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The map label for this gene is acnA [H]

Identifier: 116332659

GI number: 116332659

Start: 272791

End: 275058

Strand: Reverse

Name: acnA [H]

Synonym: LBJ_4240

Alternate gene names: 116332659

Gene position: 275058-272791 (Counterclockwise)

Preceding gene: 116332660

Following gene: 116332656

Centisome position: 91.76

GC content: 46.43

Gene sequence:

>2268_bases
ATGGCATTCGATATAGAAATGATTCGCGCCCGATATGCTAAGATCGGGGATCTAGTTACAAAAGCGAGAAACGTCGTTGG
AAGACCTCTTACCCTTACTGAAAAGATTCTTTATTCCCACCTTTGGGAAGGTGAACCTAAAACAGGTTATGAAAAAGGAA
AGTCATACGTAGACTTCGCACCGGACAGAGTCGCAATGCAGGACGCTACGGCTCAGATGGCTCTTTTGCAGTTTATGTCT
GCGGGAAGAAACAAAGTTGCGGTTCCATCCACCGTTCACTGTGACCACTTGATCCAAGCAAAAGACGGTGCCGTCGAGGA
TTTAAAAACCGCTAATACGGTAAATAAAGAAGTTTACGACTTTCTTTCCTCCGTTTCCAACAAATATGGAATCGGTTTTT
GGAAACCCGGTGCGGGTATCATTCACCAAGTGGTTTTGGAGAATTACGCATTTCCCGGCGGGATGATGATTGGAACCGAT
TCTCACACCGTAAATGCGGGTGGCTTGGGTATGATCGCGATCGGAGTCGGCGGTGCCGATGCCGTGGACGTGATGGCCGG
TATGGCTTGGGAACTAAAATTTCCAAAACTCATCGGTGTAAAACTCACTGGTAAACTTTCCGGCTGGGCCTCCGCAAAAG
ACATCATCTTGAAAGTTGCAGGAATCCTTACCGTTAAAGGCGGAACCGGTGCGATTGTAGAATACTTCGGAGAAGGTGCG
GACAGTCTTTCCTGTACGGGTAAGGGAACGATCTGTAATATGGGCGCAGAGATAGGAGCCACAACTTCCGTGTTCGGTTA
CGATCGGAACATGAGGGAATATCTCTTAAAAACGAATCGTAAGGATGTCGCGGAACTGGCGGATAAAATCGTGGAACATT
TAAACGGAGACAAAGAGGTATATGCGGATCCCGCGAAATACTTTGACCAGTTGATCGAAATCAATCTTTCAGAACTCGAA
CCCTATATCAATGGACCTTTTACTCCGGATTTAGCGACTCCTCTTTCTAAATTCAAGGAAGCGGTTCAACAGAACGGTTG
GCCGACAAACTTGGAAGTAGGGCTCATCGGTTCCTGCACGAACTCTTCGTACGAAGATATTACCCGTGCGGCTTCCGTTG
CAAGGCAAGCGGCGGAAAAAAATCTGGAAGTCAAAGCGGAATACACTGTAACTCCGGGCTCGGAAATGATCCGTTATACG
ATTGAAAGAGACGGACTGATCAAAACCTTTTCCGATATCGGTGGAGTGGTTCTTGCGAATGCCTGCGGGCCTTGTATCGG
TCAGTGGAGTCGTCACACGAAGGATCCGGAAAGAAAGAATTCCATCATTACTTCGTTTAACAGAAATTTTGCGAAACGTA
ACGACGGTCTTGCGGGAACTCACGCTTTCGTTGCGTCACCCGAAATTGTGACCGCATTTGCGATTGCCGGAACGTTGGAT
TTTAATCCGATCACGGATTCCTTGAAGAGTAAAGATGGAAAAGAAGTAAAACTCGATCCTCCGACGGGTCTTGATTTCCC
TCCGAAAGGTTTTGACGTAAAGGACGCGGGTTTTATCGCTCCCGCTGCGGACGGATCGAAAGTAAATGTCGTGGTCGATC
GTCAATCGGATCGTCTGCAGTTACTTTTACCGTTTGCTCCTTGGGAAAAAGCAGATTTAAAGGGTTTAAAATTACTCATC
AAAGCGAAAGGGAAGTGTACCACCGATCATATTTCTATGGCGGGACCTTGGTTGAAGTATAGAGGTCACTTGGATAATAT
TTCCAATAACCTTTTGATCGGAGCGGTAAACGCTTTTAACGATAAGACCAATGAAGTGAAAAATCAACTTTCCGGGACTT
ACGAGCCTGTTCCTCAAACTGCAAGAGCATACAAAGCGAAAGGTATCGGTTCCATAGTTGTAGGAGACGAGAATTATGGA
GAGGGATCTTCAAGAGAGCACGCGGCGATGGAACCAAGACATCTTGGAGTCAGAGCGGTTCTCGTAAAATCTTTCGCGAG
AATTCACGAGACAAACCTGAAAAAGCAGGGGATGCTCGCGCTTACGTTTGTGGACAAAGCCGATTACGATAAGATTCAAG
AAGAGGACACAATCGACATTCTCGGTTTGACCTCGTTTCAAGAAGGAACTCCTTTGACTCTCGTGTTACATCACAAAGAC
GGTTCTTCCCAAGAGATTAAAGTAAATCATACATTCAATGCGCAACAGATCGCTTGGTTCAAAGCAGGCAGCGCGCTCAA
CCTAATCAGCGAGGAGCAGAAGAAATAG

Upstream 100 bases:

>100_bases
TTGCAACAGCATGATTTTATAACTTGTCCTATTCTTTCGATGAACTGGAAGGACAACTCGTATAAGGACAGGTTCATTCA
TCATCTAACGGAGAAAGACA

Downstream 100 bases:

>100_bases
TTGTGAGGTTTTGGAAGTAGATCAAACTTTTCCCGCATTCTAGGAGCGCGGGAAAAGTCCGACGTAAAAAATTTACTTCG
ATTTTTTTTTGGTTGGCCCC

Product: aconitate hydratase

Products: NA

Alternate protein names: Aconitase; Citrate hydro-lyase [H]

Number of amino acids: Translated: 755; Mature: 754

Protein sequence:

>755_residues
MAFDIEMIRARYAKIGDLVTKARNVVGRPLTLTEKILYSHLWEGEPKTGYEKGKSYVDFAPDRVAMQDATAQMALLQFMS
AGRNKVAVPSTVHCDHLIQAKDGAVEDLKTANTVNKEVYDFLSSVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTD
SHTVNAGGLGMIAIGVGGADAVDVMAGMAWELKFPKLIGVKLTGKLSGWASAKDIILKVAGILTVKGGTGAIVEYFGEGA
DSLSCTGKGTICNMGAEIGATTSVFGYDRNMREYLLKTNRKDVAELADKIVEHLNGDKEVYADPAKYFDQLIEINLSELE
PYINGPFTPDLATPLSKFKEAVQQNGWPTNLEVGLIGSCTNSSYEDITRAASVARQAAEKNLEVKAEYTVTPGSEMIRYT
IERDGLIKTFSDIGGVVLANACGPCIGQWSRHTKDPERKNSIITSFNRNFAKRNDGLAGTHAFVASPEIVTAFAIAGTLD
FNPITDSLKSKDGKEVKLDPPTGLDFPPKGFDVKDAGFIAPAADGSKVNVVVDRQSDRLQLLLPFAPWEKADLKGLKLLI
KAKGKCTTDHISMAGPWLKYRGHLDNISNNLLIGAVNAFNDKTNEVKNQLSGTYEPVPQTARAYKAKGIGSIVVGDENYG
EGSSREHAAMEPRHLGVRAVLVKSFARIHETNLKKQGMLALTFVDKADYDKIQEEDTIDILGLTSFQEGTPLTLVLHHKD
GSSQEIKVNHTFNAQQIAWFKAGSALNLISEEQKK

Sequences:

>Translated_755_residues
MAFDIEMIRARYAKIGDLVTKARNVVGRPLTLTEKILYSHLWEGEPKTGYEKGKSYVDFAPDRVAMQDATAQMALLQFMS
AGRNKVAVPSTVHCDHLIQAKDGAVEDLKTANTVNKEVYDFLSSVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTD
SHTVNAGGLGMIAIGVGGADAVDVMAGMAWELKFPKLIGVKLTGKLSGWASAKDIILKVAGILTVKGGTGAIVEYFGEGA
DSLSCTGKGTICNMGAEIGATTSVFGYDRNMREYLLKTNRKDVAELADKIVEHLNGDKEVYADPAKYFDQLIEINLSELE
PYINGPFTPDLATPLSKFKEAVQQNGWPTNLEVGLIGSCTNSSYEDITRAASVARQAAEKNLEVKAEYTVTPGSEMIRYT
IERDGLIKTFSDIGGVVLANACGPCIGQWSRHTKDPERKNSIITSFNRNFAKRNDGLAGTHAFVASPEIVTAFAIAGTLD
FNPITDSLKSKDGKEVKLDPPTGLDFPPKGFDVKDAGFIAPAADGSKVNVVVDRQSDRLQLLLPFAPWEKADLKGLKLLI
KAKGKCTTDHISMAGPWLKYRGHLDNISNNLLIGAVNAFNDKTNEVKNQLSGTYEPVPQTARAYKAKGIGSIVVGDENYG
EGSSREHAAMEPRHLGVRAVLVKSFARIHETNLKKQGMLALTFVDKADYDKIQEEDTIDILGLTSFQEGTPLTLVLHHKD
GSSQEIKVNHTFNAQQIAWFKAGSALNLISEEQKK
>Mature_754_residues
AFDIEMIRARYAKIGDLVTKARNVVGRPLTLTEKILYSHLWEGEPKTGYEKGKSYVDFAPDRVAMQDATAQMALLQFMSA
GRNKVAVPSTVHCDHLIQAKDGAVEDLKTANTVNKEVYDFLSSVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDS
HTVNAGGLGMIAIGVGGADAVDVMAGMAWELKFPKLIGVKLTGKLSGWASAKDIILKVAGILTVKGGTGAIVEYFGEGAD
SLSCTGKGTICNMGAEIGATTSVFGYDRNMREYLLKTNRKDVAELADKIVEHLNGDKEVYADPAKYFDQLIEINLSELEP
YINGPFTPDLATPLSKFKEAVQQNGWPTNLEVGLIGSCTNSSYEDITRAASVARQAAEKNLEVKAEYTVTPGSEMIRYTI
ERDGLIKTFSDIGGVVLANACGPCIGQWSRHTKDPERKNSIITSFNRNFAKRNDGLAGTHAFVASPEIVTAFAIAGTLDF
NPITDSLKSKDGKEVKLDPPTGLDFPPKGFDVKDAGFIAPAADGSKVNVVVDRQSDRLQLLLPFAPWEKADLKGLKLLIK
AKGKCTTDHISMAGPWLKYRGHLDNISNNLLIGAVNAFNDKTNEVKNQLSGTYEPVPQTARAYKAKGIGSIVVGDENYGE
GSSREHAAMEPRHLGVRAVLVKSFARIHETNLKKQGMLALTFVDKADYDKIQEEDTIDILGLTSFQEGTPLTLVLHHKDG
SSQEIKVNHTFNAQQIAWFKAGSALNLISEEQKK

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate [H]

COG id: COG1048

COG function: function code C; Aconitase A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=742, Percent_Identity=61.8598382749326, Blast_Score=923, Evalue=0.0,
Organism=Homo sapiens, GI8659555, Length=845, Percent_Identity=28.0473372781065, Blast_Score=197, Evalue=3e-50,
Organism=Homo sapiens, GI41352693, Length=767, Percent_Identity=23.9895697522816, Blast_Score=177, Evalue=5e-44,
Organism=Escherichia coli, GI1787531, Length=821, Percent_Identity=26.7965895249695, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI87081781, Length=755, Percent_Identity=23.4437086092715, Blast_Score=129, Evalue=5e-31,
Organism=Escherichia coli, GI1786259, Length=477, Percent_Identity=25.9958071278826, Blast_Score=117, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25149337, Length=741, Percent_Identity=63.1578947368421, Blast_Score=941, Evalue=0.0,
Organism=Caenorhabditis elegans, GI32564738, Length=686, Percent_Identity=64.2857142857143, Blast_Score=895, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25149342, Length=576, Percent_Identity=63.3680555555556, Blast_Score=748, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17568399, Length=813, Percent_Identity=26.6912669126691, Blast_Score=194, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6323335, Length=733, Percent_Identity=62.3465211459754, Blast_Score=930, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322261, Length=754, Percent_Identity=53.183023872679, Blast_Score=781, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320440, Length=435, Percent_Identity=27.3563218390805, Blast_Score=130, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6321429, Length=467, Percent_Identity=26.5524625267666, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI281365315, Length=740, Percent_Identity=60.9459459459459, Blast_Score=888, Evalue=0.0,
Organism=Drosophila melanogaster, GI17864292, Length=740, Percent_Identity=60.9459459459459, Blast_Score=888, Evalue=0.0,
Organism=Drosophila melanogaster, GI28571643, Length=740, Percent_Identity=59.5945945945946, Blast_Score=858, Evalue=0.0,
Organism=Drosophila melanogaster, GI161076999, Length=686, Percent_Identity=62.2448979591837, Blast_Score=846, Evalue=0.0,
Organism=Drosophila melanogaster, GI24645686, Length=834, Percent_Identity=27.6978417266187, Blast_Score=188, Evalue=1e-47,
Organism=Drosophila melanogaster, GI17137564, Length=850, Percent_Identity=26.7058823529412, Blast_Score=177, Evalue=3e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR006249
- InterPro:   IPR015934
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00330 Aconitase; PF00694 Aconitase_C [H]

EC number: =4.2.1.3 [H]

Molecular weight: Translated: 81959; Mature: 81828

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFDIEMIRARYAKIGDLVTKARNVVGRPLTLTEKILYSHLWEGEPKTGYEKGKSYVDFA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHCCCEECCC
PDRVAMQDATAQMALLQFMSAGRNKVAVPSTVHCDHLIQAKDGAVEDLKTANTVNKEVYD
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHH
FLSSVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMIAIGVGGAD
HHHHHCCCCCCCEECCCHHHHHHHHHHHCCCCCCEEEECCCCEECCCCEEEEEEECCCCH
AVDVMAGMAWELKFPKLIGVKLTGKLSGWASAKDIILKVAGILTVKGGTGAIVEYFGEGA
HHHHHHCCEEEECCCEEEEEEEEECCCCCCHHHHHHHEEEEEEEECCCCCHHHHHHCCCC
DSLSCTGKGTICNMGAEIGATTSVFGYDRNMREYLLKTNRKDVAELADKIVEHLNGDKEV
CCEEECCCCCEEECCCCCCCCEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHE
YADPAKYFDQLIEINLSELEPYINGPFTPDLATPLSKFKEAVQQNGWPTNLEVGLIGSCT
EECHHHHHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
NSSYEDITRAASVARQAAEKNLEVKAEYTVTPGSEMIRYTIERDGLIKTFSDIGGVVLAN
CCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHEEEEECCCCHHHHHHHCCCEEEEC
ACGPCIGQWSRHTKDPERKNSIITSFNRNFAKRNDGLAGTHAFVASPEIVTAFAIAGTLD
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHEEEEEEECCC
FNPITDSLKSKDGKEVKLDPPTGLDFPPKGFDVKDAGFIAPAADGSKVNVVVDRQSDRLQ
CCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCCEE
LLLPFAPWEKADLKGLKLLIKAKGKCTTDHISMAGPWLKYRGHLDNISNNLLIGAVNAFN
EEEECCCCCCCCCCCEEEEEECCCCCCCCCHHCCCCHHHCCCCCCCCCCCEEEEEEHHCC
DKTNEVKNQLSGTYEPVPQTARAYKAKGIGSIVVGDENYGEGSSREHAAMEPRHLGVRAV
CHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCHHCCHHHH
LVKSFARIHETNLKKQGMLALTFVDKADYDKIQEEDTIDILGLTSFQEGTPLTLVLHHKD
HHHHHHHHHHHCCCCCCCEEEEEECCCCHHHCCCCCCEEEEEECCCCCCCCEEEEEECCC
GSSQEIKVNHTFNAQQIAWFKAGSALNLISEEQKK
CCCCEEEEEECCCCCEEEEEECCCHHHHHHHHHCC
>Mature Secondary Structure 
AFDIEMIRARYAKIGDLVTKARNVVGRPLTLTEKILYSHLWEGEPKTGYEKGKSYVDFA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHCCCEECCC
PDRVAMQDATAQMALLQFMSAGRNKVAVPSTVHCDHLIQAKDGAVEDLKTANTVNKEVYD
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHH
FLSSVSNKYGIGFWKPGAGIIHQVVLENYAFPGGMMIGTDSHTVNAGGLGMIAIGVGGAD
HHHHHCCCCCCCEECCCHHHHHHHHHHHCCCCCCEEEECCCCEECCCCEEEEEEECCCCH
AVDVMAGMAWELKFPKLIGVKLTGKLSGWASAKDIILKVAGILTVKGGTGAIVEYFGEGA
HHHHHHCCEEEECCCEEEEEEEEECCCCCCHHHHHHHEEEEEEEECCCCCHHHHHHCCCC
DSLSCTGKGTICNMGAEIGATTSVFGYDRNMREYLLKTNRKDVAELADKIVEHLNGDKEV
CCEEECCCCCEEECCCCCCCCEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHE
YADPAKYFDQLIEINLSELEPYINGPFTPDLATPLSKFKEAVQQNGWPTNLEVGLIGSCT
EECHHHHHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCC
NSSYEDITRAASVARQAAEKNLEVKAEYTVTPGSEMIRYTIERDGLIKTFSDIGGVVLAN
CCCHHHHHHHHHHHHHHHHCCCEEEEEEEECCCHHHEEEEECCCCHHHHHHHCCCEEEEC
ACGPCIGQWSRHTKDPERKNSIITSFNRNFAKRNDGLAGTHAFVASPEIVTAFAIAGTLD
CCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHEEEEEEECCC
FNPITDSLKSKDGKEVKLDPPTGLDFPPKGFDVKDAGFIAPAADGSKVNVVVDRQSDRLQ
CCCHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEEECCCCCEE
LLLPFAPWEKADLKGLKLLIKAKGKCTTDHISMAGPWLKYRGHLDNISNNLLIGAVNAFN
EEEECCCCCCCCCCCEEEEEECCCCCCCCCHHCCCCHHHCCCCCCCCCCCEEEEEEHHCC
DKTNEVKNQLSGTYEPVPQTARAYKAKGIGSIVVGDENYGEGSSREHAAMEPRHLGVRAV
CHHHHHHHHHCCCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCCHHCCHHHH
LVKSFARIHETNLKKQGMLALTFVDKADYDKIQEEDTIDILGLTSFQEGTPLTLVLHHKD
HHHHHHHHHHHCCCCCCCEEEEEECCCCHHHCCCCCCEEEEEECCCCCCCCEEEEEECCC
GSSQEIKVNHTFNAQQIAWFKAGSALNLISEEQKK
CCCCEEEEEECCCCCEEEEEECCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]