCGE
EEEEEECCCCEEECCCCCHHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEHHHHHHHHH
VAAAQNRTGGLAAFREGTLNQLSLLAATADWLQMVKGEAL
HHHHCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA