Click here to switch to the map view.

The map label for this gene is engD [H]

Identifier: 116493786

GI number: 116493786

Start: 209252

End: 210358

Strand: Direct

Name: engD [H]

Synonym: LSEI_0215

Alternate gene names: 116493786

Gene position: 209252-210358 (Clockwise)

Preceding gene: 116493785

Following gene: 116493787

Centisome position: 7.23

GC content: 46.7

Gene sequence:

>1107_bases
ATGGCTTTAACAGCAGGTATCGTAGGACTTCCAAACGTCGGTAAGTCCACGCTTTTTAACGCAATCACTAAAGCAGGCGC
CGAGATGGCGAACTATCCATTCGCTACAATCGACCCGAATGTCGGGATGGTCGAAGTGCCAGATAAACGTCTGGCACGAA
TCGATGAATTGATTCCGGCCAAGAAGATTATTCATACAACTTTTGAATTCACAGATATTGCCGGCATTGTTAAAGGTGCT
TCTAAGGGTGAAGGACTCGGCAACAAGTTCCTTGAAAACATTCGTCAGGTGGATGCGATTGTTCATGTTGTCCGAGCGTT
CGATGATGACAACATCACCAGTGTAACTGGCAAAGTCGACCCGTTAGAGGATATGGCGACGATTAATATGGAATTGGCCA
TCGCTGATCTTGATGCTGTCAATAAACGCTATGCTAAAGTTGAAAAAGTGGCGCGAACACAAACCAATGACAAAGAAGCG
CAGGCTGAATTCAAGGTGCTCAAAAAGATTAAGCCCGTACTGGAAGATGGCGGGATGGTTCGCAGCATCGACTTTGATAA
AGATGAGCTGAAGATTGTCAAAGGCTTGTTCTTGCTGACCAGCAAGCCAGTTTTGTATGTCGCTAACATTGCTGAAGAAG
CGATGGCTGATCCGGATTCGGTTGATTACGTCAAGCAAATCAAGGCCGAGGCTGCCAAAGAAGGCGCCGAAGTGATTGCG
ATTTCTGCCAAAACTGAAGAAGAAATTGCTGAACTGGATGACGATGAAAAGGCAGACTTTTTGGCAGCAGAAGGTGTCGA
CGAGTCTGGGTTGGATAAGCTGATCAAGGCTAGCTATCATTTGCTGGGATTGGCAACCTTCTTCACAGCCGGTGGCAAAG
AAACCCGTGCGTGGACATTTAGACAGGGAATTAAGGCTCCAGAAGTTGCTGGGATTATTCATTCCGACTTCGAACGCGGT
TTTATTCGCGCCGAGACAATGTCCTTCACCGATTTAGACAAGTACGGATCTGTTCAGGCGGTTAAAGAAGCAGGCCGTTA
TCGATCCGAAGGCAAAGAATATGAAGTGCAGGATGGGGACATCATTGAATTCCGGTTCAATGTCTAA

Upstream 100 bases:

>100_bases
CATATTGTGATGATGTCCCGGCGCGATTTTGAAAAACGACTCAAAAAAGTGTTGGGTCATGTAGAGTAGACAGACTAAAT
AGAAAGTGTGAAGTAACCAA

Downstream 100 bases:

>100_bases
CGCATAACGACTTAGGAGATAGGAGTTTCCAATGGCAGAGAAACATGAAGCAGAGACGAAACAGACGGCAACCGATGCTG
CAGTAAATGGTAAAAATACT

Product: GTP-dependent nucleic acid-binding protein EngD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MALTAGIVGLPNVGKSTLFNAITKAGAEMANYPFATIDPNVGMVEVPDKRLARIDELIPAKKIIHTTFEFTDIAGIVKGA
SKGEGLGNKFLENIRQVDAIVHVVRAFDDDNITSVTGKVDPLEDMATINMELAIADLDAVNKRYAKVEKVARTQTNDKEA
QAEFKVLKKIKPVLEDGGMVRSIDFDKDELKIVKGLFLLTSKPVLYVANIAEEAMADPDSVDYVKQIKAEAAKEGAEVIA
ISAKTEEEIAELDDDEKADFLAAEGVDESGLDKLIKASYHLLGLATFFTAGGKETRAWTFRQGIKAPEVAGIIHSDFERG
FIRAETMSFTDLDKYGSVQAVKEAGRYRSEGKEYEVQDGDIIEFRFNV

Sequences:

>Translated_368_residues
MALTAGIVGLPNVGKSTLFNAITKAGAEMANYPFATIDPNVGMVEVPDKRLARIDELIPAKKIIHTTFEFTDIAGIVKGA
SKGEGLGNKFLENIRQVDAIVHVVRAFDDDNITSVTGKVDPLEDMATINMELAIADLDAVNKRYAKVEKVARTQTNDKEA
QAEFKVLKKIKPVLEDGGMVRSIDFDKDELKIVKGLFLLTSKPVLYVANIAEEAMADPDSVDYVKQIKAEAAKEGAEVIA
ISAKTEEEIAELDDDEKADFLAAEGVDESGLDKLIKASYHLLGLATFFTAGGKETRAWTFRQGIKAPEVAGIIHSDFERG
FIRAETMSFTDLDKYGSVQAVKEAGRYRSEGKEYEVQDGDIIEFRFNV
>Mature_367_residues
ALTAGIVGLPNVGKSTLFNAITKAGAEMANYPFATIDPNVGMVEVPDKRLARIDELIPAKKIIHTTFEFTDIAGIVKGAS
KGEGLGNKFLENIRQVDAIVHVVRAFDDDNITSVTGKVDPLEDMATINMELAIADLDAVNKRYAKVEKVARTQTNDKEAQ
AEFKVLKKIKPVLEDGGMVRSIDFDKDELKIVKGLFLLTSKPVLYVANIAEEAMADPDSVDYVKQIKAEAAKEGAEVIAI
SAKTEEEIAELDDDEKADFLAAEGVDESGLDKLIKASYHLLGLATFFTAGGKETRAWTFRQGIKAPEVAGIIHSDFERGF
IRAETMSFTDLDKYGSVQAVKEAGRYRSEGKEYEVQDGDIIEFRFNV

Specific function: GTP-dependent nucleic acid-binding protein which may act as a translation factor [H]

COG id: COG0012

COG function: function code J; Predicted GTPase, probable translation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI58761500, Length=378, Percent_Identity=43.1216931216931, Blast_Score=288, Evalue=8e-78,
Organism=Homo sapiens, GI58761502, Length=226, Percent_Identity=41.5929203539823, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI24308117, Length=111, Percent_Identity=35.1351351351351, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1787454, Length=368, Percent_Identity=52.445652173913, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1789574, Length=127, Percent_Identity=30.7086614173228, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17509631, Length=368, Percent_Identity=42.6630434782609, Blast_Score=288, Evalue=4e-78,
Organism=Saccharomyces cerevisiae, GI6319499, Length=384, Percent_Identity=39.5833333333333, Blast_Score=249, Evalue=5e-67,
Organism=Saccharomyces cerevisiae, GI6321773, Length=382, Percent_Identity=34.8167539267016, Blast_Score=207, Evalue=3e-54,
Organism=Saccharomyces cerevisiae, GI6321649, Length=340, Percent_Identity=26.7647058823529, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321962, Length=161, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24640873, Length=368, Percent_Identity=41.0326086956522, Blast_Score=271, Evalue=7e-73,
Organism=Drosophila melanogaster, GI24640877, Length=368, Percent_Identity=41.0326086956522, Blast_Score=271, Evalue=7e-73,
Organism=Drosophila melanogaster, GI24640875, Length=368, Percent_Identity=41.0326086956522, Blast_Score=271, Evalue=7e-73,
Organism=Drosophila melanogaster, GI24640879, Length=327, Percent_Identity=39.1437308868502, Blast_Score=220, Evalue=9e-58,
Organism=Drosophila melanogaster, GI24585318, Length=171, Percent_Identity=30.4093567251462, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR004396
- InterPro:   IPR013029
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR012676
- InterPro:   IPR023192 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF06071 YchF-GTPase_C [H]

EC number: NA

Molecular weight: Translated: 40275; Mature: 40144

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALTAGIVGLPNVGKSTLFNAITKAGAEMANYPFATIDPNVGMVEVPDKRLARIDELIPA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCEEECCHHHHHHHHHHCCH
KKIIHTTFEFTDIAGIVKGASKGEGLGNKFLENIRQVDAIVHVVRAFDDDNITSVTGKVD
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCC
PLEDMATINMELAIADLDAVNKRYAKVEKVARTQTNDKEAQAEFKVLKKIKPVLEDGGMV
CHHHHHHEEEEEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEE
RSIDFDKDELKIVKGLFLLTSKPVLYVANIAEEAMADPDSVDYVKQIKAEAAKEGAEVIA
EEECCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEE
ISAKTEEEIAELDDDEKADFLAAEGVDESGLDKLIKASYHLLGLATFFTAGGKETRAWTF
EECCCHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
RQGIKAPEVAGIIHSDFERGFIRAETMSFTDLDKYGSVQAVKEAGRYRSEGKEYEVQDGD
HHCCCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHCCHHHHHHHHHHHHCCCCEECCCCC
IIEFRFNV
EEEEEECC
>Mature Secondary Structure 
ALTAGIVGLPNVGKSTLFNAITKAGAEMANYPFATIDPNVGMVEVPDKRLARIDELIPA
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCEEECCHHHHHHHHHHCCH
KKIIHTTFEFTDIAGIVKGASKGEGLGNKFLENIRQVDAIVHVVRAFDDDNITSVTGKVD
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCC
PLEDMATINMELAIADLDAVNKRYAKVEKVARTQTNDKEAQAEFKVLKKIKPVLEDGGMV
CHHHHHHEEEEEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEE
RSIDFDKDELKIVKGLFLLTSKPVLYVANIAEEAMADPDSVDYVKQIKAEAAKEGAEVIA
EEECCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEE
ISAKTEEEIAELDDDEKADFLAAEGVDESGLDKLIKASYHLLGLATFFTAGGKETRAWTF
EECCCHHHHHHCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
RQGIKAPEVAGIIHSDFERGFIRAETMSFTDLDKYGSVQAVKEAGRYRSEGKEYEVQDGD
HHCCCCCHHHHHHHHHHHCCCEEEECCCCHHHHHHCCHHHHHHHHHHHHCCCCEECCCCC
IIEFRFNV
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]