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The map label for this gene is manZ [H]

Identifier: 116493254

GI number: 116493254

Start: 1483896

End: 1484807

Strand: Reverse

Name: manZ [H]

Synonym: PEPE_1516

Alternate gene names: 116493254

Gene position: 1484807-1483896 (Counterclockwise)

Preceding gene: 116493255

Following gene: 116493253

Centisome position: 81.03

GC content: 39.14

Gene sequence:

>912_bases
ATGCAAGAAAAAGTTCAATTAACTAAAAAAGATCGTATTCATGTTTGGTTACGTTCAACATTCCTTCAAGGTTCATGGAA
CTACGAACGTATGCAAAATGGTGGTTGGGCATATACATTAATCCCAGCTCTTAAAAAGTTATACAAGACAAAAGAAGACC
GTGCCGCAGCTTTGACACGTCACATGGAATTCTTTAATACTCATCCATACTTAGCTGCACCTATCCTTGGTGTAACAATG
TCTCTTGAAGAAGAACGTGCTAATGGTGCTCCTATTGATGACGTTGCGATTCAAGGGGTTAAAGTTGGTATGATGGGACC
TCTTGCCGGTGTTGGTGATCCGGTATTCTGGTTTACAGTTAAGCCAATTATTGGTGCTTTAGCTGCTTCACTTGCTTTGG
GTGGTAACATCCTTGGACCAATTCTTTACTTCGTACTTTGGAATGCTATTCGTATGGGATTCACATGGTATACACAAGAA
TTTGGTTATAAAGCTGGTTCAAAGATTACAGAAGACATGTCTGGTGGTTTACTACAAGACGTTACTAAAGGTGCTTCAAT
CTTGGGTATGTTTATCCTTGGTTCATTGATTAACCGTTGGGTTACTGTTAAGTTCACACCAGTTGTTTCAACTGTTAAAC
AAAGTAAAGGTGCTTACATCGACTGGGCAAACCTTCCAAGTGGTGCTAAAGGAATTCAAGAAGCATTGCAACAACAAGCT
GCTGGTCTTTCATTAACTCCAGAAAAAGTTACAACATTGCAAGATAACTTGGATCAATTAATTCCAGGACTTGCTGGATT
ACTTCTAACACTTCTTTGCATGTGGTTACTTAAGAAGGGTGTTTCTCCTATCGTTGTTATCTTTGGTTTATTCATCGTTG
GTGTTGTACTTCACGTACTCCACGTTATGTAA

Upstream 100 bases:

>100_bases
CCCTTTCAAAAATGGGTGGTTCAAGTAACGGAGGCAACTCAGGTTCCGGTGATCCGCTCGGCGATATAATTGATAAATAC
TAAAAGGAGGCCAACAGAAA

Downstream 100 bases:

>100_bases
TAAACTTAAATTAAGTCTGGGCTATGGCCTAGGCTTTTTTTATTAAAATTTTTGGTGAAATGTACCTTAAGTATTATAAT
TGGATAAAAAGAAGAATGGA

Product: mannose-specific PTS system component IID

Products: protein histidine; sugar phosphate

Alternate protein names: EII-M-Man; EIID-Man; PTS system mannose-specific EIID component [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MQEKVQLTKKDRIHVWLRSTFLQGSWNYERMQNGGWAYTLIPALKKLYKTKEDRAAALTRHMEFFNTHPYLAAPILGVTM
SLEEERANGAPIDDVAIQGVKVGMMGPLAGVGDPVFWFTVKPIIGALAASLALGGNILGPILYFVLWNAIRMGFTWYTQE
FGYKAGSKITEDMSGGLLQDVTKGASILGMFILGSLINRWVTVKFTPVVSTVKQSKGAYIDWANLPSGAKGIQEALQQQA
AGLSLTPEKVTTLQDNLDQLIPGLAGLLLTLLCMWLLKKGVSPIVVIFGLFIVGVVLHVLHVM

Sequences:

>Translated_303_residues
MQEKVQLTKKDRIHVWLRSTFLQGSWNYERMQNGGWAYTLIPALKKLYKTKEDRAAALTRHMEFFNTHPYLAAPILGVTM
SLEEERANGAPIDDVAIQGVKVGMMGPLAGVGDPVFWFTVKPIIGALAASLALGGNILGPILYFVLWNAIRMGFTWYTQE
FGYKAGSKITEDMSGGLLQDVTKGASILGMFILGSLINRWVTVKFTPVVSTVKQSKGAYIDWANLPSGAKGIQEALQQQA
AGLSLTPEKVTTLQDNLDQLIPGLAGLLLTLLCMWLLKKGVSPIVVIFGLFIVGVVLHVLHVM
>Mature_303_residues
MQEKVQLTKKDRIHVWLRSTFLQGSWNYERMQNGGWAYTLIPALKKLYKTKEDRAAALTRHMEFFNTHPYLAAPILGVTM
SLEEERANGAPIDDVAIQGVKVGMMGPLAGVGDPVFWFTVKPIIGALAASLALGGNILGPILYFVLWNAIRMGFTWYTQE
FGYKAGSKITEDMSGGLLQDVTKGASILGMFILGSLINRWVTVKFTPVVSTVKQSKGAYIDWANLPSGAKGIQEALQQQA
AGLSLTPEKVTTLQDNLDQLIPGLAGLLLTLLCMWLLKKGVSPIVVIFGLFIVGVVLHVLHVM

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3716

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IID

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIID domain [H]

Homologues:

Organism=Escherichia coli, GI1788122, Length=291, Percent_Identity=48.4536082474227, Blast_Score=294, Evalue=4e-81,
Organism=Escherichia coli, GI1789529, Length=303, Percent_Identity=32.6732673267327, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004704
- InterPro:   IPR018405 [H]

Pfam domain/function: PF03613 EIID-AGA [H]

EC number: 2.7.1.69

Molecular weight: Translated: 33204; Mature: 33204

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS51108 PTS_EIID

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEKVQLTKKDRIHVWLRSTFLQGSWNYERMQNGGWAYTLIPALKKLYKTKEDRAAALTR
CCCCHHCCCCCHHHHHHHHHHHCCCCCHHHHCCCCCEEHHHHHHHHHHHCCHHHHHHHHH
HMEFFNTHPYLAAPILGVTMSLEEERANGAPIDDVAIQGVKVGMMGPLAGVGDPVFWFTV
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCEEECCCCCCCCCCCCHHHHHH
KPIIGALAASLALGGNILGPILYFVLWNAIRMGFTWYTQEFGYKAGSKITEDMSGGLLQD
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHH
VTKGASILGMFILGSLINRWVTVKFTPVVSTVKQSKGAYIDWANLPSGAKGIQEALQQQA
HHHHHHHHHHHHHHHHHHCEEEEEEHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHH
AGLSLTPEKVTTLQDNLDQLIPGLAGLLLTLLCMWLLKKGVSPIVVIFGLFIVGVVLHVL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
HVM
HHC
>Mature Secondary Structure
MQEKVQLTKKDRIHVWLRSTFLQGSWNYERMQNGGWAYTLIPALKKLYKTKEDRAAALTR
CCCCHHCCCCCHHHHHHHHHHHCCCCCHHHHCCCCCEEHHHHHHHHHHHCCHHHHHHHHH
HMEFFNTHPYLAAPILGVTMSLEEERANGAPIDDVAIQGVKVGMMGPLAGVGDPVFWFTV
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCEEECCCCCCCCCCCCHHHHHH
KPIIGALAASLALGGNILGPILYFVLWNAIRMGFTWYTQEFGYKAGSKITEDMSGGLLQD
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCHHHHH
VTKGASILGMFILGSLINRWVTVKFTPVVSTVKQSKGAYIDWANLPSGAKGIQEALQQQA
HHHHHHHHHHHHHHHHHHCEEEEEEHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHH
AGLSLTPEKVTTLQDNLDQLIPGLAGLLLTLLCMWLLKKGVSPIVVIFGLFIVGVVLHVL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
HVM
HHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]