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The map label for this gene is purR [H]

Identifier: 116249357

GI number: 116249357

Start: 750696

End: 751808

Strand: Reverse

Name: purR [H]

Synonym: pRL120694

Alternate gene names: 116249357

Gene position: 751808-750696 (Counterclockwise)

Preceding gene: 116249358

Following gene: 116249355

Centisome position: 86.41

GC content: 63.07

Gene sequence:

>1113_bases
TTGAAGTTTTTATGTAAAAACATCGAGTTAGCTATGCCTTCGAAGGCACGACCTCAATTCGTGACGGAAAAAGTGGAACG
CAAAGTCAATCTCAAGGATGTCGCGCGCGATGCTGACGTATCGCTGAGCACAGCCTCGCACGCACTGAACGGAACCGCGC
CGCTGACGGTCGAGGTTCGCGAGAGGGTTCTCGATTCGGCAAAACGCCTAGGTTACCTCGACCGCCGGCGGAAGAAAGCG
ACGATCGCGACCTTACGCGTCCTGCTTCTCGCCATTCCCGACGATGCCGCGCCGGAGAGCGACCTCAACCTGGTGAGCTG
GACGATCCTCAACGGCCTTCGACGGGAGTGCGAGCGCCGCGGGATCAGGATCGTACCCTTTGTCAGCACCGGCCGGCGCA
TCGACGGCGCGCAGGTCAGGCAGCTCGCGCTTGCGGAGCGCGCTGACGGCATCGTGGTCCTGAACGACGATCAGCCGGAA
CTGATCCGAAGCCTCGCCTCCCCCGATATGCCTGTTGTCATCGTCAACGGCGAAGACCCCGCCATGCTGGTCGATACGGT
CACCCCCGAAAACCGCTTCGGGGCGCGGCTCGGCATCGAGCACCTGCTGGCGCTCGGGCATCGCCGCATTCTGCATCTGA
CATGGAAAGGCCGCACGACGATCCAGCGCCGATATGACGGCTTTTCCGATGCCTATCTTGCCGCCCAGCTTCCGGTGCCT
GATAATATGGTCGTCGAGGCCGAGGGATATGAGCCCAGGCACGGCGAGGCTGCCATCAACGCGCTGCTCGACCGCGATGC
CACGGTGAAAGGGGCGACCGCCGTCTTCTGCGCGGCCGACAATCTGGCGCTCGGCTGCCTGAAGGCATTGGCCGATCGTG
ACATACGCGTGCCGGAAGCGATTTCTGTCCTGGGATTCGACGACATCGTCCCCGGCGAATTCAGCCGTCCGCCGCTCTCG
ACGGTCAGCGTTCCCACAGACAAGCTCGGGGCTGCCGCTTTGGCGCTCATCGAACAGAGGCTGATCGCCAACGATCCGCA
GCGGCCGGCCCATCGCCTGGAACTCGGATGCCATCTCGTCCTGCGTGGCAGCATCGCACCGCCGCGCTCCTAG

Upstream 100 bases:

>100_bases
CTTCCTGCAGGCCTATTTCGACCTGCTGCTCAACGGCCTGCTTGCCAAACCTCACTGATGGCCGAATGACGAATAATTCC
GCGCCAAACACGTAGATTTT

Downstream 100 bases:

>100_bases
AGCCTTTCCGGGTTAGATTGAAGCATTCTGCCGGAGCAGGTTTTCGTCAGGGCAAAGGCGATTGGCGAAGGGCATACCCC
CAGGTACGTCCGAGCCGATC

Product: LacI family HTH-type transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MKFLCKNIELAMPSKARPQFVTEKVERKVNLKDVARDADVSLSTASHALNGTAPLTVEVRERVLDSAKRLGYLDRRRKKA
TIATLRVLLLAIPDDAAPESDLNLVSWTILNGLRRECERRGIRIVPFVSTGRRIDGAQVRQLALAERADGIVVLNDDQPE
LIRSLASPDMPVVIVNGEDPAMLVDTVTPENRFGARLGIEHLLALGHRRILHLTWKGRTTIQRRYDGFSDAYLAAQLPVP
DNMVVEAEGYEPRHGEAAINALLDRDATVKGATAVFCAADNLALGCLKALADRDIRVPEAISVLGFDDIVPGEFSRPPLS
TVSVPTDKLGAAALALIEQRLIANDPQRPAHRLELGCHLVLRGSIAPPRS

Sequences:

>Translated_370_residues
MKFLCKNIELAMPSKARPQFVTEKVERKVNLKDVARDADVSLSTASHALNGTAPLTVEVRERVLDSAKRLGYLDRRRKKA
TIATLRVLLLAIPDDAAPESDLNLVSWTILNGLRRECERRGIRIVPFVSTGRRIDGAQVRQLALAERADGIVVLNDDQPE
LIRSLASPDMPVVIVNGEDPAMLVDTVTPENRFGARLGIEHLLALGHRRILHLTWKGRTTIQRRYDGFSDAYLAAQLPVP
DNMVVEAEGYEPRHGEAAINALLDRDATVKGATAVFCAADNLALGCLKALADRDIRVPEAISVLGFDDIVPGEFSRPPLS
TVSVPTDKLGAAALALIEQRLIANDPQRPAHRLELGCHLVLRGSIAPPRS
>Mature_370_residues
MKFLCKNIELAMPSKARPQFVTEKVERKVNLKDVARDADVSLSTASHALNGTAPLTVEVRERVLDSAKRLGYLDRRRKKA
TIATLRVLLLAIPDDAAPESDLNLVSWTILNGLRRECERRGIRIVPFVSTGRRIDGAQVRQLALAERADGIVVLNDDQPE
LIRSLASPDMPVVIVNGEDPAMLVDTVTPENRFGARLGIEHLLALGHRRILHLTWKGRTTIQRRYDGFSDAYLAAQLPVP
DNMVVEAEGYEPRHGEAAINALLDRDATVKGATAVFCAADNLALGCLKALADRDIRVPEAISVLGFDDIVPGEFSRPPLS
TVSVPTDKLGAAALALIEQRLIANDPQRPAHRLELGCHLVLRGSIAPPRS

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=346, Percent_Identity=27.7456647398844, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI1790194, Length=342, Percent_Identity=28.3625730994152, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790369, Length=354, Percent_Identity=25.7062146892655, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788474, Length=337, Percent_Identity=28.1899109792285, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787906, Length=324, Percent_Identity=28.7037037037037, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1789202, Length=302, Percent_Identity=27.4834437086093, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1786540, Length=306, Percent_Identity=27.1241830065359, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789068, Length=313, Percent_Identity=25.5591054313099, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1787580, Length=339, Percent_Identity=24.7787610619469, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI48994940, Length=307, Percent_Identity=24.1042345276873, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 40481; Mature: 40481

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLCKNIELAMPSKARPQFVTEKVERKVNLKDVARDADVSLSTASHALNGTAPLTVEVR
CCCEECCCEEECCCCCCCHHHHHHHHHCCCHHHHHCCCCCEEHHHHHHCCCCCCEEHHHH
ERVLDSAKRLGYLDRRRKKATIATLRVLLLAIPDDAAPESDLNLVSWTILNGLRRECERR
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHC
GIRIVPFVSTGRRIDGAQVRQLALAERADGIVVLNDDQPELIRSLASPDMPVVIVNGEDP
CCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCCCCEEEECCCCC
AMLVDTVTPENRFGARLGIEHLLALGHRRILHLTWKGRTTIQRRYDGFSDAYLAAQLPVP
EEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCCCCEEEEECCCC
DNMVVEAEGYEPRHGEAAINALLDRDATVKGATAVFCAADNLALGCLKALADRDIRVPEA
CCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCHH
ISVLGFDDIVPGEFSRPPLSTVSVPTDKLGAAALALIEQRLIANDPQRPAHRLELGCHLV
HEECCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCEEEE
LRGSIAPPRS
EECCCCCCCC
>Mature Secondary Structure
MKFLCKNIELAMPSKARPQFVTEKVERKVNLKDVARDADVSLSTASHALNGTAPLTVEVR
CCCEECCCEEECCCCCCCHHHHHHHHHCCCHHHHHCCCCCEEHHHHHHCCCCCCEEHHHH
ERVLDSAKRLGYLDRRRKKATIATLRVLLLAIPDDAAPESDLNLVSWTILNGLRRECERR
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHC
GIRIVPFVSTGRRIDGAQVRQLALAERADGIVVLNDDQPELIRSLASPDMPVVIVNGEDP
CCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCCCCEEEECCCCC
AMLVDTVTPENRFGARLGIEHLLALGHRRILHLTWKGRTTIQRRYDGFSDAYLAAQLPVP
EEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHCCCCCCCEEEEECCCC
DNMVVEAEGYEPRHGEAAINALLDRDATVKGATAVFCAADNLALGCLKALADRDIRVPEA
CCEEEEECCCCCCCHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHHHCCCCCCCHH
ISVLGFDDIVPGEFSRPPLSTVSVPTDKLGAAALALIEQRLIANDPQRPAHRLELGCHLV
HEECCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCEEEE
LRGSIAPPRS
EECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA