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The map label for this gene is rlmI [H]

Identifier: 116328936

GI number: 116328936

Start: 2756775

End: 2758325

Strand: Direct

Name: rlmI [H]

Synonym: LBL_2332

Alternate gene names: 116328936

Gene position: 2756775-2758325 (Clockwise)

Preceding gene: 116328935

Following gene: 116328937

Centisome position: 76.27

GC content: 41.13

Gene sequence:

>1551_bases
CTGAACCTTCCTATGACAAACTTCCGGTTTCGCAGATACCAGCTGAACCGGAAAAGCGAACTTTCCATCCGACGTAAACA
TCCCTGGATCTTTAGCGGAAATCTTTCTTCGGCAACTTCCTCTTTTGTCAACGGACAGTGGTTGAGCCTTTTCTCTACGG
AGAATTTACCGGTAGCAACCGGAATTTATTCGAACATAGGCCTCGTCGCGATTCGGGTGATACAATTTTCACCTTCCTTT
TCCAAGGAAAAGATTCGAGAAAGTTTAATCCACGCCCTACAAAAAAGAAACGAACTTAGAAAAACCACTAACGCATATAG
AATTTTACACGGAGAGAACGACTTCTTTCCGGGAGTCACCATCGATCGATTAAACTCAACCTGGGTCGTTCGGATTTATT
CATCCTCCTTGCTTGGGTATGGACGATGGCTCGTTTGGAATCTCTACGATATATGCAAAAATACTAAATTAGGAGAACCA
CAACCAAAACGGATTTTATTCGATCCCACCGAAAAGATCGGAGACGACAAATTCGTTCTTCGAAGACACAATTCCGGAGA
TATATCGACAACTTACCTTTCTTCGGAATTTGGAATACCTAATTCTCCCCAAAGTTTCACCCCTTTCACGCCAAATCTCA
AAACCGATTTTTCAGAACCCTTACGGGATCCGGCTTTATCGGATTTTCCGCCAACATCAGAAAATGCTGTATTATTAAAA
TCGAATTTATCCGATACGACTAAACGGATCGGAGAGAGGCTTTGGAGAGGAAAGCGCGAACCGATCAAGATGAAAATCGA
CCCGACAAAAAACAACCGGAACTCCGTTAAACGAAATGATAAATCCGAAAAAAAAACGCTTCGAATCCGAGAGACGATCC
AACTTTACGGCATCCGATTTCCGGTTGAACTTCCGGGACAAAAGGGAGGAATCTTTTTAGACCTTAGGAACCTACGCAGA
TTTCTCCTCGAAAACAAAAAACTCAGCAAAGATAAAAACTGCCTTCATCTTTTTTCCCACACCGGACTCACATCGATTTG
TATGGAAGTTGCTGGGGCAACTTCCGTTCTTTCAGTGGACGGTTCGAAACAGGCTCTGGATTCTTTTCAAAGAGTTTTGA
GTTTAAAAAAAAATACAAGTTATTGTAAACATAGTTTCATCCAGAAAAATCTTTTTCAAGAATTGGAAGACATTTTAAGA
AACAAAACATTTGGGCTGATCGTAATCGATCCGCCCAATCTCACGCCGGACGCAAAATCAAGAAAAAACGCATTCAGATC
CTATTCTTATCTGTTCGGAAGTTATCTTGCATCTTTAGAAACAACGGGAACGATCATTCTTTGTTCTTGTTCCGGTAGAA
TTCGGTCGAAAGAATTGGAATCACTCGCTCAGGATATTCTAAAAGCAAAAGGCTGGACGTTTGAACGTTTTACGAGTTTA
AAACCGGAAGCGGATCATCCGATAAGAAAGAATTTTCCGGAAGGAAATTATTTTAAGGTGCATATCTATGAAAACTGCAA
AAAAAATCGAACCCCGGCGATCAAAAACTAA

Upstream 100 bases:

>100_bases
GGAAGGAAATGATTGAATCGACCGATATGAAAATCGGTCGTCCAAGACAGATCTACACAGGCGCCACCGAAACTTCCTAC
AAAGAGGCCAAGAAACAAAC

Downstream 100 bases:

>100_bases
CTCGCTAAAAAAGCCGGAACACACCTCGCAAAGCCACAACGCAGGGATCGAAACAGGTTCCTATAAACTACTCGATTCCG
GGGACTTTCAAAAACTCGAA

Product: SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MNLPMTNFRFRRYQLNRKSELSIRRKHPWIFSGNLSSATSSFVNGQWLSLFSTENLPVATGIYSNIGLVAIRVIQFSPSF
SKEKIRESLIHALQKRNELRKTTNAYRILHGENDFFPGVTIDRLNSTWVVRIYSSSLLGYGRWLVWNLYDICKNTKLGEP
QPKRILFDPTEKIGDDKFVLRRHNSGDISTTYLSSEFGIPNSPQSFTPFTPNLKTDFSEPLRDPALSDFPPTSENAVLLK
SNLSDTTKRIGERLWRGKREPIKMKIDPTKNNRNSVKRNDKSEKKTLRIRETIQLYGIRFPVELPGQKGGIFLDLRNLRR
FLLENKKLSKDKNCLHLFSHTGLTSICMEVAGATSVLSVDGSKQALDSFQRVLSLKKNTSYCKHSFIQKNLFQELEDILR
NKTFGLIVIDPPNLTPDAKSRKNAFRSYSYLFGSYLASLETTGTIILCSCSGRIRSKELESLAQDILKAKGWTFERFTSL
KPEADHPIRKNFPEGNYFKVHIYENCKKNRTPAIKN

Sequences:

>Translated_516_residues
MNLPMTNFRFRRYQLNRKSELSIRRKHPWIFSGNLSSATSSFVNGQWLSLFSTENLPVATGIYSNIGLVAIRVIQFSPSF
SKEKIRESLIHALQKRNELRKTTNAYRILHGENDFFPGVTIDRLNSTWVVRIYSSSLLGYGRWLVWNLYDICKNTKLGEP
QPKRILFDPTEKIGDDKFVLRRHNSGDISTTYLSSEFGIPNSPQSFTPFTPNLKTDFSEPLRDPALSDFPPTSENAVLLK
SNLSDTTKRIGERLWRGKREPIKMKIDPTKNNRNSVKRNDKSEKKTLRIRETIQLYGIRFPVELPGQKGGIFLDLRNLRR
FLLENKKLSKDKNCLHLFSHTGLTSICMEVAGATSVLSVDGSKQALDSFQRVLSLKKNTSYCKHSFIQKNLFQELEDILR
NKTFGLIVIDPPNLTPDAKSRKNAFRSYSYLFGSYLASLETTGTIILCSCSGRIRSKELESLAQDILKAKGWTFERFTSL
KPEADHPIRKNFPEGNYFKVHIYENCKKNRTPAIKN
>Mature_516_residues
MNLPMTNFRFRRYQLNRKSELSIRRKHPWIFSGNLSSATSSFVNGQWLSLFSTENLPVATGIYSNIGLVAIRVIQFSPSF
SKEKIRESLIHALQKRNELRKTTNAYRILHGENDFFPGVTIDRLNSTWVVRIYSSSLLGYGRWLVWNLYDICKNTKLGEP
QPKRILFDPTEKIGDDKFVLRRHNSGDISTTYLSSEFGIPNSPQSFTPFTPNLKTDFSEPLRDPALSDFPPTSENAVLLK
SNLSDTTKRIGERLWRGKREPIKMKIDPTKNNRNSVKRNDKSEKKTLRIRETIQLYGIRFPVELPGQKGGIFLDLRNLRR
FLLENKKLSKDKNCLHLFSHTGLTSICMEVAGATSVLSVDGSKQALDSFQRVLSLKKNTSYCKHSFIQKNLFQELEDILR
NKTFGLIVIDPPNLTPDAKSRKNAFRSYSYLFGSYLASLETTGTIILCSCSGRIRSKELESLAQDILKAKGWTFERFTSL
KPEADHPIRKNFPEGNYFKVHIYENCKKNRTPAIKN

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=209, Percent_Identity=28.7081339712919, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 59255; Mature: 59255

Theoretical pI: Translated: 10.53; Mature: 10.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLPMTNFRFRRYQLNRKSELSIRRKHPWIFSGNLSSATSSFVNGQWLSLFSTENLPVAT
CCCCCCCCEEEEEECCCCCCCCEEECCCEEEECCCCHHHHHHCCCCEEEEEECCCCCEEH
GIYSNIGLVAIRVIQFSPSFSKEKIRESLIHALQKRNELRKTTNAYRILHGENDFFPGVT
HHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCE
IDRLNSTWVVRIYSSSLLGYGRWLVWNLYDICKNTKLGEPQPKRILFDPTEKIGDDKFVL
EECCCCEEEEEEECCCHHHCCHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHCCCCEEEE
RRHNSGDISTTYLSSEFGIPNSPQSFTPFTPNLKTDFSEPLRDPALSDFPPTSENAVLLK
EECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEEE
SNLSDTTKRIGERLWRGKREPIKMKIDPTKNNRNSVKRNDKSEKKTLRIRETIQLYGIRF
CCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHHEEEEE
PVELPGQKGGIFLDLRNLRRFLLENKKLSKDKNCLHLFSHTGLTSICMEVAGATSVLSVD
CEECCCCCCCEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHCCCEEEEEC
GSKQALDSFQRVLSLKKNTSYCKHSFIQKNLFQELEDILRNKTFGLIVIDPPNLTPDAKS
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHH
RKNAFRSYSYLFGSYLASLETTGTIILCSCSGRIRSKELESLAQDILKAKGWTFERFTSL
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHCC
KPEADHPIRKNFPEGNYFKVHIYENCKKNRTPAIKN
CCCCCCCHHHCCCCCCEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MNLPMTNFRFRRYQLNRKSELSIRRKHPWIFSGNLSSATSSFVNGQWLSLFSTENLPVAT
CCCCCCCCEEEEEECCCCCCCCEEECCCEEEECCCCHHHHHHCCCCEEEEEECCCCCEEH
GIYSNIGLVAIRVIQFSPSFSKEKIRESLIHALQKRNELRKTTNAYRILHGENDFFPGVT
HHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCE
IDRLNSTWVVRIYSSSLLGYGRWLVWNLYDICKNTKLGEPQPKRILFDPTEKIGDDKFVL
EECCCCEEEEEEECCCHHHCCHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHCCCCEEEE
RRHNSGDISTTYLSSEFGIPNSPQSFTPFTPNLKTDFSEPLRDPALSDFPPTSENAVLLK
EECCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCEEEEE
SNLSDTTKRIGERLWRGKREPIKMKIDPTKNNRNSVKRNDKSEKKTLRIRETIQLYGIRF
CCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHHEEEEE
PVELPGQKGGIFLDLRNLRRFLLENKKLSKDKNCLHLFSHTGLTSICMEVAGATSVLSVD
CEECCCCCCCEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHCCHHHHHHHHHCCCEEEEEC
GSKQALDSFQRVLSLKKNTSYCKHSFIQKNLFQELEDILRNKTFGLIVIDPPNLTPDAKS
CCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHH
RKNAFRSYSYLFGSYLASLETTGTIILCSCSGRIRSKELESLAQDILKAKGWTFERFTSL
HHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHCC
KPEADHPIRKNFPEGNYFKVHIYENCKKNRTPAIKN
CCCCCCCHHHCCCCCCEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA