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The map label for this gene is murI

Identifier: 116493005

GI number: 116493005

Start: 1242760

End: 1243557

Strand: Reverse

Name: murI

Synonym: PEPE_1257

Alternate gene names: 116493005

Gene position: 1243557-1242760 (Counterclockwise)

Preceding gene: 116493006

Following gene: 116493004

Centisome position: 67.87

GC content: 38.47

Gene sequence:

>798_bases
ATGGATAATCGTCCAATTGGTTTTATGGATTCAGGCGTCGGTGGTCTGACAGTTGTCAAAACAGCTCAAAAACTGTTACC
TAATGAAGAAATTATCTTTATTGGAGATGAAGCAAGAATGCCGTATGGTCCTCGTCCCACAGCAGAAGTCGTTGAATTTT
CACGACAGATGGCGTCATTTTTAATGACTAAAAATATTAAGGCGCTAGTGATTGCATGTAATACTGCGACCAACGCGGCG
TTAGCGGTTTTACAAGCTGAATTACCCATCCCAGTAATTGGGGTGATTTTACCTGGCGCAATTGCAGCTAATAGGCAAAC
TAAAAATCAAAAAATTGGAGTTATTGCTACACTAGGCACAATTAAATCTGAGGCTTACCCAAAGGCTTTAGCTGAAATTA
ATACCAAATTACGCGCTTATCCGGTAGCATGCCAAGAATTTGTAGAAATTGCTGAAAAAAATGAACTTCATACAACGGCA
GCTCAAAAAGTTATGAATGAAAAACTAGCTGAGTTTAGGCAAGATCAAATTGATACTTTGATTTTAGGCTGCACTCATTT
TCCGCTTTTAGAAGAAGGTATCCAAGCAGCCGTTGGGCCTGATGTTACTTTAGTAGATCCAGGAGTGGAGACCGTTCACC
AATTAATTGAAATTTTAACAAAACAAGCGCTTCAACATGCAGAAGGTCCTAAAGCGCAGGATCAATATTATTCAACGGGT
AATATTAAGAATTTTGAAGAAATAGCGCGGACATTCTTAAATCAAGATCTAAGAGTTGAAGAAGTTAAAATTGACTAG

Upstream 100 bases:

>100_bases
TTTCTTTTTTTATAGAGTTAGGTGGGTAACATGCTCCCTTTTTGTACTGAAATTTAGTATTATGGATGTATGTATAATTT
TATTTAGTGGGGGAATTAAA

Downstream 100 bases:

>100_bases
GAGAAATTAGAATGAATAATGAAATTTTGATTGCCACGAAAAATGATGGAAAATTAAAAGAATTTAAACAAATTTTTGAA
CAAAAGGGCATTGTTGTAAA

Product: glutamate racemase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MDNRPIGFMDSGVGGLTVVKTAQKLLPNEEIIFIGDEARMPYGPRPTAEVVEFSRQMASFLMTKNIKALVIACNTATNAA
LAVLQAELPIPVIGVILPGAIAANRQTKNQKIGVIATLGTIKSEAYPKALAEINTKLRAYPVACQEFVEIAEKNELHTTA
AQKVMNEKLAEFRQDQIDTLILGCTHFPLLEEGIQAAVGPDVTLVDPGVETVHQLIEILTKQALQHAEGPKAQDQYYSTG
NIKNFEEIARTFLNQDLRVEEVKID

Sequences:

>Translated_265_residues
MDNRPIGFMDSGVGGLTVVKTAQKLLPNEEIIFIGDEARMPYGPRPTAEVVEFSRQMASFLMTKNIKALVIACNTATNAA
LAVLQAELPIPVIGVILPGAIAANRQTKNQKIGVIATLGTIKSEAYPKALAEINTKLRAYPVACQEFVEIAEKNELHTTA
AQKVMNEKLAEFRQDQIDTLILGCTHFPLLEEGIQAAVGPDVTLVDPGVETVHQLIEILTKQALQHAEGPKAQDQYYSTG
NIKNFEEIARTFLNQDLRVEEVKID
>Mature_265_residues
MDNRPIGFMDSGVGGLTVVKTAQKLLPNEEIIFIGDEARMPYGPRPTAEVVEFSRQMASFLMTKNIKALVIACNTATNAA
LAVLQAELPIPVIGVILPGAIAANRQTKNQKIGVIATLGTIKSEAYPKALAEINTKLRAYPVACQEFVEIAEKNELHTTA
AQKVMNEKLAEFRQDQIDTLILGCTHFPLLEEGIQAAVGPDVTLVDPGVETVHQLIEILTKQALQHAEGPKAQDQYYSTG
NIKNFEEIARTFLNQDLRVEEVKID

Specific function: Provides the (R)-glutamate required for cell wall biosynthesis

COG id: COG0796

COG function: function code M; Glutamate racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aspartate/glutamate racemases family

Homologues:

Organism=Escherichia coli, GI87082355, Length=231, Percent_Identity=32.034632034632, Blast_Score=98, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURI_PEDPA (Q03ES4)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804740.1
- ProteinModelPortal:   Q03ES4
- SMR:   Q03ES4
- STRING:   Q03ES4
- GeneID:   4417117
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1257
- NMPDR:   fig|278197.10.peg.1098
- eggNOG:   COG0796
- HOGENOM:   HBG645102
- OMA:   AILGCTH
- PhylomeDB:   Q03ES4
- ProtClustDB:   PRK00865
- BioCyc:   PPEN278197:PEPE_1257-MONOMER
- HAMAP:   MF_00258
- InterPro:   IPR015942
- InterPro:   IPR001920
- InterPro:   IPR018187
- InterPro:   IPR004391
- Gene3D:   G3DSA:3.40.50.1860
- TIGRFAMs:   TIGR00067

Pfam domain/function: PF01177 Asp_Glu_race; SSF53681 Asp/Glu_race

EC number: =5.1.1.3

Molecular weight: Translated: 28977; Mature: 28977

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00923 ASP_GLU_RACEMASE_1; PS00924 ASP_GLU_RACEMASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNRPIGFMDSGVGGLTVVKTAQKLLPNEEIIFIGDEARMPYGPRPTAEVVEFSRQMASF
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
LMTKNIKALVIACNTATNAALAVLQAELPIPVIGVILPGAIAANRQTKNQKIGVIATLGT
HHHCCCEEEEEEECCCCHHHHHHHHHCCCCCCHHHHHCCHHHCCCCCCCCCEEEEEEHHH
IKSEAYPKALAEINTKLRAYPVACQEFVEIAEKNELHTTAAQKVMNEKLAEFRQDQIDTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEE
ILGCTHFPLLEEGIQAAVGPDVTLVDPGVETVHQLIEILTKQALQHAEGPKAQDQYYSTG
EECCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
NIKNFEEIARTFLNQDLRVEEVKID
CCCCHHHHHHHHHCCCCEEEEEECC
>Mature Secondary Structure
MDNRPIGFMDSGVGGLTVVKTAQKLLPNEEIIFIGDEARMPYGPRPTAEVVEFSRQMASF
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
LMTKNIKALVIACNTATNAALAVLQAELPIPVIGVILPGAIAANRQTKNQKIGVIATLGT
HHHCCCEEEEEEECCCCHHHHHHHHHCCCCCCHHHHHCCHHHCCCCCCCCCEEEEEEHHH
IKSEAYPKALAEINTKLRAYPVACQEFVEIAEKNELHTTAAQKVMNEKLAEFRQDQIDTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEE
ILGCTHFPLLEEGIQAAVGPDVTLVDPGVETVHQLIEILTKQALQHAEGPKAQDQYYSTG
EECCCCCHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
NIKNFEEIARTFLNQDLRVEEVKID
CCCCHHHHHHHHHCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA