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The map label for this gene is mrdA [H]

Identifier: 116328600

GI number: 116328600

Start: 2285194

End: 2287140

Strand: Reverse

Name: mrdA [H]

Synonym: LBL_1957

Alternate gene names: 116328600

Gene position: 2287140-2285194 (Counterclockwise)

Preceding gene: 116328601

Following gene: 116328599

Centisome position: 63.28

GC content: 45.3

Gene sequence:

>1947_bases
TTGTTCGGCGGAGCGCAGTCTGCGTCGGAATACCGACTTGAAAAAAGTTTTCGTCTTCGTTTATATATTTTTTCCGGACT
TGTGGTGTTTACGCTCGCCGCGTTTATCTTTCAATTATTCAATCTACAGATTTTCAACGGGACGAGCAATGCACTCAAAG
CGGAAAAGTTCGTGAGAAAGAGCGAGACCATGCCGGCCGCGAGGGGGCAGATGTTCGATCGTAATTTTCTCACTCCGGAA
ACTTCCATGGCTCTCGTTTCGAATTATTCTTCCCTAGATGCGATCTTAAACACGTCCTTGTTCAAGTACGATCCCGCTTC
CGTAAAAGCGTTTATTCAGGAATTTGCACGGACACTTTCGATTCCGATCTCTTATTACGAGAACGAACTGATCGAGCCTC
GTTTTTCGAGAAACCTAAAGGCTAAAAAGCCGATCGTTCTTTTGGAGGGAATTACACATGCGCAACAGGAAAGAATATCA
GCGTTTGATAATATATCTAAATACATTATTCTCCTGCCTTCTCCTAGACGAATTTACAAAATGGGTCCGGCCCTCGCACA
CGTAACCGGTTATATCGGGAAACCGACTCGGGAAGATCTTCTTTCCGGGGAGATAAAATCGTATCAATATCTCGGAAAAA
GCGGACTCGAACTCGAATACGATTCCGAACTTCGAGGAGTGGACGGATTTAGGATTCAAAAAAGAAGTTCCGAAGGAAAC
ATAGAAGAAGAGCGCGTTGTGGAACATTCTTCACCCGGAAATAATTTGATCTTAACGATCGACAAAGACATTCAGATTGC
CGCTCACAAAGCGCTCAAGGGTAGTAGGGGAACTGCGATCGCGATCAAGGTCTCCACTGGAGAAATTCTCGCCATGGCTT
CCAACCCGAGTTACGATCCGAATATACTTTCCGGGAAAAATAAGGCGGATCGAACCCTGCATTTCAAACGGGTCAAAGAC
AATGGAGGGTTTTTAAATCTTGCGATCCAATCCAAGTTTCCTCCGGCTTCCACTTATAAAACGTTAGTTGCACTTGCGGC
ATTGGAGAGTCAACACAAGATCGGATATACTCCGGAAACGACGTTTTCTTGCAATGGAAGTTTTGTATTAAAATCCACGT
TCGCAGGAGTTCCCGATCAGGTTTTTAATTGTTGGGAAAAGGGAGGTCACGGAATAAATGATCTTGCACATGCTTTGCAG
AAATCCTGTTCGGTATATTTTTACAATCTTGGGTACAAACTCGGTTCCGACGCGATTTTAAACTACTCCAGGCTTTTCGG
ATTAGAAGACAAATCCAAAGTGGACCTTCCAGGAGAAATTACGGGTTTTGTCCCATCTTCTGCGTGGAAAAAAAGAACGT
ACGGGACCAAGTGGTTTGACGGGGATACGATCAATTTATCAATCGGGCAGGGGTTTATCTCAACTACACCGATGGGAATG
GCATTATTTTATATGGGACTTCTCAATCGTGGACAAATCTATCAGCCGTATGTGGTGAGTGAAATTCGAGATCCTGTAGA
TAACTCTATCATCAATAGAACCACACCCCAAATACTCAGAGATATTCCGATCAATCAGTCTTCTGCAGAAGCGATTAAAG
AAGGATTAAAGCTCGTGATCAAAAGTGGGACTGCGGCGTACGTTTTGAACAAGCCGTTCTTACCGGATATCGCAGGAAAA
ACGGGAACAGCTCAGACGAGAAGAAGAGGCTCTTCCGGATCCAACCATGCATGGTTTGTGGGATATGCACCCGCAAATGC
ACCTGTCAGCGAACAGGTATTAGTCGTAGTGTTTGTGGAATACGGAGTTGGAGGGGCGGCGGGAGCTGCTCCTGTGGCGA
GAGAAATGTTTCATGCCGCGTTTCCTCCGGGAACGTTCAAAAAATCACAGGATATAACGCCGTCTTCTCCTTCTACGGGA
CAGGAGGGAACGTTTGAAGCCAGATAA

Upstream 100 bases:

>100_bases
TCCTTATTCTTCACTTCGTTTTTTAATGCCCTCATAGCCCCGGCCTTCTTTTATGCCCTAACTTGGATCTATCAATTGGA
TCACATGGGGGACGCGTAAG

Downstream 100 bases:

>100_bases
GTCCATAGAGAGAATCGATTACTTTTTAGTGATTTCGGTCGTGATTGTCGTTCTTTGTAGCGTGATGACCCTATACAGTC
AGGAAGTCAACTTCGAAGAC

Product: cell division protein FtsI/penicillin-binding protein

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 648; Mature: 648

Protein sequence:

>648_residues
MFGGAQSASEYRLEKSFRLRLYIFSGLVVFTLAAFIFQLFNLQIFNGTSNALKAEKFVRKSETMPAARGQMFDRNFLTPE
TSMALVSNYSSLDAILNTSLFKYDPASVKAFIQEFARTLSIPISYYENELIEPRFSRNLKAKKPIVLLEGITHAQQERIS
AFDNISKYIILLPSPRRIYKMGPALAHVTGYIGKPTREDLLSGEIKSYQYLGKSGLELEYDSELRGVDGFRIQKRSSEGN
IEEERVVEHSSPGNNLILTIDKDIQIAAHKALKGSRGTAIAIKVSTGEILAMASNPSYDPNILSGKNKADRTLHFKRVKD
NGGFLNLAIQSKFPPASTYKTLVALAALESQHKIGYTPETTFSCNGSFVLKSTFAGVPDQVFNCWEKGGHGINDLAHALQ
KSCSVYFYNLGYKLGSDAILNYSRLFGLEDKSKVDLPGEITGFVPSSAWKKRTYGTKWFDGDTINLSIGQGFISTTPMGM
ALFYMGLLNRGQIYQPYVVSEIRDPVDNSIINRTTPQILRDIPINQSSAEAIKEGLKLVIKSGTAAYVLNKPFLPDIAGK
TGTAQTRRRGSSGSNHAWFVGYAPANAPVSEQVLVVVFVEYGVGGAAGAAPVAREMFHAAFPPGTFKKSQDITPSSPSTG
QEGTFEAR

Sequences:

>Translated_648_residues
MFGGAQSASEYRLEKSFRLRLYIFSGLVVFTLAAFIFQLFNLQIFNGTSNALKAEKFVRKSETMPAARGQMFDRNFLTPE
TSMALVSNYSSLDAILNTSLFKYDPASVKAFIQEFARTLSIPISYYENELIEPRFSRNLKAKKPIVLLEGITHAQQERIS
AFDNISKYIILLPSPRRIYKMGPALAHVTGYIGKPTREDLLSGEIKSYQYLGKSGLELEYDSELRGVDGFRIQKRSSEGN
IEEERVVEHSSPGNNLILTIDKDIQIAAHKALKGSRGTAIAIKVSTGEILAMASNPSYDPNILSGKNKADRTLHFKRVKD
NGGFLNLAIQSKFPPASTYKTLVALAALESQHKIGYTPETTFSCNGSFVLKSTFAGVPDQVFNCWEKGGHGINDLAHALQ
KSCSVYFYNLGYKLGSDAILNYSRLFGLEDKSKVDLPGEITGFVPSSAWKKRTYGTKWFDGDTINLSIGQGFISTTPMGM
ALFYMGLLNRGQIYQPYVVSEIRDPVDNSIINRTTPQILRDIPINQSSAEAIKEGLKLVIKSGTAAYVLNKPFLPDIAGK
TGTAQTRRRGSSGSNHAWFVGYAPANAPVSEQVLVVVFVEYGVGGAAGAAPVAREMFHAAFPPGTFKKSQDITPSSPSTG
QEGTFEAR
>Mature_648_residues
MFGGAQSASEYRLEKSFRLRLYIFSGLVVFTLAAFIFQLFNLQIFNGTSNALKAEKFVRKSETMPAARGQMFDRNFLTPE
TSMALVSNYSSLDAILNTSLFKYDPASVKAFIQEFARTLSIPISYYENELIEPRFSRNLKAKKPIVLLEGITHAQQERIS
AFDNISKYIILLPSPRRIYKMGPALAHVTGYIGKPTREDLLSGEIKSYQYLGKSGLELEYDSELRGVDGFRIQKRSSEGN
IEEERVVEHSSPGNNLILTIDKDIQIAAHKALKGSRGTAIAIKVSTGEILAMASNPSYDPNILSGKNKADRTLHFKRVKD
NGGFLNLAIQSKFPPASTYKTLVALAALESQHKIGYTPETTFSCNGSFVLKSTFAGVPDQVFNCWEKGGHGINDLAHALQ
KSCSVYFYNLGYKLGSDAILNYSRLFGLEDKSKVDLPGEITGFVPSSAWKKRTYGTKWFDGDTINLSIGQGFISTTPMGM
ALFYMGLLNRGQIYQPYVVSEIRDPVDNSIINRTTPQILRDIPINQSSAEAIKEGLKLVIKSGTAAYVLNKPFLPDIAGK
TGTAQTRRRGSSGSNHAWFVGYAPANAPVSEQVLVVVFVEYGVGGAAGAAPVAREMFHAAFPPGTFKKSQDITPSSPSTG
QEGTFEAR

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=638, Percent_Identity=27.4294670846395, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI1786272, Length=650, Percent_Identity=24.6153846153846, Blast_Score=125, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 71220; Mature: 71220

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGGAQSASEYRLEKSFRLRLYIFSGLVVFTLAAFIFQLFNLQIFNGTSNALKAEKFVRK
CCCCCCCHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHH
SETMPAARGQMFDRNFLTPETSMALVSNYSSLDAILNTSLFKYDPASVKAFIQEFARTLS
HCCCCCHHCCCCCCCCCCCCHHHHHHHCCHHHHHHHHCHHEECCHHHHHHHHHHHHHHHC
IPISYYENELIEPRFSRNLKAKKPIVLLEGITHAQQERISAFDNISKYIILLPSPRRIYK
CCHHHHHHHHCCCHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEECCCHHHHH
MGPALAHVTGYIGKPTREDLLSGEIKSYQYLGKSGLELEYDSELRGVDGFRIQKRSSEGN
HCHHHHHHHHCCCCCCHHHHHCCHHHHHHHHCCCCCCEEECCCCCCCCCEEEEECCCCCC
IEEERVVEHSSPGNNLILTIDKDIQIAAHKALKGSRGTAIAIKVSTGEILAMASNPSYDP
CCHHHHHHCCCCCCEEEEEECCCCEEHHHHHCCCCCCCEEEEEEECCEEEEEECCCCCCC
NILSGKNKADRTLHFKRVKDNGGFLNLAIQSKFPPASTYKTLVALAALESQHKIGYTPET
CCCCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
TFSCNGSFVLKSTFAGVPDQVFNCWEKGGHGINDLAHALQKSCSVYFYNLGYKLGSDAIL
EEECCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCEEEEEECCCCCCCHHHH
NYSRLFGLEDKSKVDLPGEITGFVPSSAWKKRTYGTKWFDGDTINLSIGQGFISTTPMGM
HHHHHCCCCCCCCCCCCCCEEECCCCHHHHHCCCCCCEECCCEEEEEECCCCCCCCCHHH
ALFYMGLLNRGQIYQPYVVSEIRDPVDNSIINRTTPQILRDIPINQSSAEAIKEGLKLVI
HHHHHHHCCCCCEECCHHHHHHHCHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHEEH
KSGTAAYVLNKPFLPDIAGKTGTAQTRRRGSSGSNHAWFVGYAPANAPVSEQVLVVVFVE
CCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEE
YGVGGAAGAAPVAREMFHAAFPPGTFKKSQDITPSSPSTGQEGTFEAR
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFGGAQSASEYRLEKSFRLRLYIFSGLVVFTLAAFIFQLFNLQIFNGTSNALKAEKFVRK
CCCCCCCHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHH
SETMPAARGQMFDRNFLTPETSMALVSNYSSLDAILNTSLFKYDPASVKAFIQEFARTLS
HCCCCCHHCCCCCCCCCCCCHHHHHHHCCHHHHHHHHCHHEECCHHHHHHHHHHHHHHHC
IPISYYENELIEPRFSRNLKAKKPIVLLEGITHAQQERISAFDNISKYIILLPSPRRIYK
CCHHHHHHHHCCCHHHCCCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEEECCCHHHHH
MGPALAHVTGYIGKPTREDLLSGEIKSYQYLGKSGLELEYDSELRGVDGFRIQKRSSEGN
HCHHHHHHHHCCCCCCHHHHHCCHHHHHHHHCCCCCCEEECCCCCCCCCEEEEECCCCCC
IEEERVVEHSSPGNNLILTIDKDIQIAAHKALKGSRGTAIAIKVSTGEILAMASNPSYDP
CCHHHHHHCCCCCCEEEEEECCCCEEHHHHHCCCCCCCEEEEEEECCEEEEEECCCCCCC
NILSGKNKADRTLHFKRVKDNGGFLNLAIQSKFPPASTYKTLVALAALESQHKIGYTPET
CCCCCCCCCCCEEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
TFSCNGSFVLKSTFAGVPDQVFNCWEKGGHGINDLAHALQKSCSVYFYNLGYKLGSDAIL
EEECCCCEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHCCEEEEEECCCCCCCHHHH
NYSRLFGLEDKSKVDLPGEITGFVPSSAWKKRTYGTKWFDGDTINLSIGQGFISTTPMGM
HHHHHCCCCCCCCCCCCCCEEECCCCHHHHHCCCCCCEECCCEEEEEECCCCCCCCCHHH
ALFYMGLLNRGQIYQPYVVSEIRDPVDNSIINRTTPQILRDIPINQSSAEAIKEGLKLVI
HHHHHHHCCCCCEECCHHHHHHHCHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHEEH
KSGTAAYVLNKPFLPDIAGKTGTAQTRRRGSSGSNHAWFVGYAPANAPVSEQVLVVVFVE
CCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEECCCCCCCCCCEEEEEEEE
YGVGGAAGAAPVAREMFHAAFPPGTFKKSQDITPSSPSTGQEGTFEAR
CCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]