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The map label for this gene is dgoD [H]

Identifier: 116249447

GI number: 116249447

Start: 857629

End: 858816

Strand: Direct

Name: dgoD [H]

Synonym: pRL120785

Alternate gene names: 116249447

Gene position: 857629-858816 (Clockwise)

Preceding gene: 116249446

Following gene: 116249455

Centisome position: 98.58

GC content: 62.29

Gene sequence:

>1188_bases
ATGAAGATCGACCGCATGCGGGTTTTCATGACCCGCGACAAGGACCGTCCGCGCGTGATCGTCGCGCTCGACACCGATGA
CGGGCTGACGGGTTGGGGCGAATGCTACAATCACGGCCCCGACAAGGCGCTGCCGCCGCTGCTCGATTATCTCTACGGAT
TTGTCTCCGGCCAGGATCCGACACGCGTGGAGTATCTCGTCAATCTGCTGATCCAGCAGAGCCGGTTTCCGCCGGGGGCG
CTTGGCCTTGCCGCGATCTCCGCGCTCGATCACTGCCTGTGGGACCTTGCGGCCAAGGCAGTGAATGTCCCGGTCTACAA
GCTGCTCGGCGGCGAAGTGCGCGACCGCATCAAGGTCTACGCCGGTGTCTATACCGCACCGGATGCGCCGGCGGCGCGCG
ACGAGTTCGATCGTCTGAAGGAGGGATGGGGGTTCACCGCCTTCAAGCTCAGCCCCTGGCGGATCGACATGCACTCCAAC
CGCTGGGGTAATGTCGTGAAAGCCTCGGCGGATTATTTCCGCTCGCTTCGCGAAACGGTCAATGACGAATACGAAATCGC
CTTCGATGCCCATGCGAAGATTTTCGAGCCGATCGCCGCTCGCCAGCTCGGCAATGCGCTGGCGCCTTATGATCCGCTGT
TTTTCGAGGAGCCGCTGCGTCCTGAAAATATCGAGGCCTGGGGCGACCTGAAACAGGGGCTCAACTGCACACTTGCGACC
GGCGAGTCGCTCTATAACAGAAACGAGTTCCTGCGGCTGCTGCAGGTCAAGGGCGCCGACCTCATCCAGCCCGACATCTG
CGTCGTCGGCGGCATCAGTGAAATGCGCCGCATCGCCACACTCGCCGAAGCCTTCTTCGTCGGCGTGGCGCCGCACAACC
CGATGGGTCCGCTTGCGACGGCGGTCAACGTTCATTTTTCTGCGGCGGCGCAGAATTTCCGCATCCTCGAATACCGGCTG
CCAAAGGGACAGGCTTATGTCTATGGCGGCCTCGATATCGAGAAGCGGGAGGGGGAAACCCGCTACGTCGTCGATCCCTA
TCTGCCGAAGGACGGCTATCTGGAGCTGCGTCCCGACCGGCCGGGCTGGGGCGTCGAAATGGACGAGAAGGCGATGGAAG
AGGAAGGTTACATCCATTGGCAGCGACGCGTGCCGAAGCGGCCCGACGGCTCCTACGCCTTTGCCTGA

Upstream 100 bases:

>100_bases
AAGTGCCGGTGCCTACAATCCCGGAACCGACAATGAGCATCTCTACGTGCAGACGCGCCAGTCGCGTCGCACCTATTTCA
ATCGCTAGAAGGACATAAAG

Downstream 100 bases:

>100_bases
GGGCATTGCCCGCTTAAAACAGGAGCTGGCCGCCCGCATGGCGCGGCCAGCTCCCGCAGGCGACGCTTCTAGAGCATGAA
GCCGAAAAGTGTGAGCGGTT

Product: putative racemase

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MKIDRMRVFMTRDKDRPRVIVALDTDDGLTGWGECYNHGPDKALPPLLDYLYGFVSGQDPTRVEYLVNLLIQQSRFPPGA
LGLAAISALDHCLWDLAAKAVNVPVYKLLGGEVRDRIKVYAGVYTAPDAPAARDEFDRLKEGWGFTAFKLSPWRIDMHSN
RWGNVVKASADYFRSLRETVNDEYEIAFDAHAKIFEPIAARQLGNALAPYDPLFFEEPLRPENIEAWGDLKQGLNCTLAT
GESLYNRNEFLRLLQVKGADLIQPDICVVGGISEMRRIATLAEAFFVGVAPHNPMGPLATAVNVHFSAAAQNFRILEYRL
PKGQAYVYGGLDIEKREGETRYVVDPYLPKDGYLELRPDRPGWGVEMDEKAMEEEGYIHWQRRVPKRPDGSYAFA

Sequences:

>Translated_395_residues
MKIDRMRVFMTRDKDRPRVIVALDTDDGLTGWGECYNHGPDKALPPLLDYLYGFVSGQDPTRVEYLVNLLIQQSRFPPGA
LGLAAISALDHCLWDLAAKAVNVPVYKLLGGEVRDRIKVYAGVYTAPDAPAARDEFDRLKEGWGFTAFKLSPWRIDMHSN
RWGNVVKASADYFRSLRETVNDEYEIAFDAHAKIFEPIAARQLGNALAPYDPLFFEEPLRPENIEAWGDLKQGLNCTLAT
GESLYNRNEFLRLLQVKGADLIQPDICVVGGISEMRRIATLAEAFFVGVAPHNPMGPLATAVNVHFSAAAQNFRILEYRL
PKGQAYVYGGLDIEKREGETRYVVDPYLPKDGYLELRPDRPGWGVEMDEKAMEEEGYIHWQRRVPKRPDGSYAFA
>Mature_395_residues
MKIDRMRVFMTRDKDRPRVIVALDTDDGLTGWGECYNHGPDKALPPLLDYLYGFVSGQDPTRVEYLVNLLIQQSRFPPGA
LGLAAISALDHCLWDLAAKAVNVPVYKLLGGEVRDRIKVYAGVYTAPDAPAARDEFDRLKEGWGFTAFKLSPWRIDMHSN
RWGNVVKASADYFRSLRETVNDEYEIAFDAHAKIFEPIAARQLGNALAPYDPLFFEEPLRPENIEAWGDLKQGLNCTLAT
GESLYNRNEFLRLLQVKGADLIQPDICVVGGISEMRRIATLAEAFFVGVAPHNPMGPLATAVNVHFSAAAQNFRILEYRL
PKGQAYVYGGLDIEKREGETRYVVDPYLPKDGYLELRPDRPGWGVEMDEKAMEEEGYIHWQRRVPKRPDGSYAFA

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=264, Percent_Identity=25, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI186972148, Length=357, Percent_Identity=24.0896358543417, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI48994953, Length=379, Percent_Identity=32.4538258575198, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1787864, Length=398, Percent_Identity=27.3869346733668, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI226510960, Length=371, Percent_Identity=25.3369272237197, Blast_Score=96, Evalue=6e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 44575; Mature: 44575

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDRMRVFMTRDKDRPRVIVALDTDDGLTGWGECYNHGPDKALPPLLDYLYGFVSGQDP
CCCCCEEEEEECCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCC
TRVEYLVNLLIQQSRFPPGALGLAAISALDHCLWDLAAKAVNVPVYKLLGGEVRDRIKVY
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHEEE
AGVYTAPDAPAARDEFDRLKEGWGFTAFKLSPWRIDMHSNRWGNVVKASADYFRSLRETV
EEEEECCCCCCHHHHHHHHHHCCCEEEEEECCEEEEECCCCCCCEEEHHHHHHHHHHHHC
NDEYEIAFDAHAKIFEPIAARQLGNALAPYDPLFFEEPLRPENIEAWGDLKQGLNCTLAT
CCCEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCEEEEEC
GESLYNRNEFLRLLQVKGADLIQPDICVVGGISEMRRIATLAEAFFVGVAPHNPMGPLAT
CHHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHEECCCCCCCCCHHH
AVNVHFSAAAQNFRILEYRLPKGQAYVYGGLDIEKREGETRYVVDPYLPKDGYLELRPDR
HEEEEEHHHCCCEEEEEEECCCCCEEEEECCEEECCCCCEEEEECCCCCCCCCEEECCCC
PGWGVEMDEKAMEEEGYIHWQRRVPKRPDGSYAFA
CCCCCCCCHHHHHHCCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MKIDRMRVFMTRDKDRPRVIVALDTDDGLTGWGECYNHGPDKALPPLLDYLYGFVSGQDP
CCCCCEEEEEECCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCC
TRVEYLVNLLIQQSRFPPGALGLAAISALDHCLWDLAAKAVNVPVYKLLGGEVRDRIKVY
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCHHHHHHHEEE
AGVYTAPDAPAARDEFDRLKEGWGFTAFKLSPWRIDMHSNRWGNVVKASADYFRSLRETV
EEEEECCCCCCHHHHHHHHHHCCCEEEEEECCEEEEECCCCCCCEEEHHHHHHHHHHHHC
NDEYEIAFDAHAKIFEPIAARQLGNALAPYDPLFFEEPLRPENIEAWGDLKQGLNCTLAT
CCCEEEEEECHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCEEEEEC
GESLYNRNEFLRLLQVKGADLIQPDICVVGGISEMRRIATLAEAFFVGVAPHNPMGPLAT
CHHHHHHHHHHHHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHHEECCCCCCCCCHHH
AVNVHFSAAAQNFRILEYRLPKGQAYVYGGLDIEKREGETRYVVDPYLPKDGYLELRPDR
HEEEEEHHHCCCEEEEEEECCCCCEEEEECCEEECCCCCEEEEECCCCCCCCCEEECCCC
PGWGVEMDEKAMEEEGYIHWQRRVPKRPDGSYAFA
CCCCCCCCHHHHHHCCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]