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The map label for this gene is iscS1 [H]

Identifier: 116492935

GI number: 116492935

Start: 1174306

End: 1175466

Strand: Reverse

Name: iscS1 [H]

Synonym: PEPE_1174

Alternate gene names: 116492935

Gene position: 1175466-1174306 (Counterclockwise)

Preceding gene: 116492936

Following gene: 116492934

Centisome position: 64.15

GC content: 37.38

Gene sequence:

>1161_bases
ATGCAACAAGTATATTTAGATAATGCAGCTACAACGCCAACGGATGCCGCGGTAGTTGAAGTAATATCTAATGAATTATT
AAATAACTGGGGTAATGCATCTAGTTTACATGGCTTCGGTCGTCGAGCTCGAGCTACACTTGACGAAGCTAGGCATATAA
TCGCACAAAGTATTCATGCGAATGATGATGAAATTATCTTTACTAGCGGTGGCACGGAGAGTAATAACACTGCCATTACA
CAAGTCGCTTTACGTAATCAAGATAAAGGTAAAAAAATTATTACTACTGCAATTGAACATCCATCAGTTTTGAACCCCAT
GCGTCGTTTAGAAAGTATGGGATTTGAAGTTACTTATTTAGGGGTCGATCAAAATGGATTGATTAGTTTGGATGAATTTA
AAAATGCGCTAGATGACCAAACAATCTTAGTAAGTATTATGACTGGTAACAATGAAGTTGGTAGCCATATGCCAATTCAT
GAAATTGGTGAAATATTGAAAAATCATCAAGCAGTATTGCATACCGATGCAGTACAAGCCTACGGGTTAGTAGATATTGA
TGTTAAGCGTGACCACATTGATTTATTATCAACTTCAGCCCACAAGTTGAACGGTCCAAAAATGATGGGCTTCTTGTATA
AAAACAACGAAGTTAATTTCGATAGCTTTATATTAGGCGGCGAACAAGAAGACAAGCGGCGTGCAGGTACAGAAAATGTT
GCCAATATTGCTGGATTTGCTAAGGCTGTTAGTTTGCTTGATGCAGACCAAAAGAATCAATTGCAGGCTCGTTATGCCGG
TTTTAAGCAAAAAATCATTCAAACTTTAAAAGAAAATGAAATTGAATTTGAAATCAATGGTTCGTTAGATGAAAACAACC
TCCAACATGTTTTTAACATTTGGTTGAAGGGAGTTTCAACTTACGTAATGCAAACTAACCTTGATCTTGCCGGAATCGCT
ATATCAGGTGGATCAGCTTGTACCGCTGGAAGCTTAGAACCATCACATGTATTGACGGCGATGTATGGTGAGGATTCACC
TCGCATTAGTGAATCGATTCGAATTAGCTTCGGTAAAAAGACTACGGAAGAAGATATTGAGTATCTGTGTGATCAGATTG
TAGCAATTGAACAACGATTAAAACAAATAAGCAATAAATAG

Upstream 100 bases:

>100_bases
GTGATGATGCGGATGTTTCTTTTGACGAATTTATTATTGACGCTGGAAAGCGCTCCGCATCAATGCTGATTGATTTATTT
AAGATGGAAGGGAATAAATA

Downstream 100 bases:

>100_bases
AAGCGAGGGGTTCAGATGGCTTTTACTAAAGAAGTGAATGTTAAGGGCGATAATGTCATTTACACGTTAAATGCTAATAT
AAAAAAATATACTTTATTAG

Product: cysteine sulfinate desulfinase/cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MQQVYLDNAATTPTDAAVVEVISNELLNNWGNASSLHGFGRRARATLDEARHIIAQSIHANDDEIIFTSGGTESNNTAIT
QVALRNQDKGKKIITTAIEHPSVLNPMRRLESMGFEVTYLGVDQNGLISLDEFKNALDDQTILVSIMTGNNEVGSHMPIH
EIGEILKNHQAVLHTDAVQAYGLVDIDVKRDHIDLLSTSAHKLNGPKMMGFLYKNNEVNFDSFILGGEQEDKRRAGTENV
ANIAGFAKAVSLLDADQKNQLQARYAGFKQKIIQTLKENEIEFEINGSLDENNLQHVFNIWLKGVSTYVMQTNLDLAGIA
ISGGSACTAGSLEPSHVLTAMYGEDSPRISESIRISFGKKTTEEDIEYLCDQIVAIEQRLKQISNK

Sequences:

>Translated_386_residues
MQQVYLDNAATTPTDAAVVEVISNELLNNWGNASSLHGFGRRARATLDEARHIIAQSIHANDDEIIFTSGGTESNNTAIT
QVALRNQDKGKKIITTAIEHPSVLNPMRRLESMGFEVTYLGVDQNGLISLDEFKNALDDQTILVSIMTGNNEVGSHMPIH
EIGEILKNHQAVLHTDAVQAYGLVDIDVKRDHIDLLSTSAHKLNGPKMMGFLYKNNEVNFDSFILGGEQEDKRRAGTENV
ANIAGFAKAVSLLDADQKNQLQARYAGFKQKIIQTLKENEIEFEINGSLDENNLQHVFNIWLKGVSTYVMQTNLDLAGIA
ISGGSACTAGSLEPSHVLTAMYGEDSPRISESIRISFGKKTTEEDIEYLCDQIVAIEQRLKQISNK
>Mature_386_residues
MQQVYLDNAATTPTDAAVVEVISNELLNNWGNASSLHGFGRRARATLDEARHIIAQSIHANDDEIIFTSGGTESNNTAIT
QVALRNQDKGKKIITTAIEHPSVLNPMRRLESMGFEVTYLGVDQNGLISLDEFKNALDDQTILVSIMTGNNEVGSHMPIH
EIGEILKNHQAVLHTDAVQAYGLVDIDVKRDHIDLLSTSAHKLNGPKMMGFLYKNNEVNFDSFILGGEQEDKRRAGTENV
ANIAGFAKAVSLLDADQKNQLQARYAGFKQKIIQTLKENEIEFEINGSLDENNLQHVFNIWLKGVSTYVMQTNLDLAGIA
ISGGSACTAGSLEPSHVLTAMYGEDSPRISESIRISFGKKTTEEDIEYLCDQIVAIEQRLKQISNK

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=390, Percent_Identity=37.9487179487179, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI156713448, Length=415, Percent_Identity=32.0481927710843, Blast_Score=154, Evalue=1e-37,
Organism=Escherichia coli, GI48994898, Length=388, Percent_Identity=39.6907216494845, Blast_Score=237, Evalue=9e-64,
Organism=Escherichia coli, GI1789175, Length=394, Percent_Identity=26.3959390862944, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787970, Length=401, Percent_Identity=23.1920199501247, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25143064, Length=389, Percent_Identity=37.7892030848329, Blast_Score=233, Evalue=1e-61,
Organism=Caenorhabditis elegans, GI17533177, Length=324, Percent_Identity=31.4814814814815, Blast_Score=134, Evalue=7e-32,
Organism=Saccharomyces cerevisiae, GI6319831, Length=385, Percent_Identity=39.7402597402597, Blast_Score=244, Evalue=2e-65,
Organism=Drosophila melanogaster, GI20129463, Length=396, Percent_Identity=38.3838383838384, Blast_Score=236, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42511; Mature: 42511

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQVYLDNAATTPTDAAVVEVISNELLNNWGNASSLHGFGRRARATLDEARHIIAQSIHA
CCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
NDDEIIFTSGGTESNNTAITQVALRNQDKGKKIITTAIEHPSVLNPMRRLESMGFEVTYL
CCCCEEEECCCCCCCCCEEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEE
GVDQNGLISLDEFKNALDDQTILVSIMTGNNEVGSHMPIHEIGEILKNHQAVLHTDAVQA
EECCCCCEEHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHCCHHEEEEHHHHH
YGLVDIDVKRDHIDLLSTSAHKLNGPKMMGFLYKNNEVNFDSFILGGEQEDKRRAGTENV
CCEEEEEECCCHHHHHHCCHHHCCCCEEEEEEEECCCCCHHHEEECCCCHHHHHCCCHHH
ANIAGFAKAVSLLDADQKNQLQARYAGFKQKIIQTLKENEIEFEINGSLDENNLQHVFNI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHH
WLKGVSTYVMQTNLDLAGIAISGGSACTAGSLEPSHVLTAMYGEDSPRISESIRISFGKK
HHHHHHHEEEEECCCEEEEEEECCCCCCCCCCCHHHEEEEEECCCCCCCCCEEEEEECCC
TTEEDIEYLCDQIVAIEQRLKQISNK
CCHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQQVYLDNAATTPTDAAVVEVISNELLNNWGNASSLHGFGRRARATLDEARHIIAQSIHA
CCEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
NDDEIIFTSGGTESNNTAITQVALRNQDKGKKIITTAIEHPSVLNPMRRLESMGFEVTYL
CCCCEEEECCCCCCCCCEEEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHCCCEEEEE
GVDQNGLISLDEFKNALDDQTILVSIMTGNNEVGSHMPIHEIGEILKNHQAVLHTDAVQA
EECCCCCEEHHHHHHHCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHCCHHEEEEHHHHH
YGLVDIDVKRDHIDLLSTSAHKLNGPKMMGFLYKNNEVNFDSFILGGEQEDKRRAGTENV
CCEEEEEECCCHHHHHHCCHHHCCCCEEEEEEEECCCCCHHHEEECCCCHHHHHCCCHHH
ANIAGFAKAVSLLDADQKNQLQARYAGFKQKIIQTLKENEIEFEINGSLDENNLQHVFNI
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHH
WLKGVSTYVMQTNLDLAGIAISGGSACTAGSLEPSHVLTAMYGEDSPRISESIRISFGKK
HHHHHHHEEEEECCCEEEEEEECCCCCCCCCCCHHHEEEEEECCCCCCCCCEEEEEECCC
TTEEDIEYLCDQIVAIEQRLKQISNK
CCHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]