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The map label for this gene is dgoD1 [H]

Identifier: 116249446

GI number: 116249446

Start: 856405

End: 857616

Strand: Direct

Name: dgoD1 [H]

Synonym: pRL120784

Alternate gene names: 116249446

Gene position: 856405-857616 (Clockwise)

Preceding gene: 116249445

Following gene: 116249447

Centisome position: 98.43

GC content: 63.7

Gene sequence:

>1212_bases
ATGAAAATCACCGGGATCCGCACTTTTCTCATGCATGTCGGTCAGCCCGATCCCGCGAACTGGGCTTCCGACCAACGTTC
TGGCGGTGGCGCCTCCAAGCAGTTCGGCGGCACCAGAAACTGGCTCTTTCTGAAGATCGATACCGACGAGGGCATCACCG
GCATCGGTGAATGCTCAGGCTGGCCGCGCGTCGTCGAGACGGCGATTCATGATCTCGCCCCACTCCTCATCGGCGAAGAT
CCGGCCCATACCGAACGGCTATGGCAGAAGATGCATATCGCGATGATGGGCCATGGCATGCTCGGAACGGTCGGAGGCGG
TGCGATGACCGGCATCGACATGGCGCTATGGGACATCAAGGGCAAGGCGCTCGGCGTGCCTGTCTGGATGCTGCTCGGCG
GCAAGGTGCGCGACCGGATCCCGATCTATTCGCATGCGAATACGCCTGAGCGCGCGCTTGCCATCAAAACGCGCGGCATA
AAGGCGATCAAATGCGGCGGCGTCGCCGATCCGGTTCGCAAGGTTGCTGCGCTGCGCGACGCCGTCGGCGACGACATGGA
CATCGCCATCGATCTGCATGGGCCGCCGTGGCTGACGCCTGCGGATGCCTGCCGGCTGGTGCGGACGCTCGAACCCTATG
AATTGATGTGGGTGGAAGATCCGATCGCGCCGGAGAATATCGACGGCTACCGGCGTATCCGCGACGCCGCGCATGTGCCG
CTTGCCGCCGGCGAGCGCTCGGCGACCATCTTCGGCGAGCGTGAGCTCATCGAGAAGGAGCTGGTCGACGTCATCCAGCC
GGACACCGGCCGGGCCGGCGGCATCACCCAGATGAAGAAGATCGCGGCCATGGCTGAAGCCCATCATATCCAGATGGCGC
CGCATTCGGGTTCGCTCGGGCCGGTGGCGGAATATGCGGCTTTGCATCTGCTTGCAGCGATCCCCAACGCCCTCATCCTC
GAGCGCCTCGACGACGACTGGGACGGCCGCCGCCAGACGATCGTGCCGCATCCGCAACAGGTCGACGGCTTGATCGCCGT
TCCTGATGGCCCGGGACTTGGCTGCGATATCGACGAGGCCTTCGTGGCGCGCTTCCCCAGCAACGGCAATGTCTCGGTGC
CGCAAAGTGCCGGTGCCTACAATCCCGGAACCGACAATGAGCATCTCTACGTGCAGACGCGCCAGTCGCGTCGCACCTAT
TTCAATCGCTAG

Upstream 100 bases:

>100_bases
TCATATCGGCGATTCCGCGCCCTGATCCGCGTGATCGCGATCAATCGCGGCGCTTCCGCTACGTCGCGCCGGACATTTTG
AAGAATGGGAGTTTTCAGCG

Downstream 100 bases:

>100_bases
AAGGACATAAAGATGAAGATCGACCGCATGCGGGTTTTCATGACCCGCGACAAGGACCGTCCGCGCGTGATCGTCGCGCT
CGACACCGATGACGGGCTGA

Product: putative racemase

Products: NA

Alternate protein names: GalD 1 [H]

Number of amino acids: Translated: 403; Mature: 403

Protein sequence:

>403_residues
MKITGIRTFLMHVGQPDPANWASDQRSGGGASKQFGGTRNWLFLKIDTDEGITGIGECSGWPRVVETAIHDLAPLLIGED
PAHTERLWQKMHIAMMGHGMLGTVGGGAMTGIDMALWDIKGKALGVPVWMLLGGKVRDRIPIYSHANTPERALAIKTRGI
KAIKCGGVADPVRKVAALRDAVGDDMDIAIDLHGPPWLTPADACRLVRTLEPYELMWVEDPIAPENIDGYRRIRDAAHVP
LAAGERSATIFGERELIEKELVDVIQPDTGRAGGITQMKKIAAMAEAHHIQMAPHSGSLGPVAEYAALHLLAAIPNALIL
ERLDDDWDGRRQTIVPHPQQVDGLIAVPDGPGLGCDIDEAFVARFPSNGNVSVPQSAGAYNPGTDNEHLYVQTRQSRRTY
FNR

Sequences:

>Translated_403_residues
MKITGIRTFLMHVGQPDPANWASDQRSGGGASKQFGGTRNWLFLKIDTDEGITGIGECSGWPRVVETAIHDLAPLLIGED
PAHTERLWQKMHIAMMGHGMLGTVGGGAMTGIDMALWDIKGKALGVPVWMLLGGKVRDRIPIYSHANTPERALAIKTRGI
KAIKCGGVADPVRKVAALRDAVGDDMDIAIDLHGPPWLTPADACRLVRTLEPYELMWVEDPIAPENIDGYRRIRDAAHVP
LAAGERSATIFGERELIEKELVDVIQPDTGRAGGITQMKKIAAMAEAHHIQMAPHSGSLGPVAEYAALHLLAAIPNALIL
ERLDDDWDGRRQTIVPHPQQVDGLIAVPDGPGLGCDIDEAFVARFPSNGNVSVPQSAGAYNPGTDNEHLYVQTRQSRRTY
FNR
>Mature_403_residues
MKITGIRTFLMHVGQPDPANWASDQRSGGGASKQFGGTRNWLFLKIDTDEGITGIGECSGWPRVVETAIHDLAPLLIGED
PAHTERLWQKMHIAMMGHGMLGTVGGGAMTGIDMALWDIKGKALGVPVWMLLGGKVRDRIPIYSHANTPERALAIKTRGI
KAIKCGGVADPVRKVAALRDAVGDDMDIAIDLHGPPWLTPADACRLVRTLEPYELMWVEDPIAPENIDGYRRIRDAAHVP
LAAGERSATIFGERELIEKELVDVIQPDTGRAGGITQMKKIAAMAEAHHIQMAPHSGSLGPVAEYAALHLLAAIPNALIL
ERLDDDWDGRRQTIVPHPQQVDGLIAVPDGPGLGCDIDEAFVARFPSNGNVSVPQSAGAYNPGTDNEHLYVQTRQSRRTY
FNR

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=354, Percent_Identity=37.0056497175141, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1787864, Length=377, Percent_Identity=32.6259946949602, Blast_Score=168, Evalue=6e-43,
Organism=Escherichia coli, GI226510960, Length=333, Percent_Identity=27.6276276276276, Blast_Score=111, Evalue=9e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 43725; Mature: 43725

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITGIRTFLMHVGQPDPANWASDQRSGGGASKQFGGTRNWLFLKIDTDEGITGIGECSG
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCC
WPRVVETAIHDLAPLLIGEDPAHTERLWQKMHIAMMGHGMLGTVGGGAMTGIDMALWDIK
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHEEEEEECC
GKALGVPVWMLLGGKVRDRIPIYSHANTPERALAIKTRGIKAIKCGGVADPVRKVAALRD
CCEECCEEEEEECCCHHCCCCEEECCCCCHHEEEEEECCEEEEECCCCHHHHHHHHHHHH
AVGDDMDIAIDLHGPPWLTPADACRLVRTLEPYELMWVEDPIAPENIDGYRRIRDAAHVP
HCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHCCC
LAAGERSATIFGERELIEKELVDVIQPDTGRAGGITQMKKIAAMAEAHHIQMAPHSGSLG
EECCCCCEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCCC
PVAEYAALHLLAAIPNALILERLDDDWDGRRQTIVPHPQQVDGLIAVPDGPGLGCDIDEA
HHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCHHCCCEEECCCCCCCCCCCCHH
FVARFPSNGNVSVPQSAGAYNPGTDNEHLYVQTRQSRRTYFNR
HHHCCCCCCCEECCCCCCCCCCCCCCCEEEEEECCHHHHCCCC
>Mature Secondary Structure
MKITGIRTFLMHVGQPDPANWASDQRSGGGASKQFGGTRNWLFLKIDTDEGITGIGECSG
CCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCC
WPRVVETAIHDLAPLLIGEDPAHTERLWQKMHIAMMGHGMLGTVGGGAMTGIDMALWDIK
CHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHEEEEEECC
GKALGVPVWMLLGGKVRDRIPIYSHANTPERALAIKTRGIKAIKCGGVADPVRKVAALRD
CCEECCEEEEEECCCHHCCCCEEECCCCCHHEEEEEECCEEEEECCCCHHHHHHHHHHHH
AVGDDMDIAIDLHGPPWLTPADACRLVRTLEPYELMWVEDPIAPENIDGYRRIRDAAHVP
HCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHHHCCC
LAAGERSATIFGERELIEKELVDVIQPDTGRAGGITQMKKIAAMAEAHHIQMAPHSGSLG
EECCCCCEEECCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCCCC
PVAEYAALHLLAAIPNALILERLDDDWDGRRQTIVPHPQQVDGLIAVPDGPGLGCDIDEA
HHHHHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCCCHHCCCEEECCCCCCCCCCCCHH
FVARFPSNGNVSVPQSAGAYNPGTDNEHLYVQTRQSRRTYFNR
HHHCCCCCCCEECCCCCCCCCCCCCCCEEEEEECCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA