Click here to switch to the map view.

The map label for this gene is yiaE [C]

Identifier: 116493122

GI number: 116493122

Start: 1365596

End: 1366543

Strand: Direct

Name: yiaE [C]

Synonym: PEPE_1377

Alternate gene names: 116493122

Gene position: 1365596-1366543 (Clockwise)

Preceding gene: 116493109

Following gene: 116493124

Centisome position: 74.53

GC content: 36.71

Gene sequence:

>948_bases
ATGAAAATTTATATATCCGACCAAATTTCTAAACAAATTAGGACTAATTTACCCAAAGACAATGTGACATACCTTAATAG
TTCGTCTATTACTAAGGCAGACTTAAAAGAAATCCAAGTTGCTTGGGGCTATGACCATCATGTGCAATCTCTATTAGATA
CTCCTCACCATCAGTTAAAATGGATTCAATCTTTTAGTGCTGGAATTGATTATTTTCCTAAACAACAACTTACAGATCAA
AAAATTACTTTAACCAATACTAGCGGTGTCCACAGCGAAGCCATTGCCAATACGGTTCTTTTATATGCCTTATACTTCAT
GCGCGATATGAATTTAGCTCTTAACATGCATCGCCAAAAGCAATGGGATGGTAGTCGCCATTATGAGGAACTAACCCTAC
TCTCAAATAAAAAATGGTTAATTTTCGGTACTGGTCACATTGGACAAGAAATTGCTCGAGAAGCTAAAGCTTTAGGTGGT
TATACTACCGGTGTTAACCATTCTGGTCATGCAGCTACTAATTTTGATGATACCGTAGCAGATAACGCTTATTCAAATAT
CATTCAAGATGCTGATATTATTATCAACATTCTCCCCTTAACTCCAGAAACTACTCATATGTTTGACCATCAATTCTTTA
ATCAATTAGAGCATTTATATCTATTTATTAATGTCGGTCGAGGACCATCAGTCGATACTTCTGCCTTAATGCAAGCTCTT
GATGATGGTCACGTTAAACATGCCGCATTAGATGTTTTCGAAGAAGAACCGCTCACTTCAGATAGTCCACTATGGGATTA
TCCACAAGTTTTAATTACACCGCATAATTCAGCAGTAAGCTCTGAGATGCTGCCAGGTGTTCAGGATGTGTTTATCCACA
ACTTACAAACCTTTCTAAAAGATGGGCATCCCAACACAAATATTGTTGATTTAACTCGTGGTTACTAA

Upstream 100 bases:

>100_bases
CTTCCCAGCGTTGGTTTTTCTTGTCATTGTTAAAGCTCCTTACTGTAAAAAGTATTCTATATATTATATCATTGAGGCGA
TTAATCAAAGGAGGATTAAG

Downstream 100 bases:

>100_bases
AAAACTTGGTACATAGTAATTTTAATAGACTCCCTAGAGTTGTTATTATTGCTACGTATCAAGTTTTTTTAATTTTTAAT
TTGATTAACTTTTTGTAAAC

Product: D-3-phosphoglycerate dehydrogenase

Products: 3-phosphohydroxypyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MKIYISDQISKQIRTNLPKDNVTYLNSSSITKADLKEIQVAWGYDHHVQSLLDTPHHQLKWIQSFSAGIDYFPKQQLTDQ
KITLTNTSGVHSEAIANTVLLYALYFMRDMNLALNMHRQKQWDGSRHYEELTLLSNKKWLIFGTGHIGQEIAREAKALGG
YTTGVNHSGHAATNFDDTVADNAYSNIIQDADIIINILPLTPETTHMFDHQFFNQLEHLYLFINVGRGPSVDTSALMQAL
DDGHVKHAALDVFEEEPLTSDSPLWDYPQVLITPHNSAVSSEMLPGVQDVFIHNLQTFLKDGHPNTNIVDLTRGY

Sequences:

>Translated_315_residues
MKIYISDQISKQIRTNLPKDNVTYLNSSSITKADLKEIQVAWGYDHHVQSLLDTPHHQLKWIQSFSAGIDYFPKQQLTDQ
KITLTNTSGVHSEAIANTVLLYALYFMRDMNLALNMHRQKQWDGSRHYEELTLLSNKKWLIFGTGHIGQEIAREAKALGG
YTTGVNHSGHAATNFDDTVADNAYSNIIQDADIIINILPLTPETTHMFDHQFFNQLEHLYLFINVGRGPSVDTSALMQAL
DDGHVKHAALDVFEEEPLTSDSPLWDYPQVLITPHNSAVSSEMLPGVQDVFIHNLQTFLKDGHPNTNIVDLTRGY
>Mature_315_residues
MKIYISDQISKQIRTNLPKDNVTYLNSSSITKADLKEIQVAWGYDHHVQSLLDTPHHQLKWIQSFSAGIDYFPKQQLTDQ
KITLTNTSGVHSEAIANTVLLYALYFMRDMNLALNMHRQKQWDGSRHYEELTLLSNKKWLIFGTGHIGQEIAREAKALGG
YTTGVNHSGHAATNFDDTVADNAYSNIIQDADIIINILPLTPETTHMFDHQFFNQLEHLYLFINVGRGPSVDTSALMQAL
DDGHVKHAALDVFEEEPLTSDSPLWDYPQVLITPHNSAVSSEMLPGVQDVFIHNLQTFLKDGHPNTNIVDLTRGY

Specific function: Unknown

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=292, Percent_Identity=27.0547945205479, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI23308577, Length=288, Percent_Identity=26.7361111111111, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI61743967, Length=239, Percent_Identity=25.1046025104602, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4557497, Length=239, Percent_Identity=25.1046025104602, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI87082289, Length=314, Percent_Identity=28.9808917197452, Blast_Score=121, Evalue=5e-29,
Organism=Escherichia coli, GI87081824, Length=175, Percent_Identity=31.4285714285714, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789279, Length=151, Percent_Identity=31.1258278145695, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1787645, Length=272, Percent_Identity=23.5294117647059, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=287, Percent_Identity=25.0871080139373, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324055, Length=250, Percent_Identity=23.6, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321253, Length=155, Percent_Identity=27.0967741935484, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24585516, Length=232, Percent_Identity=27.5862068965517, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI28574286, Length=245, Percent_Identity=28.1632653061224, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI19921140, Length=234, Percent_Identity=27.3504273504274, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI45551003, Length=329, Percent_Identity=25.531914893617, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24585514, Length=321, Percent_Identity=25.5451713395639, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28574282, Length=321, Percent_Identity=25.5451713395639, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI45552429, Length=315, Percent_Identity=26.031746031746, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28574284, Length=321, Percent_Identity=25.5451713395639, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28571528, Length=259, Percent_Identity=26.2548262548263, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.95

Molecular weight: Translated: 35597; Mature: 35597

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIYISDQISKQIRTNLPKDNVTYLNSSSITKADLKEIQVAWGYDHHVQSLLDTPHHQLK
CEEEECHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHEECCCHHHHHHHHHCCHHHHH
WIQSFSAGIDYFPKQQLTDQKITLTNTSGVHSEAIANTVLLYALYFMRDMNLALNMHRQK
HHHHHHCCCCCCCCHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QWDGSRHYEELTLLSNKKWLIFGTGHIGQEIAREAKALGGYTTGVNHSGHAATNFDDTVA
CCCCCCCHHHHHEECCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
DNAYSNIIQDADIIINILPLTPETTHMFDHQFFNQLEHLYLFINVGRGPSVDTSALMQAL
HHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHHH
DDGHVKHAALDVFEEEPLTSDSPLWDYPQVLITPHNSAVSSEMLPGVQDVFIHNLQTFLK
HCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHH
DGHPNTNIVDLTRGY
CCCCCCCEEECCCCC
>Mature Secondary Structure
MKIYISDQISKQIRTNLPKDNVTYLNSSSITKADLKEIQVAWGYDHHVQSLLDTPHHQLK
CEEEECHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHEECCCHHHHHHHHHCCHHHHH
WIQSFSAGIDYFPKQQLTDQKITLTNTSGVHSEAIANTVLLYALYFMRDMNLALNMHRQK
HHHHHHCCCCCCCCHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QWDGSRHYEELTLLSNKKWLIFGTGHIGQEIAREAKALGGYTTGVNHSGHAATNFDDTVA
CCCCCCCHHHHHEECCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHH
DNAYSNIIQDADIIINILPLTPETTHMFDHQFFNQLEHLYLFINVGRGPSVDTSALMQAL
HHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHCEEEEEEECCCCCCCCHHHHHHHH
DDGHVKHAALDVFEEEPLTSDSPLWDYPQVLITPHNSAVSSEMLPGVQDVFIHNLQTFLK
HCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCHHHHHHHCCCHHHHHHHHHHHHHH
DGHPNTNIVDLTRGY
CCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-phosphoglycerate; NAD+

Specific reaction: 3-phosphoglycerate + NAD+ = 3-phosphohydroxypyruvate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626 [H]