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The map label for this gene is rlmL [H]

Identifier: 116328937

GI number: 116328937

Start: 2758282

End: 2759226

Strand: Direct

Name: rlmL [H]

Synonym: LBL_2333

Alternate gene names: 116328937

Gene position: 2758282-2759226 (Clockwise)

Preceding gene: 116328936

Following gene: 116328938

Centisome position: 76.31

GC content: 45.5

Gene sequence:

>945_bases
ATGAAAACTGCAAAAAAAATCGAACCCCGGCGATCAAAAACTAACTCGCTAAAAAAGCCGGAACACACCTCGCAAAGCCA
CAACGCAGGGATCGAAACAGGTTCCTATAAACTACTCGATTCCGGGGACTTTCAAAAACTCGAACAAATCGGTCCTTATA
CGATCATTCGCCCTTCTCCCGTTTCTGCCTGGCCCCCAACAAAGCCGAATTTATGGGGAAACGCCGACGGGGAATACGTC
CGCTCGGACAAGGGCAGCGGGACCTGGAACTGGCGCAAAAAAGTAGACGAAGAATTTTACATTCAAATTTTAGAATATTC
TATTAAAATAAAATTCACTCCTTTTGGACACTTGGGAATTTTCGCCGAGCAGCTGGAAAACTGGAAGAAAATCCAACAGC
TTTCCTCCGGACTAAACAAGGAAGAGGAAGTGTTGAATCTCTTCGCGTATTCCGGAATTTCCACCTTGGCAGTGTTAGAC
GGAGGAGCTTCGGTTTGTCATCTGGATTCCTCCAAAGGAATGGTAGACTGGGCTCGGGACAACGCAAGCGTTTCCGGTCT
AGCGGATAAAAAGGTCCGTTGGATGGTGGAAGACGTTTTGAAGTTTTTAAAACGGGAAATCAAAAGAGGCAAAGATTATC
GCGGTTTTATTTTGGATCCTCCCACATTCGGAAGAGGTGCGAGCGGGGAAGTTTTTAAGATCGAAAAAGACCTTCCTGAA
CTCATGGATCTTCTTATGCAGCTCTGCGGAAACAAACCGGATTTTATCGTTCTTACCTGTCACTCCACGGGCTTTAGCCC
GTTAGCCCTTCAAAGAATTTTAGAAGGAAGAATCCGCAACAAAGGTCGTTTTTATCTTGGAGAACTTTCCATTCCGGAAG
AAAGCGGAAAACTTTACCCCGCCGGTTCGAATTGTATCTATGTTTCAGAGAGACTTTCGCTTTGA

Upstream 100 bases:

>100_bases
AAAGGCTGGACGTTTGAACGTTTTACGAGTTTAAAACCGGAAGCGGATCATCCGATAAGAAAGAATTTTCCGGAAGGAAA
TTATTTTAAGGTGCATATCT

Downstream 100 bases:

>100_bases
ACGAAGAAAAAGAAATCTCGTTTTTAGAGATCACCAGTTTTTCGAACGAGAAACTGAAACATATTTCCAATCTCAAAGAA
AAAAAACACAGGGAAACTTC

Product: SAM dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKTAKKIEPRRSKTNSLKKPEHTSQSHNAGIETGSYKLLDSGDFQKLEQIGPYTIIRPSPVSAWPPTKPNLWGNADGEYV
RSDKGSGTWNWRKKVDEEFYIQILEYSIKIKFTPFGHLGIFAEQLENWKKIQQLSSGLNKEEEVLNLFAYSGISTLAVLD
GGASVCHLDSSKGMVDWARDNASVSGLADKKVRWMVEDVLKFLKREIKRGKDYRGFILDPPTFGRGASGEVFKIEKDLPE
LMDLLMQLCGNKPDFIVLTCHSTGFSPLALQRILEGRIRNKGRFYLGELSIPEESGKLYPAGSNCIYVSERLSL

Sequences:

>Translated_314_residues
MKTAKKIEPRRSKTNSLKKPEHTSQSHNAGIETGSYKLLDSGDFQKLEQIGPYTIIRPSPVSAWPPTKPNLWGNADGEYV
RSDKGSGTWNWRKKVDEEFYIQILEYSIKIKFTPFGHLGIFAEQLENWKKIQQLSSGLNKEEEVLNLFAYSGISTLAVLD
GGASVCHLDSSKGMVDWARDNASVSGLADKKVRWMVEDVLKFLKREIKRGKDYRGFILDPPTFGRGASGEVFKIEKDLPE
LMDLLMQLCGNKPDFIVLTCHSTGFSPLALQRILEGRIRNKGRFYLGELSIPEESGKLYPAGSNCIYVSERLSL
>Mature_314_residues
MKTAKKIEPRRSKTNSLKKPEHTSQSHNAGIETGSYKLLDSGDFQKLEQIGPYTIIRPSPVSAWPPTKPNLWGNADGEYV
RSDKGSGTWNWRKKVDEEFYIQILEYSIKIKFTPFGHLGIFAEQLENWKKIQQLSSGLNKEEEVLNLFAYSGISTLAVLD
GGASVCHLDSSKGMVDWARDNASVSGLADKKVRWMVEDVLKFLKREIKRGKDYRGFILDPPTFGRGASGEVFKIEKDLPE
LMDLLMQLCGNKPDFIVLTCHSTGFSPLALQRILEGRIRNKGRFYLGELSIPEESGKLYPAGSNCIYVSERLSL

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 35354; Mature: 35354

Theoretical pI: Translated: 9.21; Mature: 9.21

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTAKKIEPRRSKTNSLKKPEHTSQSHNAGIETGSYKLLDSGDFQKLEQIGPYTIIRPSP
CCCCHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHCCCEEEECCCC
VSAWPPTKPNLWGNADGEYVRSDKGSGTWNWRKKVDEEFYIQILEYSIKIKFTPFGHLGI
CCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHCCHHHHHEEEEEEEEEEECCCCHHHH
FAEQLENWKKIQQLSSGLNKEEEVLNLFAYSGISTLAVLDGGASVCHLDSSKGMVDWARD
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCEEECCC
NASVSGLADKKVRWMVEDVLKFLKREIKRGKDYRGFILDPPTFGRGASGEVFKIEKDLPE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCEEEEHHHHHH
LMDLLMQLCGNKPDFIVLTCHSTGFSPLALQRILEGRIRNKGRFYLGELSIPEESGKLYP
HHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEE
AGSNCIYVSERLSL
CCCCEEEECCCCCC
>Mature Secondary Structure
MKTAKKIEPRRSKTNSLKKPEHTSQSHNAGIETGSYKLLDSGDFQKLEQIGPYTIIRPSP
CCCCHHCCCCHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHCCCEEEECCCC
VSAWPPTKPNLWGNADGEYVRSDKGSGTWNWRKKVDEEFYIQILEYSIKIKFTPFGHLGI
CCCCCCCCCCCCCCCCCCEEECCCCCCCCHHHHHCCHHHHHEEEEEEEEEEECCCCHHHH
FAEQLENWKKIQQLSSGLNKEEEVLNLFAYSGISTLAVLDGGASVCHLDSSKGMVDWARD
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCCEEEECCCCCCEEECCC
NASVSGLADKKVRWMVEDVLKFLKREIKRGKDYRGFILDPPTFGRGASGEVFKIEKDLPE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCEEEEHHHHHH
LMDLLMQLCGNKPDFIVLTCHSTGFSPLALQRILEGRIRNKGRFYLGELSIPEESGKLYP
HHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEEE
AGSNCIYVSERLSL
CCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA