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The map label for this gene is ada [H]

Identifier: 116326974

GI number: 116326974

Start: 164018

End: 164866

Strand: Direct

Name: ada [H]

Synonym: LBL_0141

Alternate gene names: 116326974

Gene position: 164018-164866 (Clockwise)

Preceding gene: 116326973

Following gene: 116326975

Centisome position: 4.54

GC content: 39.58

Gene sequence:

>849_bases
ATGGAACATTATAAAAAAATCGCAGAAGCAATACAGTTCATACAAAAACACGCCGTTTCTCAGCCTGAATTAGATGAAAT
TGCAAAGTCAGTGGGCTTGAGTCCGTTTCATTTTCAAAGACTTTTTACCGAATGGGCCGGCGTCAGTCCGAAACAATTTC
TGCAATACCTTACGTTGCAGAATGCAAAATCCATACTTTCTAAACCCCAAACGACTCTGTTTGATGCCGCATTTGAAGTT
GGTTTGTCCGGAACAAGTCGATTGCACGATTTATTCGTGAAAATCGAAGGAATGACTCCCGGAGAATTTAAAAACGGCGG
AGAAAAACTCAAGATCCGATACAGTTTTCAGAAAAATGTTTTTGGTGATTACGTAATCGCCTCCACGGAAAAAGGGATTT
GTAATTTATACTTTTATGATGTTTCTAAAGAACAGGTGGTTTCCGAATTGAAAGAACAATGGAGTAAGGCAAATCTGATT
CAGAAAACGGATGAGAATCAAAATCGAGTCATTCGTTTTTTTGATAAAACATTCGACGGAAAAGAAAAAATCAAACTTCA
TCTTAAAGGAACGGATTTTCAAATCAAGGTTTGGGAAGCCCTTCTTAAAATTCCGGAGGGCCAATTATCTTCTTACATGG
ATATTGCGAATTCCATTGGGCAGGAAAATGCTTCGAGGGCTGTTGGGACTGCGATCGGAAAAAATCCAATCGGATATCTG
ATTCCTTGTCATCGTGTGATCAAAAGTACAGGTAGCATTGGAGAATATCGCTGGGGCCCCGAGAGAAAAATGGCCATGAT
AGGTTGGGAAATGAGCAGAACAGATCATTTAGTTTCGAGCATATCCTAA

Upstream 100 bases:

>100_bases
ACAAAGACAAAGTTAAAAAACAAATTGGGAAATTCCAAAATATCCTAAAAGTAGCAAGGATCGGATTTTAAAAATTTGAT
TTTCGGATAACATTCTAAAT

Downstream 100 bases:

>100_bases
AATGGTGCAAAACTTGATTATAAGCATTGTTTCGATTCCTTTGCTTTTCTGAAAGTTTTTTCTTCCAATTATTTTCTGGC
TGAAATAACCTAAAATTCTA

Product: methylated-DNA--[protein]-cysteine S- methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MEHYKKIAEAIQFIQKHAVSQPELDEIAKSVGLSPFHFQRLFTEWAGVSPKQFLQYLTLQNAKSILSKPQTTLFDAAFEV
GLSGTSRLHDLFVKIEGMTPGEFKNGGEKLKIRYSFQKNVFGDYVIASTEKGICNLYFYDVSKEQVVSELKEQWSKANLI
QKTDENQNRVIRFFDKTFDGKEKIKLHLKGTDFQIKVWEALLKIPEGQLSSYMDIANSIGQENASRAVGTAIGKNPIGYL
IPCHRVIKSTGSIGEYRWGPERKMAMIGWEMSRTDHLVSSIS

Sequences:

>Translated_282_residues
MEHYKKIAEAIQFIQKHAVSQPELDEIAKSVGLSPFHFQRLFTEWAGVSPKQFLQYLTLQNAKSILSKPQTTLFDAAFEV
GLSGTSRLHDLFVKIEGMTPGEFKNGGEKLKIRYSFQKNVFGDYVIASTEKGICNLYFYDVSKEQVVSELKEQWSKANLI
QKTDENQNRVIRFFDKTFDGKEKIKLHLKGTDFQIKVWEALLKIPEGQLSSYMDIANSIGQENASRAVGTAIGKNPIGYL
IPCHRVIKSTGSIGEYRWGPERKMAMIGWEMSRTDHLVSSIS
>Mature_282_residues
MEHYKKIAEAIQFIQKHAVSQPELDEIAKSVGLSPFHFQRLFTEWAGVSPKQFLQYLTLQNAKSILSKPQTTLFDAAFEV
GLSGTSRLHDLFVKIEGMTPGEFKNGGEKLKIRYSFQKNVFGDYVIASTEKGICNLYFYDVSKEQVVSELKEQWSKANLI
QKTDENQNRVIRFFDKTFDGKEKIKLHLKGTDFQIKVWEALLKIPEGQLSSYMDIANSIGQENASRAVGTAIGKNPIGYL
IPCHRVIKSTGSIGEYRWGPERKMAMIGWEMSRTDHLVSSIS

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788542, Length=268, Percent_Identity=30.9701492537313, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1787596, Length=74, Percent_Identity=47.2972972972973, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI115533068, Length=123, Percent_Identity=36.5853658536585, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI115533070, Length=123, Percent_Identity=36.5853658536585, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6320001, Length=83, Percent_Identity=42.1686746987952, Blast_Score=80, Evalue=4e-16,
Organism=Drosophila melanogaster, GI17137554, Length=172, Percent_Identity=30.2325581395349, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 32023; Mature: 32023

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHYKKIAEAIQFIQKHAVSQPELDEIAKSVGLSPFHFQRLFTEWAGVSPKQFLQYLTLQ
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
NAKSILSKPQTTLFDAAFEVGLSGTSRLHDLFVKIEGMTPGEFKNGGEKLKIRYSFQKNV
HHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHEEECCCCCCCCCCCCCEEEEEEEECCCC
FGDYVIASTEKGICNLYFYDVSKEQVVSELKEQWSKANLIQKTDENQNRVIRFFDKTFDG
CCCEEEEECCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHCCCC
KEKIKLHLKGTDFQIKVWEALLKIPEGQLSSYMDIANSIGQENASRAVGTAIGKNPIGYL
CEEEEEEECCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHH
IPCHRVIKSTGSIGEYRWGPERKMAMIGWEMSRTDHLVSSIS
HHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCC
>Mature Secondary Structure
MEHYKKIAEAIQFIQKHAVSQPELDEIAKSVGLSPFHFQRLFTEWAGVSPKQFLQYLTLQ
CHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHH
NAKSILSKPQTTLFDAAFEVGLSGTSRLHDLFVKIEGMTPGEFKNGGEKLKIRYSFQKNV
HHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHEEECCCCCCCCCCCCCEEEEEEEECCCC
FGDYVIASTEKGICNLYFYDVSKEQVVSELKEQWSKANLIQKTDENQNRVIRFFDKTFDG
CCCEEEEECCCCEEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHCCCC
KEKIKLHLKGTDFQIKVWEALLKIPEGQLSSYMDIANSIGQENASRAVGTAIGKNPIGYL
CEEEEEEECCCCEEHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHH
IPCHRVIKSTGSIGEYRWGPERKMAMIGWEMSRTDHLVSSIS
HHHHHHHHCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]