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The map label for this gene is recF

Identifier: 116491822

GI number: 116491822

Start: 3155

End: 4279

Strand: Direct

Name: recF

Synonym: PEPE_0004

Alternate gene names: 116491822

Gene position: 3155-4279 (Clockwise)

Preceding gene: 116491821

Following gene: 116491823

Centisome position: 0.17

GC content: 39.29

Gene sequence:

>1125_bases
ATGTATCTGAAGACACTGGAGTTACATAATTTTCGTAATTATGCTGATCTTGTTGTCGAGTTCGGTTCGGGTATCAATGT
TCTATTGGGTGAAAATGCACAAGGCAAGACTAATCTATTAGAATCTATTTATTTCTTAGCTTTGACTAGAAGTCACCGAA
CCAATAGTGATCGAGATTTGATTAGTTGGAAAACCAAAGCAGCGCGAGTATCAGGGAGTGTCCAAAAAGAACACACCGTT
ACACCGCTTGAAATCAATTTATCTTCCAAAGGGAAAAATGCCAAAGTAAATCACCTTGAGCAAAGCCGTTTATCGCAATA
TGTCGGCCAGCTTAATGTGATTTTGTTTGCGCCTGAGGATTTATCAATTGTAAAAGGATCTCCAGCAGTTCGCCGTAAGT
TCATTGATATGGAATTTGGACAGATGAGTTCGAAATATCTATATAACTCGGCGCAATATCGATCCGTTCTGAAACAGCGA
AACCAGTACATTAAACAGCTACAGTTCAATCCTAAGGGTGACCAAGTTTATTTAGATGTTTTAAGTGATCAGCTAGCAGC
CCATGGAGCTGAAATCATTTTCCAACGCATTCAATTCTTGAAGAAGTTGGAAAAATGGTCCCAAGAGGTGCATAAGGAAA
TCTCGCAGGGAAAAGAAAAATTATCTTTTCAATATGTTTCGCCGATCAGTAGTGATCAAGCCGATACTACTGAAAAAATC
TATGCAGCACTACAAGCCCTTTTCCAAAAGCAACGTGAAAAGGAGCTTCAACAAGGTAAAACTTTAGTGGGGCCACATCT
TGACGATGTACGATTTATGGTTAATGACAAAAACGTATCGACGTTTGGTTCACAGGGGCAACAACGAACCACGGCCCTGA
GTGTTAAATTAGCAGAAATTGATTTGATGAAAGAAGAAACCGGAGAATATCCGGTTCTTTTACTTGATGATGTGTTATCT
GAACTTGACGATAGTCGTCAAACACACCTGTTAACTGCCATTCAAAACAAAGTACAAACTTTCATCACGACCACCAGCCT
TAGTGGTGTTGCGCAACAATTAATTAATGAACCCCATGTCTTTAATATTGATCACGGAGTTTTAATGCAGAGCAAGGAGG
AATAA

Upstream 100 bases:

>100_bases
GAGAACACGATGATCGCCGTGGAAAAAAACTTTACGAAGATGATGTAGTTGAAGTTCCAGATGAAGGTTCTTTCAAGATT
ACAATGAAATCTGGTAACTA

Downstream 100 bases:

>100_bases
GCTTTTGGCAGACGAAAAAGAAACGAAAGCAGAATTAGCCAGAGAATATGATGCGAGTCAAATTCAGGTTTTAGAGGGGC
TCGAAGCAGTTCGTAAACGC

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MYLKTLELHNFRNYADLVVEFGSGINVLLGENAQGKTNLLESIYFLALTRSHRTNSDRDLISWKTKAARVSGSVQKEHTV
TPLEINLSSKGKNAKVNHLEQSRLSQYVGQLNVILFAPEDLSIVKGSPAVRRKFIDMEFGQMSSKYLYNSAQYRSVLKQR
NQYIKQLQFNPKGDQVYLDVLSDQLAAHGAEIIFQRIQFLKKLEKWSQEVHKEISQGKEKLSFQYVSPISSDQADTTEKI
YAALQALFQKQREKELQQGKTLVGPHLDDVRFMVNDKNVSTFGSQGQQRTTALSVKLAEIDLMKEETGEYPVLLLDDVLS
ELDDSRQTHLLTAIQNKVQTFITTTSLSGVAQQLINEPHVFNIDHGVLMQSKEE

Sequences:

>Translated_374_residues
MYLKTLELHNFRNYADLVVEFGSGINVLLGENAQGKTNLLESIYFLALTRSHRTNSDRDLISWKTKAARVSGSVQKEHTV
TPLEINLSSKGKNAKVNHLEQSRLSQYVGQLNVILFAPEDLSIVKGSPAVRRKFIDMEFGQMSSKYLYNSAQYRSVLKQR
NQYIKQLQFNPKGDQVYLDVLSDQLAAHGAEIIFQRIQFLKKLEKWSQEVHKEISQGKEKLSFQYVSPISSDQADTTEKI
YAALQALFQKQREKELQQGKTLVGPHLDDVRFMVNDKNVSTFGSQGQQRTTALSVKLAEIDLMKEETGEYPVLLLDDVLS
ELDDSRQTHLLTAIQNKVQTFITTTSLSGVAQQLINEPHVFNIDHGVLMQSKEE
>Mature_374_residues
MYLKTLELHNFRNYADLVVEFGSGINVLLGENAQGKTNLLESIYFLALTRSHRTNSDRDLISWKTKAARVSGSVQKEHTV
TPLEINLSSKGKNAKVNHLEQSRLSQYVGQLNVILFAPEDLSIVKGSPAVRRKFIDMEFGQMSSKYLYNSAQYRSVLKQR
NQYIKQLQFNPKGDQVYLDVLSDQLAAHGAEIIFQRIQFLKKLEKWSQEVHKEISQGKEKLSFQYVSPISSDQADTTEKI
YAALQALFQKQREKELQQGKTLVGPHLDDVRFMVNDKNVSTFGSQGQQRTTALSVKLAEIDLMKEETGEYPVLLLDDVLS
ELDDSRQTHLLTAIQNKVQTFITTTSLSGVAQQLINEPHVFNIDHGVLMQSKEE

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=384, Percent_Identity=23.4375, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_PEDPA (Q03I57)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_803557.1
- STRING:   Q03I57
- GeneID:   4417001
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_0004
- NMPDR:   fig|278197.10.peg.4
- eggNOG:   COG1195
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- PhylomeDB:   Q03I57
- ProtClustDB:   PRK00064
- BioCyc:   PPEN278197:PEPE_0004-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 42482; Mature: 42482

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLKTLELHNFRNYADLVVEFGSGINVLLGENAQGKTNLLESIYFLALTRSHRTNSDRDL
CCEEEEEHHCCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCHH
ISWKTKAARVSGSVQKEHTVTPLEINLSSKGKNAKVNHLEQSRLSQYVGQLNVILFAPED
HHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCC
LSIVKGSPAVRRKFIDMEFGQMSSKYLYNSAQYRSVLKQRNQYIKQLQFNPKGDQVYLDV
CEEECCCHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHH
LSDQLAAHGAEIIFQRIQFLKKLEKWSQEVHKEISQGKEKLSFQYVSPISSDQADTTEKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHH
YAALQALFQKQREKELQQGKTLVGPHLDDVRFMVNDKNVSTFGSQGQQRTTALSVKLAEI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHCCCCCCHHHHEEEEHHHH
DLMKEETGEYPVLLLDDVLSELDDSRQTHLLTAIQNKVQTFITTTSLSGVAQQLINEPHV
HHHHHHCCCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
FNIDHGVLMQSKEE
EECCCCCCCCCCCC
>Mature Secondary Structure
MYLKTLELHNFRNYADLVVEFGSGINVLLGENAQGKTNLLESIYFLALTRSHRTNSDRDL
CCEEEEEHHCCCHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCHH
ISWKTKAARVSGSVQKEHTVTPLEINLSSKGKNAKVNHLEQSRLSQYVGQLNVILFAPED
HHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEEECCCC
LSIVKGSPAVRRKFIDMEFGQMSSKYLYNSAQYRSVLKQRNQYIKQLQFNPKGDQVYLDV
CEEECCCHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHH
LSDQLAAHGAEIIFQRIQFLKKLEKWSQEVHKEISQGKEKLSFQYVSPISSDQADTTEKI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCCHHHHHH
YAALQALFQKQREKELQQGKTLVGPHLDDVRFMVNDKNVSTFGSQGQQRTTALSVKLAEI
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCHHCCCCCCHHHHEEEEHHHH
DLMKEETGEYPVLLLDDVLSELDDSRQTHLLTAIQNKVQTFITTTSLSGVAQQLINEPHV
HHHHHHCCCCCEEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCE
FNIDHGVLMQSKEE
EECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA