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The map label for this gene is yclK [H]

Identifier: 116493099

GI number: 116493099

Start: 1339494

End: 1340942

Strand: Reverse

Name: yclK [H]

Synonym: PEPE_1353

Alternate gene names: 116493099

Gene position: 1340942-1339494 (Counterclockwise)

Preceding gene: 116493100

Following gene: 116493097

Centisome position: 73.18

GC content: 36.16

Gene sequence:

>1449_bases
ATGAAATTGTTTTTTCAACAGATGATGGCCTTTTTATTAGTAACTCTGTCATCTTTATTGATCGTTGGATTGGTTTTTAT
TCAGTTTACTAAAGACTCAGTTTACCGCAACAAATGGCATTCCTTGCAAGAATATGCTGATAGTATTTTTCAGCAAGCAA
TTGTGTTAGATACAGAAACTGCTGATGTAAAGAGTGTACAAGTTGATCAGCTTCAAGGTGTGGAAAAACTGTTAGATAAT
CAACACATTCACTTTACAATCTACACTAATGATGGAAATACGATTAGTTATCCAAACCGTAATATAGCTTCTCGAATTAC
TAAAGCTCAGCGAAGAGAATTGAAAAATGGTGACACGGTCAAAACCAAATCACGCATGACTTTTGATAAGTCAAGCCGAC
AAGTTACCATGACAGAGATGGATAAGCCTTATTTTAATCGTAATGGTAATCTGGTTTGCGTGATTTCGATTGGATCACCA
TTAGCTAACGTACAGGCTGACATCGAGAAGGTTAAAAAGAATCTTTTCATGACGTTTGCCTTTGCGATTATTGTAGCAGT
GATTGCTTCATATTTCTTATCACGTTACTTAGTAAGGCGAATCCAAGCAATTCGAGAAGCTGCGCACAAGGTTACGGAAG
GAAACTTTAATGTTCAGTTAGTTTCTGGGCGTAAGGATGAAATTGGAGAATTGACCAACGATTTTAATATTATGACTGCT
TCCCTGAAAGAGTCTAATGATGAAATAAAACGCCAAGAAGAACGGCGACGAGCCTTTATGGCTGATGCAGCTCATGAAAT
GAGAACGCCATTGACAACTATCAACGGTATTTTAGAGGGGCTTGAGTATGATGCAATTCCTGAAGATAGTAAAGAGGAAA
GCGTTCATTTAATGCAACGAGAGACGAAGCGGCTTATTCGGTTAGTTAATGATAATTTGGATTATGAACGGTTGCGCACG
AATAAAATTAGTTTAAATCGTCATCAGATTAATCTAAAGCAAGCAACTCAAAGGATTAGTAAACAACTAGCTAAAAAGGC
CAAACTTGAAAATGATGTGATTATTATTGATATGGCTGATGATGTGATGGTATACGCTGATACAGATCGCTTAATTCAAA
TCTTATTTAACATTATTCAAAATGGAATTCAATTCACTCAAAATGGAAAAATTACTATTCGGGCCCAAAATGCTAAAGAT
GGTACTGATATCCAAATTAGCGATAATGGAATTGGTATGACAGAAGAGCAGATCAAAAATATCTGGGAAAGATACTATAA
GGCTGATGCATCAAGAATTCAAACCAAAGGTGAATCTGGATTGGGGATGGCAATTGTCCATGAATTAATGCAAGTTCATG
GAGGACAAATTACTGTCGAGAGTACTCCAGAAAAAGGAACAACTTTTAAATTACATTTTCCAGAGCAACAAAAAAAGCTT
GGCCATTAA

Upstream 100 bases:

>100_bases
TTCGCCAAAAATTAGAAGCAGTTGGTCCACAAGTGGTCCAAACGGTATGGGGAGTTGGCTATAAGTTTGATGATAGTAAA
ATCGATGAGGATTAGTTAAT

Downstream 100 bases:

>100_bases
GCCAAGCTTTTTTCATGGATCATCTTACCACATTGATGGTGTGTTGAACCATTCGATACGTTCCTTAACAGTGTTCTTGA
TAGCTTCTGTACCTGAAGCA

Product: Signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MKLFFQQMMAFLLVTLSSLLIVGLVFIQFTKDSVYRNKWHSLQEYADSIFQQAIVLDTETADVKSVQVDQLQGVEKLLDN
QHIHFTIYTNDGNTISYPNRNIASRITKAQRRELKNGDTVKTKSRMTFDKSSRQVTMTEMDKPYFNRNGNLVCVISIGSP
LANVQADIEKVKKNLFMTFAFAIIVAVIASYFLSRYLVRRIQAIREAAHKVTEGNFNVQLVSGRKDEIGELTNDFNIMTA
SLKESNDEIKRQEERRRAFMADAAHEMRTPLTTINGILEGLEYDAIPEDSKEESVHLMQRETKRLIRLVNDNLDYERLRT
NKISLNRHQINLKQATQRISKQLAKKAKLENDVIIIDMADDVMVYADTDRLIQILFNIIQNGIQFTQNGKITIRAQNAKD
GTDIQISDNGIGMTEEQIKNIWERYYKADASRIQTKGESGLGMAIVHELMQVHGGQITVESTPEKGTTFKLHFPEQQKKL
GH

Sequences:

>Translated_482_residues
MKLFFQQMMAFLLVTLSSLLIVGLVFIQFTKDSVYRNKWHSLQEYADSIFQQAIVLDTETADVKSVQVDQLQGVEKLLDN
QHIHFTIYTNDGNTISYPNRNIASRITKAQRRELKNGDTVKTKSRMTFDKSSRQVTMTEMDKPYFNRNGNLVCVISIGSP
LANVQADIEKVKKNLFMTFAFAIIVAVIASYFLSRYLVRRIQAIREAAHKVTEGNFNVQLVSGRKDEIGELTNDFNIMTA
SLKESNDEIKRQEERRRAFMADAAHEMRTPLTTINGILEGLEYDAIPEDSKEESVHLMQRETKRLIRLVNDNLDYERLRT
NKISLNRHQINLKQATQRISKQLAKKAKLENDVIIIDMADDVMVYADTDRLIQILFNIIQNGIQFTQNGKITIRAQNAKD
GTDIQISDNGIGMTEEQIKNIWERYYKADASRIQTKGESGLGMAIVHELMQVHGGQITVESTPEKGTTFKLHFPEQQKKL
GH
>Mature_482_residues
MKLFFQQMMAFLLVTLSSLLIVGLVFIQFTKDSVYRNKWHSLQEYADSIFQQAIVLDTETADVKSVQVDQLQGVEKLLDN
QHIHFTIYTNDGNTISYPNRNIASRITKAQRRELKNGDTVKTKSRMTFDKSSRQVTMTEMDKPYFNRNGNLVCVISIGSP
LANVQADIEKVKKNLFMTFAFAIIVAVIASYFLSRYLVRRIQAIREAAHKVTEGNFNVQLVSGRKDEIGELTNDFNIMTA
SLKESNDEIKRQEERRRAFMADAAHEMRTPLTTINGILEGLEYDAIPEDSKEESVHLMQRETKRLIRLVNDNLDYERLRT
NKISLNRHQINLKQATQRISKQLAKKAKLENDVIIIDMADDVMVYADTDRLIQILFNIIQNGIQFTQNGKITIRAQNAKD
GTDIQISDNGIGMTEEQIKNIWERYYKADASRIQTKGESGLGMAIVHELMQVHGGQITVESTPEKGTTFKLHFPEQQKKL
GH

Specific function: Could be member of the two-component regulatory system yclK/yclJ. Potentially phosphorylates yclJ [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788393, Length=292, Percent_Identity=27.7397260273973, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI1786600, Length=225, Percent_Identity=29.7777777777778, Blast_Score=119, Evalue=5e-28,
Organism=Escherichia coli, GI1789149, Length=357, Percent_Identity=25.7703081232493, Blast_Score=117, Evalue=2e-27,
Organism=Escherichia coli, GI1790436, Length=236, Percent_Identity=30.9322033898305, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI145693157, Length=246, Percent_Identity=24.7967479674797, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1788713, Length=265, Percent_Identity=26.4150943396226, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87081816, Length=236, Percent_Identity=24.1525423728814, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI48994928, Length=243, Percent_Identity=27.5720164609054, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1786912, Length=261, Percent_Identity=25.6704980842912, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786783, Length=300, Percent_Identity=26, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1790861, Length=326, Percent_Identity=24.8466257668712, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790346, Length=272, Percent_Identity=25.3676470588235, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788549, Length=252, Percent_Identity=27.7777777777778, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1789808, Length=356, Percent_Identity=21.9101123595506, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787894, Length=297, Percent_Identity=22.5589225589226, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790551, Length=311, Percent_Identity=21.5434083601286, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788279, Length=319, Percent_Identity=23.5109717868339, Blast_Score=62, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 55187; Mature: 55187

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFFQQMMAFLLVTLSSLLIVGLVFIQFTKDSVYRNKWHSLQEYADSIFQQAIVLDTET
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
ADVKSVQVDQLQGVEKLLDNQHIHFTIYTNDGNTISYPNRNIASRITKAQRRELKNGDTV
CCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEECCCCHHHHHHHHHHHHHCCCCCCE
KTKSRMTFDKSSRQVTMTEMDKPYFNRNGNLVCVISIGSPLANVQADIEKVKKNLFMTFA
EECCCCEECCCCCEEEEHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
FAIIVAVIASYFLSRYLVRRIQAIREAAHKVTEGNFNVQLVSGRKDEIGELTNDFNIMTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHCCCCCEEEE
SLKESNDEIKRQEERRRAFMADAAHEMRTPLTTINGILEGLEYDAIPEDSKEESVHLMQR
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
ETKRLIRLVNDNLDYERLRTNKISLNRHQINLKQATQRISKQLAKKAKLENDVIIIDMAD
HHHHHHHHHHCCCCHHHHHCCCEECCHHEECHHHHHHHHHHHHHHHHCCCCCEEEEEECC
DVMVYADTDRLIQILFNIIQNGIQFTQNGKITIRAQNAKDGTDIQISDNGIGMTEEQIKN
CEEEEECHHHHHHHHHHHHHHCCEEECCCEEEEEECCCCCCCEEEECCCCCCCCHHHHHH
IWERYYKADASRIQTKGESGLGMAIVHELMQVHGGQITVESTPEKGTTFKLHFPEQQKKL
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCHHHHCC
GH
CC
>Mature Secondary Structure
MKLFFQQMMAFLLVTLSSLLIVGLVFIQFTKDSVYRNKWHSLQEYADSIFQQAIVLDTET
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCC
ADVKSVQVDQLQGVEKLLDNQHIHFTIYTNDGNTISYPNRNIASRITKAQRRELKNGDTV
CCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEECCCCHHHHHHHHHHHHHCCCCCCE
KTKSRMTFDKSSRQVTMTEMDKPYFNRNGNLVCVISIGSPLANVQADIEKVKKNLFMTFA
EECCCCEECCCCCEEEEHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHH
FAIIVAVIASYFLSRYLVRRIQAIREAAHKVTEGNFNVQLVSGRKDEIGELTNDFNIMTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHCCCCCEEEE
SLKESNDEIKRQEERRRAFMADAAHEMRTPLTTINGILEGLEYDAIPEDSKEESVHLMQR
HHHCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
ETKRLIRLVNDNLDYERLRTNKISLNRHQINLKQATQRISKQLAKKAKLENDVIIIDMAD
HHHHHHHHHHCCCCHHHHHCCCEECCHHEECHHHHHHHHHHHHHHHHCCCCCEEEEEECC
DVMVYADTDRLIQILFNIIQNGIQFTQNGKITIRAQNAKDGTDIQISDNGIGMTEEQIKN
CEEEEECHHHHHHHHHHHHHHCCEEECCCEEEEEECCCCCCCEEEECCCCCCCCHHHHHH
IWERYYKADASRIQTKGESGLGMAIVHELMQVHGGQITVESTPEKGTTFKLHFPEQQKKL
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCHHHHCC
GH
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]