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The map label for this gene is ywbA [H]

Identifier: 116491841

GI number: 116491841

Start: 23258

End: 24493

Strand: Reverse

Name: ywbA [H]

Synonym: PEPE_0023

Alternate gene names: 116491841

Gene position: 24493-23258 (Counterclockwise)

Preceding gene: 116491842

Following gene: 116491840

Centisome position: 1.34

GC content: 39.4

Gene sequence:

>1236_bases
ATGAATAAATTTATGGACTGGTTACAAAATGTCCTAATGCCACCACTTGCTAAAGCGGGGAATAATAAATATCTGGTTTC
CATTCGTAACGGACTAGTATTAACCCTACCGGCTGTGATTAGTGGTTCAATTTTCTTAATTATTGCTAATATTCCAATCC
CTGCCTGGACGAATTTTATGAAACCATATATGGACGCCATGGGAGTTGCAGTTAATGGTTCTTTCGGAATCATTTCATTG
TTAGCTGCCATCGGAATTGGTTACGAATTAGCTAAAGCTTTGGAGTTAGACGCTATCAATGGCGCTGGACTTTCCACGAT
GGCTTTTATCGTGGCTTCATTCAACAGTAAATACACCCTCGATACTGCAAACTTCGCTTCATCTGGCTTGTTTACAGCGA
TTGTTTGTTCATTTATTTCCGTTTTGATTTTGAACTTATTCGTTAAAAAAAATTGGGTTATTAAACTGCCAAATGGTGTT
CCAGCTGCTGTTAGCCAATCATTTGTTTCACTAATTCCTGGTTTTTTAGTTTTGCTATTATTCTGGGGGCTTCGAGTTCC
CCTTCATTTTGATATTAATCTATTTGTTCAAAGCATCTTCCATCCCCTATTATTTGCTATGAACAGTCTACCAGGAATTA
TGTTCTACACGTTATTAGTTAGTGTTTTGTGGGTAGCTGGTATCCATGGGGATATGACACTCGAAGGGATCTCGGATCCA
ATCTTCCTTCAATTTCTAACAGCTAATGGTATAGCTTACGCACACCATCAAGCTATACCATACTTAACATCATCTGGTTT
TTCTAGTTTATTTGTTAATGTTGGTGGAACCGGTGCAACCATTATGCTTGTATTTTGGATGCTTTCATCCAAGAGTAAGG
CTTATCGAGAACTTGGTAAAGTTGCCTTCCCCGGTGCCTTATTTGAAATTAATGAACCCGTAATTTTTGGTTTCCCAATA
GTTATGAATCCAATCACCTTGATTCCTTTTATTGTGATTCCTCAAATTTTAACGGTCCTAACCTACATTTTGATGAGCGT
CCATTTAGTTCGCCCAGCCGTAATGATGGTTCCTTGGACCATGCCACCAATTATCGGACCTTTAATGGCAACTGGTTGGG
ATTGGCGTGCAGGAGTTTGGTCAGCAATTGAACTAGTCATTGCCGGAGCAATCTACTATCCCTTCTTCAAAGTGGCTGAA
AAACAAATGGTTGCCCAAGAAGCAAGTGAGGCTTAA

Upstream 100 bases:

>100_bases
TGTGTCATTTTTTAGTTATATATCATTTGTAAGGGTTTACATTAATGATTTTTATCACTATACTGTTTTTGTTACCAAAA
TATAAAGGAGGAGATTTTTC

Downstream 100 bases:

>100_bases
TCTTTCATGAAAATCAAAATTCTATTAAAATATAGTGTAGTAGGTGCGATCTTGATTGCAGCTCTTGCTATTGCGATGAC
CCAACTTCATTTAACTTCAA

Product: cellobiose-specific PTS system IIC component

Products: pyruvate; diacetylchitobiose-6-phosphate [Cytoplasm]; cellobiose-6-phosphate [Cytoplasm] [C]

Alternate protein names: Putative PTS system EIIC component [H]

Number of amino acids: Translated: 411; Mature: 411

Protein sequence:

>411_residues
MNKFMDWLQNVLMPPLAKAGNNKYLVSIRNGLVLTLPAVISGSIFLIIANIPIPAWTNFMKPYMDAMGVAVNGSFGIISL
LAAIGIGYELAKALELDAINGAGLSTMAFIVASFNSKYTLDTANFASSGLFTAIVCSFISVLILNLFVKKNWVIKLPNGV
PAAVSQSFVSLIPGFLVLLLFWGLRVPLHFDINLFVQSIFHPLLFAMNSLPGIMFYTLLVSVLWVAGIHGDMTLEGISDP
IFLQFLTANGIAYAHHQAIPYLTSSGFSSLFVNVGGTGATIMLVFWMLSSKSKAYRELGKVAFPGALFEINEPVIFGFPI
VMNPITLIPFIVIPQILTVLTYILMSVHLVRPAVMMVPWTMPPIIGPLMATGWDWRAGVWSAIELVIAGAIYYPFFKVAE
KQMVAQEASEA

Sequences:

>Translated_411_residues
MNKFMDWLQNVLMPPLAKAGNNKYLVSIRNGLVLTLPAVISGSIFLIIANIPIPAWTNFMKPYMDAMGVAVNGSFGIISL
LAAIGIGYELAKALELDAINGAGLSTMAFIVASFNSKYTLDTANFASSGLFTAIVCSFISVLILNLFVKKNWVIKLPNGV
PAAVSQSFVSLIPGFLVLLLFWGLRVPLHFDINLFVQSIFHPLLFAMNSLPGIMFYTLLVSVLWVAGIHGDMTLEGISDP
IFLQFLTANGIAYAHHQAIPYLTSSGFSSLFVNVGGTGATIMLVFWMLSSKSKAYRELGKVAFPGALFEINEPVIFGFPI
VMNPITLIPFIVIPQILTVLTYILMSVHLVRPAVMMVPWTMPPIIGPLMATGWDWRAGVWSAIELVIAGAIYYPFFKVAE
KQMVAQEASEA
>Mature_411_residues
MNKFMDWLQNVLMPPLAKAGNNKYLVSIRNGLVLTLPAVISGSIFLIIANIPIPAWTNFMKPYMDAMGVAVNGSFGIISL
LAAIGIGYELAKALELDAINGAGLSTMAFIVASFNSKYTLDTANFASSGLFTAIVCSFISVLILNLFVKKNWVIKLPNGV
PAAVSQSFVSLIPGFLVLLLFWGLRVPLHFDINLFVQSIFHPLLFAMNSLPGIMFYTLLVSVLWVAGIHGDMTLEGISDP
IFLQFLTANGIAYAHHQAIPYLTSSGFSSLFVNVGGTGATIMLVFWMLSSKSKAYRELGKVAFPGALFEINEPVIFGFPI
VMNPITLIPFIVIPQILTVLTYILMSVHLVRPAVMMVPWTMPPIIGPLMATGWDWRAGVWSAIELVIAGAIYYPFFKVAE
KQMVAQEASEA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane [H]

COG id: COG1455

COG function: function code G; Phosphotransferase system cellobiose-specific component IIC

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-3 domain [H]

Homologues:

Organism=Escherichia coli, GI1788032, Length=444, Percent_Identity=29.954954954955, Blast_Score=187, Evalue=1e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003352
- InterPro:   IPR004796
- InterPro:   IPR004501 [H]

Pfam domain/function: PF02378 PTS_EIIC [H]

EC number: NA

Molecular weight: Translated: 44794; Mature: 44794

Theoretical pI: Translated: 8.38; Mature: 8.38

Prosite motif: PS51105 PTS_EIIC_TYPE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKFMDWLQNVLMPPLAKAGNNKYLVSIRNGLVLTLPAVISGSIFLIIANIPIPAWTNFM
CCHHHHHHHHHHCCCHHHCCCCEEEEEECCCEEEEEHHHHCCCEEEEEECCCCHHHHHHH
KPYMDAMGVAVNGSFGIISLLAAIGIGYELAKALELDAINGAGLSTMAFIVASFNSKYTL
HHHHHHCCEEECCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEE
DTANFASSGLFTAIVCSFISVLILNLFVKKNWVIKLPNGVPAAVSQSFVSLIPGFLVLLL
ECCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHH
FWGLRVPLHFDINLFVQSIFHPLLFAMNSLPGIMFYTLLVSVLWVAGIHGDMTLEGISDP
HHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCC
IFLQFLTANGIAYAHHQAIPYLTSSGFSSLFVNVGGTGATIMLVFWMLSSKSKAYRELGK
CEEEEECCCCEEEHHHCCCCCHHCCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHCC
VAFPGALFEINEPVIFGFPIVMNPITLIPFIVIPQILTVLTYILMSVHLVRPAVMMVPWT
CCCCCEEEEECCCEEECCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
MPPIIGPLMATGWDWRAGVWSAIELVIAGAIYYPFFKVAEKQMVAQEASEA
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKFMDWLQNVLMPPLAKAGNNKYLVSIRNGLVLTLPAVISGSIFLIIANIPIPAWTNFM
CCHHHHHHHHHHCCCHHHCCCCEEEEEECCCEEEEEHHHHCCCEEEEEECCCCHHHHHHH
KPYMDAMGVAVNGSFGIISLLAAIGIGYELAKALELDAINGAGLSTMAFIVASFNSKYTL
HHHHHHCCEEECCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEEE
DTANFASSGLFTAIVCSFISVLILNLFVKKNWVIKLPNGVPAAVSQSFVSLIPGFLVLLL
ECCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHH
FWGLRVPLHFDINLFVQSIFHPLLFAMNSLPGIMFYTLLVSVLWVAGIHGDMTLEGISDP
HHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEECCCCCC
IFLQFLTANGIAYAHHQAIPYLTSSGFSSLFVNVGGTGATIMLVFWMLSSKSKAYRELGK
CEEEEECCCCEEEHHHCCCCCHHCCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHCC
VAFPGALFEINEPVIFGFPIVMNPITLIPFIVIPQILTVLTYILMSVHLVRPAVMMVPWT
CCCCCEEEEECCCEEECCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
MPPIIGPLMATGWDWRAGVWSAIELVIAGAIYYPFFKVAEKQMVAQEASEA
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: diacetylchitobiose [Periplasm]; phosphoenolpyruvate; cellobiose [Periplasm] [C]

Specific reaction: phosphoenolpyruvate + diacetylchitobiose [Periplasm] = pyruvate + diacetylchitobiose-6-phosphate [Cytoplasm] phosphoenolpyruvate + cellobiose [Periplasm] = cellobiose-6-phosphate [Cytoplasm] + pyruvate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377 [H]