Click here to switch to the map view.

The map label for this gene is cpsA1 [H]

Identifier: 116327813

GI number: 116327813

Start: 1268571

End: 1269761

Strand: Direct

Name: cpsA1 [H]

Synonym: LBL_1080

Alternate gene names: 116327813

Gene position: 1268571-1269761 (Clockwise)

Preceding gene: 116327812

Following gene: 116327816

Centisome position: 35.1

GC content: 46.1

Gene sequence:

>1191_bases
ATGGTTCGAATGATACCTACAGTAACCACAGATCGAATCGAAGAACTCATACGTTATAGAAGGCAAATTCATAAACATCC
GGAACTGAGATACGAGGAAAATCAAACCGCAGGTTATGTGATCGATCATTTGAAAAGTTTAGGTTTCCCTTTTCAGGATA
AAATCGCGAAAACCGGAGTCGTATCCTTGATCGATTCCGGCAAACCCGGAAAAACGCTTCTCGTACGGGCGGATATGGAC
GCCTTACCTATCTTAGAGGAATCTCGCAAGGAATACAAATCTGTTCACGATGGGATCATGCACGCCTGCGGTCACGATGC
TCATACTTCCATTCTCATGGGACTTGCGACGGAAATCAAAGAGGACATCAGGTCCGTTATTCCCAAAGGAAAGGTTTTAT
TGGTATTCCAGCCTGCGGAAGAAGGCGGGCAGGGAGCAGATAGGATGATCGAAGAAGGCATATTAGAGAAGTATAACATA
GATGCCGCCTTGGCTTTGCATGTGTGGAACCATATTCCCGTAGGAAAAGTAGGAGTCGTAGATGGACCGATGATGGCCGC
CGTGGATGAATTCACCATCGTCGTCTCCGGAATCAGCGGTCATGGGGCCATGCCTCAGCACACTGTGGATCCGATCGTAG
TCGGGGCCCAGATCGTAAACGCGTTGCAGACTATCGTTTCTAGAAATACGGATCCTCTTGATTCTTGTGTGGTAACTGTC
GGGAGTTTTCATGCAGGAAACGCGTTTAACGTGATCCCGGAAACCGCGGAATTAAAAGGAACCGTGAGAACGTATTCCAA
AAGGATGTTCGAGGAAGTTCCTGAAAAATTGGAAAGAGTGGTTAGTGGAATTGCGTCTGCGCTTGGTGCGAAAGTTTCCA
TCCGCTACGAAAGAACCAATCAGCCCACGATCAACGATTCGGAAATAGCAAACATAGTCCGTAAAGCTTCGTTGAACGTT
CTTGGCCCGGGAAATGTAACGGAAGAAAACACGAAATCTATGGGAGGAGAGGATTTCTCCGCATTTCTTATGAAGGTTCC
GGGTTGTTATTTTTTCGTAGGTTCTAGAAATGAAGAGAAAGGATTCGTTTATCCGCATCATAGTTCCAAGTTCGACATAG
ACGAGGATTCTCTGTCGATCGGTCTTAGCGTGCTCAAAGAGGCAATTAAAATCTATCACGAAGAAAACTGA

Upstream 100 bases:

>100_bases
ACAAAAGATCTTTTTTGCAAAGTTAAGGATATACTTTTGTAATTTAAGAGTTTTTAAGCGAAATCTAAAATTCTTTCCTA
AAACGGTTTAGGATAGTAAA

Downstream 100 bases:

>100_bases
TTGAGATAACGTGAATTCGGCATAACAAATGTGAAAGCGATTATTATGCTGCGATCCATAGCGAGCCCATTTTGGTTTTA
GTACAGTCCGGCCTTTATTT

Product: metal- dependentamidase/aminoacylase/carboxypeptidase

Products: carboxylate; L-amino acid

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MVRMIPTVTTDRIEELIRYRRQIHKHPELRYEENQTAGYVIDHLKSLGFPFQDKIAKTGVVSLIDSGKPGKTLLVRADMD
ALPILEESRKEYKSVHDGIMHACGHDAHTSILMGLATEIKEDIRSVIPKGKVLLVFQPAEEGGQGADRMIEEGILEKYNI
DAALALHVWNHIPVGKVGVVDGPMMAAVDEFTIVVSGISGHGAMPQHTVDPIVVGAQIVNALQTIVSRNTDPLDSCVVTV
GSFHAGNAFNVIPETAELKGTVRTYSKRMFEEVPEKLERVVSGIASALGAKVSIRYERTNQPTINDSEIANIVRKASLNV
LGPGNVTEENTKSMGGEDFSAFLMKVPGCYFFVGSRNEEKGFVYPHHSSKFDIDEDSLSIGLSVLKEAIKIYHEEN

Sequences:

>Translated_396_residues
MVRMIPTVTTDRIEELIRYRRQIHKHPELRYEENQTAGYVIDHLKSLGFPFQDKIAKTGVVSLIDSGKPGKTLLVRADMD
ALPILEESRKEYKSVHDGIMHACGHDAHTSILMGLATEIKEDIRSVIPKGKVLLVFQPAEEGGQGADRMIEEGILEKYNI
DAALALHVWNHIPVGKVGVVDGPMMAAVDEFTIVVSGISGHGAMPQHTVDPIVVGAQIVNALQTIVSRNTDPLDSCVVTV
GSFHAGNAFNVIPETAELKGTVRTYSKRMFEEVPEKLERVVSGIASALGAKVSIRYERTNQPTINDSEIANIVRKASLNV
LGPGNVTEENTKSMGGEDFSAFLMKVPGCYFFVGSRNEEKGFVYPHHSSKFDIDEDSLSIGLSVLKEAIKIYHEEN
>Mature_396_residues
MVRMIPTVTTDRIEELIRYRRQIHKHPELRYEENQTAGYVIDHLKSLGFPFQDKIAKTGVVSLIDSGKPGKTLLVRADMD
ALPILEESRKEYKSVHDGIMHACGHDAHTSILMGLATEIKEDIRSVIPKGKVLLVFQPAEEGGQGADRMIEEGILEKYNI
DAALALHVWNHIPVGKVGVVDGPMMAAVDEFTIVVSGISGHGAMPQHTVDPIVVGAQIVNALQTIVSRNTDPLDSCVVTV
GSFHAGNAFNVIPETAELKGTVRTYSKRMFEEVPEKLERVVSGIASALGAKVSIRYERTNQPTINDSEIANIVRKASLNV
LGPGNVTEENTKSMGGEDFSAFLMKVPGCYFFVGSRNEEKGFVYPHHSSKFDIDEDSLSIGLSVLKEAIKIYHEEN

Specific function: Can release basic, acidic, aromatic, and, to a lesser extent, aliphatic amino acids [H]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=423, Percent_Identity=27.1867612293144, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1787598, Length=306, Percent_Identity=23.8562091503268, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: 3.5.1.14

Molecular weight: Translated: 43499; Mature: 43499

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRMIPTVTTDRIEELIRYRRQIHKHPELRYEENQTAGYVIDHLKSLGFPFQDKIAKTGV
CCEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHH
VSLIDSGKPGKTLLVRADMDALPILEESRKEYKSVHDGIMHACGHDAHTSILMGLATEIK
HHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
EDIRSVIPKGKVLLVFQPAEEGGQGADRMIEEGILEKYNIDAALALHVWNHIPVGKVGVV
HHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHEEEHHHCCCCCCCCCCC
DGPMMAAVDEFTIVVSGISGHGAMPQHTVDPIVVGAQIVNALQTIVSRNTDPLDSCVVTV
CCCHHHHHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
GSFHAGNAFNVIPETAELKGTVRTYSKRMFEEVPEKLERVVSGIASALGAKVSIRYERTN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCC
QPTINDSEIANIVRKASLNVLGPGNVTEENTKSMGGEDFSAFLMKVPGCYFFVGSRNEEK
CCCCCHHHHHHHHHHHCCCEECCCCCCCCHHHHCCCCHHHHHHHHCCCEEEEEECCCCCC
GFVYPHHSSKFDIDEDSLSIGLSVLKEAIKIYHEEN
CEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVRMIPTVTTDRIEELIRYRRQIHKHPELRYEENQTAGYVIDHLKSLGFPFQDKIAKTGV
CCEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHH
VSLIDSGKPGKTLLVRADMDALPILEESRKEYKSVHDGIMHACGHDAHTSILMGLATEIK
HHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
EDIRSVIPKGKVLLVFQPAEEGGQGADRMIEEGILEKYNIDAALALHVWNHIPVGKVGVV
HHHHHHCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCHHEEEHHHCCCCCCCCCCC
DGPMMAAVDEFTIVVSGISGHGAMPQHTVDPIVVGAQIVNALQTIVSRNTDPLDSCVVTV
CCCHHHHHHHEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
GSFHAGNAFNVIPETAELKGTVRTYSKRMFEEVPEKLERVVSGIASALGAKVSIRYERTN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCC
QPTINDSEIANIVRKASLNVLGPGNVTEENTKSMGGEDFSAFLMKVPGCYFFVGSRNEEK
CCCCCHHHHHHHHHHHCCCEECCCCCCCCHHHHCCCCHHHHHHHHCCCEEEEEECCCCCC
GFVYPHHSSKFDIDEDSLSIGLSVLKEAIKIYHEEN
CEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: N-acyl-L-amino acid; H2O

Specific reaction: An N-acyl-L-amino acid + H2O = a carboxylate + an L-amino acid

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7559343; 11427726; 1597179 [H]