Click here to switch to the map view.

The map label for this gene is trpE [H]

Identifier: 116327039

GI number: 116327039

Start: 254288

End: 255709

Strand: Direct

Name: trpE [H]

Synonym: LBL_0212

Alternate gene names: 116327039

Gene position: 254288-255709 (Clockwise)

Preceding gene: 116327038

Following gene: 116327040

Centisome position: 7.04

GC content: 43.11

Gene sequence:

>1422_bases
ATGGAAACTCCGGTTTCCCTGTTTGCGAAGTGGGGATGTGCGTCTTCAGAGAATTCCTTTCTTTTGGAATCAGTGGAAGG
CCGTGAAAAGCTGGGAAGAAATTCTTTTCTCGGCAAAACTCCTTCTCGTATCCTTCAAGGAAAGAATGGTCTTTTTTATA
TCACCGTCGAAAACGAACCTTCTACGGAAATCATCACTTACGATCCTTTGGTTCTCTTGGAAAATCTTCTCGGAGACGAC
GTTTACGTCCAAGACTATCGTCTTCCTTCGTTTGCCGGTGGGGCGGTAGGATTCGTGTCTTTTGCGGCGGTTCGCTATTA
TGAAAACATTCCTGATACGAAACCGGAAGATGAAAACGCCCCCGATTGTTATTTTGCGATCTACGACGAACTTCTTGTGG
TCGATCATATCGATCATCTTTTAAGAATCGTAGTTAACGCAAGAATCGGAGAACATTCTTCTTTGAAAGAATGTTATGAT
TCCACGATCAATAAGATCGATTCTATCGAAAATGAAATCCGAAACGGAATTGTTCCGGAAGAGATCAAAAACCCGAAGGT
TGTAAGCGGGGTTATGCAATTGAATCCGAATATTCCGGATGAAGAATATAAAAAAAATGTGGAAAAGGCCAAGAAATACA
TCCAAGCCGGGGATATCTTTCAAGTCGTGCCTTCCAGAAAGTTGAAGTTCAAACCGGATATTTCTCCGTTTCAAATCTAT
CGCGGCCTAAGAACAGTAAATCCGAGTCCTTATATGTATTATCTCCGACTCGGCGAAATTACGATTGTAGGAAGTTCTCC
GGAGATTATGGTGAAATATGCGAACAATCAGACGTTTCTTCGTCCGATTGCAGGAACCCGTCCTCGCGGTAAAAATTCGA
ACGAAGATAAATTTTTGGAAGAAAATCTTCTCTCCGATCCGAAGGAAATATCGGAGCATGTTATGCTTGTCGATCTCGGA
CGCAACGATCTCGGAAAGGTTTGTAAACCCGGAAGCGTTCGTGTGAATGATTTTAAAGTGATTGAGAAATATTCTCACGT
TATGCACATTGTAAGTCAATGTGCAGGGGAACTCGATGAAAGTAAGTCCGTCTATGATTTGATCCGTGCGACTCTTCCTG
CAGGAACTGTTTCAGGCGCTCCGAAAATCCGTGCGATGGAAATCATAGACGAACTGGAAGCGACTCGAAGAGCGATTTAT
TCCGGAGCTTTGGGTTATATTTCATTTTCCGGAGAAAGTGATCTTGCAATTATCATCCGGACGATCGTGTTTTACGGAGA
CACTGCATTCTTACAGGCGGGTGGTGGAGTTGTATATGATTCGGTTGCCGAGTTGGAACTGGAAGAAACAAAAAACAAAA
TGGCGGCTCTTTTAAAGGCGGTAGAGTTTGCAAGAAACGGGCTCAAAGGGGAATGGAAATAA

Upstream 100 bases:

>100_bases
GACCGAACTCCTGAAAGAGTTTTCTCTCACATTCGAGGAGTTCTCCGCGCTTGCAGAACGCGGGAATTTAATTCCTGTTT
TTAAACAGGTTTTTCTAGAT

Downstream 100 bases:

>100_bases
TGCTTCTCCTAATTGATAATTACGATTCTTTCACGTACAATCTTTATCAATATTTTTGTCAGATCGGAAACGAAGTCGAA
GTTTATCGAAACGATAAAAT

Product: anthranilate synthase (component I)

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
METPVSLFAKWGCASSENSFLLESVEGREKLGRNSFLGKTPSRILQGKNGLFYITVENEPSTEIITYDPLVLLENLLGDD
VYVQDYRLPSFAGGAVGFVSFAAVRYYENIPDTKPEDENAPDCYFAIYDELLVVDHIDHLLRIVVNARIGEHSSLKECYD
STINKIDSIENEIRNGIVPEEIKNPKVVSGVMQLNPNIPDEEYKKNVEKAKKYIQAGDIFQVVPSRKLKFKPDISPFQIY
RGLRTVNPSPYMYYLRLGEITIVGSSPEIMVKYANNQTFLRPIAGTRPRGKNSNEDKFLEENLLSDPKEISEHVMLVDLG
RNDLGKVCKPGSVRVNDFKVIEKYSHVMHIVSQCAGELDESKSVYDLIRATLPAGTVSGAPKIRAMEIIDELEATRRAIY
SGALGYISFSGESDLAIIIRTIVFYGDTAFLQAGGGVVYDSVAELELEETKNKMAALLKAVEFARNGLKGEWK

Sequences:

>Translated_473_residues
METPVSLFAKWGCASSENSFLLESVEGREKLGRNSFLGKTPSRILQGKNGLFYITVENEPSTEIITYDPLVLLENLLGDD
VYVQDYRLPSFAGGAVGFVSFAAVRYYENIPDTKPEDENAPDCYFAIYDELLVVDHIDHLLRIVVNARIGEHSSLKECYD
STINKIDSIENEIRNGIVPEEIKNPKVVSGVMQLNPNIPDEEYKKNVEKAKKYIQAGDIFQVVPSRKLKFKPDISPFQIY
RGLRTVNPSPYMYYLRLGEITIVGSSPEIMVKYANNQTFLRPIAGTRPRGKNSNEDKFLEENLLSDPKEISEHVMLVDLG
RNDLGKVCKPGSVRVNDFKVIEKYSHVMHIVSQCAGELDESKSVYDLIRATLPAGTVSGAPKIRAMEIIDELEATRRAIY
SGALGYISFSGESDLAIIIRTIVFYGDTAFLQAGGGVVYDSVAELELEETKNKMAALLKAVEFARNGLKGEWK
>Mature_473_residues
METPVSLFAKWGCASSENSFLLESVEGREKLGRNSFLGKTPSRILQGKNGLFYITVENEPSTEIITYDPLVLLENLLGDD
VYVQDYRLPSFAGGAVGFVSFAAVRYYENIPDTKPEDENAPDCYFAIYDELLVVDHIDHLLRIVVNARIGEHSSLKECYD
STINKIDSIENEIRNGIVPEEIKNPKVVSGVMQLNPNIPDEEYKKNVEKAKKYIQAGDIFQVVPSRKLKFKPDISPFQIY
RGLRTVNPSPYMYYLRLGEITIVGSSPEIMVKYANNQTFLRPIAGTRPRGKNSNEDKFLEENLLSDPKEISEHVMLVDLG
RNDLGKVCKPGSVRVNDFKVIEKYSHVMHIVSQCAGELDESKSVYDLIRATLPAGTVSGAPKIRAMEIIDELEATRRAIY
SGALGYISFSGESDLAIIIRTIVFYGDTAFLQAGGGVVYDSVAELELEETKNKMAALLKAVEFARNGLKGEWK

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=409, Percent_Identity=38.3863080684597, Blast_Score=264, Evalue=1e-71,
Organism=Escherichia coli, GI1788114, Length=415, Percent_Identity=33.2530120481928, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI87082077, Length=272, Percent_Identity=25, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1786809, Length=265, Percent_Identity=22.6415094339623, Blast_Score=65, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6320935, Length=455, Percent_Identity=38.4615384615385, Blast_Score=303, Evalue=4e-83,
Organism=Saccharomyces cerevisiae, GI6324361, Length=411, Percent_Identity=26.2773722627737, Blast_Score=132, Evalue=1e-31,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 52851; Mature: 52851

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METPVSLFAKWGCASSENSFLLESVEGREKLGRNSFLGKTPSRILQGKNGLFYITVENEP
CCCHHHHHHHCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCHHHHHCCCCCEEEEEECCCC
STEIITYDPLVLLENLLGDDVYVQDYRLPSFAGGAVGFVSFAAVRYYENIPDTKPEDENA
CCEEEEECHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
PDCYFAIYDELLVVDHIDHLLRIVVNARIGEHSSLKECYDSTINKIDSIENEIRNGIVPE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCH
EIKNPKVVSGVMQLNPNIPDEEYKKNVEKAKKYIQAGDIFQVVPSRKLKFKPDISPFQIY
HHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEECCCCCHHHHH
RGLRTVNPSPYMYYLRLGEITIVGSSPEIMVKYANNQTFLRPIAGTRPRGKNSNEDKFLE
HHHHCCCCCCEEEEEEECCEEEECCCCCEEEEECCCCEEEEECCCCCCCCCCCCHHHHHH
ENLLSDPKEISEHVMLVDLGRNDLGKVCKPGSVRVNDFKVIEKYSHVMHIVSQCAGELDE
HHHCCCHHHHHCCEEEEECCCCCHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCC
SKSVYDLIRATLPAGTVSGAPKIRAMEIIDELEATRRAIYSGALGYISFSGESDLAIIIR
CHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHH
TIVFYGDTAFLQAGGGVVYDSVAELELEETKNKMAALLKAVEFARNGLKGEWK
HHHHHCCHHEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
METPVSLFAKWGCASSENSFLLESVEGREKLGRNSFLGKTPSRILQGKNGLFYITVENEP
CCCHHHHHHHCCCCCCCCCEEHHHHHHHHHHCCCCCCCCCHHHHHCCCCCEEEEEECCCC
STEIITYDPLVLLENLLGDDVYVQDYRLPSFAGGAVGFVSFAAVRYYENIPDTKPEDENA
CCEEEEECHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
PDCYFAIYDELLVVDHIDHLLRIVVNARIGEHSSLKECYDSTINKIDSIENEIRNGIVPE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCH
EIKNPKVVSGVMQLNPNIPDEEYKKNVEKAKKYIQAGDIFQVVPSRKLKFKPDISPFQIY
HHCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEECCCCCHHHHH
RGLRTVNPSPYMYYLRLGEITIVGSSPEIMVKYANNQTFLRPIAGTRPRGKNSNEDKFLE
HHHHCCCCCCEEEEEEECCEEEECCCCCEEEEECCCCEEEEECCCCCCCCCCCCHHHHHH
ENLLSDPKEISEHVMLVDLGRNDLGKVCKPGSVRVNDFKVIEKYSHVMHIVSQCAGELDE
HHHCCCHHHHHCCEEEEECCCCCHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCC
SKSVYDLIRATLPAGTVSGAPKIRAMEIIDELEATRRAIYSGALGYISFSGESDLAIIIR
CHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHH
TIVFYGDTAFLQAGGGVVYDSVAELELEETKNKMAALLKAVEFARNGLKGEWK
HHHHHCCHHEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2732211 [H]