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The map label for this gene is gyrB [H]

Identifier: 116491823

GI number: 116491823

Start: 4284

End: 6230

Strand: Direct

Name: gyrB [H]

Synonym: PEPE_0005

Alternate gene names: 116491823

Gene position: 4284-6230 (Clockwise)

Preceding gene: 116491822

Following gene: 116491824

Centisome position: 0.23

GC content: 40.16

Gene sequence:

>1947_bases
TTGGCAGACGAAAAAGAAACGAAAGCAGAATTAGCCAGAGAATATGATGCGAGTCAAATTCAGGTTTTAGAGGGGCTCGA
AGCAGTTCGTAAACGCCCAGGGATGTATATTGGGTCGACTAGTTCTCAAGGACTACACCATTTGGTTTGGGAAATTATTG
ATAATGGTATTGATGAAGCTCTTGCAGGATTTGCAGACAAAATTGATGTGATCGTTGAAAAAGACAATAGCATTACCGTC
ACTGATAATGGACGTGGGATTCCGGTTGATATCCAAAAGAAAACTGGAAAACCAGCTTTAGAAACAGTCTTTACGGTCCT
ACATGCCGGAGGTAAATTCGGCGGTGGCGGTTATAAAGTTTCTGGAGGATTGCATGGTGTGGGTGCATCCGTTGTAAATG
CGTTATCAACGGAATTAGATGCGCGCGTCATGAAGGACGGTAAAATCTACTACATTGATTTTGCGCTAGGAAAAGTAAAA
ACACCGATGAAAACGATTGGTGATACTGAACATCCTGACGATCATGGAACTATTGTTCATTTCGTTCCAGATCCAGATAT
TTTCCAAGAAACTACCACATATGACATTAATATCTTAAAAACACGAATTCGTGAATTAGCCTTTTTGAACAAGGGTCTAC
GGATTACTTTGAAGGATATGCGTCCTGAAAAGCCAACTGAAGACGACTTCTTGTACGAAGGTGGGATTCGCCACTACGTT
GAATACTTAAACGAAGGCAAAGAAGTAATTTTCCCTGAACCTATCTATGTTGAAGGGGTTACAAAAGGTATCACTGTTGA
AGTAGCTATGCAATATATCGAAGGTTATCAAAGTAAATTGTTAACTTTTACTAACAATATTCATACCTACGAAGGCGGTA
CCCACGAAGAAGGTTTCAAACGTGCTTTAACACGAGTTATTAACGATTACGCTAAAAACAACAATATTTTAAAAGAAAAT
GATGATAAATTGTCTGGTGATGATGTTCGAGAAGGTTTGACGGCAGTAGTCAGCGTTAAGCATCCTGATCCTCAATTCGA
AGGACAAACGAAAACAAAATTGGGTAACTCAGATGCTCGGACAGCTGTTAACGAAGTGTTTGCTGAAACTTTCAATAAAT
TCTTATTGGAAAATCCTAAGGTTGCACGTCAAATTGTTGATAAGGGAATCTTGGCAGCAAAAGCACGAGTTGCCGCTAAA
CGAGCTCGTGAAGTTACGCGTAAGAAGAGTGGCCTAGAACTCAATAATCTTCCTGGTAAATTAGCTGATAATACTTCTAA
GGATCCTTCAATTAGTGAATTATTCATTGTCGAGGGTGATTCTGCCGGTGGTAGTGCTAAGTCCGGACGTTCGCGTCTCA
CACAAGCTATTTTGCCAATTCGTGGGAAGATTTTGAACGTTGAAAAAGCCACTTTGGATCGGGTTTTGGCCAATGAAGAA
ATTCGTTCACTCTTTACAGCGCTCGGAACTGGATTTGGTGAGGACTTTGATGTAAGTAAAGCCAACTATCATAAATTGAT
TATCATGACCGATGCCGATGTCGATGGTGCTCATATTCGGACACTATTATTGACGCTGTTCTATCGTTACATGCGTCCAA
TGATTGATGCAGGATTTGTTTACATTGCTCAACCACCGCTCTACCAAGTACGTCAAGGTAAGATGATCCAATATATCGAT
TCTGATGAAGAATTAGAAACAGTACTTGGACAATTATCACCATCACCAAAACCTGTAATTCAACGTTATAAAGGTCTTGG
TGAAATGGATGCTGAGCAACTTTGGGAAACAACCATGAATCCAGAAAATCGACGCTTGTTACGAGTTTCAGCCGAAGATG
CTGATGCTGCAAGTGGTGATTTTGAAATGTTGATGGGTGACAAGGTTGAACCACGTCGTAAATTCATTGAAGAGAACGCT
GTGTTTGTTAAAAACTTGGATATCTAA

Upstream 100 bases:

>100_bases
ACCACCAGCCTTAGTGGTGTTGCGCAACAATTAATTAATGAACCCCATGTCTTTAATATTGATCACGGAGTTTTAATGCA
GAGCAAGGAGGAATAAGCTT

Downstream 100 bases:

>100_bases
AAAGATTATTAAAAGTTAGACAGCCCAAAAAATAAGGGGGAAAACGAATTGTCAGAAGAATTGTCAGGACGAATTACAGA
TGTCAATTTAAGTAGTCAAA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 648; Mature: 647

Protein sequence:

>648_residues
MADEKETKAELAREYDASQIQVLEGLEAVRKRPGMYIGSTSSQGLHHLVWEIIDNGIDEALAGFADKIDVIVEKDNSITV
TDNGRGIPVDIQKKTGKPALETVFTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSTELDARVMKDGKIYYIDFALGKVK
TPMKTIGDTEHPDDHGTIVHFVPDPDIFQETTTYDINILKTRIRELAFLNKGLRITLKDMRPEKPTEDDFLYEGGIRHYV
EYLNEGKEVIFPEPIYVEGVTKGITVEVAMQYIEGYQSKLLTFTNNIHTYEGGTHEEGFKRALTRVINDYAKNNNILKEN
DDKLSGDDVREGLTAVVSVKHPDPQFEGQTKTKLGNSDARTAVNEVFAETFNKFLLENPKVARQIVDKGILAAKARVAAK
RAREVTRKKSGLELNNLPGKLADNTSKDPSISELFIVEGDSAGGSAKSGRSRLTQAILPIRGKILNVEKATLDRVLANEE
IRSLFTALGTGFGEDFDVSKANYHKLIIMTDADVDGAHIRTLLLTLFYRYMRPMIDAGFVYIAQPPLYQVRQGKMIQYID
SDEELETVLGQLSPSPKPVIQRYKGLGEMDAEQLWETTMNPENRRLLRVSAEDADAASGDFEMLMGDKVEPRRKFIEENA
VFVKNLDI

Sequences:

>Translated_648_residues
MADEKETKAELAREYDASQIQVLEGLEAVRKRPGMYIGSTSSQGLHHLVWEIIDNGIDEALAGFADKIDVIVEKDNSITV
TDNGRGIPVDIQKKTGKPALETVFTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSTELDARVMKDGKIYYIDFALGKVK
TPMKTIGDTEHPDDHGTIVHFVPDPDIFQETTTYDINILKTRIRELAFLNKGLRITLKDMRPEKPTEDDFLYEGGIRHYV
EYLNEGKEVIFPEPIYVEGVTKGITVEVAMQYIEGYQSKLLTFTNNIHTYEGGTHEEGFKRALTRVINDYAKNNNILKEN
DDKLSGDDVREGLTAVVSVKHPDPQFEGQTKTKLGNSDARTAVNEVFAETFNKFLLENPKVARQIVDKGILAAKARVAAK
RAREVTRKKSGLELNNLPGKLADNTSKDPSISELFIVEGDSAGGSAKSGRSRLTQAILPIRGKILNVEKATLDRVLANEE
IRSLFTALGTGFGEDFDVSKANYHKLIIMTDADVDGAHIRTLLLTLFYRYMRPMIDAGFVYIAQPPLYQVRQGKMIQYID
SDEELETVLGQLSPSPKPVIQRYKGLGEMDAEQLWETTMNPENRRLLRVSAEDADAASGDFEMLMGDKVEPRRKFIEENA
VFVKNLDI
>Mature_647_residues
ADEKETKAELAREYDASQIQVLEGLEAVRKRPGMYIGSTSSQGLHHLVWEIIDNGIDEALAGFADKIDVIVEKDNSITVT
DNGRGIPVDIQKKTGKPALETVFTVLHAGGKFGGGGYKVSGGLHGVGASVVNALSTELDARVMKDGKIYYIDFALGKVKT
PMKTIGDTEHPDDHGTIVHFVPDPDIFQETTTYDINILKTRIRELAFLNKGLRITLKDMRPEKPTEDDFLYEGGIRHYVE
YLNEGKEVIFPEPIYVEGVTKGITVEVAMQYIEGYQSKLLTFTNNIHTYEGGTHEEGFKRALTRVINDYAKNNNILKEND
DKLSGDDVREGLTAVVSVKHPDPQFEGQTKTKLGNSDARTAVNEVFAETFNKFLLENPKVARQIVDKGILAAKARVAAKR
AREVTRKKSGLELNNLPGKLADNTSKDPSISELFIVEGDSAGGSAKSGRSRLTQAILPIRGKILNVEKATLDRVLANEEI
RSLFTALGTGFGEDFDVSKANYHKLIIMTDADVDGAHIRTLLLTLFYRYMRPMIDAGFVYIAQPPLYQVRQGKMIQYIDS
DEELETVLGQLSPSPKPVIQRYKGLGEMDAEQLWETTMNPENRRLLRVSAEDADAASGDFEMLMGDKVEPRRKFIEENAV
FVKNLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=675, Percent_Identity=25.1851851851852, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI19913406, Length=642, Percent_Identity=24.7663551401869, Blast_Score=112, Evalue=8e-25,
Organism=Escherichia coli, GI48994957, Length=558, Percent_Identity=53.0465949820789, Blast_Score=556, Evalue=1e-159,
Organism=Escherichia coli, GI1789408, Length=635, Percent_Identity=40.7874015748031, Blast_Score=434, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI212645845, Length=543, Percent_Identity=26.1510128913444, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17535065, Length=661, Percent_Identity=24.6596066565809, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324241, Length=595, Percent_Identity=24.5378151260504, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI17136538, Length=634, Percent_Identity=24.4479495268139, Blast_Score=112, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 71964; Mature: 71832

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADEKETKAELAREYDASQIQVLEGLEAVRKRPGMYIGSTSSQGLHHLVWEIIDNGIDEA
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCHHHH
LAGFADKIDVIVEKDNSITVTDNGRGIPVDIQKKTGKPALETVFTVLHAGGKFGGGGYKV
HHHHHHCEEEEEECCCEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEE
SGGLHGVGASVVNALSTELDARVMKDGKIYYIDFALGKVKTPMKTIGDTEHPDDHGTIVH
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHCCCCCCCCCCCEEEE
FVPDPDIFQETTTYDINILKTRIRELAFLNKGLRITLKDMRPEKPTEDDFLYEGGIRHYV
ECCCCCHHHCCCCEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEECCCHHHHH
EYLNEGKEVIFPEPIYVEGVTKGITVEVAMQYIEGYQSKLLTFTNNIHTYEGGTHEEGFK
HHHHCCCEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHH
RALTRVINDYAKNNNILKENDDKLSGDDVREGLTAVVSVKHPDPQFEGQTKTKLGNSDAR
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCEEEEEECCCCCCCCCCHHHCCCCCHHH
TAVNEVFAETFNKFLLENPKVARQIVDKGILAAKARVAAKRAREVTRKKSGLELNNLPGK
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCC
LADNTSKDPSISELFIVEGDSAGGSAKSGRSRLTQAILPIRGKILNVEKATLDRVLANEE
CCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHH
IRSLFTALGTGFGEDFDVSKANYHKLIIMTDADVDGAHIRTLLLTLFYRYMRPMIDAGFV
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEE
YIAQPPLYQVRQGKMIQYIDSDEELETVLGQLSPSPKPVIQRYKGLGEMDAEQLWETTMN
EEECCCHHHHCCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHCC
PENRRLLRVSAEDADAASGDFEMLMGDKVEPRRKFIEENAVFVKNLDI
CCCCEEEEEECCCCCCCCCCHHHHCCCCCCHHHHHHHCCCEEEEECCC
>Mature Secondary Structure 
ADEKETKAELAREYDASQIQVLEGLEAVRKRPGMYIGSTSSQGLHHLVWEIIDNGIDEA
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHCCHHHH
LAGFADKIDVIVEKDNSITVTDNGRGIPVDIQKKTGKPALETVFTVLHAGGKFGGGGYKV
HHHHHHCEEEEEECCCEEEEECCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEE
SGGLHGVGASVVNALSTELDARVMKDGKIYYIDFALGKVKTPMKTIGDTEHPDDHGTIVH
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHCCCCCCCCCCCEEEE
FVPDPDIFQETTTYDINILKTRIRELAFLNKGLRITLKDMRPEKPTEDDFLYEGGIRHYV
ECCCCCHHHCCCCEEHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEECCCHHHHH
EYLNEGKEVIFPEPIYVEGVTKGITVEVAMQYIEGYQSKLLTFTNNIHTYEGGTHEEGFK
HHHHCCCEEECCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHH
RALTRVINDYAKNNNILKENDDKLSGDDVREGLTAVVSVKHPDPQFEGQTKTKLGNSDAR
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHCCCEEEEEECCCCCCCCCCHHHCCCCCHHH
TAVNEVFAETFNKFLLENPKVARQIVDKGILAAKARVAAKRAREVTRKKSGLELNNLPGK
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCC
LADNTSKDPSISELFIVEGDSAGGSAKSGRSRLTQAILPIRGKILNVEKATLDRVLANEE
CCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHH
IRSLFTALGTGFGEDFDVSKANYHKLIIMTDADVDGAHIRTLLLTLFYRYMRPMIDAGFV
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCEE
YIAQPPLYQVRQGKMIQYIDSDEELETVLGQLSPSPKPVIQRYKGLGEMDAEQLWETTMN
EEECCCHHHHCCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCHHHHHHHHCC
PENRRLLRVSAEDADAASGDFEMLMGDKVEPRRKFIEENAVFVKNLDI
CCCCEEEEEECCCCCCCCCCHHHHCCCCCCHHHHHHHCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377 [H]